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Protein general information from UniProt

Protein names

Epidermal growth factor receptor, EC 2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Subcellular location

Cell membrane (By similarity); Endoplasmic reticulum membrane; Golgi apparatus membrane (Probable); Nucleus membrane; Endosome; Endosome membrane; Nucleus (Probable); Secreted (By similarity)

Isoforms & protein length

Isoform 1 (main): 1210 aa; Isoform 2 : 405 aa; Isoform 3 : 705 aa; Isoform 4 : 628 aa

Known functions

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Plays a role in enhancing learning and memory performance (By similarity). Isoform 2 may act as an antagonist of EGF action. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins. ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein] Endocytosis and inhibition of the activated EGFR by phosphatases like PTPRJ and PTPRK constitute immediate regulatory mechanisms. Upon EGF-binding phosphorylates EPS15 that regulates EGFR endocytosis and activity. Moreover, inducible feedback inhibitors including LRIG1, SOCS4, SOCS5 and ERRFI1 constitute alternative regulatory mechanisms for the EGFR signaling. Binding of the ligand triggers homo- and/or heterodimerization of the receptor triggering its autophosphorylation. Heterodimer with ERBB2 (PubMed:10805725). Interacts with ERRFI1; inhibits dimerization of the kinase domain and autophosphorylation (PubMed:18046415). Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B (PubMed:10805725). Interacts with GRB2; an adapter protein coupling the receptor to downstream signaling pathways. Interacts with GAB2; involved in signaling downstream of EGFR. Interacts with STAT3; mediates EGFR downstream signaling in cell proliferation. Interacts with RIPK1; involved in NF-kappa-B activation. Interacts (autophosphorylated) with CBL, CBLB and CBLC; involved in EGFR ubiquitination and regulation. Interacts with SOCS5; regulates EGFR degradation through ELOC- and ELOB-mediated ubiquitination and proteasomal degradation. Interacts with PRMT5; methylates EGFR and enhances interaction with PTPN6. Interacts (phosphorylated) with PTPN6; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with COPG1; essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER. Interacts with TNK2; this interaction is dependent on EGF stimulation and kinase activity of EGFR. Interacts with PCNA; positively regulates PCNA (PubMed:17115032). Interacts with PELP1. Interacts with MUC1. Interacts with AP2M1. Interacts with FER. May interact with EPS8; mediates EPS8 phosphorylation. Interacts (via SH2 domains) with GRB2, NCK1 and NCK2 (PubMed:10026169). Interacts with ATX2. Interacts with GAREM1. Interacts (ubiquitinated) with ANKRD13A/B/D; the interaction is direct and may regulate EGFR internalization after EGF stimulation. Interacts with GPER1; the interaction occurs in an estrogen-dependent manner. Interacts (via C-terminal cytoplasmic kinase domain) with ZPR1 (via zinc fingers). Interacts with RNF115 and RNF126 (PubMed:23418353). Interacts with GPRC5A (via its transmembrane domain) (PubMed:25311788). Interacts with FAM83B; positively regulates EGFR inducing its autophosphorylation in absence of stimulation by EGF (PubMed:23912460). Interacts with LAPTM4B; positively correlates with EGFR activation (PubMed:28479384). Interacts with STX19 (PubMed:16420529). Interacts with CD44 (PubMed:23589287). In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER (PubMed:20674546). Endocytosed upon activation by ligand (PubMed:2790960, PubMed:17182860, PubMed:27153536). Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) (PubMed:20551055). Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704, PubMed:17909029). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed:20462955). Plays a role in enhancing learning and memory performance (By similarity). Isoform 2 may act as an antagonist of EGF action. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins. ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein] Endocytosis and inhibition of the activated EGFR by phosphatases like PTPRJ and PTPRK constitute immediate regulatory mechanisms. Upon EGF-binding phosphorylates EPS15 that regulates EGFR endocytosis and activity. Moreover, inducible feedback inhibitors including LRIG1, SOCS4, SOCS5 and ERRFI1 constitute alternative regulatory mechanisms for the EGFR signaling. Up-regulated by NEU3-mediated desialylation of N-linked glycan at Asn-528. Binding of the ligand triggers homo- and/or heterodimerization of the receptor triggering its autophosphorylation. Heterodimer with ERBB2 (PubMed:10805725). Forms a complex with CCDC88A/GIV (via SH2-like regions) and GNAI3 which leads to enhanced EGFR signaling and triggering of cell migration; binding to CCDC88A requires autophosphorylation of the EGFR C-terminal region, and ligand stimulation is required for recruitment of GNAI3 to the complex (PubMed:20462955, PubMed:25187647). Interacts with ERRFI1; inhibits dimerization of the kinase domain and autophosphorylation (PubMed:18046415). Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B (PubMed:10805725). Interacts with GRB2; an adapter protein coupling the receptor to downstream signaling pathways. Interacts with GAB2; involved in signaling downstream of EGFR. Interacts with STAT3; mediates EGFR downstream signaling in cell proliferation. Interacts with RIPK1; involved in NF-kappa-B activation. Interacts (autophosphorylated) with CBL, CBLB and CBLC; involved in EGFR ubiquitination and regulation. Interacts with SOCS5; regulates EGFR degradation through ELOC- and ELOB-mediated ubiquitination and proteasomal degradation. Interacts with PRMT5; methylates EGFR and enhances interaction with PTPN6. Interacts (phosphorylated) with PTPN6; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with COPG1; essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER. Interacts with TNK2; this interaction is dependent on EGF stimulation and kinase activity of EGFR. Interacts with PCNA; positively regulates PCNA (PubMed:17115032). Interacts with PELP1. Interacts with MUC1. Interacts with AP2M1. Interacts with FER. May interact with EPS8; mediates EPS8 phosphorylation. Interacts (via SH2 domains) with GRB2, NCK1 and NCK2 (PubMed:10026169). Interacts with ATXN2. Interacts with GAREM1. Interacts (ubiquitinated) with ANKRD13A/B/D; the interaction is direct and may regulate EGFR internalization after EGF stimulation. Interacts with GPER1; the interaction occurs in an estrogen-dependent manner. Interacts (via C-terminal cytoplasmic kinase domain) with ZPR1 (via zinc fingers). Interacts with RNF115 and RNF126 (PubMed:23418353). Interacts with GPRC5A (via its transmembrane domain) (PubMed:25311788). Interacts with FAM83B; positively regulates EGFR inducing its autophosphorylation in absence of stimulation by EGF (PubMed:23912460). Interacts with LAPTM4B; positively correlates with EGFR activation (PubMed:28479384). Interacts with STX19 (PubMed:16420529). Interacts with CD44 (PubMed:23589287). Interacts with PGRMC1; the interaction requires PGRMC1 homodimerization (PubMed:26988023). Interacts with PIKFYVE (PubMed:17909029). Interacts with NEU3. Interacts with TRAF4 (PubMed:30352854). In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER (PubMed:20674546, PubMed:17909029). Endocytosed upon activation by ligand (PubMed:2790960, PubMed:17182860, PubMed:27153536, PubMed:17909029). Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) (PubMed:20551055). Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Plays a role in enhancing learning and memory performance (By similarity). Isoform 2 may act as an antagonist of EGF action. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins. ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Endocytosis and inhibition of the activated EGFR by phosphatases like PTPRJ and PTPRK constitute immediate regulatory mechanisms. Upon EGF-binding phosphorylates EPS15 that regulates EGFR endocytosis and activity. Moreover, inducible feedback inhibitors including LRIG1, SOCS4, SOCS5 and ERRFI1 constitute alternative regulatory mechanisms for the EGFR signaling. Binding of the ligand triggers homo- and/or heterodimerization of the receptor triggering its autophosphorylation. Heterodimer with ERBB2. Interacts with ERRFI1; inhibits dimerization of the kinase domain and autophosphorylation. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. Interacts with GRB2; an adapter protein coupling the receptor to downstream signaling pathways. Interacts with GAB2; involved in signaling downstream of EGFR. Interacts with STAT3; mediates EGFR downstream signaling in cell proliferation. Interacts with RIPK1; involved in NF-kappa-B activation. Interacts (autophosphorylated) with CBL, CBLB and CBLC; involved in EGFR ubiquitination and regulation. Interacts with SOCS5; regulates EGFR degradation through ELOC- and ELOB-mediated ubiquitination and proteasomal degradation. Interacts with PRMT5; methylates EGFR and enhances interaction with PTPN6. Interacts (phosphorylated) with PTPN6; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with COPG1; essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER. Interacts with TNK2; this interaction is dependent on EGF stimulation and kinase activity of EGFR. Interacts with PCNA; positively regulates PCNA. Interacts with PELP1. Interacts with MUC1. Interacts with AP2M1. Interacts with FER. May interact with EPS8; mediates EPS8 phosphorylation. Interacts (via SH2 domains) with GRB2, NCK1 and NCK2. Interacts with ATX2. Interacts with GAREM1. Interacts (ubiquitinated) with ANKRD13A/B/D; the interaction is direct and may regulate EGFR internalization after EGF stimulation. Interacts with GPER1; the interaction occurs in an estrogen-dependent manner. Interacts (via C-terminal cytoplasmic kinase domain) with ZPR1 (via zinc fingers). Interacts with RNF115 and RNF126. Interacts with GPRC5A (via its transmembrane domain)(PubMed:25311788). Interacts with FAM83B; positively regulates EGFR inducing its autophospharylation in absence of stimulation by EGF (PubMed:23912460). Interacts with LAPTM4B; positively correlates with EGFR activation (PubMed:28479384). In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).

