SwissPalm

P01112 RASH_HUMAN

General info Cross-references Enzymes Sequences Orthologs Ortholog sequences Validation experiments Sites Predictions Palmitoyl-proteomes References

Palmitoyl-proteomes

18/22 articles for human
9 orthologs

Palmitoyl-proteome experiments

Validation experiments

26 experiments
6 orthologs

Validation experiments

Curated sites & Enzymes

2 sites from validation experiments^* Cys¹⁸¹, Cys¹⁸⁴
0 site from palmitoyl-proteomes^*
DHHC-PATs : ZDHC9_HUMAN, PLAT3_HUMAN
APTs : PPT1_BOVIN, LYPA1_RAT, LYPA2_HUMAN, LYPA1_HUMAN
^*In main isoform only

Sites Enzymes

Predictions

Predicted

2 cysteines in main isoform

Predictions

Protein general information from UniProt

Protein names

GTPase HRas, EC 3.6.5.2; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras [Cleaved into: GTPase HRas, N-terminally processed ]

Subcellular location

Cell membrane (By similarity); Golgi apparatus; Golgi apparatus membrane (Probable); Nucleus (Probable); Cytoplasm (Probable); perinuclear region; Perinuclear region (Probable)

Isoforms & protein length

Isoform 1 (main): 189 aa; Isoform 2 : 170 aa

Known functions

Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151). Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). In its GTP-bound form interacts with PLCE1 (PubMed:11022048). Interacts with TBC1D10C (PubMed:17230191). Interacts with RGL3 (By similarity). Interacts with HSPD1 (By similarity). Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14 (By similarity). Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain) (By similarity). Forms a signaling complex with RASGRP1 and DGKZ (PubMed:11257115). Interacts with RASSF5 (PubMed:18596699). Interacts with PDE6D (PubMed:11980706). Interacts with IKZF3 (PubMed:10369681). Interacts with RACK1 (PubMed:14500341). Interacts with PIK3CG; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6 (By similarity). Interacts with RAPGEF2 (PubMed:10608844, PubMed:11598133). The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. Colocalizes with RACK1 to the perinuclear region. Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151). Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). In its GTP-bound form interacts with PLCE1 (PubMed:11022048). Interacts with TBC1D10C (PubMed:17230191). Interacts with RGL3 (By similarity). Interacts with HSPD1 (By similarity). Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14 (By similarity). Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain) (By similarity). Forms a signaling complex with RASGRP1 and DGKZ (PubMed:11257115). Interacts with RASSF5 (PubMed:18596699). Interacts with PDE6D (PubMed:11980706). Interacts with IKZF3 (PubMed:10369681). Interacts with RACK1 (PubMed:14500341). Interacts with PIK3CG; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6 (By similarity). Interacts with RAPGEF2 (PubMed:10608844, PubMed:11598133). The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. Colocalizes with RACK1 to the perinuclear region. Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151). GTP + H2O = GDP + H(+) + phosphate Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). In its GTP-bound form interacts with PLCE1 (PubMed:11022048). Interacts with TBC1D10C (PubMed:17230191). Interacts with RGL3 (By similarity). Interacts with HSPD1 (By similarity). Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14 (By similarity). Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain) (By similarity). Forms a signaling complex with RASGRP1 and DGKZ (PubMed:11257115). Interacts with RASSF5 (PubMed:18596699). Interacts with PDE6D (PubMed:11980706). Interacts with IKZF3 (PubMed:10369681). Interacts with RACK1 (PubMed:14500341). Interacts with PIK3CG; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6 (By similarity). Interacts with RAPGEF2 (PubMed:10608844, PubMed:11598133). The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. Colocalizes with RACK1 to the perinuclear region.

Cross-references

Source	Identifiers
UniProt gene names	HRAS, HRAS1
UniProt secondary AC	B5BUA0, Q14080, Q6FHV9, Q9BR65, Q9UCE2
Refseq protein ID	NP_001304983.1, NP_001123914.1, NP_005334.1, NP_789765.1
IPI ID	IPI00000006, IPI00741763, IPI00893289, IPI00892901, IPI00892859, IPI00892605, IPI00892512, IPI00873835, IPI00871308, IPI00816702, IPI00816468, IPI00816178, IPI00815701, IPI00791238, IPI00784217, IPI00158697, IPI00028740

Source	Identifiers
Ensembl protein	ENSP00000480686, ENSP00000482366, ENSP00000480317, ENSP00000488757, ENSP00000488225, ENSP00000488296, ENSP00000309845, ENSP00000380722, ENSP00000380723, ENSP00000388246, ENSP00000407586, ENSP00000434023
Ensembl gene	ENSG00000174775, ENSG00000276536

