SwissPalm

P04406 G3P_HUMAN

General info Cross-references Sequences Orthologs Ortholog sequences Validation experiments Sites Predictions Palmitoyl-proteomes References

Palmitoyl-proteomes

11/22 articles for human
6 orthologs

Palmitoyl-proteome experiments

Validation experiments

2 experiments
1 ortholog

Validation experiments

Curated sites & Enzymes

0 site from validation experiments^*
3 sites from palmitoyl-proteomes^* Cys¹⁵², Cys¹⁵⁶, Cys²⁴⁷
No PAT known
No APT known
^*In main isoform only

Sites

Predictions

Not predicted

Predictions

Protein general information from UniProt

Protein names

Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, EC 1.2.1.12; Peptidyl-cysteine S-nitrosylase GAPDH, EC 2.6.99.-

Subcellular location

Cytoskeleton (Probable); Perinuclear region (Probable); Cytosol (Probable); cytoskeleton; Membrane (Probable); perinuclear region; Nucleus (Probable); cytosol; Cytoplasm (Probable)

Isoforms & protein length

Isoform 1 (main): 335 aa; Isoform 2 : 293 aa

Known functions

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = 3-phospho-D-glyceroyl phosphate + H(+) + NADH Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules. Associates with microtubules (By similarity). Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with phosphorylated RPL13A; inhibited by oxidatively-modified low-densitity lipoprotein (LDL(ox)). Component of the GAIT complex. Interacts with FKBP6; leading to inhibit GAPDH catalytic activity. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADH L-cysteinyl-[protein] + S-nitroso-L-cysteinyl-[GAPDH] = L-cysteinyl-[GAPDH] + S-nitroso-L-cysteinyl-[protein] Glyceraldehyde-3-phosphate dehydrogenase activity is inhibited by fumarate, via the formation of S-(2-succinyl)cysteine residues. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Homotetramer (PubMed:16239728, PubMed:16510976). Interacts with TPPP; the interaction is direct (By similarity). Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation (By similarity). Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation (By similarity). Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules (By similarity). Associates with microtubules (By similarity). Interacts with EIF1AD, USP25, PRKCI and WARS1 (PubMed:11724794, PubMed:16501887, PubMed:15628863, PubMed:20644585). Interacts with phosphorylated RPL13A; inhibited by oxidatively-modified low-densitity lipoprotein (LDL(ox)) (PubMed:22771119). Component of the GAIT complex (PubMed:15479637). Interacts with FKBP6; leading to inhibit GAPDH catalytic activity (PubMed:19001379). Interacts with TRAF2, promoting TRAF2 ubiquitination (PubMed:23332158). Interacts with TRAF3, promoting TRAF3 ubiquitination (PubMed:27387501). Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261). Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules. Associates with microtubules (By similarity). Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with phosphorylated RPL13A; inhibited by oxidatively-modified low-densitity lipoprotein (LDL(ox)). Component of the GAIT complex. Interacts with FKBP6; leading to inhibit GAPDH catalytic activity. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Cross-references

Source	Identifiers
UniProt gene names	OK/SW-cl.12, CDABP0047, GAPD, GAPDH
UniProt secondary AC	Q53X65, P00354, E7EUT4
Refseq protein ID	NP_002037.2, NP_001243728.1, NP_001276675.1, NP_001276674.1
IPI ID	IPI00024093, IPI00383758, IPI00797221, IPI00219018

Source	Identifiers
Ensembl protein	ENSP00000380070, ENSP00000380068, ENSP00000380067, ENSP00000229239, ENSP00000478864
Ensembl gene	ENSG00000111640

