SwissPalm

Protein general information from UniProt

Protein names

Tyrosine-protein kinase Lck; EC 2.7.10.2; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK

Subcellular location

Cytoplasm (Probable); Cell membrane (By similarity)

Isoforms & protein length

Isoform 1 (main): 509 aa; Isoform 2 : 363 aa; Isoform 3 : 539 aa

Known functions

Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with FYB2 (PubMed:27335501). ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein] The relative activities of the inhibitory tyrosine-protein kinase CSK and the activating tyrosine-protein phosphatase PTPRC/CD45 determine the level of LCK activity. These interactions allow rapid and efficient activation of LCK in response to TCR stimulation. Binds to the cytoplasmic domain of cell surface receptors, such as AXL, CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB1 and to other protein kinases including CDK1, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with SQSTM1. Interacts with phosphorylated LIME1. Interacts with CBLB and PTPRH. Interacts with RUNX3. Forms a signaling complex with EPHA1, PTK2B AND PI3-KINASE; upon activation by EFNA1 which may regulate T-lymphocyte migration. Associates with ZAP70 and RHOH; these interactions allow LCK-mediated RHOH and CD3 subunit phosphorylation in the presence of functional ZAP70. Interacts with UNC119; this interaction plays a crucial role in activation of LCK. Interacts with CEACAM1 (via cytoplasmic domain); mediates CEACAM1 phosphorylation resulting in PTPN6 recruitment that dephosphorylates TCR stimulation-induced CD247 and ZAP70 (PubMed:18424730). Interacts with CD160. (Microbial infection) Interacts with herpes simplex virus 1 UL46; this interaction activates LCK. (Microbial infection) Interacts with HIV-1 Nef through its SH3 domain. Present in lipid rafts in an inactive form. Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with FYB2 (PubMed:27335501). ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The relative activities of the inhibitory tyrosine-protein kinase CSK and the activating tyrosine-protein phosphatase PTPRC/CD45 determine the level of LCK activity. These interactions allow rapid and efficient activation of LCK in response to TCR stimulation. Binds to the cytoplasmic domain of cell surface receptors, such as AXL, CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB1 and to other protein kinases including CDK1, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with herpes simplex virus 1 UL46; this interaction activates LCK. Interacts with SQSTM1. Interacts with phosphorylated LIME1. Interacts with CBLB and PTPRH. Interacts with RUNX3. Forms a signaling complex with EPHA1, PTK2B AND PI3-KINASE; upon activation by EFNA1 which may regulate T-lymphocyte migration. Associates with ZAP70 and RHOH; these interactions allow LCK-mediated RHOH and CD3 subunit phosphorylation in the presence of functional ZAP70. Interacts with UNC119; this interaction plays a crucial role in activation of LCK. Interacts with CEACAM1 (via cytoplasmic domain); mediates CEACAM1 phosphorylation resulting in PTPN6 recruitment that dephosphorylates TCR stimulation-induced CD247 and ZAP70 (PubMed:18424730). Present in lipid rafts in an inactive form.

Cross-references

Source	Identifiers
UniProt gene names	LCK
UniProt secondary AC	Q9NYT8, Q96DW4, Q7RTZ3, Q5TDH9, Q5TDH8, Q13152, Q12850, P07100, D3DPP8
Refseq protein ID	XP_005270922.1, NP_005347.3, NP_001036236.1
IPI ID	IPI00479530, IPI00940423, IPI00515097, IPI00411413

Source	Identifiers
Ensembl protein	ENSP00000337825, ENSP00000328213, ENSP00000477713
Ensembl gene	ENSG00000182866

Enzymes

PAT/APT	Enzyme	Experiments
PAT	ZDHC2_HUMAN	SPalmE:1088 Zeidman R et al. (2011) SPalmE:305 Zeidman R et al. (2011)
PAT	ZDH21_HUMAN	SPalmE:147 Mill P et al. (2009) SPalmE:545 Akimzhanov AM et al. (2015)
PAT	ZDH21_MOUSE	SPalmE:112 Tsutsumi R et al. (2009)
APT	LYPA1_HUMAN	SPalmE:545 Akimzhanov AM et al. (2015)

