SwissPalm

P00528 SRC64_DROME

General info Cross-references Sequences Orthologs Ortholog sequences Predictions Palmitoyl-proteomes References

Palmitoyl-proteomes

0/1 article for fruit fly
15 orthologs

Palmitoyl-proteome experiments

Validation experiments

0 experiment
12 orthologs

Curated sites & Enzymes

0 site from validation experiments^*
0 site from palmitoyl-proteomes^*
No PAT known
No APT known
^*In main isoform only

Predictions

Predicted

3 cysteines in main isoform

Predictions

Protein general information from UniProt

Protein names

Tyrosine-protein kinase Src64B; Dsrc64; EC 2.7.10.2

Subcellular location

Isoforms & protein length

Isoform 1 (main): 552 aa

Known functions

May play a role in the development of neural tissue and smooth muscle. ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. After the first 8 hours of development, accumulates almost exclusively in neural tissues such as the brain, ventral nerve chord, and eye-antennal disks, and in differentiating smooth muscle. Abundant in embryos and pupae, rare in larvae and adults. Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. May play a role in the development of neural tissue and smooth muscle. ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein] After the first 8 hours of development, accumulates almost exclusively in neural tissues such as the brain, ventral nerve chord, and eye-antennal disks, and in differentiating smooth muscle. Abundant in embryos and pupae, rare in larvae and adults. Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Cross-references

Source	Identifiers
UniProt gene names	CG7524, Src1, Src64B
UniProt secondary AC	Q9VZA2, O18372, A4V1H7
Refseq protein ID	NP_524934.2, NP_001246629.1, NP_001246628.1, NP_001189051.1, NP_001189050.1, NP_001014564.1, NP_001014563.1, NP_001014562.1, NP_001014561.1

