SwissPalm

O35604 NPC1_MOUSE

General info Cross-references Sequences Orthologs Ortholog sequences Sites Predictions Palmitoyl-proteomes References

Palmitoyl-proteomes

9/23 articles for mouse
3 orthologs

Palmitoyl-proteome experiments

Validation experiments

0 experiment
1 ortholog

Curated sites & Enzymes

0 site from validation experiments^*
5 sites from palmitoyl-proteomes^* Cys³²⁴, Cys⁸⁰⁰, Cys⁷⁹⁹, Cys³²³, Cys³³⁴
No PAT known
No APT known
^*In main isoform only

Sites

Predictions

Predicted

6 cysteines in main isoform

Predictions

Protein general information from UniProt

Protein names

NPC intracellular cholesterol transporter 1; Niemann-Pick C1 protein

Subcellular location

Lysosome membrane; Late endosome membrane (Potential); Lysosome membrane (Probable); Late endosome membrane

Isoforms & protein length

Isoform 1 (main): 1277 aa

Known functions

Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment (PubMed:21896731, PubMed:22048958, PubMed:27551080). Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket (By similarity). May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable). Interacts (via the second lumenal domain) with NPC2 (PubMed:22065762, PubMed:27551080). Interacts with TMEM97. Interacts with TIM1 (By similarity). Detected in liver (at protein level) (PubMed:21896731, PubMed:22048958). Ubiquitous (PubMed:9211850). Detected in adult heart, spleen, lung, liver, skeletal muscle, kidney, testis (PubMed:9211850). A cysteine-rich N-terminal domain and a C-terminal domain containing a di-leucine motif necessary for lysosomal targeting are critical for mobilization of cholesterol from lysosomes. N-glycosylated. Defects in Npc1 cause a lysosomal storage disorder characterized by accumulation of cholesterol in lysosomes and impaired cholesterol homeostasis. Causes age-dependent loss of Purkinje cells, loss of body weight and leads then to ataxia and premature death at a median age of 72 days. Belongs to the patched family. Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment (PubMed:21896731, PubMed:22048958, PubMed:27551080). Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket (By similarity). May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable). cholesterol(in) = cholesterol(out) Interacts (via the second lumenal domain) with NPC2 (PubMed:22065762, PubMed:27551080). Interacts with TMEM97. Interacts with TIM1 (By similarity). Detected in liver (at protein level) (PubMed:21896731, PubMed:22048958). Ubiquitous (PubMed:9211850). Detected in adult heart, spleen, lung, liver, skeletal muscle, kidney, testis (PubMed:9211850). A cysteine-rich N-terminal domain and a C-terminal domain containing a di-leucine motif necessary for lysosomal targeting are critical for mobilization of cholesterol from lysosomes. N-glycosylated. Defects in Npc1 cause a lysosomal storage disorder characterized by accumulation of cholesterol in lysosomes and impaired cholesterol homeostasis. Causes age-dependent loss of Purkinje cells, loss of body weight and leads then to ataxia and premature death at a median age of 72 days. Belongs to the patched family. Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. Interacts with TMEM97 (By similarity). Interacts (via the second lumenal domain) with NPC2 in a cholestrol-dependent manner. Expressed predominantly in perisynaptic astrocytic glial processes. Also expressed in heart, spleen, lung, liver, skeletal muscle, kidney, testis. Activated by the drugs progesterone and U-18666A which block cholesterol transport out of lysosomes and by the lysosomotropic agent NAH4CL. A cysteine-rich N-terminal domain and a C-terminal domain containing a di-leucine motif necessary for lysosomal targeting are critical for mobilization of cholesterol from lysosomes. Belongs to the patched family.

Cross-references

Source	Identifiers
UniProt gene names	Npc1
UniProt secondary AC	O35605, G3X8W9
Refseq protein ID	NP_032746.2
IPI ID	IPI00222830, IPI00395045, IPI00132600

Source	Identifiers
Ensembl protein	ENSMUSP00000025279
Ensembl gene	ENSMUSG00000024413
MGI ID	MGI:1097712