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

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UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Sites	Predicted
Q9FGB1	Q9FGB1_ARATH	InParanoid	A. thaliana	At5g50000, MPF21_1, MPF21.1	Protein kinase	1/1	0	1	0	Yes
Q9SSA4	Q9SSA4_ARATH	InParanoid	A. thaliana	F4P13_4, F4P13.4, At3g01490	AT3g01490/F4P13_4	0/1	0	0	0	Yes
Q8L6Y9	Q8L6Y9_ARATH	InParanoid	A. thaliana	T20L15_120, T20L15.120, At5g01850	Protein kinase ATN1-like protein	0/1	0	0	0	No
O23334	O23334_ARATH	InParanoid	A. thaliana	FCAALL.308, Dl3430W, At4g14780/dl3430w, At4g14780, dl3430w	Kinase like protein	0/1	0	0	0	Yes
Q9FM43	Q9FM43_ARATH	InParanoid	A. thaliana	At5g40540, MNF13_60, MNF13.60	Protein kinase ATN1	0/1	0	0	0	Yes
Q9FGS7	Q9FGS7_ARATH	InParanoid	A. thaliana	K6A12_4, K6A12.4, At5g50180	At5g50180	0/1	0	0	0	Yes
Q9LUI6	Q9LUI6_ARATH	InParanoid	A. thaliana	At3g22750	Kinase-like protein	0/1	0	0	0	Yes
Q9LT56	Q9LT56_ARATH	InParanoid	A. thaliana	At3g27560, ATN1	AT3g27560/MMJ24_11	0/1	0	0	0	Yes
O22100	O22100_ARATH	InParanoid	A. thaliana	At3g63260, ATMRK1, F16M2_110	ATMRK1	1/1	0	1	0	Yes
Q2MHE4	HT1_ARATH	InParanoid	A. thaliana	SUU, F24O1.13, At1g62400, HT1	Serine/threonine/tyrosine-protein kinase HT1	0/1	0	0	0	Yes
Q8RWL6	STY17_ARATH	InParanoid	A. thaliana	F8D20.290, At4g35780, STY17	Serine/threonine-protein kinase STY17	0/1	0	0	0	No
Q9M085	Q9M085_ARATH	InParanoid	A. thaliana	F6E21_90, F6E21.90, At4g31170	AT4G31170 protein	0/1	0	0	0	Yes
Q9ZQ31	STY13_ARATH	InParanoid	A. thaliana	T28I24.9, At2g24360, STY13	Serine/threonine-protein kinase STY13	0/1	0	0	0	Yes
F4JTP5	STY46_ARATH	InParanoid	A. thaliana	F20M13.30, At4g38470, STY46	Serine/threonine-protein kinase STY46	0/1	0	0	0	No
O22558	STY8_ARATH	InParanoid	A. thaliana	At2g17700, STY8	Serine/threonine-protein kinase STY8	0/1	0	0	0	Yes
Q07014	LYN_RAT	OrthoDB	R. norvegicus	Lyn	Tyrosine-protein kinase Lyn	0/2	0	0	0	Yes
Q8IIT5	Q8IIT5_PLAF7	InParanoid	P. falciparum	PF3D7_1106800	Protein kinase, putative	0/1	0	0	0	Yes
P00522	ABL_DROME	OrthoDB	D. melanogaster	CG4032, Dash, ABL-1, Abl	Tyrosine-protein kinase Abl	0/1	0	0	0	No
P06240	LCK_MOUSE	OrthoDB, Ensembl, InParanoid	M. musculus	Lsk-t, Lck	Proto-oncogene tyrosine-protein kinase LCK	1/17	4	1	4	Yes
Q01621	LCK_RAT	OMA, OrthoDB, InParanoid	R. norvegicus	Lck	Proto-oncogene tyrosine-protein kinase LCK	0/2	0	0	0	Yes
F1MWN2	F1MWN2_BOVIN	OrthoDB	B. taurus	HCK	Tyrosine-protein kinase	0/1	0	0	0	Yes
P00528	SRC64_DROME	OrthoDB, Ensembl	D. melanogaster	CG7524, Src1, Src64B	Tyrosine-protein kinase Src64B	0/1	0	0	0	Yes
E9Q696	E9Q696_MOUSE	OMA, Ensembl	M. musculus	Lck	Tyrosine-protein kinase	0/17	0	0	0	Yes
Q3ZCM0	Q3ZCM0_BOVIN	OMA, Ensembl, InParanoid	B. taurus	LCK	Tyrosine-protein kinase	0/1	0	0	0	Yes