Source	Identifiers
Ensembl gene	FBgn0262733

Copy in clipboard UniProt feature viewer

Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

Select all Deselect all

UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Sites	Predicted
Q9SCQ4	Q9SCQ4_ARATH	InParanoid	A. thaliana	At3g50720, T3A5.100	Protein kinase ATN1-like protein	0/1	0	0	0	Yes
Q9LVQ9	Q9LVQ9_ARATH	InParanoid	A. thaliana	At5g66710, MSN2_10, MSN2.10	Protein kinase ATN1-like protein	0/1	0	0	0	Yes
Q9STG5	Q9STG5_ARATH	InParanoid	A. thaliana	At3g46930, T6H20.40	Protein kinase 6-like protein	0/1	0	0	0	No
Q8L6Y9	Q8L6Y9_ARATH	InParanoid	A. thaliana	T20L15_120, T20L15.120, At5g01850	Protein kinase ATN1-like protein	0/1	0	0	0	No
Q9FM43	Q9FM43_ARATH	InParanoid	A. thaliana	At5g40540, MNF13_60, MNF13.60	Protein kinase ATN1	0/1	0	0	0	Yes
Q9FGS7	Q9FGS7_ARATH	InParanoid	A. thaliana	K6A12_4, K6A12.4, At5g50180	At5g50180	0/1	0	0	0	Yes
Q9LT56	Q9LT56_ARATH	InParanoid	A. thaliana	At3g27560, ATN1	AT3g27560/MMJ24_11	0/1	0	0	0	Yes
Q9FIL6	Q9FIL6_ARATH	InParanoid	A. thaliana	K19M22_20, K19M22.20, At5g58950	At5g58950	0/1	0	0	0	No
Q2MHE4	HT1_ARATH	InParanoid	A. thaliana	SUU, F24O1.13, At1g62400, HT1	Serine/threonine/tyrosine-protein kinase HT1	0/1	0	0	0	Yes
Q9ZQ31	STY13_ARATH	InParanoid	A. thaliana	T28I24.9, At2g24360, STY13	Serine/threonine-protein kinase STY13	0/1	0	0	0	Yes
Q9M085	Q9M085_ARATH	InParanoid	A. thaliana	F6E21_90, F6E21.90, At4g31170	AT4G31170 protein	0/1	0	0	0	Yes
P05480	SRC_MOUSE	OrthoDB, Ensembl	M. musculus	Src	Neuronal proto-oncogene tyrosine-protein kinase Src	1/17	0	1	0	Yes
Q04736	YES_MOUSE	OrthoDB, Ensembl	M. musculus	Yes, Yes1	Tyrosine-protein kinase Yes	5/17	1	2	0	Yes
P06239	LCK_HUMAN	OrthoDB, Ensembl	H. sapiens	LCK	Tyrosine-protein kinase Lck	5/17	8	2	1	Yes
P06241	FYN_HUMAN	OrthoDB, Ensembl	H. sapiens	FYN	Tyrosine-protein kinase Fyn	6/17	17	2	4	Yes
P25911	LYN_MOUSE	OrthoDB, Ensembl	M. musculus	Lyn	Tyrosine-protein kinase Lyn	8/17	0	2	2	Yes
P06240	LCK_MOUSE	OrthoDB, Ensembl	M. musculus	Lsk-t, Lck	Proto-oncogene tyrosine-protein kinase LCK	1/17	4	1	4	Yes
F1LM93	YES_RAT	OrthoDB, Ensembl	R. norvegicus	Yes1	Tyrosine-protein kinase Yes	0/2	0	0	0	Yes
P14234	FGR_MOUSE	OrthoDB, Ensembl	M. musculus	Fgr	Tyrosine-protein kinase Fgr	3/17	1	2	0	Yes
P09769	FGR_HUMAN	OrthoDB, Ensembl	H. sapiens	SRC2, FGR	Tyrosine-protein kinase Fgr	1/17	0	1	0	Yes
P12931	SRC_HUMAN	OrthoDB, Ensembl	H. sapiens	SRC1, SRC	Proto-oncogene tyrosine-protein kinase Src	3/17	3	3	0	Yes
Q07014	LYN_RAT	OrthoDB, Ensembl	R. norvegicus	Lyn	Tyrosine-protein kinase Lyn	0/2	0	0	0	Yes
P08631	HCK_HUMAN	OrthoDB, Ensembl	H. sapiens	HCK	Tyrosine-protein kinase HCK	0/17	1	0	1	Yes
P39688	FYN_MOUSE	Ensembl	M. musculus	Fyn	Tyrosine-protein kinase Fyn	9/17	7	2	2	Yes
F1MWN2	F1MWN2_BOVIN	Ensembl	B. taurus	HCK	Tyrosine-protein kinase	0/1	0	0	0	Yes
A5PKG9	A5PKG9_BOVIN	Ensembl	B. taurus	FGR	Tyrosine-protein kinase	0/1	0	0	0	Yes
A0JNB0	FYN_BOVIN	Ensembl	B. taurus	FYN	Tyrosine-protein kinase Fyn	0/1	0	0	0	Yes
