SwissPalm

Protein general information from UniProt

Protein names

Protein scribble homolog, Scribble, hScrib; Protein LAP4

Subcellular location

Cell membrane (By similarity); Cell junction (Probable); adherens junction; Cell projection; lamellipodium; Cytoplasm (Probable); Adherens junction (Potential); Lamellipodium (Potential); Postsynapse (Probable); Presynapse (Probable)

Isoforms & protein length

Isoform 1 (main): 1630 aa; Isoform 1 (main): 1630 aa; Isoform 2 : 1549 aa; Isoform 3 : 1655 aa

Known functions

Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity. Interacts (via PDZ domains) with VANGL2. Interacts with CTNNB1 and MAPK12 (By similarity). Interacts with UBE3A and HPV E6. Interacts with PAK1 and PAK2. Interacts with ARHGEF7 and GIT1; interacts directly with ARHGEF7. Interacts (via PDZ domains) with LPP and TRIP6; the interaction is direct. Interacts (via PDZ domains) with TJP2. Interacts (via PDZ domains) with APC; may mediate APC targeting to adherens junctions of epithelial cells. Interacts (via PDZ domains) with TSHR; regulates TSHR trafficking and function. Interacts (via PDZ domains 1 and 3) with MCC. Interacts (via fourth PDZ domain) with tick-borne encephalitis virus NS5 protein; this interaction inhibits SCRIB and downstream STAT1 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway. Interacts with DLG5 (PubMed:28169360). Interacts with STK4/MST1 and LATS1 in the presence of DLG5 (PubMed:28169360). (Microbial infection) Interacts with tick-borne encephalitis virus RNA-directed RNA polymerase NS5; this interaction targets viral NS5 to the cell membrane periphery and nucleus, thereby allowing efficient host nuclear STAT1 inhibition. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions. Scaffold protein involved in different aspects of polarized cell differentiation regulating epithelial and neuronal morphogenesis and T-cell polarization (PubMed:15182672, PubMed:16344308, PubMed:19041750, PubMed:18716323, PubMed:18641685, PubMed:16965391, PubMed:27380321). Via its interaction with CRTAM, required for the late phase polarization of a subset of CD4+ T-cells, which in turn regulates TCR-mediated proliferation and IFNG and IL22 production (By similarity). Most probably functions in the establishment of apico-basal cell polarity (PubMed:16344308, PubMed:19041750). May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium (PubMed:16965391, PubMed:19041750). May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling (PubMed:18716323, PubMed:18641685). May play a role in exocytosis and in the targeting of synaptic vesicles to synapses (PubMed:15182672). Functions as an activator of Rac GTPase activity. Interacts with UBE3A (PubMed:11027293). Interacts with PAK1 and PAK2 (PubMed:18716323). Interacts (via PDZ domains) with VANGL2 (By similarity). Interacts (via PDZ domains) with LPP and TRIP6; the interaction is direct (PubMed:15649318, PubMed:16137684). Interacts (via PDZ domains) with TJP2 (PubMed:15975580). Interacts (via PDZ domains) with APC; may mediate APC targeting to adherens junctions of epithelial cells (PubMed:16611247). Interacts (via PDZ domains) with TSHR; regulates TSHR trafficking and function (PubMed:15775968). Interacts with ARHGEF7 and GIT1; interacts directly with ARHGEF7 (PubMed:15182672). Interacts with CTNNB1 (By similarity). Interacts with MAPK12 (By similarity). Interacts (via PDZ domains 1 and 3) with MCC (PubMed:19555689, PubMed:22480440). Interacts with DLG5 (PubMed:28169360). Interacts with STK4/MST1 and LATS1 in the presence of DLG5 (PubMed:28169360). Interacts (via PDZ domain 3) with CRTAM (via PDZ-binding motif); the interaction promotes CRTAM and SCRIB polarization in a subset of CD4+ T-cells (By similarity). (Microbial infection) Interacts (via fourth PDZ domain) with tick-borne encephalitis virus RNA-directed RNA polymerase NS5; this interaction targets viral NS5 to the cell membrane periphery and nucleus and prevents STAT1 phosphorylation, and thus, the activation of the JAK-STAT signaling pathway (PubMed:18042258). Interacts with HPV E6 (PubMed:11027293). Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. In a subset of CD4+ T-cells, colocalizes with CRTAM at the immunological synapse during the late phase of T-cell activation (By similarity). Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity. Interacts (via PDZ domains) with VANGL2. Interacts with CTNNB1 and MAPK12 (By similarity). Interacts with UBE3A and HPV E6. Interacts with PAK1 and PAK2. Interacts with ARHGEF7 and GIT1; interacts directly with ARHGEF7. Interacts (via PDZ domains) with LPP and TRIP6; the interaction is direct. Interacts (via PDZ domains) with TJP2. Interacts (via PDZ domains) with APC; may mediate APC targeting to adherens junctions of epithelial cells. Interacts (via PDZ domains) with TSHR; regulates TSHR trafficking and function. Interacts (via PDZ domains 1 and 3) with MCC. Interacts (via fourth PDZ domain) with tick-borne encephalitis virus NS5 protein; this interaction inhibits SCRIB and downstream STAT1 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway. Interacts with DLG5 (PubMed:28169360). Interacts with STK4/MST1 and LATS1 in the presence of DLG5 (PubMed:28169360). (Microbial infection) Interacts with tick-borne encephalitis virus RNA-directed RNA polymerase NS5; this interaction targets viral NS5 to the cell membrane periphery and nucleus, thereby allowing efficient host nuclear STAT1 inhibition. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.

