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P62344 CDPK1_PLAF7

General info Cross-references Sequences Orthologs Ortholog sequences Validation experiments Predictions Palmitoyl-proteomes References

Palmitoyl-proteomes

1/1 article for plasmodium falciparum
15 orthologs

Palmitoyl-proteome experiments

Validation experiments

1 experiment
2 orthologs

Validation experiments

Curated sites & Enzymes

0 site from validation experiments^*
0 site from palmitoyl-proteomes^*
No PAT known
No APT known
^*In main isoform only

Predictions

Predicted

1 cysteine in main isoform

Predictions

Protein general information from UniProt

Protein names

Calcium-dependent protein kinase 1, EC 2.7.11.1; PfCDPK1

Subcellular location

Membrane (Potential); Membrane; Cell membrane; Parasitophorous vacuole membrane; Cytoplasm (Potential); Cell projection (Potential); Cilium (Potential); Flagellum (Potential); Host cell membrane

Isoforms & protein length

Isoform 1 (main): 524 aa

Known functions

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily. Calcium-dependent protein kinase which acts as a sensor and effector of intracellular Ca(2+) levels probably in part downstream of cGMP-activated PKG kinase (PubMed:18768477, PubMed:19307175, PubMed:28680058, PubMed:29716996). By phosphorylating various proteins, required for microneme secretion and thus merozoite egress from and invasion of host erythrocytes (PubMed:23204525, PubMed:28680058, PubMed:29716996). During gametogenesis, essential for the development of both male and female gametes (By similarity). Phosphorylates SERA5 p50 which enhances SERA5 p50 protease activity; however, SERA5 p50 protease activity has been shown in other studies to be controversial (PubMed:29716996). Probably by phosphorylating SERA5 p50, plays a role in merozoite egress from host erythrocytes (PubMed:29716996). Probably prior or during merozoite invasion of host erythrocytes, phosphorylates rhoptry protein RhopH3 which is required for RhopH3 localization to rhoptries and for its secretion (PubMed:33024030). Probably in late schizonts, phosphorylates myosin A tail domain-interacting protein MTIP and glideosome-associated protein 45 GAP45, both of which are components of the motor complex that generates the force required by the parasite to invade host cells (PubMed:18768477, PubMed:28680058, PubMed:22539638). In late schizonts, phosphorylates inner membrane complex protein IMC1g (PubMed:28680058). In late schizonts, phosphorylates PKA regulatory subunit PKAr in a calcium-dependent manner, which may contribute to the dissociation of regulatory PKAr and catalytic PKAc subunits and promote the activation of PKAc (PubMed:28680058). May phosphorylate raf kinase inhibitory protein RKIP which in turn may regulate CDPK1 catalytic activity (PubMed:17123645). May phosphorylate proteins of the host erythrocyte membranes (By similarity). ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein] ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein] Activated by calcium (PubMed:29716996, PubMed:18768477, PubMed:23204525, PubMed:17123645, PubMed:32484216). Upon calcium binding to the EF-hand domains, the C-terminus of the junction domain (J domain) undergoes a conformational change which results in the dissociation of the pseudo-substrate inhibitory motif from the catalytic domain (Probable) (PubMed:23204525). This, in turn may facilitate the autophosphorylation of the activation loop at Thr-231, which leads to the kinase activation (PubMed:19307175). May be negatively regulated by PKA-mediated phosphorylation (PubMed:28680058). Inhibited by purfalcamine (PubMed:23204525, PubMed:29716996). Monomer (PubMed:26149123). Forms a high molecular weight (250 and 400 kDa) complex (PubMed:26149123). Forms a complex composed of CDPK1, PKA regulatory subunit PKAr and 14-3-3I; the complex is formed in merozoites in response to low extracellular level of K(+) and may play a role in microneme secretion (PubMed:32817103). Interacts (when phosphorylated) with 14-3-3I in a Ca(2+)-independent manner; the interaction does not regulate CDPK1 catalytic activity but is required for merozoite invasion of host erythrocytes (PubMed:32484216, PubMed:32817103). Interacts with PKA regulatory subunit PKAr; in a Ca(2+)-dependent manner (PubMed:28680058, PubMed:32817103). Interacts with SERA5 p50 in the late schizont stage (PubMed:29716996). Interacts with inner membrane complex protein IMC1g in late schizonts (PubMed:28680058). Interacts with rhoptry protein RhopH3 in merozoites (PubMed:33024030). Localizes to the host erythrocytic membrane at low level (By similarity). Localizes to the cell membrane in the nascent merozoites contained within the late-stage schizonts and in free merozoites (PubMed:26149123, PubMed:18768477). Colocalizes with MTIP around developing merozoites in segmented schizonts, also localizes in membranes around the mature food vacuole/residual body of the schizonts (PubMed:18768477). Ser-64 phosphorylated form localizes at the apical pole in punctate structures in merozoites within late schizonts in free merozoites (PubMed:26149123). In trophozoites and schizonts, localizes to the parasitophorous vacuole (PV) and in membranous systems derived from the PV including intraparasitic vacuoles and the tubovesicular system, an extension of the parasitophorous vacuole membrane into the host cell cytoplasm (PubMed:15491359, PubMed:23204525). Localization to the cytoplasm in trophozoite or schizonts is minimal (PubMed:15491359). In female stage V gametocytes and gametes, localizes to the cell membrane (By similarity). In stage V male gametocytes, localizes to the cell membrane and in the cytoplasm (By similarity). In male gametes, localizes to the residual body, cell membrane and in the flagella (By similarity). Calcium and/or autophosphorylation does not affect membrane localization (PubMed:15491359). Expressed during parasite asexual blood stages, specifically at the ring and schizont stages, in free merozoites and to a lesser extent in trophozoites (at protein level). The junction domain (J domain) is composed of 2 motifs that maintain the kinase inactive (PubMed:19307175). The N-terminal autoinhibitory motif acts as a pseudosubstrate inhibiting the catalytic domain while the C-terminal motif binds the EF-hand domains (PubMed:19307175, PubMed:23204525). Myristoylated (PubMed:15491359). Myristoylation, palmitoylation and the basic cluster motif are required for the localization to the parasitophorous vacuole membrane (PubMed:15491359). Palmitoylated (Probable) (PubMed:32003970). Palmitoylation increases in merozoites in response to low level of extracellular K(+) in the host blood (PubMed:32003970). Myristoylation, palmitoylation and the basic cluster motif are required for the localization to the parasitophorous vacuole membrane (PubMed:15491359). Phosphorylation at Ser-64 occurs at late schizont stage and regulates CDPK1 protein-protein interaction (PubMed:26149123). Phosphorylated at Ser-28, Ser-34 and Ser-64 in merozoites in response to low extracellular level of K(+) (PubMed:32817103). Phosphorylation at Thr-231 may regulate CDPK1 kinase activity (PubMed:26149123). Phosphorylation increases in response to an increase in intracellular Ca(2+) levels (PubMed:32484216). Autophosphorylated in vitro (PubMed:17123645, PubMed:22539638, PubMed:23204525, PubMed:26149123, PubMed:32484216). Autophosphorylation does not affect membrane localization in vitro (PubMed:15491359). Conditional knockdown at the ring stage produces the development of mature schizonts and the subsequent release of merozoites from the host erythrocytes; however, the invasion of new erythrocytes by these merozoites is severely reduced due to a severe defect in adhesion to the erythrocyte membrane (PubMed:28680058). In merozoites, release of host glycophorin A/GYPA ligand EBA-175 from micronemes is impaired but not AMA1 (PubMed:28680058). In late schizont, phosphorylation of several proteins including GAP45, IMC1g and PKAr is reduced (PubMed:28680058). Reduced PKA activity (PubMed:28680058). Rhoptry protein RhopH3 phosphorylation and secretion are reduced in merozoites (PubMed:33024030). Unlike P.berghei CDPK1, appears to be involved in the asexual blood stage and in male gamete exflagellation prior host erythrocyte membrane rupture. Calcium-dependent kinase which may phosphorylate proteins of the host erythrocyte membranes. ATP + a protein = ADP + a phosphoprotein. Activated by calcium. Inhibited by calmodulin antagonists such as calmidazolium, trifluoperazine, N-[6-aminohexyl]-5-chloro-1-naphthalene-sulfonamide, and ophiobolin A (By similarity). In the parasite and on erythrocytic membrane at a lower level. May be myristoylated; myristoylation may target it to different subcellular compartments. Autophosphorylated in vitro. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily. Calcium-dependent kinase which may phosphorylate proteins of the host erythrocyte membranes. ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein] ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein] Activated by calcium. Inhibited by calmodulin antagonists such as calmidazolium, trifluoperazine, N-[6-aminohexyl]-5-chloro-1-naphthalene-sulfonamide, and ophiobolin A (By similarity). In the parasite and on erythrocytic membrane at a lower level. May be myristoylated; myristoylation may target it to different subcellular compartments. Autophosphorylated in vitro. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.