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

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UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Sites	Predicted
F4JTN1	F4JTN1_ARATH	MetaPhors, InParanoid	A. thaliana	F22I13_120, F22I13.120, At4g38350	Patched family protein	0/1	0	0	0	Yes
P87241	RIB2_SCHPO	MetaPhors	S. pombe	SPCC4G3.16, rib2	Diaminohydroxyphosphoribosylamino-pyrimidine deaminase	0/1	0	0	0	No
Q9UHC9	NPCL1_HUMAN	OrthoDB	H. sapiens	NPC1L1	NPC1-like intracellular cholesterol transporter 1	2/22	0	2	0	Yes
G3V7K5	G3V7K5_RAT	MetaPhors, OrthoDB, Ensembl, OMA, InParanoid	R. norvegicus	rCG_41239, Npc1	NPC intracellular cholesterol transporter 1	0/3	0	0	0	Yes
O15118	NPC1_HUMAN	MetaPhors, OrthoDB, OMA, Ensembl, InParanoid	H. sapiens	NPC1	NPC intracellular cholesterol transporter 1	12/22	1	4	2	Yes
F1PB50	F1PB50_CANLF	OrthoDB, OMA, Ensembl, InParanoid	C. familiaris	NPC1	NPC intracellular cholesterol transporter 1	1/1	0	2	0	Yes
B0JYK2	B0JYK2_BOVIN	MetaPhors, OrthoDB, OMA, Ensembl, InParanoid	B. taurus	NPC1	NPC intracellular cholesterol transporter 1	0/2	0	0	0	Yes
Q9VRC9	NPC1B_DROME	MetaPhors, OrthoDB	D. melanogaster	Dmel_CG12092, CG11233, NPC1b, Npc1b, CG12092	NPC intracellular cholesterol transporter 1 homolog 1b	0/1	0	0	0	Yes
Q12200	NPC1_YEAST	OMA, InParanoid	S. cerevisiae S288c	YPL006W, NCR1	NPC intracellular sterol transporter 1-related protein 1	0/1	0	0	0	Yes
F4I9G5	F4I9G5_ARATH	MetaPhors, OMA, InParanoid	A. thaliana	F7F22_1, F7F22.1, At1g42470	Patched family protein	0/1	0	0	0	Yes
Q9VL24	Q9VL24_DROME	OMA, MetaPhors, Ensembl, InParanoid	D. melanogaster	NPC-1, Dmel_CG5722, CG5722, Npc1a, npc1a, NPC1a, Npc1_Dm, npc1, Npc1, NPC1, dnpc1a, dNPC1a, dNPC1, dncp1a, dmNPC1, DmNPC, Dmel\CG5722	Niemann-Pick type C-1a, isoform A	0/1	0	0	0	Yes
Q7YU59	Q7YU59_DROME	InParanoid, OrthoDB	D. melanogaster	CG5722, NPC1, Npc1a	RE56428p	0/1	0	0	0	Yes

Ortholog sequence alignment (CLUSTALW)

Sites (from validation or large-scale experiments)

Isoforms	Position	Probability	Studies	Techniques
O35604	800	NA	SPalmE:903 Collins MO et al. (2017)* SPalmE:855 Shen LF et al. (2017)*	ABE, ARAC
O35604	799	NA	SPalmE:903 Collins MO et al. (2017)*	ABE
O35604	323	NA	SPalmE:903 Collins MO et al. (2017)*	ABE
O35604	324	NA	SPalmE:903 Collins MO et al. (2017)* SPalmE:855 Shen LF et al. (2017)*	ABE, ARAC
O35604	334	NA	SPalmE:903 Collins MO et al. (2017)*	ABE

Cysteine summary and predictions

Pos in alignment	16	25	31	42	63	74	75	97	100	109	113	160	177	184
Subcell. location	U	E	E	E	E	E	E	E	E	E	E	E	E	E
Disulfide	no	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes
PSIPRED v.4.02 prediction	helix	strand	coil	coil	coil	coil	coil	coil	helix	helix	coil	helix	coil	coil
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys¹⁶ HC	Cys²⁵ LC	Cys³¹	Cys⁴²	Cys⁶³	Cys⁷⁴ HC	Cys⁷⁵ HC	Cys⁹⁷ HC	Cys¹⁰⁰	Cys¹⁰⁹	Cys¹¹³	Cys¹⁶⁰	Cys¹⁷⁷	Cys¹⁸⁴