Cross-references

Source	Identifiers
UniProt gene names	SCRIB, CRIB1, KIAA0147, LAP4, SCRB1, VARTUL
UniProt secondary AC	Q6P496, Q7Z5D1, Q8WWV8, Q96C69, Q96GG1
Refseq protein ID	NP_056171.3, NP_874365.3
IPI ID	IPI00410666, IPI00425560, IPI00425562, IPI00425566, IPI00376118, IPI00290245, IPI00062541

Source	Identifiers
Ensembl protein	ENSP00000322938, ENSP00000349486, ENSP00000366756, ENSP00000456859, ENSP00000456561, ENSP00000455629
Ensembl gene	ENSG00000180900

Enzymes

PAT/APT	Enzyme	Experiments
PAT	ZDHC7_HUMAN	SPalmE:796 Chen B et al. (2016) SPalmE:798 Chen B et al. (2016)
PAT	ZDHC3_HUMAN	SPalmE:798 Chen B et al. (2016)
APT	LYPA2_HUMAN	SPalmE:1061 Hernandez JL et al. (2017)

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

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UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Sites	Predicted
F6RIN9	F6RIN9_MOUSE	Ensembl	M. musculus	Scrib	Protein scribble homolog	0/23	0	0	0	No
Q7KRY7	SCRIB_DROME	Ensembl, InParanoid	D. melanogaster	scrib, smi, vart, CG5462	Protein lap4	1/1	2	2	0	Yes
Q8W4Q3	PIRL3_ARATH	MetaPhors	A. thaliana	PIRL3, At1g12970, F13K23.23	Plant intracellular Ras-group-related LRR protein 3	0/1	0	0	0	No
Q80U72	SCRIB_MOUSE	OMA, Ensembl, InParanoid	M. musculus	Scrib, Kiaa0147, Lap4, Scrib1	Protein scribble homolog, Scribble	10/23	0	3	1	Yes
F1PKY2	F1PKY2_CANLF	OMA, Ensembl, InParanoid	C. familiaris	SCRIB	Scribbled planar cell polarity protein	1/1	0	2	0	Yes
F1N0Y1	F1N0Y1_BOVIN	OMA, Ensembl, InParanoid	B. taurus	SCRIB	Scribble planar cell polarity protein	0/2	0	0	0	Yes
D3ZWS0	D3ZWS0_RAT	OMA, Ensembl, InParanoid	R. norvegicus	Scrib, RGD1565055_predicted, rCG_60094	Scribble planar cell polarity protein	1/3	0	1	0	Yes

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:796	Chen B et al.	2016	Homo sapiens	HEK293-A	Total cell lysate	15-HDYA labeling, 2BP inhibition, Co-IP of PAT and substrate, Depletion of PAT by siRNA, MS, NH2OH cleavage of thioester bond, PAT overexpression, PAT point mutation, Point mutation
SPalmE:797	Chen B et al.	2016	Homo sapiens	MDCK	Total cell lysate	IF localization, Point mutation
SPalmE:798	Chen B et al.	2016	Homo sapiens	MCF-10A	Total cell lysate	Depletion of PATs by SgRNA, IF localization, Point mutation
SPalmE:1061	Hernandez JL et al.	2017	Homo sapiens	MCF-10A	Total cell lysate	, ABE, Click chemistry, NH2OH cleavage of thioester bond, Thioesterases overexpression
SPalmE:1167	Won SJ et al.	2018	Homo sapiens	HAP1	Total membrane	, 2BP inhibition, ARAC
SPalmE:1141	Won SJ et al.	2018	Homo sapiens	HEK293T	Total membrane	, 2BP inhibition, ARAC

Sites (from validation or large-scale experiments)

