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Protein general information from UniProt

Protein names

HLA class I histocompatibility antigen, B alpha chain; Human leukocyte antigen B, HLA-B

Subcellular location

Membrane (Probable); Cell membrane; Endoplasmic reticulum membrane (By similarity)

Isoforms & protein length

Isoform 1 (main): 362 aa

Known functions

Disease susceptibility is associated with variants affecting the gene represented in this entry. A restricted number of HLA-B*27 subtypes can be associated with ankylosing spondylitis and other B*27-related diseases, and an elevated frequency of the B*27:02 allele in ankylosing spondylitis patients is identified. The allele B*27:07 seems to have a protective role in some populations because it was found only in the healthy controls. Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-B-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells (PubMed:25808313, PubMed:29531227, PubMed:9620674, PubMed:23209413). May also present self-peptides derived from the signal sequence of secreted or membrane proteins, although T cells specific for these peptides are usually inactivated to prevent autoreactivity (PubMed:7743181, PubMed:18991276). Both the peptide and the MHC molecule are recognized by TCR, the peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells (PubMed:29531227, PubMed:9620674, PubMed:24600035). Typically presents intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via constitutive proteasome and IFNG-induced immunoproteasome (PubMed:23209413). Can bind different peptides containing allele-specific binding motifs, which are mainly defined by anchor residues at position 2 and 9 (PubMed:25808313, PubMed:29531227). Allele B*07:02: Displays peptides sharing a common signature motif, namely a Pro residue at position 2 and mainly a Leu anchor residue at the C-terminus (PubMed:7743181). Presents a long peptide (APRGPHGGAASGL) derived from the cancer-testis antigen CTAG1A/NY-ESO-1, eliciting a polyclonal CD8-positive T cell response against tumor cells (PubMed:29531227). Presents viral epitopes derived from HIV-1 gag-pol (TPQDLNTML) and Nef (RPQVPLRPM) (PubMed:25808313). Presents an immunodominant epitope derived from SARS-CoV-2 N/nucleoprotein (SPRWYFYYL) (PubMed:32887977). Displays self-peptides including a peptide derived from the signal sequence of HLA-DPB1 (APRTVALTA) (PubMed:7743181). Allele B*08:01: Presents to CD8-positive T cells viral epitopes derived from EBV/HHV-4 EBNA3 (QAKWRLQTL), eliciting cytotoxic T cell response. Allele B*13:02: Presents multiple HIV-1 epitopes derived from gag (RQANFLGKI, GQMREPRGSDI), nef (RQDILDLWI), gag-pol (RQYDQILIE, GQGQWTYQI) and rev (LQLPPLERL), all having in common a Gln residue at position 2 and mainly hydrophobic amino acids Leu, Ile or Val at the C-terminus. Associated with succesful control of HIV-1 infection. Allele B*18:01: Preferentially presents octomeric and nonameric peptides sharing a common motif, namely a Glu at position 2 and Phe or Tyr anchor residues at the C-terminus (PubMed:14978097, PubMed:23749632, PubMed:18991276). Presents an EBV/HHV-4 epitope derived from BZLF1 (SELEIKRY) (PubMed:23749632). May present to CD8-positive T cells an antigenic peptide derived from MAGEA3 (MEVDPIGHLY), triggering an anti-tumor immune response (PubMed:12366779). May display a broad repertoire of self-peptides with a preference for peptides derived from RNA-binding proteins (PubMed:14978097). Allele B*27:05: Presents to CD8-positive T cells immunodominant viral epitopes derived from HCV POLG (ARMILMTHF), HIV-1 gag (KRWIILGLNK), IAV NP (SRYWAIRTR), SARS-CoV-2 N/nucleoprotein (QRNAPRITF), EBV/HHV-4 EBNA4 (HRCQAIRKK) and EBV/HHV-4 EBNA6 (RRIYDLIEL), confering longterm protection against viral infection (PubMed:19139562, PubMed:18385228, PubMed:15113903, PubMed:9620674, PubMed:32887977). Can present self-peptides derived from cytosolic and nuclear proteins. All peptides carry an Arg at position 2 (PubMed:1922338). The peptide-bound form interacts with NK cell inhibitory receptor KIR3DL1 and inhibits NK cell activation in a peptide-specific way, being particularly sensitive to the nature of the amino acid side chain at position 8 of the antigenic peptide (PubMed:8879234, PubMed:15657948). KIR3DL1 fails to recognize HLA-B*27:05 in complex with B2M and EBV/HHV-4 EBNA6 (RRIYDLIEL) peptide, which can lead to increased activation of NK cells during infection (PubMed:15657948). May present an altered repertoire of peptides in the absence of TAP1-TAP2 and TAPBPL (PubMed:9620674). Allele B*40:01: Presents immunodominant viral epitopes derived from EBV/HHV-4 LMP2 (IEDPPFNSL) and SARS-CoV-2 N/nucleoprotein (MEVTPSGTWL), triggering memory CD8-positive T cell response (PubMed:18991276, PubMed:32887977). Displays self-peptides sharing a signature motif, namely a Glu at position 2 and a Leu anchor residue at the C-terminus (PubMed:18991276). Allele B*41:01: Displays self-peptides sharing a signature motif, namely a Glu at position 2 and Ala or Pro anchor residues at the C-terminus. Allele B*44:02: Presents immunodominant viral epitopes derived from EBV/HHV-4 EBNA4 (VEITPYKPTW) and EBNA6 (AEGGVGWRHW, EENLLDFVRF), triggering memory CD8-positive T cell response (PubMed:9620674, PubMed:18991276). Displays self-peptides sharing a signature motif, namely a Glu at position 2 and Phe, Tyr or Trp anchor residues at the C-terminus (PubMed:18991276). Allele B*45:01: Displays self-peptides sharing a signature motif, namely a Glu at position 2 and Ala or Pro anchor residues at the C-terminus. Allele B*46:01: Preferentially presents nonameric peptides sharing a signature motif, namely Ala and Leu at position 2 and Tyr, Phe, Leu, or Met anchor residues at the C-terminus. The peptide-bound form interacts with KIR2DL3 and inhibits NK cell cytotoxic response in a peptide-specific way. Allele B*47:01: Displays self-peptides sharing a signature motif, namely an Asp at position 2 and Leu or Met anchor residues at the C-terminus. Allele B*49:01: Displays self-peptides sharing a signature motif, namely a Glu at position 2 and Ile or Val anchor residues at the C-terminus. Allele B*50:01: Displays self-peptides sharing a signature motif, namely a Glu at position 