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

Select all Deselect all

UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Sites	Predicted
Q8IKK7	Q8IKK7_PLAF7	OMA, InParanoid	P. falciparum	PF3D7_1462800	Glyceraldehyde-3-phosphate dehydrogenase	1/1	0	1	0	No
Q9SAJ6	G3PP1_ARATH	MetaPhors, OMA, InParanoid	A. thaliana	T8K14.5, At1g79530, GAPCP1	Glyceraldehyde-3-phosphate dehydrogenase GAPCP1, chloroplastic, EC 1.2.1.12	1/1	0	1	0	No
Q64467	G3PT_MOUSE	OrthoDB	M. musculus	Gapds, Gapd-s, Gapdhs	Glyceraldehyde-3-phosphate dehydrogenase, testis-specific, EC 1.2.1.12	3/23	0	2	0	Yes
Q9ESV6	G3PT_RAT	OrthoDB	R. norvegicus	Gapds, Gapd-s, Gapdhs	Glyceraldehyde-3-phosphate dehydrogenase, testis-specific, EC 1.2.1.12	1/3	0	1	0	Yes
P16858	G3P_MOUSE	MetaPhors, OrthoDB, Ensembl, InParanoid	M. musculus	Gapd, Gapdh	Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, EC 1.2.1.12	10/23	0	3	4	No
O43026	G3P2_SCHPO	MetaPhors, InParanoid	S. pombe	SPBC354.12, gpd3	Glyceraldehyde-3-phosphate dehydrogenase 2	0/1	0	0	0	No
Q7JY07	Q7JY07_DROME	MetaPhors	D. melanogaster	Dmel\CG9010, anon-WO0140519.132, Dmel_CG9010, CG9010	Glyceraldehyde-3-phosphate dehydrogenase	0/1	0	0	0	Yes
P25858	G3PC1_ARATH	MetaPhors	A. thaliana	T6K12.26, At3g04120, GAPDH, GAPC, GAPC1	Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic, EC 1.2.1.12	0/1	0	0	0	No
Q9FX54	G3PC2_ARATH	MetaPhors	A. thaliana	T6J4.17, At1g13440, GAPDH, GAPC2	Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic, EC 1.2.1.12	0/1	0	0	0	No
E9PTV9	E9PTV9_RAT	MetaPhors, InParanoid	R. norvegicus	AABR07032392.1	Glyceraldehyde-3-phosphate dehydrogenase	0/3	0	0	0	No
M0R590	M0R590_RAT	MetaPhors, InParanoid	R. norvegicus	LOC108351137	Glyceraldehyde-3-phosphate dehydrogenase	0/3	0	0	0	No
Q28259	G3P_CANLF	InParanoid	C. familiaris	GAPD, GAPDH	Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, EC 1.2.1.12	0/1	0	0	0	No
V9GX06	V9GX06_MOUSE	MetaPhors	M. musculus	Gm11214	Predicted gene 11214	0/23	0	0	0	No
V9GXK0	V9GXK0_MOUSE	MetaPhors	M. musculus	Gm12117	Predicted gene 12117	0/23	0	0	0	Yes
V9GXA7	V9GXA7_MOUSE	MetaPhors	M. musculus	Gm15294	Predicted gene 15294	0/23	0	0	0	Yes
S4R1W1	S4R1W1_MOUSE	MetaPhors	M. musculus	Gm3839	Glyceraldehyde-3-phosphate dehydrogenase	1/23	0	1	1	No
P78780	DHAS_SCHPO	MetaPhors	S. pombe	SPCC1827.06c	Probable aspartate-semialdehyde dehydrogenase	0/1	0	0	0	No
A0A178W3P2	A0A178W3P2_ARATH	MetaPhors	A. thaliana	C24_LOCUS1525, AN1_LOCUS1608, AXX17_At1g15490	Aspartate-semialdehyde dehydrogenase	0/1	0	0	0	No
P00360	G3P1_YEAST	InParanoid	S. cerevisiae S288c	J1154, YJL052W, SSS2, GPD1, TDH1	Glyceraldehyde-3-phosphate dehydrogenase 1, GAPDH 1, EC 1.2.1.12	0/1	0	0	0	No