Pos in alignment	227	238	240	243	247	292	323	324	334	346	352	364	468	479
Subcell. location	E	E	E	E	E	C	C	C	C	C	T	T	E	E
Disulfide	yes	yes	yes	yes	yes	no	no	no	no	no	no	no	yes	yes
PSIPRED v.4.02 prediction	coil	strand	coil	coil	coil	helix	coil	coil	helix	helix	helix	helix	strand	coil
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys²²⁷	Cys²³⁸	Cys²⁴⁰	Cys²⁴³ HC	Cys²⁴⁷	Cys²⁹² MC	Cys³²³ HC	Cys³²⁴	Cys³³⁴	Cys³⁴⁶ MC	Cys³⁵²	Cys³⁶⁴	Cys⁴⁶⁸	Cys⁴⁷⁹

Pos in alignment	516	533	645	670	778	799	800	909	914	956	957	967	976	979
Subcell. location	E	E	C	T	T	C	C	E	E	E	E	E	E	E
Disulfide	yes	yes	no	no	no	no	no	yes	yes	yes	yes	yes	yes	yes
PSIPRED v.4.02 prediction	helix	coil	helix	helix	helix	strand	strand	helix	coil	helix	coil	coil	coil	coil
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁵¹⁶	Cys⁵³³	Cys⁶⁴⁵ HC	Cys⁶⁷⁰	Cys⁷⁷⁸	Cys⁷⁹⁹ HC	Cys⁸⁰⁰ HC	Cys⁹⁰⁹	Cys⁹¹⁴	Cys⁹⁵⁶ HC	Cys⁹⁵⁷ HC	Cys⁹⁶⁷	Cys⁹⁷⁶	Cys⁹⁷⁹ LC

Pos in alignment	1011	1118	1127	1160	1167
Subcell. location	E	T	T	T	C
Disulfide	yes	no	no	no	no
PSIPRED v.4.02 prediction	coil	coil	helix	helix	helix
UniProt secondary structure	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys¹⁰¹¹ LC	Cys¹¹¹⁸	Cys¹¹²⁷	Cys¹¹⁶⁰ HC	Cys¹¹⁶⁷

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

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ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	x		HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	Ortholog	in LC [NPC1, NPCL1]	HC: 63 hits LC: 147 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	x		HC: 102 hits LC: 73 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	Ortholog	in LC [NPC1, NPCL1]	HC: 162 hits LC: 218 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	Found	in List	List: 69 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	x		HC HC: 352 hits LC LC: 8 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	Ortholog	in LC [NPC1, NPCL1]	HC: 164 hits LC: 178 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	x		HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	x		Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	Found	in List	List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	Found	in List	List: 436 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Ortholog	in List [NPC1, NPCL1]	List: 1348 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	Ortholog	in List [NPC1, NPCL1]	List: 274 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	Ortholog	in List [NPC1, NPCL1]	List: 226 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	Ortholog	in List [NPC1, NPCL1]	List: 354 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	Ortholog	in List [NPC1, NPCL1]	List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	x		List: 1353 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	Found	in HC	HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	Found	in List	List: 865 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	Found	in HC	HC: 1079 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Found	in List	List: 646 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Found	in LPS treatment	LPS treatment: 646 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Found	in MC	HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Found	in LC	HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in LC [NPC1, NPCL1]	HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	Ortholog	in Trypsin [NPC1, NPCL1]	Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Found	in MC	HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Found	in MC	HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	Ortholog	in List [NPC1, NPCL1]	List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	Ortholog	in List [NPC1, NPCL1]	List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [NPC1, NPCL1]	List: 590 hits
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	Ortholog	in List [NPC1, NPCL1]	List: 2929 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Found	in List	List: 370 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	Ortholog	in Unstimulated [NPC1, NPCL1]	Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	Ortholog	in List [NPC1, NPCL1]	List: 185 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [NPC1, NPCL1]	HC: 1869 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [NPC1, NPCL1]	HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [NPC1, NPCL1]	HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [NPC1, NPCL1]	HC: 600 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [NPC1, NPCL1]	List: 687 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 399 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [F1PB50]	List: 464 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Ortholog	in List [NPC1, NPCL1]	List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Ortholog	in List [NPC1, NPCL1]	List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 199 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1093 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1040 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Found	in HC	HC: 4107 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Found	in HC	HC: 3870 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1378 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1597 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	0	0.0	NA
H. sapiens	22	38	23	60.5	NPC1_HUMAN , NPCL1_HUMAN
M. musculus	23	37	15	40.5	NA
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
S. pombe	1	1	0	0.0	NA
A. thaliana	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	0	0.0	Q9VL24_DROME , NPC1B_DROME
C. familiaris	1	1	1	100.0	F1PB50_CANLF

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