2 and Ala or Pro anchor residues at the C-terminus. Allele B*51:01: Presents an octomeric HIV-1 epitope derived from gag-pol (TAFTIPSI) to the public TRAV17/TRBV7-3 TCR clonotype, strongly suppressing HIV-1 replication. Allele B*54:01: Displays peptides sharing a common signature motif, namely a Pro residue at position 2 and Ala anchor residue at the C-terminus. Allele B*55:01: Displays peptides sharing a common signature motif, namely a Pro residue at position 2 and Ala anchor residue at the C-terminus. Allele B*56:01: Displays peptides sharing a common signature motif, namely a Pro residue at position 2 and Ala anchor residue at the C-terminus. Allele B*57:01: The peptide-bound form recognizes KIR3DL1 and inhibits NK cell cytotoxic response. Allele B*67:01: Displays peptides sharing a common signature motif, namely a Pro residue at position 2 and Leu anchor residue at the C-terminus. Heterotrimer that consists of an alpha chain HLA-B, a beta chain B2M and a peptide (peptide-HLA-B-B2M) (PubMed:25808313, PubMed:29531227, PubMed:15657948, PubMed:17057332, PubMed:22020283, PubMed:24600035). Early in biogenesis, HLA-B-B2M dimer interacts with the components of the peptide-loading complex composed of TAPBP, TAP1-TAP2, TAPBPL, PDIA3/ERP57 and CALR (PubMed:9036970, PubMed:9620674, PubMed:26439010, PubMed:26416272). Interacts with TAP1-TAP2 transporter via TAPBP; this interaction is obligatory for the loading of peptide epitopes delivered to the ER by TAP1-TAP2 transporter (PubMed:9036970, PubMed:9620674). Interacts with TAPBPL; TAPBPL binds peptide-free HLA-B-B2M complexes or those loaded with low affinity peptides, likely facilitating peptide exchange for higher affinity peptides (PubMed:26439010). Only optimally assembled peptide-HLA-B-B2M trimer translocates to the surface of antigen-presenting cells, where it interacts with TCR and CD8 coreceptor on the surface of T cells. HLA-B (via polymorphic alpha-1 and alpha-2 domains) interacts with antigen-specific TCR (via CDR1, CDR2 and CDR3 domains) (PubMed:29531227, PubMed:24600035). One HLA-B molecule (mainly via nonpolymorphic alpha-3 domain) interacts with one CD8A homodimer (via CDR-like loop); this interaction insures peptide-HLA-B-B2M recognition by CD8-positive T cells only (PubMed:29531227). Allele B*57:01 interacts (via Bw4 motif) with KIR3DL1 (via Ig-like C2-type domain); this interaction may interfere with peptide binding (PubMed:22020283, PubMed:25480565). Allele B*46:01 interacts with KIR2DL3 (PubMed:28514659). (Microbial infection) Interacts with HTLV-1 accessory protein p12I. The alpha-1 domain is a structural part of the peptide-binding cleft (PubMed:25808313). Residues 101-107 determine Bw4/Bw6 motifs, which serologically distinguish HLA-B alleles. Each HLA-B allele posseses either the Bw4 or Bw6 motif. Only HLA-B alleles bearing the Bw4 epitope are recognized by NK cell inhibitory receptor KIR3DL1 (PubMed:25480565, PubMed:22020283). The alpha-2 domain is a structural part of the peptide-binding cleft (PubMed:25808313). Mediates the interaction with TAP1-TAP2 complex (By similarity). The alpha-3 Ig-like domain mediates the interaction with CD8 coreceptor. The most polymorphic of the mammalian genome. Polymorphic residues encode for alpha-1 and alpha-2 domains of the peptide-binding cleft, where they contribute to variations in peptide binding and TCR recognition among different alleles. The human population is estimated to have millions of HLA-B alleles. But only 17 common HLA-A alleles are considered core alleles, representing all functionally significant variation (polymorphism) in alpha-1 and alpha-2 domains. These are: B*07:02; B*08:01; B*13:02; B*15:01; B*18:01; B*27:05; B*35:01; B*37:01; B*38:01; B*40:01; B*44:02; B*45:01; B*51:01; B*54:01; B*57:01 and B*73:01. Among these, B*07:02; B*15:01; B*18:01; B*37:01; B*51:01; B*54:01; B*57:01 and B*73:01, were likely passed by introgression from archaic to modern humans. Functional alleles of more recent origin (non-core) were derived by recombination (PubMed:28650991). The sequence shown is that of B*07:02. The sequences of core alleles and common representative alleles of serologically distinct allele groups are described as variants of B*07:02. Disease susceptibility is associated with variants affecting the gene represented in this entry. Increased susceptibility to Stevens-Johnson syndrome is conferred by allele B*15:02. There is evidence that HLA-B*51 is associated with susceptibility to Behcet disease (BD). However, it is not certain whether HLA-B*51 itself or a closely linked gene is responsible for susceptibility. The world distribution of HLA-B*51 in healthy people corresponds to the global distribution of BD; in Southern hemisphere countries (Africa, South Pacific, etc.) and in some parts of Europe, the prevalence of HLA-B*51 in healthy people is low or null, corresponding to a low prevalence of BD. The wide variation that exists in the relative risk of HLA-B*51 would support other nongenetic risk factors. The presence of allele B*57:01 is associated with increased susceptibility to abacavir hypersensitivity [MIM:142830] in HIV-1 patients. Allele group B*08 is associated with increased susceptibility to rheumatoid arthritis, where affected individuals have antibodies to cyclic citrullinated peptide (anti-CCP-positive rheumatoid arthritis). Involved in the presentation of foreign antigens to the immune system. Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein (By similarity). Interacts with HTLV-1 accessory protein p12I. Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity). The following alleles of B-7 are known: B*07:02 (B7.2), B*07:03 (BPOT), B*07:04, B*07:05, B*07:06 (B7_L79), B*07:18 and B*07:24. The sequence shown is B*07:02. Belongs to the MHC class I family. Involved in the presentation of foreign antigens to the immune system. Dimer of alpha chain and a beta chain (beta-2-microglobulin). (Microbial infection) Interacts with HTLV-1 accessory protein p12I. Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity). The following alleles of B-7 are known: B*07:02 (B7.2), B*07:03 (BPOT), B*07:04, B*07:05, B*07:06 (B7_L79), B*07:18 and B*07:24. The sequence shown is B*07:02. Belongs to the MHC class I family.