F1N261	F1N261_BOVIN	Ensembl	B. taurus	LYN	Tyrosine-protein kinase	0/1	0	0	0	Yes
E1BIM8	E1BIM8_BOVIN	Ensembl	B. taurus	SRC	Tyrosine-protein kinase	0/1	0	0	0	Yes
Q4KM97	Q4KM97_RAT	Ensembl	R. norvegicus	rCG_52180, Blk	Tyrosine-protein kinase	0/2	0	0	0	Yes
Q08DU6	Q08DU6_BOVIN	Ensembl	B. taurus	BLK	Tyrosine-protein kinase	0/1	0	0	0	Yes
P16277	BLK_MOUSE	Ensembl	M. musculus	Blk	Tyrosine-protein kinase Blk	0/17	0	0	0	Yes
Q3ZCM0	Q3ZCM0_BOVIN	Ensembl	B. taurus	LCK	Tyrosine-protein kinase	0/1	0	0	0	Yes
P50527	STE20_SCHPO	MetaPhors	S. pombe	SPBC1604.14c, ste20, pak1, orb2, shk1	Serine/threonine-protein kinase shk1/pak1	0/1	0	0	0	No
P36582	PCK1_SCHPO	MetaPhors	S. pombe	SPAC22H10.01c, SPAC17G8.14c, pck1	Protein kinase C-like 1	0/1	0	0	0	Yes
P41892	CDC7_SCHPO	MetaPhors	S. pombe	SPBC21.06c, cdc7	Cell division control protein 7	0/1	0	0	0	Yes
O59790	ARK1_SCHPO	MetaPhors	S. pombe	SPCC330.16, SPCC320.13c, sex1, ark1	Serine/threonine-protein kinase ark1	1/1	0	1	0	No
Q9LVI6	RLK90_ARATH	MetaPhors	A. thaliana	MEB5.6, At3g17840, RLK902	Probable inactive receptor kinase RLK902	0/1	0	0	0	No
Q8RWL6	STY17_ARATH	MetaPhors	A. thaliana	F8D20.290, At4g35780, STY17	Serine/threonine-protein kinase STY17	0/1	0	0	0	No
Q9FXF2	RKF1_ARATH	MetaPhors	A. thaliana	T3M22.1, F1N18.19, At1g29750, RKF1	Probable LRR receptor-like serine/threonine-protein kinase RFK1	0/1	0	0	0	Yes
Q05609	CTR1_ARATH	MetaPhors	A. thaliana	F17C15_150, At5g03730, CTR1	Serine/threonine-protein kinase CTR1	0/1	0	0	0	No
O04533	LRK52_ARATH	MetaPhors	A. thaliana	F20P5.15, At1g70130, LECRKB1, LECRK52	Putative L-type lectin-domain containing receptor kinase V.2	0/1	0	0	0	Yes
Q01621	LCK_RAT	MetaPhors	R. norvegicus	Lck	Proto-oncogene tyrosine-protein kinase LCK	0/2	0	0	0	Yes
A7MB57	A7MB57_BOVIN	OrthoDB, Ensembl	B. taurus	YES1	Tyrosine-protein kinase	0/1	0	0	0	Yes
Q62662	FRK_RAT	OrthoDB	R. norvegicus	Gask, Frk	Tyrosine-protein kinase FRK	0/2	0	0	0	Yes
E9Q696	E9Q696_MOUSE	Ensembl	M. musculus	Lck	Tyrosine-protein kinase	0/17	0	0	0	Yes
P07948	LYN_HUMAN	Ensembl	H. sapiens	JTK8, LYN	Tyrosine-protein kinase Lyn	4/17	5	3	2	Yes
Q62844	FYN_RAT	Ensembl	R. norvegicus	Fyn	Tyrosine-protein kinase Fyn	0/2	1	0	0	Yes
F6UND7	F6UND7_MOUSE	Ensembl	M. musculus	Hck	Tyrosine-protein kinase	0/17	0	0	0	Yes
P51451	BLK_HUMAN	Ensembl	H. sapiens	BLK	Tyrosine-protein kinase Blk	0/17	0	0	0	Yes
F1LP43	F1LP43_RAT	Ensembl	R. norvegicus	Hck, rCG_37428	Tyrosine-protein kinase	0/2	0	0	0	Yes
Q9WUD9	SRC_RAT	Ensembl	R. norvegicus	Src	Proto-oncogene tyrosine-protein kinase Src	0/2	0	0	0	Yes
Q6P6U0	FGR_RAT	Ensembl	R. norvegicus	Fgr	Tyrosine-protein kinase Fgr	0/2	0	0	0	Yes
J3QRU1	J3QRU1_HUMAN	Ensembl	H. sapiens	YES1	Tyrosine-protein kinase	2/17	0	2	0	Yes
P08103	HCK_MOUSE	Ensembl	M. musculus	Hck	Tyrosine-protein kinase HCK	3/17	1	2	0	Yes
P50545	HCK_RAT	Ensembl	R. norvegicus	Hck	Tyrosine-protein kinase HCK	0/2	0	0	0	Yes
P07947	YES_HUMAN	Ensembl	H. sapiens	YES, YES1	Tyrosine-protein kinase Yes	8/17	2	3	1	Yes