Cross-references

Source	Identifiers
UniProt gene names	PF3D7_0217500, CPK1, CDPK1, PFB0815w
UniProt secondary AC	Q27731
Refseq protein ID	XP_001349680.1

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Isoform sequence alignment

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Orthologs

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UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Sites	Predicted
Q6NLQ6	CDPKW_ARATH	OMA	A. thaliana	CPK32, At3g57530, T8H10.130	Calcium-dependent protein kinase 32, EC 2.7.11.1	1/1	0	1	0	Yes
Q42438	CDPK8_ARATH	OMA	A. thaliana	CPK8, CDPK19, At5g19450, F7K24.200	Calcium-dependent protein kinase 8, EC 2.7.11.1	1/1	0	1	0	Yes
Q8W4I7	CDPKD_ARATH	OMA	A. thaliana	CPK13, At3g51850, ATEM1.10	Calcium-dependent protein kinase 13, EC 2.7.11.1	1/1	0	1	0	Yes
Q9ZSA2	CDPKL_ARATH	OMA	A. thaliana	CPK21, At4g04720, T4B21.13	Calcium-dependent protein kinase 21, EC 2.7.11.1	1/1	0	1	0	Yes
Q9S9V0	CDPKV_ARATH	OMA	A. thaliana	CPK31, At4g04695, T19J18.7	Calcium-dependent protein kinase 31, EC 2.7.11.1	1/1	0	1	0	Yes
Q13557	KCC2D_HUMAN	InParanoid	H. sapiens	CAMK2D, CAMKD	Calcium/calmodulin-dependent protein kinase type II subunit delta, CaM kinase II subunit delta, CaMK-II subunit delta, EC 2.7.11.17	3/22	0	3	0	Yes
Q13555	KCC2G_HUMAN	InParanoid	H. sapiens	CAMK2G, CAMK, CAMK-II, CAMKG	Calcium/calmodulin-dependent protein kinase type II subunit gamma, CaM kinase II subunit gamma, CaMK-II subunit gamma, EC 2.7.11.17	2/22	0	2	0	Yes
Q9UQM7	KCC2A_HUMAN	InParanoid	H. sapiens	CAMK2A, CAMKA, KIAA0968	Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, EC 2.7.11.17	0/22	0	0	0	Yes
Q13554	KCC2B_HUMAN	InParanoid	H. sapiens	CAMK2B, CAM2, CAMK2, CAMKB	Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CaMK-II subunit beta, EC 2.7.11.17	3/22	0	3	0	Yes
Q6PHZ2	KCC2D_MOUSE	InParanoid	M. musculus	Camk2d, Kiaa4163	Calcium/calmodulin-dependent protein kinase type II subunit delta, CaM kinase II subunit delta, CaMK-II subunit delta, EC 2.7.11.17	3/23	0	2	0	Yes
Q923T9	KCC2G_MOUSE	InParanoid	M. musculus	Camk2g	Calcium/calmodulin-dependent protein kinase type II subunit gamma, CaM kinase II subunit gamma, CaMK-II subunit gamma, EC 2.7.11.17	3/23	0	2	0	Yes