Isoforms	Position	Probability	Studies	Techniques
Q14160	4	NA	SPalmE:578 Yang W et al. (2010)* SPalmE:798 Chen B et al. (2016) SPalmE:797 Chen B et al. (2016) SPalmE:796 Chen B et al. (2016)	15-HDYA labeling, 2BP inhibition, ABE, Co-IP of PAT and substrate, Depletion of PAT by siRNA, Depletion of PATs by SgRNA, IF localization, MS, NH2OH cleavage of thioester bond, PAT overexpression, PAT point mutation, Point mutation
Q14160	10	NA	SPalmE:796 Chen B et al. (2016) SPalmE:797 Chen B et al. (2016) SPalmE:798 Chen B et al. (2016)	15-HDYA labeling, 2BP inhibition, Co-IP of PAT and substrate, Depletion of PAT by siRNA, Depletion of PATs by SgRNA, IF localization, MS, NH2OH cleavage of thioester bond, PAT overexpression, PAT point mutation, Point mutation

Cysteine summary and predictions

Pos in alignment	4	10	22	104	224	279	288	334	413	496	498	864	1082	1102
Subcell. location	U	U	U	U	U	U	U	U	U	U	U	U	U	U
Disulfide
PSIPRED v.4.02 prediction
UniProt secondary structure
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred
Pos in isoform 2 CSS-Palm 4.0 PalmPred				Cys²³ HC	Cys¹⁴³	Cys¹⁹⁸	Cys²⁰⁷	Cys²⁵³	Cys³³²	Cys⁴¹⁵	Cys⁴¹⁷	Cys⁷⁸³	Cys¹⁰⁰¹	Cys¹⁰²¹
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys⁴ MC	Cys¹⁰ HC	Cys²² HC	Cys¹⁰⁴ LC	Cys²²⁴	Cys²⁷⁹	Cys²⁸⁸	Cys³³⁴	Cys⁴¹³	Cys⁴⁹⁶	Cys⁴⁹⁸	Cys⁸⁶⁴	Cys¹⁰⁸²	Cys¹¹⁰²

Pos in alignment	1189	1297	1637
Subcell. location	U	U	U
Disulfide
PSIPRED v.4.02 prediction
UniProt secondary structure
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys¹¹⁰⁸	Cys¹²¹⁶ LC	Cys¹⁵³¹
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys¹¹⁸⁹	Cys¹²⁹⁷ LC	Cys¹⁶³⁷

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

Tip: Keep CTRL key pressed down while clicking on column headers to do multiple sorting. This applies to any sortable table on this site.

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	Ortholog	in LC [D3ZWS0]	HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	Found	in HC	HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	Found	in HC	HC: 162 hits LC: 218 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	Found	in HC	HC: 102 hits LC: 73 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	Ortholog	in HC [SCRIB]	HC HC: 352 hits LC LC: 8 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	Ortholog	in HC [SCRIB]	HC HC: 254 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	x		HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	Ortholog	in List [SCRIB]	List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	Ortholog	in List [SCRIB]	List: 436 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	Found	in List	Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	Found	in List	List: 354 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Found	in List	List: 1348 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	Found	in List	List: 354 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	Found	in List	List: 226 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	Found	in List	List: 274 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	Found	in List	List: 1353 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	x		HC: 1079 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	Ortholog	in List [SCRIB]	List: 865 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Found	in HC	HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	Found	in HC	HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Ortholog	in HC [SCRIB]	HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in LC [SCRIB]	HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in LC [SCRIB]	HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	Found	in List	List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	Found	in List	List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	Found	in List	List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in LPS treatment [SCRIB]	LPS treatment: 646 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in List [SCRIB]	List: 646 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Ortholog	in HC [SCRIB]	HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	Ortholog	in List [SCRIB]	List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	Ortholog	in List [SCRIB]	List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	Found	in List	List: 2929 hits
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	Found	in List	List: 590 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [SCRIB]	List: 370 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 600 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	Found	in	Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	Found	in List	List: 185 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1869 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1093 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Found	in List	List: 687 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Found	in List	List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Found	in List	List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [SCRIB]	List: 199 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 399 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1040 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [SCRIB]	HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [F1PKY2]	List: 464 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [SCRIB]	HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [SCRIB]	HC: 4107 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1597 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1378 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [SCRIB]	HC: 3870 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	1	25.0	D3ZWS0_RAT
H. sapiens	22	38	26	68.4	NA
M. musculus	23	37	17	45.9	SCRIB_MOUSE
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
A. thaliana	1	1	0	0.0	NA
S. pombe	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	2	100.0	SCRIB_DROME
C. familiaris	1	1	1	100.0	F1PKY2_CANLF