Ortholog sequence alignment (CLUSTALW)

Cysteine summary and predictions

Pos in alignment	5	6	252	259	346	421	435	518
Subcell. location	U	U	U	U	U	U	U	U
Disulfide	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	strand	coil	coil	coil	strand	coil	strand	helix
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁵ HC MC	Cys⁶ HC	Cys²⁵²	Cys²⁵⁹	Cys³⁴⁶	Cys⁴²¹	Cys⁴³⁵	Cys⁵¹⁸ HC

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

Tip: Keep CTRL key pressed down while clicking on column headers to do multiple sorting. This applies to any sortable table on this site.

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	Ortholog	in LC,HC [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	Ortholog	in LC [YES, FGR, FYN, LCK, LYN, HCK, SRC]	HC: 59 hits LC: 105 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	Ortholog	in HC [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	HC: 102 hits LC: 73 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	Ortholog	in HC [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	HC: 162 hits LC: 218 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	Ortholog	in List [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	List: 214 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	Ortholog	in HC [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	HC: 164 hits LC: 178 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	Ortholog	in List [YES, FGR, FYN, LCK, LYN, HCK, SRC]	List: 69 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	Ortholog	in HC [YES, FGR, FYN, LCK, LYN, HCK, SRC]	HC HC: 352 hits LC LC: 8 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	Ortholog	in HC [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	HC HC: 93 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	Ortholog	in List [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	List: 154 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	Ortholog	in HC [YES, FGR, FYN, LCK, LYN, HCK, SRC]	HC HC: 254 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	x		HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	Ortholog	in List [YES, FGR, FYN, LCK, LYN, HCK, SRC]	List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	Ortholog	in List [ARK1]	List: 238 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	Ortholog	in List [YES, FGR, FYN, LCK, LYN, HCK, SRC]	List: 664 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	Ortholog	in List [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	Ortholog	in List [YES, FGR, FYN, LCK, LYN, HCK, SRC]	List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	Ortholog	in List [YES, FGR, FYN, LCK, LYN, HCK, SRC]	List: 436 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Ortholog	in List [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	List: 1348 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	Ortholog	in List [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	List: 274 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	Ortholog	in List [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	List: 226 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	Ortholog	in List [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	List: 354 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	Ortholog	in List [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	List: 354 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	Ortholog	in List [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	List: 1353 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	Ortholog	in MC,HC [YES, FGR, FYN, LCK, LYN, HCK, SRC]	HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	x		HC: 1079 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	Ortholog	in List [YES, FGR, FYN, LCK, LYN, HCK, SRC]	List: 865 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Ortholog	in HC,MC [YES, FGR, FYN, LCK, LYN, HCK, SRC]	HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in Palm. up with LPS [YES, FGR, FYN, LCK, LYN, HCK, SRC]	Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in List [YES, FGR, FYN, LCK, LYN, HCK, SRC]	List: 646 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in LPS treatment [YES, FGR, FYN, LCK, LYN, HCK, SRC]	LPS treatment: 646 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in MC [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Ortholog	in HC,MC,LC [YES, FGR, FYN, LCK, LYN, HCK, SRC]	HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	Ortholog	in List [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	List: 1404 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	Ortholog	in List [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	List: 1394 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	Ortholog	in HC,LC [YES, FGR, FYN, LCK, LYN, HCK, SRC]	HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	Ortholog	in HC [YES, FYN, LCK, LYN, SRC, J3QRU1, FGR]	HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in HC,MC [YES, FGR, FYN, LCK, LYN, HCK, SRC]	HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in HC [YES, FGR, FYN, LCK, LYN, HCK, SRC]	HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	2	3	0	0.0	YES_RAT , LYN_RAT
H. sapiens	17	26	18	69.2	YES_HUMAN , FYN_HUMAN , LCK_HUMAN , LYN_HUMAN , SRC_HUMAN , J3QRU1_HUMAN , FGR_HUMAN
M. musculus	17	25	18	72.0	YES_MOUSE , FGR_MOUSE , FYN_MOUSE , LCK_MOUSE , LYN_MOUSE , HCK_MOUSE , SRC_MOUSE
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
A. thaliana	1	1	0	0.0	NA
S. pombe	1	1	1	100.0	ARK1_SCHPO
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	1	1	0	0.0	NA
D. melanogaster	1	2	0	0.0	NA