Cross-references

Source	Identifiers
UniProt gene names	HLA-B, HLAB
UniProt secondary AC	Q29638, Q29681, Q29854, Q29861, Q31613, Q5SRJ2, Q9GIX1, Q9TP95
Refseq protein ID	NP_005505.2
IPI ID	IPI00004657, IPI01018006, IPI00973387, IPI00973383, IPI00973381, IPI00973357, IPI00973353, IPI00973339, IPI00973338, IPI00973325, IPI00973319, IPI00973283, IPI00973266, IPI00973256, IPI00973234, IPI00973229, IPI00973224, IPI00973220, IPI00973196, IPI00973185, IPI00973175, IPI00973164, IPI00973150, IPI00973133, IPI00973131, IPI00973124, IPI00973121, IPI00973118, IPI00973113, IPI00973111, IPI00973103, IPI00973099, IPI00973077, IPI00973073, IPI00973040, IPI00973029, IPI00973027, IPI00973022, IPI00973011, IPI00973005, IPI00973000, IPI00972982, IPI00972979, IPI00972978, IPI00972975, IPI00972967, IPI00972957, IPI00972945, IPI00972929, IPI00972924, IPI00970909, IPI00970862, IPI00969369, IPI00969224, IPI00969206, IPI00956574, IPI00956250, IPI00956187, IPI00956094, IPI00956075, IPI00956037, IPI00955896, IPI00955821, IPI00955504, IPI00954298, IPI00954293, IPI00954285, IPI00954280, IPI00954276, IPI00954263, IPI00954256, IPI00954251, IPI00954250, IPI00954248, IPI00954238, IPI00954237, IPI00954233, IPI00954220, IPI00954218, IPI00954211, IPI00954210, IPI00954203, IPI00954198, IPI00954171, IPI00954164, IPI00954158, IPI00954138, IPI00954136, IPI00954133, IPI00954131, IPI00954127, IPI00954122, IPI00954118, IPI00954115, IPI00954113, IPI00954111, IPI00954105, IPI00954103, IPI00954099, IPI00954092, IPI00954079, IPI00954074, IPI00954059, IPI00954046, IPI00954028, IPI00954024, IPI00954020, IPI00954009, IPI00953999, IPI00953948, IPI00953945, IPI00953935, IPI00953932, IPI00953931, IPI00953929, IPI00953919, IPI00953909, IPI00953895, IPI00953676, IPI00953531, IPI00952714, IPI00952683, IPI00947413, IPI00947106, IPI00947032, IPI00946626, IPI00946518, IPI00946328, IPI00946178, IPI00946170, IPI00945921, IPI00945918, IPI00945734, IPI00945411, IPI00945360, IPI00945341, IPI00945170, IPI00944667, IPI00944660, IPI00944653, IPI00944648, IPI00944645, IPI00944639, IPI00944630, IPI00944625, IPI00944620, IPI00944619, IPI00944616, IPI00944615, IPI00944607, IPI00944589, IPI00944586, IPI00944575, IPI00944559, IPI00944552, IPI00944538, IPI00944535, IPI00944532, IPI00944523, IPI00944516, IPI00944512, IPI00944506, IPI00944505, IPI00944503, IPI00944489, IPI00944488, IPI00944485, IPI00944470, IPI00944469, IPI00944466, IPI00944465, IPI00944464, IPI00944460, IPI00944452, IPI00944450, IPI00944449, IPI00944446, IPI00943876, IPI00943861, IPI00943821, IPI00943808, IPI00943795, IPI00943786, IPI00943749, IPI00943739, IPI00943640, IPI00943631, IPI00943624, IPI00943579, IPI00943554, IPI00943549, IPI00943532, IPI00943191, IPI00943134, IPI00943105, IPI00942750, IPI00942453, IPI00942308, IPI00942267, IPI00942250, IPI00941284, IPI00941087, IPI00941067, IPI00941009, IPI00940812, IPI00940774, IPI00940765, IPI00940708, IPI00940689, IPI00940680, IPI00940510, IPI00940328, IPI00940119, IPI00940024, IPI00939860, IPI00939754, IPI00939387, IPI00939234, IPI00939216, IPI00938401, IPI00938398, IPI00938316, IPI00938284, IPI00938241, IPI00938167, IPI00938070, IPI00938016, IPI00937844, IPI00937718, IPI00937486, IPI00937308, IPI00937284, IPI00937228, IPI00937142, IPI00937052, IPI00937048, IPI00936939, IPI00936926, IPI00936789, IPI00936707, IPI00936668, IPI00936538, IPI00936403, IPI00936214, IPI00935772, IPI00935742, IPI00935567, IPI00935564, IPI00935550, IPI00935541, IPI00935490, IPI00935471, IPI00930702, IPI00930699, IPI00930696, IPI00930695, IPI00930686, IPI00930681, IPI00930677, IPI00930666, IPI00930654, IPI00930641, IPI00930636, IPI00930634, IPI00930630, IPI00930627, IPI00930621, IPI00930615, IPI00930596, IPI00930566, IPI00930552, IPI00930516, IPI00930498, IPI00930447, IPI00930436, IPI00930423, IPI00930402, IPI00930381, IPI00930366, IPI00930336, IPI00930322, IPI00930310, IPI00930296, IPI00930283, IPI00930275, IPI00930272, IPI00930251, IPI00930235, IPI00930217, IPI00930212, IPI00930196, IPI00930176, IPI00930173, IPI00930122, IPI00930097, IPI00930080, IPI00930077, IPI00930071, IPI00930058, IPI00930043, IPI00921333, IPI00914977, IPI00910573, IPI00910092, IPI00876935, IPI00843885, IPI00827626, IPI00807558, IPI00789176, IPI00786963, IPI00783289, IPI00783048, IPI00760718, IPI00759516, IPI00739256, IPI00736691, IPI00736090, IPI00735636, IPI00735197, IPI00719334, IPI00719220, IPI00642019, IPI00604738, IPI00604468, IPI00553154, IPI00553028, IPI00552932, IPI00552759, IPI00552739, IPI00552708, IPI00552382, IPI00552310, IPI00552252, IPI00472270, IPI00413352, IPI00384131, IPI00337637, IPI00182703, IPI00026603