P28652	KCC2B_MOUSE	InParanoid	M. musculus	Camk2b, Camk2d	Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CaMK-II subunit beta, EC 2.7.11.17	5/23	0	2	1	Yes
P11798	KCC2A_MOUSE	InParanoid	M. musculus	Camk2a	Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, EC 2.7.11.17	4/23	0	2	1	Yes
F1PD00	F1PD00_CANLF	InParanoid	C. familiaris	CAMK1D	Calcium/calmodulin dependent protein kinase ID	0/1	0	0	0	No
E2QWM5	E2QWM5_CANLF	InParanoid	C. familiaris	CAMK1	Calcium/calmodulin dependent protein kinase I	0/1	0	0	0	Yes
E2QUC1	E2QUC1_CANLF	InParanoid	C. familiaris	CAMK1G	Calcium/calmodulin dependent protein kinase IG	0/1	0	0	0	Yes
F1PZJ4	F1PZJ4_CANLF	InParanoid	C. familiaris	PNCK	Pregnancy up-regulated nonubiquitous CaM kinase	0/1	0	0	0	No
E2QWJ1	E2QWJ1_CANLF	InParanoid	C. familiaris	CAMKV	CaM kinase like vesicle associated	0/1	0	0	0	Yes
E2RKP1	E2RKP1_CANLF	InParanoid	C. familiaris	PSKH1	Protein serine kinase H1	0/1	0	0	0	Yes
Q00168	KCC2A_DROME	InParanoid	D. melanogaster	CaMKII, CaM, CG18069	Calcium/calmodulin-dependent protein kinase type II alpha chain, CaM-kinase II alpha chain, EC 2.7.11.17	0/1	0	0	0	Yes
Q2HJF7	KCC2D_BOVIN	InParanoid	B. taurus	CAMK2D	Calcium/calmodulin-dependent protein kinase type II subunit delta, CaM kinase II subunit delta, CaMK-II subunit delta, EC 2.7.11.17	0/2	0	0	0	Yes
Q08E45	Q08E45_BOVIN	InParanoid	B. taurus	CAMK2A	Calcium/calmodulin-dependent protein kinase	0/2	0	0	0	Yes
Q3MHJ9	KCC2B_BOVIN	InParanoid	B. taurus	CAMK2B	Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CaMK-II subunit beta, EC 2.7.11.17	0/2	0	0	0	Yes
F1MVF1	F1MVF1_BOVIN	InParanoid	B. taurus	CAMK2G	Calcium/calmodulin-dependent protein kinase	0/2	0	0	0	Yes
P53683	CDPKJ_ORYSJ	OMA	O. sativa	CPK19, CPK2, Os07g0515100, LOC_Os07g33110, P0048D08.112	Calcium-dependent protein kinase 19, OsCDPK19, OsCPK19, EC 2.7.11.1	0/0	1	0	2	Yes
F4JGW8	F4JGW8_ARATH	OMA	A. thaliana	At4g04740, ATCPK23, T4B21.15, T4B21_15, CPK23	Calcium-dependent protein kinase 23	0/1	0	0	0	Yes
