SwissPalm

Protein general information from UniProt

Protein names

Calcium-activated potassium channel subunit alpha-1; BK channel; BKCA alpha; Calcium-activated potassium channel, subfamily M subunit alpha-1; K; VCAalpha; KCa1.1; Maxi K channel; MaxiK; Slo-alpha; Slo1; Slowpoke homolog; Slo homolog; hSlo

Subcellular location

Cell membrane (By similarity)

Isoforms & protein length

Isoform 1 (main): 1236 aa; Isoform 2 : 1219 aa; Isoform 3 : 1240 aa; Isoform 4 : 1207 aa; Isoform 5 : 1178 aa; Isoform 6 : 168 aa; Isoform 7 : 1239 aa; Isoform 7 : 1239 aa

Known functions

Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). Ethanol and carbon monoxide-bound heme increase channel activation. Heme inhibits channel activation. Homotetramer; which constitutes the calcium-activated potassium channel. Interacts with RAB11B (By similarity). Interacts with beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Interacts with gamma subunits LRRC26, LRRC38, LRRC52 and LRRC55. Beta and gamma subunits are accessory, and modulate its activity. Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). Ethanol and carbon monoxide-bound heme increase channel activation. Heme inhibits channel activation. Homotetramer; which constitutes the calcium-activated potassium channel. Interacts with RAB11B (By similarity). Interacts with beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Interacts with gamma subunits LRRC26, LRRC38, LRRC52 and LRRC55. Beta and gamma subunits are accessory, and modulate its activity.

Cross-references

Source	Identifiers
UniProt gene names	SLO, KCNMA, KCNMA1
UniProt secondary AC	Q9UQK6, Q9UCX0, Q9UBB0, Q96LG8, Q5SQR9, Q5JQ23, Q13150, Q12960, Q12921, Q12917, Q12886, F8WA96
Refseq protein ID	NP_002238.2, NP_001258451.1, NP_001258447.1, NP_001154825.1, NP_001154824.1, NP_001014797.1
IPI ID	IPI00086846, IPI00102691, IPI00146484, IPI00156620, IPI00157070, IPI00184674, IPI00332285, IPI00395380, IPI00410167, IPI00555740, IPI00556229, IPI00645064, IPI00645602, IPI00647276, IPI00816528, IPI00844030, IPI00873626, IPI00874101, IPI00937747, IPI00941586, IPI00953323, IPI00478411, IPI00410173, IPI00410172, IPI00410170, IPI00410169, IPI00410168, IPI00164387

Source	Identifiers
Ensembl protein	ENSP00000474686, ENSP00000385806, ENSP00000346321, ENSP00000286628, ENSP00000286627, ENSP00000485867
Ensembl gene	ENSG00000156113

Enzymes

PAT/APT	Enzyme	Experiments
APT	AB17A_HUMAN	SPalmE:1393 McClafferty H et al. (2020) []
APT	AB17C_HUMAN	SPalmE:1393 McClafferty H et al. (2020) []

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

Select all Deselect all

UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Sites	Predicted
Q28204	KCMA1_BOVIN	Ensembl, InParanoid	B. taurus	KCNMA, KCNMA1	Calcium-activated potassium channel subunit alpha-1	0/1	0	0	0	Yes
Q08460	KCMA1_MOUSE	InParanoid, MetaPhors	M. musculus	Kcnma, Kcnma1	Calcium-activated potassium channel subunit alpha-1	0/17	4	0	4	Yes
Q62976	KCMA1_RAT	Ensembl, InParanoid	R. norvegicus	Kcnma, Kcnma1	Calcium-activated potassium channel subunit alpha-1	1/2	0	1	0	Yes
Q03720	SLO_DROME	Ensembl, InParanoid	D. melanogaster	CG10693, slo	Calcium-activated potassium channel slowpoke	0/1	0	0	0	Yes

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:1393	McClafferty H et al.	2020	Homo sapiens	HEK293	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, Point mutation, Thioesterases overexpression