Source	Identifiers
Ensembl protein	ENSP00000399168
Ensembl gene	ENSG00000234745

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

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UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Techniques	Sites	Predicted
F1PPX2	F1PPX2_CANLF	Ensembl	C. familiaris	DLA-79	Uncharacterized protein	0/1	0	0	No
Q31093	Q31093_MOUSE	Ensembl, MetaPhors	M. musculus	H2-M3, H-2M3	Histocompatibility 2, M region locus 3	0/23	0	0	Yes
O19443	O19443_MOUSE	Ensembl	M. musculus	H2-M10.1, M10	Histocompatibility 2, M region locus 10.1	0/23	0	0	Yes
F6U8V3	F6U8V3_MOUSE	Ensembl	M. musculus	H2-M11	Histocompatibility 2, M region locus 11	0/23	0	0	No
F1MWX8	F1MWX8_BOVIN	Ensembl, MetaPhors	B. taurus	BOLA-N	MHC Class I JSP.1	0/2	0	0	No
P14429	HA17_MOUSE	Ensembl, MetaPhors	M. musculus	H2-Q7	H-2 class I histocompatibility antigen, Q7 alpha chain	0/23	0	0	No
Q85ZW7	Q85ZW7_MOUSE	Ensembl	M. musculus	H2-M10.5	Histocompatibility 2, M region locus 10.5	0/23	0	0	Yes
F6T1I5	F6T1I5_MOUSE	Ensembl, MetaPhors	M. musculus	H2-T10	Histocompatibility 2, T region locus 10	0/23	0	0	Yes
E1BI37	E1BI37_BOVIN	Ensembl, MetaPhors	B. taurus	BOLA	MHC class I heavy chain	0/2	0	0	No
P06339	HA15_MOUSE	Ensembl, MetaPhors	M. musculus	H2-T23	H-2 class I histocompatibility antigen, D-37 alpha chain	0/23	0	0	No
A7VMS2	A7VMS2_MOUSE	Ensembl, MetaPhors	M. musculus	Gm11127, H2-T15	MHC classIb T15	0/23	0	0	Yes
Q6W9J6	Q6W9J6_MOUSE	Ensembl	M. musculus	H2-M2	Histocompatibility 2, M region locus 2	0/23	0	0	No
F1N4Y5	F1N4Y5_BOVIN	Ensembl, MetaPhors	B. taurus	BOLA-NC1	Non-classical MHC class I antigen	0/2	0	0	No
A7YWH4	A7YWH4_BOVIN	Ensembl, MetaPhors	B. taurus	BOLA-A, BoLA	BoLA protein	0/2	0	0	No
E9PX63	E9PX63_MOUSE	Ensembl	M. musculus	H2-Q1	Histocompatibility 2, Q region locus 1	0/23	0	0	No
F1MWX3	F1MWX3_BOVIN	Ensembl, MetaPhors	B. taurus	BOLA	Ig-like domain-containing protein	0/2	0	0	No
P79568	P79568_MOUSE	Ensembl, MetaPhors	M. musculus	H2-Q6	Class Ib MHC antigen Qa-2	0/23	0	0	No
F7CXU4	F7CXU4_MOUSE	Ensembl	M. musculus	H2-M1	Histocompatibility 2, M region locus 1	0/23	0	0	No
P01898	HA10_MOUSE	Ensembl, MetaPhors	M. musculus	H2-Q10	H-2 class I histocompatibility antigen, Q10 alpha chain	0/23	0	0	No
Q0WXH6	Q0WXH6_MOUSE	Ensembl, MetaPhors	M. musculus	Gm7030, EG630294, Gm11127, H2-T9	MHC class Ib T9	0/23	0	0	Yes
F1N3I2	F1N3I2_BOVIN	Ensembl, OrthoDB, MetaPhors	B. taurus	LOC788634	Uncharacterized protein	0/2	0	0	Yes
Q8HWB2	Q8HWB2_MOUSE	Ensembl, OrthoDB, MetaPhors	M. musculus	H2-Q4, H2-Gs10, H2-gs10, H2-Q1	Histocompatibility 2, Q region locus 4	1/23	1	0	No
Q9BCZ1	Q9BCZ1_MOUSE	Ensembl	M. musculus	H2-T22	H2-T22 protein	0/23	0	0	Yes
Q85ZW9	Q85ZW9_MOUSE	Ensembl	M. musculus	H2-M10.2	Histocompatibility 2, M region locus 10.2	0/23	0	0	Yes
F1MPL6	F1MPL6_BOVIN	Ensembl, MetaPhors	B. taurus	BOLA-NC1	Non-classical MHC class I antigen	0/2	0	0	No
Q85ZW6	Q85ZW6_MOUSE	Ensembl	M. musculus	H2-M10.3	Histocompatibility 2, M region locus 10.3	0/23	0	0	Yes
Q85ZW8	Q85ZW8_MOUSE	Ensembl	M. musculus	H2-M10.4	Histocompatibility 2, M region locus 10.4	0/23	0	0	Yes