References

Authors	Year	Reference title	PMID
Kang R et al.	2008	Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.	19092927
Ren W et al.	2013	Proteomic analysis of protein palmitoylation in adipocytes.	23599907
Marin EP et al.	2012	Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases.	22496122
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
Martin BR et al.	2011	Global profiling of dynamic protein palmitoylation.	22056678
Dowal L et al.	2011	Proteomic analysis of palmitoylated platelet proteins.	21813449
Merrick BA et al.	2011	Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3.	21785166
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
Forrester MT et al.	2011	Site-specific analysis of protein S-acylation by resin-assisted capture.	21044946
Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
Yang W et al.	2010	Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes.	19801377
Martin BR et al.	2009	Large-scale profiling of protein palmitoylation in mammalian cells.	19137006
Roth AF et al.	2006	Global analysis of protein palmitoylation in yeast.	16751107
Hemsley PA et al.	2013	A proteomic approach identifies many novel palmitoylated proteins in Arabidopsis.	23252521
Ivaldi C et al.	2012	Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.	22615937
Wan J et al.	2013	Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease.	24211138
Zhang MM et al.	2013	Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.	23843742
Jones ML et al.	2012	Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis.	22901544
Wei X et al.	2014	Insulin-regulated protein palmitoylation impacts endothelial cell function.	24357059
Serwa RA et al.	2015	Systems Analysis of Protein Fatty Acylation in Herpes Simplex Virus-Infected Cells Using Chemical Proteomics.	26256475
Morrison E et al.	2015	Quantitative analysis of the human T cell palmitome.	26111759
Chesarino NM et al.	2014	Chemoproteomics reveals Toll-like receptor fatty acylation.	25371237
Santiago-Tirado FH et al.	2015	A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.	25970403
Emmer BT et al.	2011	Global analysis of protein palmitoylation in African trypanosomes.	21193548
Colquhoun DR et al.	2015	Bioorthogonal mimetics of palmitoyl-CoA and myristoyl-CoA and their subsequent isolation by click chemistry and characterization by mass spectrometry reveal novel acylated host-proteins modified by HIV-1 infection.	25914232
Foe IT et al.	2015	Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.	26468752
Gould NS et al.	2015	Site-Specific Proteomic Mapping Identifies Selectively Modified Regulatory Cysteine Residues in Functionally Distinct Protein Networks.	26165157
Peng T et al.	2015	Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.	25575299
Pinner AL et al.	2016	Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia.	26876311
Hernandez JL et al.	2016	Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.	27030425
Fang C et al.	2016	Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody.	26865113
Caballero MC et al.	2016	Identification of new palmitoylated proteins in Toxoplasma gondii.	26825284
Chen B et al.	2016	ZDHHC7-mediated S-palmitoylation of Scribble regulates cell polarity.	27380321
Upadhyay S et al.	2016	Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation.	27879337
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
Sobocińska J et al.	2018	Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.	29217618
Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Thinon E et al.	2016	Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.	27350074
Hernandez JL et al.	2017	APT2 Inhibition Restores Scribble Localization and S-Palmitoylation in Snail-Transformed Cells.	28065656
Thinon E et al.	2018	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.	29575903
Zhang X et al.	2018	Ultradeep Palmitoylomics Enabled by Dithiodipyridine-Functionalized Magnetic Nanoparticles.	29660268
Won SJ et al.	2018	Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.	29733200
Dumoulin A et al.	2018	S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.	30319353
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Wang J et al.	2019	DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane.	30605677
Strassburger K et al.	2019	Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.	30735487
Zhou B et al.	2019	Low-background Acyl-biotinyl Exchange Largely Eliminates the Co-isolation of Non-S-acylated Proteins and Enables Deep S-acylproteomic Analysis.	31251020
Zaręba-Kozioł M et al.	2019	Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins.	31311849
Wegleiter T et al.	2019	Palmitoylation of BMPR1a regulates neural stem cell fate.	31772009
Morrison E et al.	2020	Dynamic palmitoylation events following T-cell receptor signaling.	32651440
Mariscal J et al.	2020	Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles.	32944167
Gorenberg EL et al.	2022	Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.	35358180
Uzbekova S et al.	2021	Protein Palmitoylation in Bovine Ovarian Follicle.	34769186
Miles MR et al.	2021	Global identification of S-palmitoylated proteins and detection of palmitoylating (DHHC) enzymes in heart.	33636221
Pradhan AJ et al.	2021	Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.	33848465
Ripamonti M et al.	2021	Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions.	33782527
Zaręba-Kozioł M et al.	2021	S-Palmitoylation of Synaptic Proteins as a Novel Mechanism Underlying Sex-Dependent Differences in Neuronal Plasticity.	34200797
Ziemlińska E et al.	2021	Palm Oil-Rich Diet Affects Murine Liver Proteome and <i>S</i>-Palmitoylome.	34884899
Gao J et al.	2022	Proteome-wide identification of palmitoylated proteins in mouse testis.	35477839

Q14160 SCRIB_HUMAN