Cysteine summary and predictions

Pos in alignment	79	118	119	121	206	342	413	487	495	550	563	619	622	681
Subcell. location	E	C	C	C	E	U	C	C	C	C	C	C	C	C
Disulfide	no	no	no	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	coil	helix	coil	coil	coil	helix	coil	strand	coil	strand	coil	helix	helix
UniProt secondary structure	NA	NA	NA	NA	NA	NA	strand	strand	NA	strand	helix	helix	helix
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁷⁹	Cys¹¹⁸ HC	Cys¹¹⁹ HC	Cys¹²¹ HC	Cys²⁰⁶ HC	Cys³⁴²	Cys⁴¹³	Cys⁴⁸⁷	Cys⁴⁹⁵	Cys⁵⁵⁰	Cys⁵⁶³	Cys⁶¹⁹	Cys⁶²²	Cys⁶⁷⁷
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys⁷⁹	Cys¹¹⁸ HC	Cys¹¹⁹ HC	Cys¹²¹ HC	Cys²⁰⁶ HC	Cys³⁴²	Cys⁴¹³	Cys⁴⁸⁷	Cys⁴⁹⁵	Cys⁵⁵⁰	Cys⁵⁶³	Cys⁶¹⁹	Cys⁶²²	Cys⁶⁷⁷
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys⁷⁹	Cys¹¹⁸ HC	Cys¹¹⁹ HC	Cys¹²¹ HC	Cys²⁰⁶ HC	Cys³⁴²	Cys⁴¹³	Cys⁴⁸⁷	Cys⁴⁹⁵	Cys⁵⁵⁰	Cys⁵⁶³	Cys⁶¹⁹	Cys⁶²²	Cys⁶⁸¹
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys⁷⁹	Cys¹¹⁸ HC	Cys¹¹⁹ HC	Cys¹²¹ HC	Cys²⁰⁶ HC	Cys³⁴²	Cys⁴¹³	Cys⁴⁸⁷	Cys⁴⁹⁵	Cys⁵⁵⁰	Cys⁵⁶³	Cys⁶¹⁹	Cys⁶²²	Cys⁶⁷⁷
Pos in isoform 5 CSS-Palm 4.0 PalmPred	Cys⁷⁹	Cys¹¹⁸ HC	Cys¹¹⁹ HC	Cys¹²¹ HC	Cys²⁰⁶ HC	Cys³⁴²	Cys⁴¹³	Cys⁴⁸⁷	Cys⁴⁹⁵	Cys⁵⁵⁰	Cys⁵⁶³	Cys⁶¹⁹	Cys⁶²²	Cys⁶⁷⁷
Pos in isoform 6 CSS-Palm 4.0 PalmPred	Cys⁷⁹	Cys¹¹⁸ HC HC	Cys¹¹⁹ HC HC	Cys¹²¹ HC
Pos in isoform 7 CSS-Palm 4.0 PalmPred	Cys⁷⁹	Cys¹¹⁸ HC	Cys¹¹⁹ HC	Cys¹²¹ HC	Cys²⁰⁶ HC	Cys³⁴²	Cys⁴¹³	Cys⁴⁸⁷	Cys⁴⁹⁵	Cys⁵⁵⁰	Cys⁵⁶³	Cys⁶¹⁹	Cys⁶²²	Cys⁶⁷⁷

Pos in alignment	684	697	699	707	722	723	724	726	733	735	761	830	856	863
Subcell. location	C	C	C	U	C	C	U	C	C	C	C	C	U	U
Disulfide	no	no	no		no	no		no	no	no	no	no
PSIPRED v.4.02 prediction						coil
UniProt secondary structure						NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁶⁸⁰	Cys⁶⁹³	Cys⁶⁹⁵		Cys⁷¹⁰ HC	Cys⁷¹¹ HC		Cys⁷¹⁴	Cys⁷²¹ LC	Cys⁷²³ LC	Cys⁷⁴⁹	Cys⁸¹⁸
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys⁶⁸⁰	Cys⁶⁹³	Cys⁶⁹⁵									Cys⁷⁶⁰	Cys⁷⁸⁶	Cys⁷⁹³
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys⁶⁸⁴	Cys⁶⁹⁷	Cys⁶⁹⁹		Cys⁷¹⁴ HC	Cys⁷¹⁵ HC		Cys⁷¹⁸	Cys⁷²⁵ LC	Cys⁷²⁷ LC	Cys⁷⁵³	Cys⁸²²
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys⁶⁸⁰	Cys⁶⁹³	Cys⁶⁹⁵									Cys⁷⁸⁹
Pos in isoform 5 CSS-Palm 4.0 PalmPred	Cys⁶⁸⁰	Cys⁶⁹³	Cys⁶⁹⁵									Cys⁷⁶⁰
Pos in isoform 6 CSS-Palm 4.0 PalmPred
Pos in isoform 7 CSS-Palm 4.0 PalmPred	Cys⁶⁸⁰	Cys⁶⁹³	Cys⁶⁹⁵	Cys⁷⁰³			Cys⁷²⁰					Cys⁸²¹