Enzymes

PAT/APT	Enzyme	Experiments
PAT	ZDHC9_HUMAN	SPalmE:75 Swarthout JT et al. (2005)
PAT	PLAT3_HUMAN	SPalmE:817 Wang CH et al. (2014)
APT	PPT1_BOVIN	SPalmE:459 Camp LA et al. (1993)
APT	LYPA1_RAT	SPalmE:785 Duncan JA et al. (1998)
APT	LYPA2_HUMAN	SPalmE:1061 Hernandez JL et al. (2017) SPalmE:1062 Hernandez JL et al. (2017)
APT	LYPA1_HUMAN	SPalmE:1062 Hernandez JL et al. (2017)

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

Select all Deselect all

UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Sites	Predicted
Q61411	RASH_MOUSE	OrthoDB, Ensembl, InParanoid, MetaPhors	M. musculus	Hras1, Hras	GTPase HRas, EC 3.6.5.2	13/23	4	3	2	Yes
Q9CB01	RABF1_ARATH	MetaPhors	A. thaliana	RABF1, ARA-6, RAB5C, At3g54840, F28P10.180	Ras-related protein RABF1, AtRABF1	0/1	1	0	1	Yes
Q38922	RAB1B_ARATH	MetaPhors	A. thaliana	RABB1B, GB2, RAB2C, At4g35860, F4B14.130	Ras-related protein RABB1b, AtRABB1b	0/1	0	0	0	Yes
O23561	RAB1A_ARATH	MetaPhors	A. thaliana	RABB1A, RAB2B, At4g17160, dl4615c, FCAALL.364	Ras-related protein RABB1a, AtRABB1a	0/1	0	0	0	Yes
O49841	RAC2A_ARATH	MetaPhors	A. thaliana	RABC2A, RAB18B, At5g03530, F12E4.310	Ras-related protein RABC2a, AtRABC2a	0/1	0	0	0	Yes
Q9C9U7	RAA6A_ARATH	MetaPhors	A. thaliana	RABA6A, At1g73640, F25P22.5	Ras-related protein RABA6a, AtRABA6a	0/1	0	0	0	Yes
Q9LH50	RAA4D_ARATH	MetaPhors	A. thaliana	RABA4D, At3g12160, F28J15.18, T21B14.2	Ras-related protein RABA4d, AtRABA4d	0/1	0	0	0	Yes
P32883	RASK_MOUSE	OrthoDB	M. musculus	Kras, Kras2	GTPase KRas, EC 3.6.5.2	4/23	0	3	0	Yes
Q04970	RASN_RAT	OrthoDB	R. norvegicus	Nras	GTPase NRas, EC 3.6.5.2	1/3	1	1	0	Yes
F1PHV7	F1PHV7_CANLF	OrthoDB	C. familiaris	NRAS	NRAS proto-oncogene, GTPase	1/1	0	2	0	Yes
G3MXH2	G3MXH2_BOVIN	OrthoDB, Ensembl	B. taurus	HRAS	HRas proto-onco, GTPase	0/2	0	0	0	Yes
P04388	RAS2_DROME	OrthoDB	D. melanogaster	Ras64B, ras2, CG1167	Ras-like protein 2, EC 3.6.5.2	1/1	2	2	5	Yes
P08647	RAS_SCHPO	OMA	S. pombe	ras1, SPAC17H9.09c	Ras-like protein 1	0/1	2	0	1	Yes
F7BIB2	F7BIB2_MOUSE	OMA	M. musculus	Hras, Hras1, mCG_22575	HRAS1	0/23	0	0	0	Yes
J9P550	J9P550_CANLF	OMA, Ensembl	C. familiaris	HRAS	HRas proto-oncogene, GTPase	1/1	0	2	0	Yes
G3N1U2	G3N1U2_BOVIN	OMA, Ensembl, InParanoid, MetaPhors	B. taurus	HRAS	HRas proto-onco, GTPase	1/2	0	2	0	Yes
P20171	RASH_RAT	OMA, OrthoDB, Ensembl, InParanoid, MetaPhors	R. norvegicus	Hras1, Hras	GTPase HRas, EC 3.6.5.2	0/3	3	0	2	Yes
P08646	RAS1_DROME	OMA, OrthoDB, Ensembl, InParanoid, MetaPhors	D. melanogaster	Ras85D, Ras1, CG9375	Ras-like protein 1, Dras1, EC 3.6.5.2	0/1	0	0	0	Yes
P48555	RALA_DROME	OrthoDB	D. melanogaster	Rala, CG2849	Ras-related protein Ral-a, EC 3.6.5.2	1/1	0	2	0	Yes
E1BMX0	E1BMX0_BOVIN	OrthoDB	B. taurus	KRAS	KRAS proto-onco, GTPase	0/2	0	0	0	No
P08644	RASK_RAT	OrthoDB	R. norvegicus	Kras, Kras2	GTPase KRas, EC 3.6.5.2	2/3	0	2	0	Yes

