SwissPalm

Protein general information from UniProt

Protein names

Calcium-activated potassium channel subunit alpha-1; BK channel; BKCA alpha; Calcium-activated potassium channel, subfamily M subunit alpha-1; K; VCAalpha; KCa1.1; Maxi K channel; MaxiK; Slo-alpha; Slo1; mSlo1; Slowpoke homolog; Slo homolog; mSlo

Subcellular location

Cell membrane (By similarity)

Isoforms & protein length

Isoform 1 (main): 1209 aa; Isoform 2 : 1196 aa; Isoform 3 : 1178 aa; Isoform 4 : 1236 aa; Isoform 5 : 1144 aa

Known functions

Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). Ethanol and carbon monoxide-bound heme increase channel activation. Heme inhibits channel activation (By similarity). Homotetramer; which constitutes the calcium-activated potassium channel. Interacts with beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Interacts with gamma subunits LRRC26, LRRC38, LRRC52 and LRRC55. Beta and gamma subunits are accessory, and modulate its activity (By similarity). Interacts with RAB11B. Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). Ethanol and carbon monoxide-bound heme increase channel activation. Heme inhibits channel activation (By similarity). Homotetramer; which constitutes the calcium-activated potassium channel. Interacts with beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Interacts with gamma subunits LRRC26, LRRC38, LRRC52 and LRRC55. Beta and gamma subunits are accessory, and modulate its activity (By similarity). Interacts with RAB11B.

Cross-references

Source	Identifiers
UniProt gene names	Kcnma, Kcnma1
UniProt secondary AC	Q9R196, Q8VHF1, Q64703
Refseq protein ID	NP_034740.2, NP_001240298.1, NP_001240294.1
IPI ID	IPI00314099, IPI00466539, IPI00975115, IPI00410904, IPI00410903, IPI00410901, IPI00120643

Source	Identifiers
MGI ID	MGI:99923

Enzymes

PAT/APT	Enzyme	Experiments
PAT	ZDH23_MOUSE	SPalmE:1273 Duncan PJ et al. (2019) [] SPalmE:1272 Duncan PJ et al. (2019) [] SPalmE:291 Tian L et al. (2012)
PAT	ZDH22_MOUSE	SPalmE:291 Tian L et al. (2012)
APT	LYPA1_HUMAN	SPalmE:291 Tian L et al. (2012)
APT	LYPL1_HUMAN	SPalmE:291 Tian L et al. (2012)

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

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UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Predicted
Q28204	KCMA1_BOVIN	InParanoid	B. taurus	KCNMA, KCNMA1	Calcium-activated potassium channel subunit alpha-1	0/2	0	0	Yes
Q28265	KCMA1_CANLF	InParanoid	C. familiaris	KCNMA, KCNMA1	Calcium-activated potassium channel subunit alpha-1	0/1	0	0	Yes
Q12791	KCMA1_HUMAN	MetaPhors	H. sapiens	SLO, KCNMA, KCNMA1	Calcium-activated potassium channel subunit alpha-1	1/22	1	1	Yes
Q8IKI3	Q8IKI3_PLAF7	InParanoid	P. falciparum	PF3D7_1465500	Potassium channel	0/1	0	0	Yes
Q62976	KCMA1_RAT	InParanoid	R. norvegicus	Kcnma, Kcnma1	Calcium-activated potassium channel subunit alpha-1	1/3	0	1	Yes
Q03720	SLO_DROME	InParanoid	D. melanogaster	CG10693, slo	Calcium-activated potassium channel slowpoke	0/1	0	0	Yes

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:205	Jeffries O et al.	2010	Mus musculus	HEK293T	Plasma Membrane	2BP inhibition, Point mutation, Substrate localization by fluorescence tag, [3H]palmitic acid labeling
SPalmE:291	Tian L et al.	2012	Mus musculus	HEK293T	Plasma Membrane	, , Depletion of PAT by siRNA, PAT overexpression, Point mutation, Substrate localization by fluorescence tag, Thioesterases overexpression, [3H]palmitic acid labeling
SPalmE:1272	Duncan PJ et al.	2019	Mus musculus	Vascular smooth muscle cell	Total cell lysate	ARAC, PAT KO, Point mutation
SPalmE:1273	Duncan PJ et al.	2019	Homo sapiens	HEK293	Total cell lysate	PAT KO, Point mutation, [3H]palmitic acid labeling

Sites (from validation or large-scale experiments)