Q38869	CDPK4_ARATH	OMA	A. thaliana	CPK4, At4g09570, T25P22.10	Calcium-dependent protein kinase 4, EC 2.7.11.1	0/1	0	0	0	No
F4JKC7	F4JKC7_ARATH	OMA	A. thaliana	CPK15, At4g21940, F1N20.5	Calcium-dependent protein kinase 15	0/1	0	0	0	Yes
Q42479	CDPK3_ARATH	OMA	A. thaliana	CPK3, CDPK6, At4g23650, F9D16.120	Calcium-dependent protein kinase 3, EC 2.7.11.1	0/1	0	0	0	No
Q38871	CDPK5_ARATH	OMA	A. thaliana	CPK5, At4g35310, F23E12.130	Calcium-dependent protein kinase 5, EC 2.7.11.1	0/1	0	0	0	Yes
F4JTL3	F4JTL3_ARATH	OMA	A. thaliana	At4g38230, ATCPK26, F20D10.350, F20D10_350, CPK26	Calcium-dependent protein kinase 26	0/1	0	0	0	No
Q38872	CDPK6_ARATH	OMA	A. thaliana	CPK6, CDPK3, At2g17290, F5J6.13	Calcium-dependent protein kinase 6, EC 2.7.11.1	0/1	0	0	0	Yes
Q7XJR9	CDPKG_ARATH	OMA	A. thaliana	CPK16, At2g17890, T13L16.9	Calcium-dependent protein kinase 16, EC 2.7.11.1	0/1	0	0	0	Yes
Q9SIQ7	CDPKO_ARATH	OMA	A. thaliana	CPK24, At2g31500, T28P16.1, T9H9.2	Calcium-dependent protein kinase 24, EC 2.7.11.1	0/1	0	0	0	Yes
Q9SJ61	CDPKP_ARATH	OMA	A. thaliana	CPK25, At2g35890, F11F19.20	Calcium-dependent protein kinase 25, EC 2.7.11.1	0/1	0	0	0	Yes
Q9ZV15	CDPKK_ARATH	OMA	A. thaliana	CPK20, At2g38910, T7F6.8	Calcium-dependent protein kinase 20, EC 2.7.11.1	0/1	0	0	0	Yes
P93759	CDPKE_ARATH	OMA	A. thaliana	CPK14, At2g41860, T11A7.4, T6D20.24	Calcium-dependent protein kinase 14, EC 2.7.11.1	0/1	0	0	0	Yes
Q38870	CDPK2_ARATH	OMA	A. thaliana	CPK2, At3g10660, F13M14.5, F18K10.28	Calcium-dependent protein kinase 2, EC 2.7.11.1	0/1	0	0	0	Yes
Q38868	CDPK9_ARATH	OMA	A. thaliana	CPK9, At3g20410, MQC12.23	Calcium-dependent protein kinase 9, EC 2.7.11.1	0/1	0	0	0	Yes
Q06850	CDPK1_ARATH	OMA	A. thaliana	CPK1, AK1, At5g04870, MUK11.19	Calcium-dependent protein kinase 1, AtCDPK 1, CDPK 1, EC 2.7.11.1	0/1	0	0	0	Yes
Q9FMP5	CDPKH_ARATH	OMA	A. thaliana	CPK17, At5g12180, MXC9.14	Calcium-dependent protein kinase 17, EC 2.7.11.1	0/1	0	0	0	Yes
Q38873	CDPK7_ARATH	OMA	A. thaliana	CPK7, At5g12480, F7K24.200	Calcium-dependent protein kinase 7, EC 2.7.11.1	0/1	0	0	0	Yes
Q3E9C0	CDPKY_ARATH	OMA	A. thaliana	CPK34, At5g19360, F7K24.110	Calcium-dependent protein kinase 34, EC 2.7.11.1	0/1	0	0	0	Yes