Cross-references

Source	Identifiers
UniProt gene names	EGFR, ERBB, ERBB1, HER1
UniProt secondary AC	O00688, O00732, P06268, Q14225, Q68GS5, Q92795, Q9BZS2, Q9GZX1, Q9H2C9, Q9H3C9, Q9UMD7, Q9UMD8, Q9UMG5
Refseq protein ID	NP_005219.2, NP_958439.1, NP_958440.1, NP_958441.1, XP_005271805.1
IPI ID	IPI00018274, IPI00221346, IPI00221347, IPI00221348, IPI00180111, IPI00098400, IPI00030848

Source	Identifiers
Ensembl protein	ENSP00000275493, ENSP00000342376, ENSP00000345973, ENSP00000413843
Ensembl gene	ENSG00000146648

Enzymes

PAT/APT	Enzyme	Experiments
PAT	ZDH20_HUMAN	SPalmE:749 Runkle KB et al. (2016) SPalmE:750 Runkle KB et al. (2016)

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

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UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Techniques	Sites	Predicted
Q01279	EGFR_MOUSE	OMA, Ensembl, InParanoid, MetaPhors	M. musculus	Egfr	Epidermal growth factor receptor, EC 2.7.10.1	5/23	2	10	Yes
F1PF03	F1PF03_CANLF	Ensembl, OMA, InParanoid	C. familiaris	EGFR	Receptor protein-tyrosine kinase	0/1	0	0	Yes
F1N7F3	F1N7F3_BOVIN	Ensembl, OMA, InParanoid, MetaPhors	B. taurus	EGFR	Receptor protein-tyrosine kinase	0/2	0	0	Yes
Q9SA25	WAKLG_ARATH	InParanoid	A. thaliana	WAKL8, At1g16260, F3O9.6	Wall-associated receptor kinase-like 8, EC 2.7.11.-	0/1	0	0	Yes
Q8RY17	WAKLI_ARATH	InParanoid	A. thaliana	WAKL22, RFO1, At1g79670, F20B17_27	Wall-associated receptor kinase-like 22, EC 2.7.11.-	0/1	0	0	Yes
Q8VYA3	WAKLJ_ARATH	InParanoid	A. thaliana	WAKL10, At1g79680, F20B17.10	Wall-associated receptor kinase-like 10, EC 2.7.11.-	0/1	0	0	Yes
Q7X8C5	WAKLB_ARATH	InParanoid, MetaPhors	A. thaliana	WAKL2, At1g16130, T24D18.21	Wall-associated receptor kinase-like 2, EC 2.7.11.-	0/1	0	0	Yes
Q9S9M3	WAKLC_ARATH	InParanoid	A. thaliana	WAKL3, At1g16140, T24D18.22	Wall-associated receptor kinase-like 3, EC 2.7.11.-	0/1	0	0	Yes
Q9S9M5	WAKLA_ARATH	InParanoid	A. thaliana	WAKL1, At1g16120, T24D18.20	Wall-associated receptor kinase-like 1, EC 2.7.11.-	0/1	0	0	Yes
Q9S9M2	WAKLD_ARATH	InParanoid	A. thaliana	WAKL4, At1g16150, T24D18.23	Wall-associated receptor kinase-like 4, EC 2.7.11.-	0/1	0	0	Yes
Q9S9M1	WAKLE_ARATH	InParanoid	A. thaliana	WAKL5, At1g16160, T24D18.24	Wall-associated receptor kinase-like 5, EC 2.7.11.-	0/1	0	0	Yes
Q9C9L5	WAKLH_ARATH	InParanoid	A. thaliana	WAKL9, At1g69730, T6C23.7	Wall-associated receptor kinase-like 9, EC 2.7.11.-	0/1	0	0	Yes
Q9LN59	WAKLK_ARATH	InParanoid, MetaPhors	A. thaliana	WAKL11, At1g19390, F18O14.11	Putative wall-associated receptor kinase-like 11, EC 2.7.11.-	0/1	0	0	Yes
Q0WNY5	WAKLN_ARATH	InParanoid, MetaPhors	A. thaliana	WAKL18, At4g31110, F6E21.30	Wall-associated receptor kinase-like 18, EC 2.7.11.-	0/1	0	0	Yes
Q9LMT9	WAKLL_ARATH	InParanoid, MetaPhors	A. thaliana	WAKL13, At1g17910, F2H15.13	Putative wall-associated receptor kinase-like 13, EC 2.7.11.-	0/1	0	0	Yes