Isoforms	Position	Probability	Studies	Techniques
Q08460-5	53	NA	SPalmE:205 Jeffries O et al. (2010) SPalmE:291 Tian L et al. (2012)	, , 2BP inhibition, Depletion of PAT by siRNA, PAT overexpression, Point mutation, Substrate localization by fluorescence tag, Thioesterases overexpression, [3H]palmitic acid labeling
Q08460-5	54	NA	SPalmE:205 Jeffries O et al. (2010) SPalmE:291 Tian L et al. (2012)	, , 2BP inhibition, Depletion of PAT by siRNA, PAT overexpression, Point mutation, Substrate localization by fluorescence tag, Thioesterases overexpression, [3H]palmitic acid labeling
Q08460-5	56	NA	SPalmE:205 Jeffries O et al. (2010) SPalmE:291 Tian L et al. (2012)	, , 2BP inhibition, Depletion of PAT by siRNA, PAT overexpression, Point mutation, Substrate localization by fluorescence tag, Thioesterases overexpression, [3H]palmitic acid labeling
Q08460	79	NA	SPalmE:1273 Duncan PJ et al. (2019) [] SPalmE:1272 Duncan PJ et al. (2019) []	ARAC, PAT KO, Point mutation, [3H]palmitic acid labeling

Cysteine summary and predictions

Pos in alignment	79	118	119	121	206	342	413	487	495	550	563	619	622	681
Subcell. location	E	C	C	C	E	U	C	C	C	C	C	C	C	C
Disulfide	no	no	no	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	coil	helix	coil	coil	coil	helix	coil	strand	coil	strand	coil	helix	helix	helix
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁷⁹	Cys¹¹⁸ HC	Cys¹¹⁹ HC	Cys¹²¹ HC	Cys²⁰⁶ HC	Cys³⁴²	Cys⁴¹³	Cys⁴⁸⁷	Cys⁴⁹⁵	Cys⁵⁵⁰	Cys⁵⁶³	Cys⁶¹⁹	Cys⁶²²	Cys⁶⁸¹
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys⁴¹	Cys⁸⁰ HC MC	Cys⁸¹ HC LC	Cys⁸³ HC	Cys¹⁶⁸ HC	Cys³⁰⁴	Cys³⁷⁵	Cys⁴⁴⁹	Cys⁴⁵⁷	Cys⁵¹²	Cys⁵²⁵	Cys⁵⁸¹	Cys⁵⁸⁴	Cys⁶³⁹
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys⁷⁹	Cys¹¹⁸ HC	Cys¹¹⁹ HC	Cys¹²¹ HC	Cys²⁰⁶ HC	Cys³⁴²	Cys⁴¹³	Cys⁴⁸⁷	Cys⁴⁹⁵	Cys⁵⁵⁰	Cys⁵⁶³	Cys⁶¹⁹	Cys⁶²²	Cys⁶⁷⁷
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys⁷⁹	Cys¹¹⁸ HC LC	Cys¹¹⁹ HC	Cys¹²¹ HC	Cys²⁰⁶ HC	Cys³⁴²	Cys⁴¹³	Cys⁴⁸⁷	Cys⁴⁹⁵	Cys⁵⁵⁰	Cys⁵⁶³	Cys⁶¹⁹	Cys⁶²²	Cys⁶⁷⁷
Pos in isoform 5 CSS-Palm 4.0 PalmPred	Cys¹⁴ HC	Cys⁵³ HC LC	Cys⁵⁴ HC	Cys⁵⁶ HC	Cys¹⁴¹ HC	Cys²⁷⁷	Cys³⁴⁸	Cys⁴²²	Cys⁴³⁰	Cys⁴⁸⁵	Cys⁴⁹⁸	Cys⁵⁵⁴	Cys⁵⁵⁷	Cys⁶¹⁶