Q42396	CDPKC_ARATH	OMA	A. thaliana	CPK12, CDPK9, At5g23580, MQM1.15	Calcium-dependent protein kinase 12, EC 2.7.11.1	0/1	0	0	0	No
Q9FKW4	CDPKS_ARATH	OMA	A. thaliana	CPK28, At5g66210, K2A18.29	Calcium-dependent protein kinase 28, EC 2.7.11.1	1/1	0	1	0	No
Q9M9V8	CDPKA_ARATH	OMA	A. thaliana	CPK10, CDPK1, At1g18890, F6A14.1	Calcium-dependent protein kinase 10, EC 2.7.11.1	0/1	0	0	0	Yes
Q39016	CDPKB_ARATH	OMA	A. thaliana	CPK11, CDPK2, At1g35670, F15O4.8	Calcium-dependent protein kinase 11, EC 2.7.11.1	0/1	0	0	0	No
Q9C6P3	CDPKX_ARATH	OMA	A. thaliana	CPK33, At1g50700, F17J6.22	Calcium-dependent protein kinase 33, EC 2.7.11.1	0/1	0	0	0	Yes
Q9SSF8	CDPKU_ARATH	OMA	A. thaliana	CPK30, CDPK1A, At1g74740, F25A4.29	Calcium-dependent protein kinase 30, EC 2.7.11.1	0/1	0	0	0	Yes
F4I0S0	F4I0S0_ARATH	OMA	A. thaliana	At1g76040, CPK29, AT1G76040	Calcium-dependent protein kinase 29	0/1	0	0	0	No
Q3HNM6	Q3HNM6_TOXGO	InParanoid, OMA	T. gondii	TGRH88_017420	Calcium-dependent protein kinase CDPK3	1/2	0	1	0	Yes
P08413	KCC2B_RAT	InParanoid	R. norvegicus	Camk2b	Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CaMK-II subunit beta, EC 2.7.11.17	0/3	0	0	0	Yes
P15791	KCC2D_RAT	InParanoid	R. norvegicus	Camk2d	Calcium/calmodulin-dependent protein kinase type II subunit delta, CaM kinase II subunit delta, CaMK-II subunit delta, EC 2.7.11.17	0/3	0	0	0	Yes
P11730	KCC2G_RAT	InParanoid	R. norvegicus	Camk2g	Calcium/calmodulin-dependent protein kinase type II subunit gamma, CaM kinase II subunit gamma, CaMK-II subunit gamma, EC 2.7.11.17	0/3	0	0	0	Yes
P11275	KCC2A_RAT	InParanoid	R. norvegicus	Camk2a	Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, EC 2.7.11.17	0/3	1	0	0	Yes
Q9P7I2	KCC1_SCHPO	InParanoid	S. pombe	cmk1, SPAC25D11.02c, SPACUNK12.02c	Calcium/calmodulin-dependent protein kinase type I	1/1	0	1	0	No
P22517	KCC2_YEAST	InParanoid	S. cerevisiae S288c	CMK2, YOL016C	Calcium/calmodulin-dependent protein kinase II, EC 2.7.11.17	0/1	0	0	0	No
P27466	KCC1_YEAST	InParanoid	S. cerevisiae S288c	CMK1, YFR014C	Calcium/calmodulin-dependent protein kinase I, EC 2.7.11.17	0/1	0	0	0	No