F1MGC3	F1MGC3_BOVIN	Ensembl	B. taurus	LOC512672	Ig-like domain-containing protein	0/2	0	0	No
A7VMS3	A7VMS3_MOUSE	Ensembl	M. musculus	H2-M5	Histocompatibility 2, M region locus 5	0/23	0	0	No
F1MZQ5	F1MZQ5_BOVIN	Ensembl, MetaPhors	B. taurus	BOLA	Ig-like domain-containing protein	0/2	0	0	Yes
Q85ZW5	Q85ZW5_MOUSE	Ensembl	M. musculus	H2-M10.6	Histocompatibility 2, M region locus 10.6	0/23	0	0	Yes
P01899	HA11_MOUSE	Ensembl, MetaPhors	M. musculus	H2-D1	H-2 class I histocompatibility antigen, D-B alpha chain, H-2D	1/23	1	0	No
E1BD63	E1BD63_BOVIN	Ensembl, MetaPhors	B. taurus	LOC616942	Ig-like domain-containing protein	0/2	0	0	No
F6TIX5	F6TIX5_MOUSE	Ensembl	M. musculus	H2-T3	H-2 class I histocompatibility antigen, TLA	0/23	0	0	No
F8VQG4	F8VQG4_MOUSE	Ensembl	M. musculus	H2-T24	Histocompatibility 2, T region locus 24	0/23	0	0	No
G3UXE9	G3UXE9_MOUSE	Ensembl, MetaPhors	M. musculus	Gm8909	Predicted gene 8909	0/23	0	0	No
Q3TH01	Q3TH01_MOUSE	Ensembl	M. musculus	H2-K1	H-2 class I histocompatibility antigen, K-K alpha chain	0/23	0	0	No
P01901	HA1B_MOUSE	Ensembl, MetaPhors	M. musculus	H2-K1, H2-K	H-2 class I histocompatibility antigen, K-B alpha chain, H-2K	3/23	2	1	No
Q10302	YD49_SCHPO	MetaPhors	S. pombe	SPAC22H10.09	Uncharacterized protein C22H10.09	0/1	0	0	No
P14432	HA1T_MOUSE	MetaPhors	M. musculus	H2-T3	H-2 class I histocompatibility antigen, TLA	0/23	0	0	Yes
V9GXR0	V9GXR0_MOUSE	MetaPhors	M. musculus	H2-Bl	Histocompatibility 2, blastocyst	0/23	0	0	No
Q9VFU7	Q9VFU7_DROME	MetaPhors	D. melanogaster	side-IV, 14372, BP1073, CT34011, Dmel\CG14372, CG14372, Dmel_CG14372	Sidestep IV, isoform C	0/1	0	0	Yes
P14430	HA18_MOUSE	MetaPhors	M. musculus	H2-Q8	H-2 class I histocompatibility antigen, Q8 alpha chain	0/23	0	0	No
Q4KN81	Q4KN81_MOUSE	OrthoDB, Ensembl, MetaPhors	M. musculus	H2-Q2, Gm11132	Histocompatibility 2, Q region locus 2	0/23	0	0	No
M0R866	M0R866_RAT	OrthoDB, Ensembl	R. norvegicus	RT1-M3-1	RT1 class Ib, locus M3, gene 1	0/3	0	0	No
F6RF06	F6RF06_BOVIN	OrthoDB, Ensembl, MetaPhors	B. taurus	BOLA	MHC class I heavy chain	0/2	0	0	No
F1PE32	F1PE32_CANLF	OrthoDB, Ensembl	C. familiaris	DLA88	Uncharacterized protein	0/1	0	0	No
Q6MG05	Q6MG05_RAT	Ensembl	R. norvegicus	RT1-T24-3, RT1-T24-1, 2310ex4-5, 2331ex4-5, 2361ex4-5, Flot1, Gnl1, Kiaa1949, Rpl41-ps, Rps2-ps1, Rps2-ps2, RT1-O2, RT1-O3, RT1-S2, RT1-S3, RT1-T24-2, Tubb5	RT1 class I, T24, gene 1	0/3	0	0	Yes
Q6MG01	Q6MG01_RAT	Ensembl	R. norvegicus	RT1-N3, 2310ex4-5, 2331ex4-5, 2361ex4-5, Flot1, Gnl1, Kiaa1949, Rpl41-ps, Rps2-ps1, Rps2-ps2, RT1-O2, RT1-O3, RT1-S2, RT1-S3, RT1-T24-1, RT1-T24-2, Tubb5	RT1 class I, N3	0/3	0	0	Yes
Q6MG34	Q6MG34_RAT	Ensembl, MetaPhors	R. norvegicus	RT1-CE10, Aif1, Cdk2ap1-ps2, Cdk2ap1-ps3, Cdk2ap1-ps4, Cdk2ap1-ps5, Cdk2ap1-ps6, E230034O05Rik, LOC224733l-3, LOC224733l-4, LOC224733l-5, LOC224733l-6, LOC224733l-7, LOC224733l-8, Lst1, RT1-CE12, RT1-CE3, RT1-CE5, RT1-CE6, RT1-CE8, RT1-CE9, Sacm2l-ps3, Sacm2l-ps4, Sacm2l-ps5, Sacm2l-ps6, Sacm2l-ps7, Tnf	RT1 class I, CE10	0/3	0	0	No
Q6MFZ8	Q6MFZ8_RAT	Ensembl	R. norvegicus	RT1-N2, 2310ex4-5, 2331ex4-5, 2361ex4-5, Flot1, Gnl1, Kiaa1949, Rpl41-ps, Rps2-ps1, Rps2-ps2, RT1-O2, RT1-O3, RT1-S2, RT1-S3, RT1-T24-1, RT1-T24-2, Tubb5, rCG_41803	RCG41803, isoform CRA_a	0/3	0	0	No