Pos in alignment	684	697	699	714	715	718	725	727	753	822	849	924	927	947
Subcell. location	C	C	C	U	U	U	U	U	U	C	C	C	C	C
Disulfide	no	no	no							no	no	no	no	no
PSIPRED v.4.02 prediction	coil	coil	coil
UniProt secondary structure	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁶⁸⁴	Cys⁶⁹⁷	Cys⁶⁹⁹							Cys⁷⁶⁴	Cys⁷⁹¹	Cys⁸⁶⁶	Cys⁸⁶⁹	Cys⁸⁸⁹
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys⁶⁴²	Cys⁶⁵⁵	Cys⁶⁵⁷							Cys⁷²⁵	Cys⁷⁵²	Cys⁸²⁷	Cys⁸³⁰	Cys⁸⁵⁰
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys⁶⁸⁰	Cys⁶⁹³	Cys⁶⁹⁵							Cys⁷⁶⁰	Cys⁷⁸⁷	Cys⁸⁶²	Cys⁸⁶⁵	Cys⁸⁸⁵
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys⁶⁸⁰	Cys⁶⁹³	Cys⁶⁹⁵	Cys⁷¹⁰ HC	Cys⁷¹¹ HC	Cys⁷¹⁴	Cys⁷²¹ LC	Cys⁷²³	Cys⁷⁴⁹	Cys⁸¹⁸	Cys⁸⁴⁵	Cys⁹²⁰	Cys⁹²³	Cys⁹⁴³
Pos in isoform 5 CSS-Palm 4.0 PalmPred	Cys⁶¹⁹	Cys⁶³²	Cys⁶³⁴							Cys⁶⁹⁹	Cys⁷²⁶	Cys⁸⁰¹	Cys⁸⁰⁴	Cys⁸²⁴

Pos in alignment	1065	1129	1149	1155	1165	1182	1205	1320
Subcell. location	C	C	C	C	C	C	C	U
Disulfide	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction
UniProt secondary structure
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys¹⁰⁰⁷	Cys¹⁰⁷¹	Cys¹⁰⁹¹	Cys¹⁰⁹⁷ HC	Cys¹¹⁰⁷ HC	Cys¹¹²⁴	Cys¹¹⁴⁷
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys⁹⁴¹	Cys¹⁰⁰⁵	Cys¹⁰²⁵	Cys¹⁰³¹ HC	Cys¹⁰⁴¹ HC	Cys¹⁰⁵⁸	Cys¹⁰⁸¹	Cys¹¹⁹⁶
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys⁹⁷⁶	Cys¹⁰⁴⁰	Cys¹⁰⁶⁰	Cys¹⁰⁶⁶ HC	Cys¹⁰⁷⁶ HC	Cys¹⁰⁹³	Cys¹¹¹⁶
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys¹⁰³⁴	Cys¹⁰⁹⁸	Cys¹¹¹⁸	Cys¹¹²⁴ HC	Cys¹¹³⁴ HC	Cys¹¹⁵¹	Cys¹¹⁷⁴
Pos in isoform 5 CSS-Palm 4.0 PalmPred	Cys⁹⁴²	Cys¹⁰⁰⁶	Cys¹⁰²⁶	Cys¹⁰³² HC	Cys¹⁰⁴² HC	Cys¹⁰⁵⁹	Cys¹⁰⁸²

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

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ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	Ortholog	in LC [KCMA1]	HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	x		HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	x		HC: 102 hits LC: 73 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	x		HC: 162 hits LC: 218 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	x		HC HC: 352 hits LC LC: 8 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	x		HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	x		Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	x		List: 436 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Ortholog	in List [KCMA1]	List: 1348 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	x		List: 274 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	x		List: 226 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	x		List: 354 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	x		List: 354 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	x		List: 1353 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	x		HC: 1079 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		List: 865 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		List: 646 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		LPS treatment: 646 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	x		List: 1404 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	x		List: 1394 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	x
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	1	25.0	KCMA1_RAT
H. sapiens	22	37	1	2.7	KCMA1_HUMAN
M. musculus	23	37	0	0.0	NA
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
A. thaliana	1	1	0	0.0	NA
S. pombe	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	0	0.0	NA
C. familiaris	1	1	0	0.0	NA