Q9M092	WAKLM_ARATH	InParanoid	A. thaliana	WAKL17, At4g31100, F6E21.20	Wall-associated receptor kinase-like 17, EC 2.7.11.-	0/1	0	0	Yes
Q8GXQ3	WAKLF_ARATH	InParanoid	A. thaliana	WAKL6, At1g16110, T24D18.19, T24D18_30	Wall-associated receptor kinase-like 6, EC 2.7.11.-	0/1	0	0	Yes
Q9LMN7	WAK5_ARATH	InParanoid	A. thaliana	WAK5, At1g21230, F16F4.9	Wall-associated receptor kinase 5, EC 2.7.11.-	0/1	0	0	Yes
Q9LMP1	WAK2_ARATH	InParanoid	A. thaliana	WAK2, At1g21270, F16F4.5	Wall-associated receptor kinase 2, EC 2.7.11.-	0/1	0	0	Yes
Q39191	WAK1_ARATH	InParanoid	A. thaliana	WAK1, PRO25, At1g21250, F16F4.6	Wall-associated receptor kinase 1, EC 2.7.11.-	0/1	0	0	Yes
Q9LMN8	WAK3_ARATH	InParanoid	A. thaliana	WAK3, At1g21240, F16F4.8	Wall-associated receptor kinase 3, EC 2.7.11.-	0/1	0	0	Yes
Q9LMN6	WAK4_ARATH	InParanoid, MetaPhors	A. thaliana	WAK4, At1g21210, F16F4.10	Wall-associated receptor kinase 4, EC 2.7.11.-	0/1	0	0	Yes
Q9LSV3	WAKLS_ARATH	InParanoid	A. thaliana	WAKL16, At3g25490, MWL2.11	Putative wall-associated receptor kinase-like 16, EC 2.7.11.-	0/1	0	0	Yes
Q9LZM4	WAKLQ_ARATH	InParanoid	A. thaliana	WAKL20, At5g02070, T7H20_120	Wall-associated receptor kinase-like 20, EC 2.7.11.-	0/1	0	0	Yes
Q8RY67	WAKLO_ARATH	InParanoid	A. thaliana	WAKL14, At2g23450, F26B6.10	Wall-associated receptor kinase-like 14, EC 2.7.11.-	0/1	0	0	Yes
Q9M342	WAKLP_ARATH	InParanoid	A. thaliana	WAKL15, At3g53840, F5K20_140	Wall-associated receptor kinase-like 15, EC 2.7.11.-	0/1	0	0	Yes
Q8GYF5	WAKLR_ARATH	InParanoid	A. thaliana	WAKL21, At5g66790, MUD21_3	Wall-associated receptor kinase-like 21, EC 2.7.11.-	0/1	0	0	Yes
Q9C6K9	LRL11_ARATH	InParanoid	A. thaliana	LRK10L-1.1, LRK10L4, At1g25390, F2J7.14	LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.1, EC 2.7.11.1	0/1	0	0	No
F4I3V3	LRL15_ARATH	InParanoid	A. thaliana	LRK10L-1.5, At1g69910, T17F3.6	LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.5, EC 2.7.11.1	0/1	0	0	Yes
D3ZJI4	D3ZJI4_RAT	MetaPhors	R. norvegicus	Tnk1	Tyrosine kinase, non-receptor, 1	0/3	0	0	No
Q8GUH5	Q8GUH5_ARATH	MetaPhors	A. thaliana	At4g33080, F4I10.10, F4I10_10	AGC	0/1	0	0	No
O22558	STY8_ARATH	MetaPhors	A. thaliana	STY8, At2g17700	Serine/threonine-protein kinase STY8, EC 2.7.11.1	0/1	0	0	Yes
Q8RWL6	STY17_ARATH	MetaPhors	A. thaliana	STY17, At4g35780, F8D20.290	Serine/threonine-protein kinase STY17, EC 2.7.11.1	0/1	0	0	No
Q93VD3	CIPKN_ARATH	MetaPhors	A. thaliana	CIPK23, LKS1, PKS17, SnRK3.23, At1g30270, F12P21.6	CBL-interacting serine/threonine-protein kinase 23, EC 2.7.11.1	0/1	0	0	No
Q9FG33	LRKS5_ARATH	MetaPhors	A. thaliana	LECRKS5, At5g06740, MPH15.10	Probable L-type lectin-domain containing receptor kinase S.5, LecRK-S.5, EC 2.7.11.1	0/1	0	0	Yes
Q9M345	LRK42_ARATH	MetaPhors	A. thaliana	LECRK42, LECRKA4, SGC, At3g53810, F5K20.110	L-type lectin-domain containing receptor kinase IV.2, Arabidopsis thaliana lectin-receptor kinase a4, AthlecRK-a4, LecRK-IV.2, EC 2.7.11.1	0/1	0	0	Yes