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:74	Dudler T et al.	1996	Homo sapiens	xenopus ovocytes	Total cell lysate	Point mutation, [3H]palmitic acid labeling
SPalmE:75	Swarthout JT et al.	2005	Homo sapiens	Sf9	Total membrane	PAT point mutation, Point mutation, [3H]palmitic acid labeling, [3H]palmitoyl-CoA labeling
SPalmE:327	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	2BP inhibition, ARAC, NH2OH cleavage of thioester bond, Point mutation
SPalmE:378	Cadwallader KA et al.	1994	Chlorocebus aethiops	COS	Total cell lysate	Point mutation, [3H]palmitic acid labeling
SPalmE:459	Camp LA et al.	1993	Spodoptera frugiperda	Sf9	Total cell lysate	HPLC, Thioesterases drug inhibition, [3H]palmitic acid labeling
SPalmE:666	Furuhjelm J et al.	2003	Homo sapiens	HeLa	Total cell lysate	Point mutation, Substrate localization by fluorescence tag, [3H]palmitic acid labeling
SPalmE:774	Tsai FD et al.	2014	Homo sapiens	COS1	Total cell lysate	[3H]palmitic acid labeling
SPalmE:810	Page MJ et al.	1989	Homo sapiens	Sf9	Total cell lysate	NH2OH cleavage of thioester bond, [35S]-Cysteine labeling, [3H]palmitic acid labeling
SPalmE:817	Wang CH et al.	2014	Homo sapiens	HtTA	Total cell lysate	ABE, Co-IP of PAT and substrate, NH2OH cleavage of thioester bond
SPalmE:930	Percher A et al.	2016	Homo sapiens	HEK293T	Total cell lysate	APE, NH2OH cleavage of thioester bond
SPalmE:931	Percher A et al.	2016	Homo sapiens	HeLa	Total cell lysate	APE, NH2OH cleavage of thioester bond
SPalmE:932	Percher A et al.	2016	Homo sapiens	HEK293T	Total cell lysate	APE, Alk16 Labeling, NH2OH cleavage of thioester bond, Point mutation
SPalmE:937	Yap MC et al.	2010	Chlorocebus aethiops	COS7	Total cell lysate	Click-IT Palmitic Acid labeling
SPalmE:938	Yap MC et al.	2010	Homo sapiens	HeLa	Total cell lysate	Click-IT Palmitic Acid labeling
SPalmE:939	Yap MC et al.	2010	Homo sapiens	Jurkat	Total cell lysate	Click-IT Palmitic Acid labeling
SPalmE:940	Yap MC et al.	2010	Mus musculus	Whole organism	NA	Click-IT Palmitic Acid labeling
SPalmE:1060	Charron G et al.	2009	Homo sapiens	Jurkat	Total cell lysate	, Alk12 labeling, Alk16 Labeling, Click-IT Palmitic Acid labeling, NH2OH cleavage of thioester bond
SPalmE:1061	Hernandez JL et al.	2017	Homo sapiens	MCF-10A	Total cell lysate	, ABE, Click chemistry, NH2OH cleavage of thioester bond, Thioesterases overexpression
SPalmE:1062	Hernandez JL et al.	2017	Homo sapiens	HEK293T	Plasma Membrane	ABE, NH2OH cleavage of thioester bond, Thioesterases overexpression
SPalmE:1142	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ABE, ARAC, Alk16 Labeling, NH2OH cleavage of thioester bond
SPalmE:1261	Xiong X et al.	2019	Homo sapiens	U-373MG	Total cell lysate	ABE, NH2OH cleavage of thioester bond
SPalmE:1285	Goloshvili G et al.	2019	Rattus norvegicus	PC12	Total cell lysate	2BP inhibition, ABE, NH2OH cleavage of thioester bond
SPalmE:1384	Plain F et al.	2020	Homo sapiens	HEK293	Total cell lysate	APE, ARAC, NH2OH cleavage of thioester bond, Point mutation
SPalmE:73	Hancock JF et al.	1989	Homo sapiens	COS	Total membrane	Point mutation, Substrate truncation, [3H]palmitic acid labeling
SPalmE:785	Duncan JA et al.	1998	Rattus norvegicus	Liver	Cytosol	[3H]palmitoyl-CoA labeling
SPalmE:1141	Won SJ et al.	2018	Homo sapiens	HEK293T	Total membrane	, 2BP inhibition, ARAC

Sites (from validation or large-scale experiments)