G3V627	G3V627_RAT	Ensembl	R. norvegicus	RT1-M3-1, RT1-M3, rCG_58606	RT1 class Ib, locus M3, gene 1	0/3	0	0	No
Q6MFY3	Q6MFY3_RAT	Ensembl	R. norvegicus	RT1-M6-2, 9430032L10Rik, Gabbr1, Mog, Olr1735_predicted, Olr1736_predicted, Olr1737_predicted, Olr1738_predicted, Olr1739_predicted, Olr1742_predicted, Olr1743_predicted, Olr1744_predicted, Olr1745_predicted, Olr1746_predicted, Olr1748_predicted, Olr1749_predicted, Olr1750_predicted, Rps15-ps, RT1-M3, RT1-M3-2, RT1-M6-1, Ubd, Zfp57, Znrd1	RT1 class I, M6, gene 2	0/3	0	0	No
F1PE31	F1PE31_CANLF	Ensembl	C. familiaris	LOC100685196	Uncharacterized protein	0/1	0	0	No
F1PEZ8	F1PEZ8_CANLF	Ensembl	C. familiaris	DLA-12	Uncharacterized protein	0/1	0	0	No
Q861Q1	Q861Q1_RAT	Ensembl	R. norvegicus	Lst1, Cdk2ap1-ps6, RT1-CE4, Aif1, Cdk2ap1-ps2, Cdk2ap1-ps3, Cdk2ap1-ps4, Cdk2ap1-ps5, E230034O05Rik, LOC224733l-3, LOC224733l-4, LOC224733l-5, LOC224733l-6, LOC224733l-7, LOC224733l-8, RT1-C/E4, RT1-CE12, RT1-CE3, RT1-CE5, RT1-CE6, RT1-CE8, RT1-CE9, Sacm2l-ps3, Sacm2l-ps4, Sacm2l-ps5, Sacm2l-ps6, Sacm2l-ps7, Tnf	MHC class I protein	0/3	0	0	No
Q6W9J5	Q6W9J5_RAT	Ensembl	R. norvegicus	RT1-M2	MHC class Ib antigen	0/3	0	0	No
Q6MGB8	Q6MGB8_RAT	Ensembl	R. norvegicus	RT1-A2n, RT1-A2, AA926063, B3galt4, Cdk2ap1-ps1, Col11a2, Daxx, Hsd17b8, Kifc1, LOC224733l-1, LOC224733l-2, Phf1, Rps25-ps1, RT1-A1, RT1-A3, RT1-DOa, RT1-Hb, RT1-Ke4, Rxrb, Sacm2l-ps1, Sacm2l-ps2, Sec61-ps, Syngap1	RT1 class I, A2	0/3	0	0	No
F1LSU0	F1LSU0_RAT	Ensembl, MetaPhors	R. norvegicus	RT1-M4	RT1 class Ib, locus M4	0/3	0	0	No
M0R4E9	M0R4E9_RAT	Ensembl	R. norvegicus	AABR07044397.1, RT1-CE3	Ig-like domain-containing protein	0/3	0	0	No
F1LQF2	F1LQF2_RAT	Ensembl, MetaPhors	R. norvegicus	RT1-S3	RT1 class Ib, locus S3	0/3	0	0	No
Q5WR11	Q5WR11_CANLF	Ensembl	C. familiaris	DLA-64	MHC class I antigen	0/1	0	0	No
Q4G002	Q4G002_RAT	Ensembl	R. norvegicus	RT1-T24-4, H2-T24	Histocompatibility 2, T region locus 24	0/3	0	0	Yes
F1M115	F1M115_RAT	Ensembl	R. norvegicus	LOC120093125, LOC100364500	Ig-like domain-containing protein	0/3	0	0	Yes
Q95II0	Q95II0_RAT	Ensembl	R. norvegicus	LOC100364956, RT1-CE5, RT1-E2	MHC class Ib antigen	0/3	0	0	No
Q6MFZ9	Q6MFZ9_RAT	Ensembl	R. norvegicus	RT1-S2, 2310ex4-5, 2331ex4-5, 2361ex4-5, Flot1, Gnl1, Kiaa1949, Rpl41-ps, Rps2-ps1, Rps2-ps2, RT1-O2, RT1-O3, RT1-S3, RT1-T24-1, RT1-T24-2, Tubb5	RT1 class I, S2	0/3	0	0	Yes
F1M9F3	F1M9F3_RAT	Ensembl	R. norvegicus	RT1-CE16	RT1 class I, locus CE16	0/3	0	0	No
M0R642	M0R642_RAT	Ensembl	R. norvegicus	RT1-T24-3	RT1 class I, locus T24, gene 3	0/3	0	0	No
F1MAQ9	F1MAQ9_RAT	Ensembl	R. norvegicus	RT1-CE7	RT1 class I, locus CE7	0/3	0	0	No
F1MAR7	F1MAR7_RAT	Ensembl, MetaPhors	R. norvegicus	RT1-M5	RT1 class Ib, locus M5	0/3	0	0	Yes
E9PSS8	E9PSS8_RAT	Ensembl	R. norvegicus	RT1-A1	RT1 class Ia, locus A1	0/3	0	0	No
E9PTZ7	E9PTZ7_RAT	Ensembl	R. norvegicus	RT1-CE1	RT1 class I, locus1	0/3	0	0	No
F7FP91	F7FP91_RAT	OrthoDB	R. norvegicus	RT1-CE5, LOC100364956	Protein RT1-CE5	0/3	0	0	No