Q9C823	Y1523_ARATH	MetaPhors	A. thaliana	At1g52310, F19K6.8	C-type lectin receptor-like tyrosine-protein kinase At1g52310, EC 2.7.10.1	0/1	0	0	Yes
Q9FGL5	CEPR1_ARATH	MetaPhors	A. thaliana	CEPR1, XIP1, At5g49660, MNI5.4	Receptor protein-tyrosine kinase CEPR1, EC 2.7.10.1	0/1	0	0	Yes
Q9LX29	ACR4L_ARATH	MetaPhors	A. thaliana	ACR4, At3g59420, F25L23.280	Serine/threonine-protein kinase-like protein ACR4, EC 2.7.11.1	0/1	0	0	Yes
Q9C5S8	CRK5_ARATH	MetaPhors	A. thaliana	CRK5, RLK6, At4g23130, F21P8.20, F7H19.320	Cysteine-rich receptor-like protein kinase 5, Cysteine-rich RLK5, EC 2.7.11.-	0/1	0	0	No
Q8S9L6	CRK29_ARATH	MetaPhors	A. thaliana	CRK29, At4g21410, F18E5.30, T6K22.140	Cysteine-rich receptor-like protein kinase 29, Cysteine-rich RLK29, EC 2.7.11.-	0/1	0	0	Yes
Q9FID5	Y5393_ARATH	MetaPhors	A. thaliana	At5g39030, MXF12.40	Probable receptor-like protein kinase At5g39030, EC 2.7.11.-	0/1	0	0	Yes
Q9FIL7	CRCK1_ARATH	MetaPhors	A. thaliana	CRCK1, At5g58940, K19M22.13	Calmodulin-binding receptor-like cytoplasmic kinase 1, EC 2.7.11.1	0/1	0	0	No
Q9SA26	Q9SA26_ARATH	MetaPhors	A. thaliana	At1g16270, F3O9.7, F3O9_7	F3O9.7 protein	0/1	0	0	No
C0LGG7	Y1534_ARATH	MetaPhors	A. thaliana	At1g53420, F12M16.30	Probable LRR receptor-like serine/threonine-protein kinase At1g53420, EC 2.7.11.1	0/1	0	0	Yes
C0LGD9	Y1756_ARATH	MetaPhors	A. thaliana	At1g07560, F22G5.6	Probable LRR receptor-like serine/threonine-protein kinase At1g07560, EC 2.7.11.1	0/1	0	0	Yes
C0LGF5	RGI5_ARATH	MetaPhors	A. thaliana	RGI5, RGFR5, At1g34110, F12G12.7	LRR receptor-like serine/threonine-protein kinase RGI5, EC 2.7.11.1	0/1	0	0	Yes
O23081	CRK41_ARATH	MetaPhors	A. thaliana	CRK41, At4g00970, A_TM018A10.18, T18A10.9	Cysteine-rich receptor-like protein kinase 41, Cysteine-rich RLK41, EC 2.7.11.-	0/1	0	0	Yes
Q9LPZ3	Y1141_ARATH	MetaPhors	A. thaliana	At1g11410, T23J18.8	G-type lectin S-receptor-like serine/threonine-protein kinase At1g11410, EC 2.7.11.1	0/1	0	0	Yes
F4I3K4	F4I3K4_ARATH	MetaPhors	A. thaliana	At1g56145	Leucine-rich repeat transmembrane protein kinase	0/1	0	0	Yes
Q9FX99	Y1497_ARATH	MetaPhors	A. thaliana	At1g49730, F14J22.6	Probable receptor-like protein kinase At1g49730, EC 2.7.11.1	0/1	0	0	Yes
Q8RX80	CRK18_ARATH	MetaPhors	A. thaliana	CRK18, At4g23260, F21P8.150	Cysteine-rich receptor-like protein kinase 18, Cysteine-rich RLK18, EC 2.7.11.-	0/1	0	0	Yes
Q9SXB3	Y1112_ARATH	MetaPhors	A. thaliana	At1g11280, T28P6.7	G-type lectin S-receptor-like serine/threonine-protein kinase At1g11280, EC 2.7.11.1	0/1	0	0	Yes
D4AAK5	D4AAK5_RAT	MetaPhors	R. norvegicus	Ripk4	Receptor-interacting serine-threonine kinase 4	0/3	0	0	No
P04412	EGFR_DROME	InParanoid, MetaPhors	D. melanogaster	Egfr, c-erbB, DER, top, CG10079	Epidermal growth factor receptor, Egfr, EC 2.7.10.1	0/1	0	0	Yes
G3V6K6	G3V6K6_RAT	InParanoid, MetaPhors, OMA	R. norvegicus	Egfr	Receptor protein-tyrosine kinase	0/3	0	0	Yes