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:1334	Siddiqui M et al.	2020	Plasmodium falciparum (isolate 3D7)	Whole organism	Total cell lysate	2BP inhibition, ABE, ARAC, NH2OH cleavage of thioester bond, Thioesterases drug inhibition

Cysteine summary and predictions

Pos in alignment	3	73	105	166	180	248	253	262	341	445	519
Subcell. location	C	C	C	C	C	C	C	C	C	C	C
Disulfide	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	coil	strand	coil	helix	helix	helix	helix	helix	helix	helix	helix
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys³ HC HC	Cys⁷³	Cys¹⁰⁵	Cys¹⁶⁶	Cys¹⁸⁰	Cys²⁴⁸	Cys²⁵³	Cys²⁶²	Cys³⁴¹	Cys⁴⁴⁵	Cys⁵¹⁹

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

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ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	x		HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	x		HC: 162 hits LC: 218 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	x		HC: 102 hits LC: 73 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	x		HC HC: 352 hits LC LC: 8 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	Found	in HC	HC: 79 hits LC: 89 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	Found	in HC	HC: 100 hits LC: 217 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	Ortholog	in List [KCC2B, KCC2A, KCC2G, KCC2D]	List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	Ortholog	in MC,LC [CDPK8, CDPKW, CDPKV, CDPKS, CDPKD, CDPKL]	HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	Ortholog	in List [KCC2B, KCC2A, KCC2G, KCC2D]	List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	Ortholog	in List [KCC1]	List: 238 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	x		List: 436 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	x		Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	x		List: 354 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Ortholog	in List [KCC2B, KCC2G, KCC2D]	List: 1348 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	x		List: 354 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	x		List: 226 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	x		List: 274 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	x		List: 1353 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	Ortholog	in HC [KCC2B, KCC2A, KCC2G, KCC2D]	HC: 1079 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		List: 865 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	x		List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	x		List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	Ortholog	in HC [KCC2B, KCC2A, KCC2G, KCC2D]	HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		LPS treatment: 646 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		List: 646 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	Ortholog	in List [KCC2B, KCC2G, KCC2D]	List: 2929 hits
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 590 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [KCC2B, KCC2A, KCC2G, KCC2D]	List: 370 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [KCC2B, KCC2G, KCC2D]	HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [KCC2B, KCC2G, KCC2D]	HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 600 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		List: 185 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [KCC2B, KCC2G, KCC2D]	HC: 1869 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [KCC2B, KCC2A, KCC2G, KCC2D]	List: 1093 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [KCC2B, KCC2G, KCC2D]	List: 687 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Ortholog	in List [KCC2B, KCC2G, KCC2D]	List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 199 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 399 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [KCC2B, KCC2A, KCC2G, KCC2D]	List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [KCC2B, KCC2A, KCC2G, KCC2D]	List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [KCC2B, KCC2A, KCC2G, KCC2D]	List: 1040 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 464 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 4107 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [KCC2B, KCC2A, KCC2G, KCC2D]	List: 1597 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [KCC2B, KCC2A, KCC2G, KCC2D]	List: 1378 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 3870 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	0	0.0	NA
H. sapiens	22	38	7	18.4	KCC2B_HUMAN , KCC2G_HUMAN , KCC2D_HUMAN
M. musculus	23	37	11	29.7	KCC2B_MOUSE , KCC2A_MOUSE , KCC2G_MOUSE , KCC2D_MOUSE
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	2	100.0	NA
A. thaliana	1	1	1	100.0	CDPK8_ARATH , CDPKW_ARATH , CDPKV_ARATH , CDPKS_ARATH , CDPKD_ARATH , CDPKL_ARATH
S. pombe	1	1	1	100.0	KCC1_SCHPO
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	0	0.0	NA
C. familiaris	1	1	0	0.0	NA