Isoforms	Position	Probability	Studies	Techniques
P01112	181	NA	SPalmE:73 Hancock JF et al. (1989) SPalmE:378 Cadwallader KA et al. (1994) SPalmE:74 Dudler T et al. (1996) SPalmE:75 Swarthout JT et al. (2005) SPalmE:5 Forrester MT et al. (2011)* SPalmE:327 Forrester MT et al. (2011) SPalmE:930 Percher A et al. (2016)	2BP inhibition, APE, ARAC, NH2OH cleavage of thioester bond, PAT point mutation, Point mutation, Substrate truncation, [3H]palmitic acid labeling, [3H]palmitoyl-CoA labeling
P01112	184	NA	SPalmE:73 Hancock JF et al. (1989) SPalmE:378 Cadwallader KA et al. (1994) SPalmE:74 Dudler T et al. (1996) SPalmE:75 Swarthout JT et al. (2005) SPalmE:5 Forrester MT et al. (2011)* SPalmE:327 Forrester MT et al. (2011) SPalmE:930 Percher A et al. (2016)	2BP inhibition, APE, ARAC, NH2OH cleavage of thioester bond, PAT point mutation, Point mutation, Substrate truncation, [3H]palmitic acid labeling, [3H]palmitoyl-CoA labeling

Cysteine summary and predictions

Pos in alignment	51	80	118	186	189	191
Subcell. location	C	C	C	C	C	C
Disulfide	no	no	no	no	no	no
PSIPRED v.4.02 prediction	strand	strand	coil	strand
UniProt secondary structure	strand	strand	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁵¹	Cys⁸⁰	Cys¹¹⁸	Cys¹⁸¹ HC HC	Cys¹⁸⁴ HC HC	Cys¹⁸⁶ MC
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys⁵¹	Cys⁸⁰	Cys¹¹⁸

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

Tip: Keep CTRL key pressed down while clicking on column headers to do multiple sorting. This applies to any sortable table on this site.

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	Found	in HC	HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	Found	in HC	HC: 162 hits LC: 218 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	x		HC: 102 hits LC: 73 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	Found	in HC	HC: 63 hits LC: 147 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	Ortholog	in HC [RASH, RASK]	HC HC: 352 hits LC LC: 8 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	Found	in List	List: 214 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	Found	in List	List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	Found	in HC	HC HC: 93 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	Ortholog	in HC [RASH, RASK]	HC HC: 254 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	Found	in List	List: 154 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	x		HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	Ortholog	in List [RASH, RASK]	List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	Ortholog	in List [RASH, RASK]	List: 436 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	Found	in List	Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	Found	in List	List: 354 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Found	in List	List: 1348 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	Found	in List	List: 354 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	Found	in List	List: 226 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	x		List: 274 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	Ortholog	in MC [RASH, RASK]	HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	Found	in List	List: 1353 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	Found	in List	List: 216 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	Ortholog	in HC [RASH, RASK]	HC: 1079 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	Ortholog	in List [RASN, RASK]	List: 193 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		List: 865 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Ortholog	in LC [RASH, RASK]	HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in MC [RASH, RASK]	HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in LC [RASH, RASK]	HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	Found	in List	List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	Found	in List	List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	Ortholog	in HC [RASH, RASK]	HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [G3N1U2]	List: 52 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in LPS treatment [RASH, RASK]	LPS treatment: 646 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in List [RASH, RASK]	List: 646 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in Palm. up with LPS [RASH, RASK]	Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	Ortholog	in List [RAS2, RALA]	List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	Ortholog	in List [RAS2, RALA]	List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	Found	in List	List: 2929 hits
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	Found	in List	List: 590 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [RASH, RASK]	List: 370 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 600 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	Found	in	Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	Found	in List	List: 185 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1869 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [RASH, RASK]	List: 1093 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Found	in List	List: 687 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Found	in List	List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Found	in List	List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 199 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Found	in List	List: 399 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [RASH, RASK]	List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [RASH, RASK]	List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [RASH, RASK]	List: 1040 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [RASH, RASK]	HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [J9P550, F1PHV7]	List: 464 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [RASH, RASK]	HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 4107 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [RASH, RASK]	List: 1597 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [RASH, RASK]	List: 1378 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 3870 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	2	50.0	RASN_RAT , RASK_RAT
H. sapiens	22	38	27	71.1	NA
M. musculus	23	37	22	59.5	RASH_MOUSE , RASK_MOUSE
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
A. thaliana	1	1	0	0.0	NA
S. pombe	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	1	50.0	G3N1U2_BOVIN
D. melanogaster	1	2	2	100.0	RAS2_DROME , RALA_DROME
C. familiaris	1	1	1	100.0	J9P550_CANLF , F1PHV7_CANLF

References

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