P00358	G3P2_YEAST	InParanoid	S. cerevisiae S288c	J1433, YJR009C, GPD2, TDH2	Glyceraldehyde-3-phosphate dehydrogenase 2, GAPDH 2, EC 1.2.1.12	0/1	0	0	0	No
P00359	G3P3_YEAST	InParanoid	S. cerevisiae S288c	G7576, YGR192C, GPD3, TDH3	Glyceraldehyde-3-phosphate dehydrogenase 3, GAPDH 3, EC 1.2.1.12	0/1	0	0	0	No
P46406	G3P_RABIT	InParanoid	O. cuniculus	GAPD, GAPDH	Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, EC 1.2.1.12	0/0	1	0	1	No
E9PTN6	E9PTN6_RAT	InParanoid	R. norvegicus	LOC108352348	Glyceraldehyde-3-phosphate dehydrogenase	0/3	0	0	0	No
D3ZGY4	D3ZGY4_RAT	InParanoid	R. norvegicus	Gapdh-ps2	Glyceraldehyde-3-phosphate dehydrogenase	0/3	0	0	0	No
F1PYE8	F1PYE8_CANLF	OrthoDB	C. familiaris		Glyceraldehyde-3-phosphate dehydrogenase	0/1	0	0	0	No
D3Z0Z9	D3Z0Z9_MOUSE	OrthoDB	M. musculus	Gm5069	Glyceraldehyde-3-phosphate dehydrogenase	0/23	0	0	0	Yes
P04797	G3P_RAT	MetaPhors, OrthoDB, OMA, InParanoid	R. norvegicus	Gapd, Gapdh	Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, EC 1.2.1.12	0/3	0	0	0	No
D3YYI5	D3YYI5_MOUSE	MetaPhors, OrthoDB, Ensembl	M. musculus	Gm7293	Glyceraldehyde-3-phosphate dehydrogenase	0/23	0	0	0	Yes
F1P7C9	F1P7C9_CANLF	OrthoDB, OMA, InParanoid	C. familiaris	LOC106559694	Glyceraldehyde-3-phosphate dehydrogenase	0/1	0	0	0	No
Q2KJE5	G3PT_BOVIN	OrthoDB	B. taurus	GAPDHS	Glyceraldehyde-3-phosphate dehydrogenase, testis-specific, EC 1.2.1.12	0/2	0	0	0	Yes
P07487	G3P2_DROME	MetaPhors, OrthoDB, Ensembl, InParanoid	D. melanogaster	CG8893, Gapdh2	Glyceraldehyde-3-phosphate dehydrogenase 2, EC 1.2.1.12	0/1	0	0	0	No
Q5E924	G3PP2_ARATH	MetaPhors, OMA, InParanoid	A. thaliana	F3O9.10, At1g16300, GAPCP2	Glyceraldehyde-3-phosphate dehydrogenase GAPCP2, chloroplastic, EC 1.2.1.12	0/1	0	0	0	No
P78958	G3P1_SCHPO	MetaPhors, OMA, InParanoid	S. pombe	SPBC32F12.11, gpd1, tdh1	Glyceraldehyde-3-phosphate dehydrogenase 1	0/1	0	0	0	No
D2KHZ9	D2KHZ9_MOUSE	OMA	M. musculus	Gapdh, GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	0/23	0	0	0	No
F1PTZ9	F1PTZ9_CANLF	OMA	C. familiaris	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	0/1	0	0	0	No
L7N0H7	L7N0H7_CANLF	OMA	C. familiaris	LOC100683724	Glyceraldehyde-3-phosphate dehydrogenase	0/1	0	0	0	No
P10096	G3P_BOVIN	MetaPhors, OMA, OrthoDB, InParanoid	B. taurus	GAPD, GAPDH	Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, EC 1.2.1.12	0/2	0	0	0	No
P07486	G3P1_DROME	MetaPhors, OMA, OrthoDB, Ensembl, InParanoid	D. melanogaster	CG12055, Gadph-1, Gapdh1	Glyceraldehyde-3-phosphate dehydrogenase 1, EC 1.2.1.12	0/1	0	0	0	No
M0R660	M0R660_RAT	MetaPhors, OrthoDB, InParanoid	R. norvegicus	LOC108351683	Glyceraldehyde-3-phosphate dehydrogenase	0/3	0	0	0	No