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:310	Gruda R et al.	2007	Homo sapiens	LCL 721.221	Total cell lysate	Point mutation, [3H]palmitic acid labeling
SPalmE:783	Kaufman JF et al.	1984	Homo sapiens	JY	Total cell lysate	NH2OH cleavage of thioester bond, TLC, [35S]-Cysteine labeling, [3H]palmitic acid labeling

Sites (from validation or large-scale experiments)

Isoforms	Position	Probability	Studies	Techniques
P01889	349	NA	SPalmE:310 Gruda R et al. (2007)	Point mutation, [3H]palmitic acid labeling
P01889	332	NA	SPalmE:310 Gruda R et al. (2007)	Point mutation, [3H]palmitic acid labeling

Cysteine summary and predictions

Pos in alignment	125	188	227	283	332	349
Subcell. location	E	E	E	E	T	C
Disulfide	yes	yes	yes	yes	no	no
PSIPRED v.4.02 prediction	strand	helix	strand	strand	strand	coil
UniProt secondary structure	strand	helix	strand	strand	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys¹²⁵	Cys¹⁸⁸	Cys²²⁷	Cys²⁸³	Cys³³² HC MC	Cys³⁴⁹

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

Tip: Keep CTRL key pressed down while clicking on column headers to do multiple sorting. This applies to any sortable table on this site.

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	Found	in HC	HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	Found	in LC	HC: 162 hits LC: 218 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	Found	in LC	HC: 102 hits LC: 73 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	Ortholog	in HC [HA1B, Q8HWB2, HA11]	HC HC: 352 hits LC LC: 8 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	Found	in List	List: 214 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	Found	in HC	HC HC: 93 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	x		HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	x		List: 436 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	Found	in List	Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	x		List: 354 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Found	in List	List: 1348 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	x		List: 354 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	Found	in List	List: 226 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	Ortholog	in List [HA1B, Q8HWB2, HA11]	List: 780 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	x		List: 274 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	Found	in List	List: 1353 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	x		HC: 1079 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		List: 865 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Ortholog	in MC [HA1B, Q8HWB2, HA11]	HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	x		List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	x		List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		LPS treatment: 646 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		List: 646 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	Found	in List	List: 2929 hits
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 590 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 370 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 600 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	Found	in	Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	Found	in List	List: 185 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1869 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1093 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 687 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 199 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 399 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1040 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [HA1B, Q8HWB2, HA11]	HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 464 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in MC [HA1B, Q8HWB2, HA11]	HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [HA1B, Q8HWB2, HA11]	HC: 4107 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1597 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1378 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 3870 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	0	0.0	NA
H. sapiens	22	38	16	42.1	NA
M. musculus	23	37	6	16.2	HA1B_MOUSE , Q8HWB2_MOUSE , HA11_MOUSE
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
A. thaliana	1	1	0	0.0	NA
S. pombe	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	0	0.0	NA
C. familiaris	1	1	0	0.0	NA