References

Authors	Year	Reference title	PMID
Kang R et al.	2008	Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.	19092927
Ren W et al.	2013	Proteomic analysis of protein palmitoylation in adipocytes.	23599907
Marin EP et al.	2012	Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases.	22496122
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
Martin BR et al.	2011	Global profiling of dynamic protein palmitoylation.	22056678
Dowal L et al.	2011	Proteomic analysis of palmitoylated platelet proteins.	21813449
Merrick BA et al.	2011	Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3.	21785166
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
Forrester MT et al.	2011	Site-specific analysis of protein S-acylation by resin-assisted capture.	21044946
Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
Yang W et al.	2010	Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes.	19801377
Martin BR et al.	2009	Large-scale profiling of protein palmitoylation in mammalian cells.	19137006
Roth AF et al.	2006	Global analysis of protein palmitoylation in yeast.	16751107
Hemsley PA et al.	2013	A proteomic approach identifies many novel palmitoylated proteins in Arabidopsis.	23252521
Ivaldi C et al.	2012	Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.	22615937
Wan J et al.	2013	Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease.	24211138
Zhang MM et al.	2013	Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.	23843742
Jones ML et al.	2012	Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis.	22901544
Wei X et al.	2014	Insulin-regulated protein palmitoylation impacts endothelial cell function.	24357059
Serwa RA et al.	2015	Systems Analysis of Protein Fatty Acylation in Herpes Simplex Virus-Infected Cells Using Chemical Proteomics.	26256475
Morrison E et al.	2015	Quantitative analysis of the human T cell palmitome.	26111759
Chesarino NM et al.	2014	Chemoproteomics reveals Toll-like receptor fatty acylation.	25371237
Santiago-Tirado FH et al.	2015	A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.	25970403
Emmer BT et al.	2011	Global analysis of protein palmitoylation in African trypanosomes.	21193548
Colquhoun DR et al.	2015	Bioorthogonal mimetics of palmitoyl-CoA and myristoyl-CoA and their subsequent isolation by click chemistry and characterization by mass spectrometry reveal novel acylated host-proteins modified by HIV-1 infection.	25914232
Foe IT et al.	2015	Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.	26468752
Gould NS et al.	2015	Site-Specific Proteomic Mapping Identifies Selectively Modified Regulatory Cysteine Residues in Functionally Distinct Protein Networks.	26165157
Peng T et al.	2015	Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.	25575299
Pinner AL et al.	2016	Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia.	26876311
Hernandez JL et al.	2016	Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.	27030425
Fang C et al.	2016	Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody.	26865113
Caballero MC et al.	2016	Identification of new palmitoylated proteins in Toxoplasma gondii.	26825284
Upadhyay S et al.	2016	Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation.	27879337
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
Sobocińska J et al.	2018	Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.	29217618
Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Thinon E et al.	2016	Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.	27350074
Thinon E et al.	2018	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.	29575903
Zhang X et al.	2018	Ultradeep Palmitoylomics Enabled by Dithiodipyridine-Functionalized Magnetic Nanoparticles.	29660268
Won SJ et al.	2018	Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.	29733200
Dumoulin A et al.	2018	S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.	30319353
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Wang J et al.	2019	DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane.	30605677
Strassburger K et al.	2019	Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.	30735487
Zhou B et al.	2019	Low-background Acyl-biotinyl Exchange Largely Eliminates the Co-isolation of Non-S-acylated Proteins and Enables Deep S-acylproteomic Analysis.	31251020
Zaręba-Kozioł M et al.	2019	Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins.	31311849
Wegleiter T et al.	2019	Palmitoylation of BMPR1a regulates neural stem cell fate.	31772009
Siddiqui M et al.	2020	Protein S-palmitoylation is responsive to external signals and plays a regulatory role in microneme secretion in Plasmodium falciparum merozoites.	32003970
Morrison E et al.	2020	Dynamic palmitoylation events following T-cell receptor signaling.	32651440
Mariscal J et al.	2020	Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles.	32944167
Gorenberg EL et al.	2022	Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.	35358180
Uzbekova S et al.	2021	Protein Palmitoylation in Bovine Ovarian Follicle.	34769186
Miles MR et al.	2021	Global identification of S-palmitoylated proteins and detection of palmitoylating (DHHC) enzymes in heart.	33636221
Pradhan AJ et al.	2021	Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.	33848465
Ripamonti M et al.	2021	Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions.	33782527
Zaręba-Kozioł M et al.	2021	S-Palmitoylation of Synaptic Proteins as a Novel Mechanism Underlying Sex-Dependent Differences in Neuronal Plasticity.	34200797
Ziemlińska E et al.	2021	Palm Oil-Rich Diet Affects Murine Liver Proteome and <i>S</i>-Palmitoylome.	34884899
Gao J et al.	2022	Proteome-wide identification of palmitoylated proteins in mouse testis.	35477839