References

Authors	Year	Reference title	PMID
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Colquhoun DR et al.	2015	Bioorthogonal mimetics of palmitoyl-CoA and myristoyl-CoA and their subsequent isolation by click chemistry and characterization by mass spectrometry reveal novel acylated host-proteins modified by HIV-1 infection.	25914232
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
Thinon E et al.	2016	Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.	27350074
Kang R et al.	2008	Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.	19092927
Wang J et al.	2019	DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane.	30605677
Yang W et al.	2010	Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes.	19801377
Upadhyay S et al.	2016	Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation.	27879337
Won SJ et al.	2018	Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.	29733200
Pinner AL et al.	2016	Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia.	26876311
Dowal L et al.	2011	Proteomic analysis of palmitoylated platelet proteins.	21813449
Martin BR et al.	2009	Large-scale profiling of protein palmitoylation in mammalian cells.	19137006
Chesarino NM et al.	2014	Chemoproteomics reveals Toll-like receptor fatty acylation.	25371237
Ivaldi C et al.	2012	Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.	22615937
Caballero MC et al.	2016	Identification of new palmitoylated proteins in Toxoplasma gondii.	26825284
Wei X et al.	2014	Insulin-regulated protein palmitoylation impacts endothelial cell function.	24357059
Dumoulin A et al.	2018	S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.	30319353
Forrester MT et al.	2011	Site-specific analysis of protein S-acylation by resin-assisted capture.	21044946
Zhang MM et al.	2013	Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.	23843742
Zhang X et al.	2018	Ultradeep Palmitoylomics Enabled by Dithiodipyridine-Functionalized Magnetic Nanoparticles.	29660268
Peng T et al.	2015	Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.	25575299
Wan J et al.	2013	Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease.	24211138
Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
Sobocińska J et al.	2018	Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.	29217618
Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
Santiago-Tirado FH et al.	2015	A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.	25970403
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
Strassburger K et al.	2019	Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.	30735487
Martin BR et al.	2011	Global profiling of dynamic protein palmitoylation.	22056678
Jones ML et al.	2012	Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis.	22901544
Foe IT et al.	2015	Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.	26468752
Gould NS et al.	2015	Site-Specific Proteomic Mapping Identifies Selectively Modified Regulatory Cysteine Residues in Functionally Distinct Protein Networks.	26165157
Serwa RA et al.	2015	Systems Analysis of Protein Fatty Acylation in Herpes Simplex Virus-Infected Cells Using Chemical Proteomics.	26256475
Hernandez JL et al.	2016	Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.	27030425
Zaręba-Kozioł M et al.	2019	Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins.	31311849
Thinon E et al.	2018	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.	29575903
Morrison E et al.	2015	Quantitative analysis of the human T cell palmitome.	26111759
Marin EP et al.	2012	Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases.	22496122
Merrick BA et al.	2011	Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3.	21785166
Emmer BT et al.	2011	Global analysis of protein palmitoylation in African trypanosomes.	21193548
Ren W et al.	2013	Proteomic analysis of protein palmitoylation in adipocytes.	23599907
Fang C et al.	2016	Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody.	26865113
Roth AF et al.	2006	Global analysis of protein palmitoylation in yeast.	16751107
Hemsley PA et al.	2013	A proteomic approach identifies many novel palmitoylated proteins in Arabidopsis.	23252521