References

Authors	Year	Reference title	PMID
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Ren W et al.	2013	Proteomic analysis of protein palmitoylation in adipocytes.	23599907
Marin EP et al.	2012	Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases.	22496122
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
Martin BR et al.	2011	Global profiling of dynamic protein palmitoylation.	22056678
Dowal L et al.	2011	Proteomic analysis of palmitoylated platelet proteins.	21813449
Merrick BA et al.	2011	Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3.	21785166
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
Forrester MT et al.	2011	Site-specific analysis of protein S-acylation by resin-assisted capture.	21044946
Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
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Martin BR et al.	2009	Large-scale profiling of protein palmitoylation in mammalian cells.	19137006
Roth AF et al.	2006	Global analysis of protein palmitoylation in yeast.	16751107
Hemsley PA et al.	2013	A proteomic approach identifies many novel palmitoylated proteins in Arabidopsis.	23252521
Ivaldi C et al.	2012	Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.	22615937
Wan J et al.	2013	Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease.	24211138
Zhang MM et al.	2013	Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.	23843742
Jones ML et al.	2012	Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis.	22901544
Wei X et al.	2014	Insulin-regulated protein palmitoylation impacts endothelial cell function.	24357059
Gruda R et al.	2007	Intracellular cysteine residues in the tail of MHC class I proteins are crucial for extracellular recognition by leukocyte Ig-like receptor 1.	17785801
Serwa RA et al.	2015	Systems Analysis of Protein Fatty Acylation in Herpes Simplex Virus-Infected Cells Using Chemical Proteomics.	26256475
Morrison E et al.	2015	Quantitative analysis of the human T cell palmitome.	26111759
Chesarino NM et al.	2014	Chemoproteomics reveals Toll-like receptor fatty acylation.	25371237
Santiago-Tirado FH et al.	2015	A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.	25970403
Emmer BT et al.	2011	Global analysis of protein palmitoylation in African trypanosomes.	21193548
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Foe IT et al.	2015	Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.	26468752
Gould NS et al.	2015	Site-Specific Proteomic Mapping Identifies Selectively Modified Regulatory Cysteine Residues in Functionally Distinct Protein Networks.	26165157
Peng T et al.	2015	Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.	25575299
Pinner AL et al.	2016	Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia.	26876311
Hernandez JL et al.	2016	Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.	27030425
Fang C et al.	2016	Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody.	26865113
Caballero MC et al.	2016	Identification of new palmitoylated proteins in Toxoplasma gondii.	26825284
Kaufman JF et al.	1984	Cysteines in the transmembrane region of major histocompatibility complex antigens are fatty acylated via thioester bonds.	6373770
Upadhyay S et al.	2016	Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation.	27879337
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
Sobocińska J et al.	2018	Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.	29217618
Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Thinon E et al.	2016	Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.	27350074
Thinon E et al.	2018	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.	29575903
Zhang X et al.	2018	Ultradeep Palmitoylomics Enabled by Dithiodipyridine-Functionalized Magnetic Nanoparticles.	29660268
Won SJ et al.	2018	Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.	29733200
Dumoulin A et al.	2018	S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.	30319353
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Wang J et al.	2019	DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane.	30605677
Strassburger K et al.	2019	Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.	30735487
Zhou B et al.	2019	Low-background Acyl-biotinyl Exchange Largely Eliminates the Co-isolation of Non-S-acylated Proteins and Enables Deep S-acylproteomic Analysis.	31251020
Zaręba-Kozioł M et al.	2019	Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins.	31311849
Wegleiter T et al.	2019	Palmitoylation of BMPR1a regulates neural stem cell fate.	31772009
Morrison E et al.	2020	Dynamic palmitoylation events following T-cell receptor signaling.	32651440
Mariscal J et al.	2020	Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles.	32944167
Gorenberg EL et al.	2022	Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.	35358180
Uzbekova S et al.	2021	Protein Palmitoylation in Bovine Ovarian Follicle.	34769186
Miles MR et al.	2021	Global identification of S-palmitoylated proteins and detection of palmitoylating (DHHC) enzymes in heart.	33636221
Pradhan AJ et al.	2021	Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.	33848465
Ripamonti M et al.	2021	Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions.	33782527
Zaręba-Kozioł M et al.	2021	S-Palmitoylation of Synaptic Proteins as a Novel Mechanism Underlying Sex-Dependent Differences in Neuronal Plasticity.	34200797
Ziemlińska E et al.	2021	Palm Oil-Rich Diet Affects Murine Liver Proteome and <i>S</i>-Palmitoylome.	34884899
Gao J et al.	2022	Proteome-wide identification of palmitoylated proteins in mouse testis.	35477839

P01889 HLAB_HUMAN