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:627	Bollu LR et al.	2015	Homo sapiens	PC3	Total cell lysate	2BP inhibition, ABE, IF localization, Inhibition of PATs by drugs, Point mutation
SPalmE:749	Runkle KB et al.	2016	Homo sapiens	MDA-MB-231	Total cell lysate	2BP inhibition, ABE, Depletion of PAT by shRNA, IF localization, NH2OH cleavage of thioester bond, PAT localization by IF
SPalmE:750	Runkle KB et al.	2016	Homo sapiens	HEK293T	Total cell lysate	ABE, MS, NH2OH cleavage of thioester bond, PAT overexpression, Point mutation, Substrate truncation
SPalmE:1028	Ali A et al.	2018	Homo sapiens	NSCLC	Total membrane	2BP inhibition, ABE, NH2OH cleavage of thioester bond, Point mutation, Substrate localization by fluorescence tag
SPalmE:1274	Zhou B et al.	2019	Homo sapiens	LNCaP C4-2	Total cell lysate	LB-ABE, NH2OH cleavage of thioester bond

Sites (from validation or large-scale experiments)

Isoforms	Position	Probability	Studies	Techniques
P00533	797	NA	SPalmE:627 Bollu LR et al. (2015) SPalmE:1028 Ali A et al. (2018)	2BP inhibition, ABE, IF localization, Inhibition of PATs by drugs, NH2OH cleavage of thioester bond, Point mutation, Substrate localization by fluorescence tag
P00533	1049	NA	SPalmE:750 Runkle KB et al. (2016) SPalmE:1028 Ali A et al. (2018)	2BP inhibition, ABE, MS, NH2OH cleavage of thioester bond, PAT overexpression, Point mutation, Substrate localization by fluorescence tag, Substrate truncation
P00533	1146	NA	SPalmE:750 Runkle KB et al. (2016) SPalmE:1028 Ali A et al. (2018)	2BP inhibition, ABE, MS, NH2OH cleavage of thioester bond, PAT overexpression, Point mutation, Substrate localization by fluorescence tag, Substrate truncation

Cysteine summary and predictions

Pos in alignment	19	31	58	157	187	190	194	199	207	215	219	223	231	232
Subcell. location	U	E	E	E	E	E	E	E	E	E	E	E	E	E
Disulfide	no	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes
PSIPRED v.4.02 prediction	coil	coil	coil	coil	coil	coil	coil	coil	strand	strand	coil	coil	coil	coil
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	strand	NA	NA	strand	strand	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys¹⁹ HC	Cys³¹	Cys⁵⁸	Cys¹⁵⁷	Cys¹⁸⁷ MC	Cys¹⁹⁰ LC	Cys¹⁹⁴	Cys¹⁹⁹ LC	Cys²⁰⁷	Cys²¹⁵	Cys²¹⁹	Cys²²³	Cys²³¹ HC	Cys²³²
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys¹⁹ HC	Cys³¹	Cys⁵⁸	Cys¹⁵⁷	Cys¹⁸⁷ MC	Cys¹⁹⁰ LC	Cys¹⁹⁴	Cys¹⁹⁹ LC	Cys²⁰⁷	Cys²¹⁵	Cys²¹⁹	Cys²²³	Cys²³¹ HC	Cys²³²
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys¹⁹ HC LC	Cys³¹	Cys⁵⁸	Cys¹⁵⁷	Cys¹⁸⁷ MC	Cys¹⁹⁰ LC	Cys¹⁹⁴	Cys¹⁹⁹ LC	Cys²⁰⁷	Cys²¹⁵	Cys²¹⁹	Cys²²³	Cys²³¹ HC	Cys²³²
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys¹⁹ HC	Cys³¹	Cys⁵⁸	Cys¹⁵⁷	Cys¹⁸⁷ MC	Cys¹⁹⁰ LC	Cys¹⁹⁴	Cys¹⁹⁹ LC	Cys²⁰⁷	Cys²¹⁵	Cys²¹⁹	Cys²²³	Cys²³¹ HC	Cys²³²

Pos in alignment	236	240	248	251	260	264	291	295	307	311	326	329	333	337
Subcell. location	E	E	E	E	E	E	E	E	E	E	E	E	E	E
Disulfide	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes
PSIPRED v.4.02 prediction	helix	coil	coil	coil	strand	coil	strand	coil	strand	coil	strand	coil	coil	strand
UniProt secondary structure	strand	strand	strand	strand	strand	NA	strand	NA	strand	NA	NA	NA	NA	strand
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys²³⁶ LC	Cys²⁴⁰	Cys²⁴⁸	Cys²⁵¹	Cys²⁶⁰	Cys²⁶⁴	Cys²⁹¹	Cys²⁹⁵	Cys³⁰⁷	Cys³¹¹	Cys³²⁶	Cys³²⁹	Cys³³³	Cys³³⁷
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys²³⁶ LC	Cys²⁴⁰	Cys²⁴⁸	Cys²⁵¹	Cys²⁶⁰	Cys²⁶⁴	Cys²⁹¹	Cys²⁹⁵	Cys³⁰⁷	Cys³¹¹	Cys³²⁶	Cys³²⁹	Cys³³³	Cys³³⁷
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys²³⁶ LC	Cys²⁴⁰	Cys²⁴⁸	Cys²⁵¹	Cys²⁶⁰	Cys²⁶⁴	Cys²⁹¹	Cys²⁹⁵	Cys³⁰⁷	Cys³¹¹	Cys³²⁶	Cys³²⁹	Cys³³³	Cys³³⁷
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys²³⁶ LC	Cys²⁴⁰	Cys²⁴⁸	Cys²⁵¹	Cys²⁶⁰	Cys²⁶⁴	Cys²⁹¹	Cys²⁹⁵	Cys³⁰⁷	Cys³¹¹	Cys³²⁶	Cys³²⁹	Cys³³³	Cys³³⁷