Pos in alignment	898	973	976	996	1087	1151	1171	1177	1187	1204	1227
Subcell. location	C	C	C	C	C	C	C	C	C	C	C
Disulfide	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction						coil
UniProt secondary structure						NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁸⁴⁵	Cys⁹²⁰	Cys⁹²³	Cys⁹⁴³	Cys¹⁰³⁴	Cys¹⁰⁹⁸	Cys¹¹¹⁸	Cys¹¹²⁴ HC	Cys¹¹³⁴ HC	Cys¹¹⁵¹	Cys¹¹⁷⁴
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys⁸²⁸	Cys⁹⁰³	Cys⁹⁰⁶	Cys⁹²⁶	Cys¹⁰¹⁷	Cys¹⁰⁸¹	Cys¹¹⁰¹	Cys¹¹⁰⁷ HC	Cys¹¹¹⁷ HC	Cys¹¹³⁴	Cys¹¹⁵⁷
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys⁸⁴⁹	Cys⁹²⁴	Cys⁹²⁷	Cys⁹⁴⁷	Cys¹⁰³⁸	Cys¹¹⁰²	Cys¹¹²²	Cys¹¹²⁸ HC	Cys¹¹³⁸ HC	Cys¹¹⁵⁵	Cys¹¹⁷⁸
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys⁸¹⁶	Cys⁸⁹¹	Cys⁸⁹⁴	Cys⁹¹⁴	Cys¹⁰⁰⁵	Cys¹⁰⁶⁹	Cys¹⁰⁸⁹	Cys¹⁰⁹⁵ HC	Cys¹¹⁰⁵ HC	Cys¹¹²²	Cys¹¹⁴⁵
Pos in isoform 5 CSS-Palm 4.0 PalmPred	Cys⁷⁸⁷	Cys⁸⁶²	Cys⁸⁶⁵	Cys⁸⁸⁵	Cys⁹⁷⁶	Cys¹⁰⁴⁰	Cys¹⁰⁶⁰	Cys¹⁰⁶⁶ HC	Cys¹⁰⁷⁶ HC	Cys¹⁰⁹³	Cys¹¹¹⁶
Pos in isoform 6 CSS-Palm 4.0 PalmPred						Cys¹⁶⁵
Pos in isoform 7 CSS-Palm 4.0 PalmPred	Cys⁸⁴⁸	Cys⁹²³	Cys⁹²⁶	Cys⁹⁴⁶	Cys¹⁰³⁷	Cys¹¹⁰¹	Cys¹¹²¹	Cys¹¹²⁷ HC	Cys¹¹³⁷ HC	Cys¹¹⁵⁴	Cys¹¹⁷⁷

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

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ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	Ortholog	in LC [KCMA1]	HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	x		HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	x		HC: 102 hits LC: 73 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	x		HC: 162 hits LC: 218 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	x		HC HC: 352 hits LC LC: 8 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	x		HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	x		Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	x		List: 436 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Found	in List	List: 1348 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	x		List: 274 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	x		List: 226 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	x		List: 354 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	x		List: 354 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	x		List: 1353 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	x		HC: 1079 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		List: 865 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		List: 646 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		LPS treatment: 646 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	x		List: 1404 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	x		List: 1394 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	2	3	1	33.3	KCMA1_RAT
H. sapiens	17	26	1	3.8	NA
M. musculus	17	25	0	0.0	NA
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
A. thaliana	1	1	0	0.0	NA
S. pombe	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	1	1	0	0.0	NA
D. melanogaster	1	2	0	0.0	NA

References

Authors	Year	Reference title	PMID
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Colquhoun DR et al.	2015	Bioorthogonal mimetics of palmitoyl-CoA and myristoyl-CoA and their subsequent isolation by click chemistry and characterization by mass spectrometry reveal novel acylated host-proteins modified by HIV-1 infection.	25914232
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
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