Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. 4-nitrophenol + H(+) + NADPH + O2 = 4-nitrocatechol + H2O + NADP(+) Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria. Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein. Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female. Detectable in the female liver on day 1 and reaches steady state levels on days 16-20. By ethanol and acetone in the liver and by testosterone in the kidney and adrenal tissues. Belongs to the cytochrome P450 family. A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics. an organic molecule + O2 + reduced [NADPH--hemoprotein reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] (5Z,8Z,11Z)-eicosatrienoate + O2 + reduced [NADPH--hemoprotein reductase] = 19-hydroxy-(5Z,8Z,11Z)-eicosatrienoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + O2 + reduced [NADPH--hemoprotein reductase] = 19-hydroxy-(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 + reduced [NADPH--hemoprotein reductase] = 21-hydroxy-(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] dodecanoate + O2 + reduced [NADPH--hemoprotein reductase] = 11-hydroxydodecanoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] O2 + reduced [NADPH--hemoprotein reductase] + tetradecanoate = 13-hydroxytetradecanoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] 4-nitrophenol + H(+) + NADPH + O2 = 4-nitrocatechol + H2O + NADP(+) The omega-1 hydroxylase activity is stimulated by cytochrome b5. Lipid metabolism; fatty acid metabolism. Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria. Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein. Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female. Detectable in the female liver on day 1 and reaches steady state levels on days 16-20. By ethanol and acetone in the liver and by testosterone in the kidney and adrenal tissues. Belongs to the cytochrome P450 family. Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. 4-nitrophenol + NADPH + O(2) = 4-nitrocatechol + NADP(+) + H(2)O. Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female. Detectable in the female liver on day 1 and reaches steady state levels on days 16-20. By ethanol and acetone in the liver and by testosterone in the kidney and adrenal tissues. Belongs to the cytochrome P450 family.