References

Authors	Year	Reference title	PMID
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Colquhoun DR et al.	2015	Bioorthogonal mimetics of palmitoyl-CoA and myristoyl-CoA and their subsequent isolation by click chemistry and characterization by mass spectrometry reveal novel acylated host-proteins modified by HIV-1 infection.	25914232
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
Thinon E et al.	2016	Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.	27350074
Kang R et al.	2008	Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.	19092927
Wang J et al.	2019	DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane.	30605677
Yang W et al.	2010	Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes.	19801377
Zaręba-Kozioł M et al.	2021	S-Palmitoylation of Synaptic Proteins as a Novel Mechanism Underlying Sex-Dependent Differences in Neuronal Plasticity.	34200797
Upadhyay S et al.	2016	Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation.	27879337
Won SJ et al.	2018	Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.	29733200
Wegleiter T et al.	2019	Palmitoylation of BMPR1a regulates neural stem cell fate.	31772009
Jeffries O et al.	2010	Palmitoylation of the S0-S1 linker regulates cell surface expression of voltage- and calcium-activated potassium (BK) channels.	20693285
Pinner AL et al.	2016	Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia.	26876311
Mariscal J et al.	2020	Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles.	32944167
Dowal L et al.	2011	Proteomic analysis of palmitoylated platelet proteins.	21813449
Martin BR et al.	2009	Large-scale profiling of protein palmitoylation in mammalian cells.	19137006
Chesarino NM et al.	2014	Chemoproteomics reveals Toll-like receptor fatty acylation.	25371237
Ivaldi C et al.	2012	Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.	22615937
Zhou B et al.	2019	Low-background Acyl-biotinyl Exchange Largely Eliminates the Co-isolation of Non-S-acylated Proteins and Enables Deep S-acylproteomic Analysis.	31251020
Caballero MC et al.	2016	Identification of new palmitoylated proteins in Toxoplasma gondii.	26825284
Miles MR et al.	2021	Global identification of S-palmitoylated proteins and detection of palmitoylating (DHHC) enzymes in heart.	33636221
Wei X et al.	2014	Insulin-regulated protein palmitoylation impacts endothelial cell function.	24357059
Dumoulin A et al.	2018	S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.	30319353
Forrester MT et al.	2011	Site-specific analysis of protein S-acylation by resin-assisted capture.	21044946
Zhang MM et al.	2013	Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.	23843742
Zhang X et al.	2018	Ultradeep Palmitoylomics Enabled by Dithiodipyridine-Functionalized Magnetic Nanoparticles.	29660268
Peng T et al.	2015	Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.	25575299
Gao J et al.	2022	Proteome-wide identification of palmitoylated proteins in mouse testis.	35477839
Wan J et al.	2013	Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease.	24211138
Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
Pradhan AJ et al.	2021	Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.	33848465
Morrison E et al.	2020	Dynamic palmitoylation events following T-cell receptor signaling.	32651440
Sobocińska J et al.	2018	Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.	29217618
Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
Santiago-Tirado FH et al.	2015	A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.	25970403
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
Strassburger K et al.	2019	Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.	30735487
Martin BR et al.	2011	Global profiling of dynamic protein palmitoylation.	22056678
Duncan PJ et al.	2019	<i>S</i>-Acylation controls functional coupling of BK channel pore-forming α-subunits and β1-subunits.	31213527
Ziemlińska E et al.	2021	Palm Oil-Rich Diet Affects Murine Liver Proteome and <i>S</i>-Palmitoylome.	34884899
Jones ML et al.	2012	Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis.	22901544
Foe IT et al.	2015	Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.	26468752
Gould NS et al.	2015	Site-Specific Proteomic Mapping Identifies Selectively Modified Regulatory Cysteine Residues in Functionally Distinct Protein Networks.	26165157
Serwa RA et al.	2015	Systems Analysis of Protein Fatty Acylation in Herpes Simplex Virus-Infected Cells Using Chemical Proteomics.	26256475
Hernandez JL et al.	2016	Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.	27030425
Zaręba-Kozioł M et al.	2019	Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins.	31311849
Thinon E et al.	2018	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.	29575903
Uzbekova S et al.	2021	Protein Palmitoylation in Bovine Ovarian Follicle.	34769186
Ripamonti M et al.	2021	Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions.	33782527
Morrison E et al.	2015	Quantitative analysis of the human T cell palmitome.	26111759
Tian L et al.	2012	Distinct acyl protein transferases and thioesterases control surface expression of calcium-activated potassium channels.	22399288
Marin EP et al.	2012	Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases.	22496122
Merrick BA et al.	2011	Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3.	21785166
Emmer BT et al.	2011	Global analysis of protein palmitoylation in African trypanosomes.	21193548
Ren W et al.	2013	Proteomic analysis of protein palmitoylation in adipocytes.	23599907
Gorenberg EL et al.	2022	Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.	35358180
Fang C et al.	2016	Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody.	26865113
Roth AF et al.	2006	Global analysis of protein palmitoylation in yeast.	16751107
Hemsley PA et al.	2013	A proteomic approach identifies many novel palmitoylated proteins in Arabidopsis.	23252521

Q08460 KCMA1_MOUSE