Pos in alignment	362	470	499	506	510	515	523	526	535	539	555	558	562	571
Subcell. location	E	E	E	E	E	E	E	E	E	E	E	E	E	E
Disulfide	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes
PSIPRED v.4.02 prediction	strand	strand	helix	coil	coil	coil	strand	coil	strand	coil	strand	coil	helix	strand
UniProt secondary structure	NA	NA	helix	NA	strand	strand	strand	strand	strand	NA	strand	NA	strand	strand
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys³⁶²	Cys⁴⁷⁰	Cys⁴⁹⁹	Cys⁵⁰⁶	Cys⁵¹⁰	Cys⁵¹⁵	Cys⁵²³ MC	Cys⁵²⁶	Cys⁵³⁵	Cys⁵³⁹	Cys⁵⁵⁵	Cys⁵⁵⁸	Cys⁵⁶²	Cys⁵⁷¹
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys³⁶²
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys³⁶²	Cys⁴⁷⁰	Cys⁴⁹⁹	Cys⁵⁰⁶	Cys⁵¹⁰	Cys⁵¹⁵	Cys⁵²³ MC	Cys⁵²⁶	Cys⁵³⁵	Cys⁵³⁹	Cys⁵⁵⁵	Cys⁵⁵⁸	Cys⁵⁶²	Cys⁵⁷¹
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys³⁶²	Cys⁴⁷⁰	Cys⁴⁹⁹	Cys⁵⁰⁶	Cys⁵¹⁰	Cys⁵¹⁵	Cys⁵²³ MC	Cys⁵²⁶	Cys⁵³⁵	Cys⁵³⁹	Cys⁵⁵⁵	Cys⁵⁵⁸	Cys⁵⁶²	Cys⁵⁷¹

Pos in alignment	579	582	591	595	617	620	624	628	636	639	775	781	797	818
Subcell. location	E	E	E	E	E	E	E	E	E	U	C	C	C	C
Disulfide	yes	yes	yes	yes	yes	yes	yes	yes	yes		no	no	no	no
PSIPRED v.4.02 prediction	strand	strand	strand	coil	strand	coil	coil	coil	coil		helix	strand	coil	helix
UniProt secondary structure	strand	strand	strand	NA	strand	NA	NA	NA	NA		NA	strand	strand	helix
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁵⁷⁹	Cys⁵⁸²	Cys⁵⁹¹	Cys⁵⁹⁵	Cys⁶¹⁷	Cys⁶²⁰	Cys⁶²⁴	Cys⁶²⁸	Cys⁶³⁶		Cys⁷⁷⁵	Cys⁷⁸¹	Cys⁷⁹⁷	Cys⁸¹⁸
Pos in isoform 2 CSS-Palm 4.0 PalmPred
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys⁵⁷⁹	Cys⁵⁸²	Cys⁵⁹¹	Cys⁵⁹⁵	Cys⁶¹⁷	Cys⁶²⁰	Cys⁶²⁴			Cys⁶³⁹
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys⁵⁷⁹	Cys⁵⁸²	Cys⁵⁹¹	Cys⁵⁹⁵	Cys⁶¹⁷	Cys⁶²⁰	Cys⁶²⁴

Pos in alignment	939	950	1049	1058	1079	1146	1195	1213
Subcell. location	C	C	C	C	U	C	U	U
Disulfide	no	no	no	no		no
PSIPRED v.4.02 prediction	coil	helix	coil	coil		coil
UniProt secondary structure	strand	helix	NA	NA		NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁹³⁹	Cys⁹⁵⁰	Cys¹⁰⁴⁹	Cys¹⁰⁵⁸		Cys¹¹⁴⁶
Pos in isoform 2 CSS-Palm 4.0 PalmPred
Pos in isoform 3 CSS-Palm 4.0 PalmPred				Cys⁶⁴⁶	Cys⁶⁶⁷		Cys⁶⁸⁶	Cys⁷⁰⁴
Pos in isoform 4 CSS-Palm 4.0 PalmPred

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

Tip: Keep CTRL key pressed down while clicking on column headers to do multiple sorting. This applies to any sortable table on this site.

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	x		HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	x		HC: 162 hits LC: 218 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	x		HC: 102 hits LC: 73 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	x		HC HC: 352 hits LC LC: 8 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	Ortholog	in List [EGFR]	List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	x		HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	x		List: 436 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	x		Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	x		List: 354 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Found	in List	List: 1348 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	x		List: 354 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	x		List: 226 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	Ortholog	in List [EGFR]	List: 780 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	x		List: 274 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	Found	in List	List: 1353 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	x		HC: 1079 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	Ortholog	in List [EGFR]	List: 865 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	x		List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	x		List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		LPS treatment: 646 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		List: 646 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	Found	in List	List: 2929 hits
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 590 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 370 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 600 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		List: 185 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1869 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1093 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 687 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 199 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 399 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1040 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in MC [EGFR]	HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 464 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [EGFR]	HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [EGFR]	HC: 4107 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1597 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1378 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [EGFR]	HC: 3870 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	0	0.0	NA
H. sapiens	22	38	6	15.8	NA
M. musculus	23	37	7	18.9	EGFR_MOUSE
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
A. thaliana	1	1	0	0.0	NA
S. pombe	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	0	0.0	EGFR_DROME
C. familiaris	1	1	0	0.0	NA