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:112	Tsutsumi R et al.	2009	Homo sapiens	HEK293T	Total cell lysate	PAT overexpression, [3H]palmitic acid labeling
SPalmE:147	Mill P et al.	2009	Homo sapiens	HEK293	NA	PAT localization by IF, PAT overexpression, PAT point mutation, [3H]palmitic acid labeling
SPalmE:305	Zeidman R et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Depletion of PAT by siRNA, [3H]palmitic acid labeling
SPalmE:525	Rodgers W et al.	1994	Homo sapiens	HeLa	Total cell lysate	Point mutation, [3H]palmitic acid labeling
SPalmE:545	Akimzhanov AM et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, Depletion of PAT by shRNA, Thioesterases drug inhibition
SPalmE:779	Zlatkine P et al.	1997	Homo sapiens	COS7	Total cell lysate	Point mutation, Substrate localization by fluorescence tag, [3H]palmitic acid labeling
SPalmE:1060	Charron G et al.	2009	Homo sapiens	Jurkat	Total cell lysate	, Alk12 labeling, Alk16 Labeling, Click-IT Palmitic Acid labeling, NH2OH cleavage of thioester bond
SPalmE:1088	Zeidman R et al.	2011	Homo sapiens	HEK293T	Total cell lysate	PAT overexpression, [3H]palmitic acid labeling

Sites (from validation or large-scale experiments)

Isoforms	Position	Probability	Studies	Techniques
P06239	5	NA	SPalmE:525 Rodgers W et al. (1994)	Point mutation, [3H]palmitic acid labeling

Cysteine summary and predictions

Pos in alignment	3	5	20	23	217	224	408	495	506
Subcell. location	C	C	C	C	C	C	C	C	C
Disulfide	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	coil	strand	helix	coil	coil	coil
UniProt secondary structure	NA	NA	NA	strand	strand	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys³ HC HC	Cys⁵ HC HC	Cys²⁰ HC	Cys²³	Cys²¹⁷	Cys²²⁴	Cys³⁷⁸ HC	Cys⁴⁶⁵	Cys⁴⁷⁶
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys³ HC HC	Cys⁵ HC HC	Cys²⁰ HC	Cys²³	Cys²¹⁷	Cys²²⁴
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys³ HC HC	Cys⁵ HC HC	Cys²⁰ HC	Cys²³	Cys²¹⁷	Cys²²⁴	Cys⁴⁰⁸ HC	Cys⁴⁹⁵	Cys⁵⁰⁶

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

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ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	Found	in HC	HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	x		HC: 102 hits LC: 73 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	x		HC: 162 hits LC: 218 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	Found	in HC	HC: 164 hits LC: 178 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	Ortholog	in HC [LCK]	HC HC: 352 hits LC LC: 8 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	Found	in HC	HC HC: 93 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	Found	in List	List: 154 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	Ortholog	in HC,LC [O22100, Q9FGB1]	HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	x		Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	x		List: 436 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	x		List: 1348 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	Found	in List	List: 274 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	Found	in List	List: 226 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	x		List: 354 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	x		List: 354 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	x		List: 1353 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	x		HC: 1079 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		List: 865 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		List: 646 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		LPS treatment: 646 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	x		List: 1404 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	x		List: 1394 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	2	3	0	0.0	LYN_RAT
H. sapiens	17	26	6	23.1	NA
M. musculus	17	25	1	4.0	LCK_MOUSE
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
A. thaliana	1	1	1	100.0	O22100_ARATH , Q9FGB1_ARATH
S. pombe	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	1	1	0	0.0	NA
D. melanogaster	1	2	0	0.0	SRC64_DROME