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:1342	Chen JJ et al.	2020	Rattus norvegicus	NA	NA	[3H]palmitoyl-CoA labeling
SPalmE:1411	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	LB-ABE, MS

Sites (from validation or large-scale experiments)

Isoforms	Position	Probability	Studies	Techniques
P04406	247	NA	SPalmE:724 Fang C et al. (2016)*	ARAC
P04406	152	1.0	SPalmE:1185 Thinon E et al. (2018) []*	Alk16 Labeling, LC-MS, NH2OH cleavage of thioester bond
P04406	156	1.0	SPalmE:1185 Thinon E et al. (2018) []*	Alk16 Labeling, LC-MS, NH2OH cleavage of thioester bond
P04406	247	1.0	SPalmE:1185 Thinon E et al. (2018) []*	Alk16 Labeling, LC-MS, NH2OH cleavage of thioester bond

Cysteine summary and predictions

Pos in alignment	152	156	247
Subcell. location	C	C	C
Disulfide	no	no	no
PSIPRED v.4.02 prediction	coil	coil	strand
UniProt secondary structure	helix	helix	strand
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys¹⁵²	Cys¹⁵⁶	Cys²⁴⁷ LC
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys¹¹⁰	Cys¹¹⁴	Cys²⁰⁵ LC

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

Tip: Keep CTRL key pressed down while clicking on column headers to do multiple sorting. This applies to any sortable table on this site.

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	Ortholog	in LC [G3PT]	HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	x		HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	x		HC: 102 hits LC: 73 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	x		HC: 162 hits LC: 218 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	Found	in List	List: 69 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	x		HC HC: 352 hits LC LC: 8 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	Found	in HC	HC: 164 hits LC: 178 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	Ortholog	in LC [Q8IKK7]	HC: 79 hits LC: 89 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	Ortholog	in List [G3P, G3PT, S4R1W1]	List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	Ortholog	in List [G3P, G3PT, S4R1W1]	List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	Ortholog	in LC [G3PP1]	HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	x		Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	Ortholog	in List [G3P, G3PT, S4R1W1]	List: 436 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Found	in List	List: 1348 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	x		List: 274 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	x		List: 226 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	Found	in List	List: 113 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	Ortholog	in List [G3P, G3PT, S4R1W1]	List: 780 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	x		List: 354 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	x		List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	Found	in List	List: 1353 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	Found	in List	List: 100 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	Ortholog	in HC [G3P, G3PT, S4R1W1]	HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	Ortholog	in List [G3P, G3PT, S4R1W1]	List: 865 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	Ortholog	in HC [G3P, G3PT, S4R1W1]	HC: 1079 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		List: 646 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		LPS treatment: 646 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in MC [G3P, G3PT, S4R1W1]	HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	Found	in Trypsin	Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in MC [G3P, G3PT, S4R1W1]	HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Ortholog	in HC [G3P, G3PT, S4R1W1]	HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	Found	in List	List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	Found	in List	List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	Found	in List	List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 590 hits
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	Found	in List	List: 2929 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 370 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		List: 185 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1869 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 600 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [G3P, G3PT, S4R1W1]	HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Found	in List	List: 687 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 399 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 464 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [G3P, G3PT, S4R1W1]	List: 199 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [G3P, G3PT, S4R1W1]	List: 1093 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [G3P, G3PT, S4R1W1]	List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [G3P, G3PT, S4R1W1]	List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [G3P, G3PT, S4R1W1]	List: 1040 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [G3P, G3PT, S4R1W1]	HC: 4107 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [G3P, G3PT, S4R1W1]	HC: 3870 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [G3P, G3PT, S4R1W1]	List: 1378 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [G3P, G3PT, S4R1W1]	List: 1597 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	1	25.0	G3PT_RAT
H. sapiens	22	38	16	42.1	E7EUT5_HUMAN
M. musculus	23	37	20	54.1	G3P_MOUSE , G3PT_MOUSE , S4R1W1_MOUSE
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	1	50.0	Q8IKK7_PLAF7
S. pombe	1	1	0	0.0	NA
A. thaliana	1	1	1	100.0	G3PP1_ARATH
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	0	0.0	NA
C. familiaris	1	1	0	0.0	NA

References

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Gao J et al.	2022	Proteome-wide identification of palmitoylated proteins in mouse testis.	35477839
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Pradhan AJ et al.	2021	Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.	33848465
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Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
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