References

Authors	Year	Reference title	PMID
Kang R et al.	2008	Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.	19092927
Ren W et al.	2013	Proteomic analysis of protein palmitoylation in adipocytes.	23599907
Marin EP et al.	2012	Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases.	22496122
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
Martin BR et al.	2011	Global profiling of dynamic protein palmitoylation.	22056678
Dowal L et al.	2011	Proteomic analysis of palmitoylated platelet proteins.	21813449
Merrick BA et al.	2011	Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3.	21785166
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
Forrester MT et al.	2011	Site-specific analysis of protein S-acylation by resin-assisted capture.	21044946
Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
Yang W et al.	2010	Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes.	19801377
Martin BR et al.	2009	Large-scale profiling of protein palmitoylation in mammalian cells.	19137006
Roth AF et al.	2006	Global analysis of protein palmitoylation in yeast.	16751107
Hemsley PA et al.	2013	A proteomic approach identifies many novel palmitoylated proteins in Arabidopsis.	23252521
Ivaldi C et al.	2012	Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.	22615937
Wan J et al.	2013	Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease.	24211138
Zhang MM et al.	2013	Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.	23843742
Jones ML et al.	2012	Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis.	22901544
Wei X et al.	2014	Insulin-regulated protein palmitoylation impacts endothelial cell function.	24357059
Serwa RA et al.	2015	Systems Analysis of Protein Fatty Acylation in Herpes Simplex Virus-Infected Cells Using Chemical Proteomics.	26256475
Morrison E et al.	2015	Quantitative analysis of the human T cell palmitome.	26111759
Chesarino NM et al.	2014	Chemoproteomics reveals Toll-like receptor fatty acylation.	25371237
Santiago-Tirado FH et al.	2015	A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.	25970403
Emmer BT et al.	2011	Global analysis of protein palmitoylation in African trypanosomes.	21193548
Colquhoun DR et al.	2015	Bioorthogonal mimetics of palmitoyl-CoA and myristoyl-CoA and their subsequent isolation by click chemistry and characterization by mass spectrometry reveal novel acylated host-proteins modified by HIV-1 infection.	25914232
Foe IT et al.	2015	Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.	26468752
Gould NS et al.	2015	Site-Specific Proteomic Mapping Identifies Selectively Modified Regulatory Cysteine Residues in Functionally Distinct Protein Networks.	26165157
Peng T et al.	2015	Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.	25575299
Bollu LR et al.	2015	Intracellular activation of EGFR by fatty acid synthase dependent palmitoylation.	26378037
Pinner AL et al.	2016	Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia.	26876311
Hernandez JL et al.	2016	Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.	27030425
Fang C et al.	2016	Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody.	26865113
Caballero MC et al.	2016	Identification of new palmitoylated proteins in Toxoplasma gondii.	26825284
Runkle KB et al.	2016	Inhibition of DHHC20-Mediated EGFR Palmitoylation Creates a Dependence on EGFR Signaling.	27153536
Upadhyay S et al.	2016	Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation.	27879337
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
Sobocińska J et al.	2018	Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.	29217618
Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Thinon E et al.	2016	Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.	27350074
Ali A et al.	2018	Fatty acid synthase mediates EGFR palmitoylation in EGFR mutated non-small cell lung cancer.	29449326
Thinon E et al.	2018	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.	29575903
Zhang X et al.	2018	Ultradeep Palmitoylomics Enabled by Dithiodipyridine-Functionalized Magnetic Nanoparticles.	29660268
Won SJ et al.	2018	Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.	29733200
Dumoulin A et al.	2018	S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.	30319353
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Wang J et al.	2019	DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane.	30605677
Strassburger K et al.	2019	Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.	30735487
Zhou B et al.	2019	Low-background Acyl-biotinyl Exchange Largely Eliminates the Co-isolation of Non-S-acylated Proteins and Enables Deep S-acylproteomic Analysis.	31251020
Zaręba-Kozioł M et al.	2019	Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins.	31311849
Wegleiter T et al.	2019	Palmitoylation of BMPR1a regulates neural stem cell fate.	31772009
Morrison E et al.	2020	Dynamic palmitoylation events following T-cell receptor signaling.	32651440
Mariscal J et al.	2020	Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles.	32944167
Gorenberg EL et al.	2022	Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.	35358180
Uzbekova S et al.	2021	Protein Palmitoylation in Bovine Ovarian Follicle.	34769186
Miles MR et al.	2021	Global identification of S-palmitoylated proteins and detection of palmitoylating (DHHC) enzymes in heart.	33636221
Pradhan AJ et al.	2021	Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.	33848465
Ripamonti M et al.	2021	Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions.	33782527
Zaręba-Kozioł M et al.	2021	S-Palmitoylation of Synaptic Proteins as a Novel Mechanism Underlying Sex-Dependent Differences in Neuronal Plasticity.	34200797
Ziemlińska E et al.	2021	Palm Oil-Rich Diet Affects Murine Liver Proteome and <i>S</i>-Palmitoylome.	34884899
Gao J et al.	2022	Proteome-wide identification of palmitoylated proteins in mouse testis.	35477839

P00533 EGFR_HUMAN