References

Authors	Year	Reference title	PMID
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Colquhoun DR et al.	2015	Bioorthogonal mimetics of palmitoyl-CoA and myristoyl-CoA and their subsequent isolation by click chemistry and characterization by mass spectrometry reveal novel acylated host-proteins modified by HIV-1 infection.	25914232
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
Thinon E et al.	2016	Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.	27350074
Kang R et al.	2008	Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.	19092927
Wang J et al.	2019	DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane.	30605677
Yang W et al.	2010	Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes.	19801377
Upadhyay S et al.	2016	Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation.	27879337
Won SJ et al.	2018	Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.	29733200
Pinner AL et al.	2016	Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia.	26876311
Dowal L et al.	2011	Proteomic analysis of palmitoylated platelet proteins.	21813449
Rodgers W et al.	1994	Signals determining protein tyrosine kinase and glycosyl-phosphatidylinositol-anchored protein targeting to a glycolipid-enriched membrane fraction.	8035816
Martin BR et al.	2009	Large-scale profiling of protein palmitoylation in mammalian cells.	19137006
Chesarino NM et al.	2014	Chemoproteomics reveals Toll-like receptor fatty acylation.	25371237
Ivaldi C et al.	2012	Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.	22615937
Caballero MC et al.	2016	Identification of new palmitoylated proteins in Toxoplasma gondii.	26825284
Wei X et al.	2014	Insulin-regulated protein palmitoylation impacts endothelial cell function.	24357059
Dumoulin A et al.	2018	S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.	30319353
Forrester MT et al.	2011	Site-specific analysis of protein S-acylation by resin-assisted capture.	21044946
Zhang MM et al.	2013	Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.	23843742
Zhang X et al.	2018	Ultradeep Palmitoylomics Enabled by Dithiodipyridine-Functionalized Magnetic Nanoparticles.	29660268
Peng T et al.	2015	Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.	25575299
Wan J et al.	2013	Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease.	24211138
Zeidman R et al.	2011	DHHC2 is a protein S-acyltransferase for Lck.	22034844
Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
Sobocińska J et al.	2018	Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.	29217618
Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
Santiago-Tirado FH et al.	2015	A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.	25970403
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
Mill P et al.	2009	Palmitoylation regulates epidermal homeostasis and hair follicle differentiation.	19956733
Strassburger K et al.	2019	Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.	30735487
Charron G et al.	2009	Robust fluorescent detection of protein fatty-acylation with chemical reporters.	19281244
Martin BR et al.	2011	Global profiling of dynamic protein palmitoylation.	22056678
Jones ML et al.	2012	Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis.	22901544
Foe IT et al.	2015	Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.	26468752
Gould NS et al.	2015	Site-Specific Proteomic Mapping Identifies Selectively Modified Regulatory Cysteine Residues in Functionally Distinct Protein Networks.	26165157
Serwa RA et al.	2015	Systems Analysis of Protein Fatty Acylation in Herpes Simplex Virus-Infected Cells Using Chemical Proteomics.	26256475
Hernandez JL et al.	2016	Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.	27030425
Zaręba-Kozioł M et al.	2019	Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins.	31311849
Thinon E et al.	2018	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.	29575903
Tsutsumi R et al.	2009	Identification of G protein alpha subunit-palmitoylating enzyme.	19001095
Zlatkine P et al.	1997	Retargeting of cytosolic proteins to the plasma membrane by the Lck protein tyrosine kinase dual acylation motif.	9092949
Morrison E et al.	2015	Quantitative analysis of the human T cell palmitome.	26111759
Marin EP et al.	2012	Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases.	22496122
Merrick BA et al.	2011	Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3.	21785166
Emmer BT et al.	2011	Global analysis of protein palmitoylation in African trypanosomes.	21193548
Ren W et al.	2013	Proteomic analysis of protein palmitoylation in adipocytes.	23599907
Fang C et al.	2016	Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody.	26865113
Akimzhanov AM et al.	2015	Rapid and transient palmitoylation of the tyrosine kinase Lck mediates Fas signaling.	26351666
Roth AF et al.	2006	Global analysis of protein palmitoylation in yeast.	16751107
Hemsley PA et al.	2013	A proteomic approach identifies many novel palmitoylated proteins in Arabidopsis.	23252521

P06239 LCK_HUMAN