SwissPalm

Protein general information from UniProt

Protein names

Palmitoyltransferase ZDHHC20, EC 2.3.1.225; Acyltransferase ZDHHC20, EC 2.3.1.-; DHHC domain-containing cysteine-rich protein 20, DHHC20; Zinc finger DHHC domain-containing protein 20

Subcellular location

Membrane (Probable); Golgi apparatus membrane (Probable); Cell membrane (By similarity); Cytoplasm (Probable); Perinuclear region (Probable); Endoplasmic reticulum membrane; Endoplasmic reticulum-Golgi intermediate compartment membrane

Isoforms & protein length

Isoform 1 (main): 365 aa; Isoform 2 : 130 aa; Isoform 3 : 354 aa; Isoform 4 : 320 aa

Known functions

Catalyzes palmitoylation of Cys residues on target proteins (PubMed:27153536, PubMed:29326245). Catalyzes palmitoylation of Cys residues in the cytoplasmic C-terminus of EGFR, and modulates the duration of EGFR signaling by modulating palmitoylation-dependent EGFR internalization and degradation (PubMed:27153536). Has a preference for acyl-CoA with C16 fatty acid chains (PubMed:29326245). Can also utilize acyl-CoA with C14 and C18 fatty acid chains (PubMed:29326245). hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-hexadecanoyl-L-cysteinyl-[protein] (Microbial infection) Dominant palmitoyltransferase responsible for lipidation of SARS coronavirus-2/SARS-CoV-2 spike protein. Through a sequential action with ZDHHC9, rapidly and efficiently palmitoylates spike protein following its synthesis in the endoplasmic reticulum (ER). In the infected cell, promotes spike biogenesis by protecting it from premature ER degradation, increases half-life and controls the lipid organization of its immediate membrane environment. Once the virus has formed, spike palmitoylation controls fusion with the target cell. hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-hexadecanoyl-L-cysteinyl-[protein] L-cysteinyl-[protein] + tetradecanoyl-CoA = CoA + S-tetradecanoyl-L-cysteinyl-[protein] L-cysteinyl-[protein] + octadecanoyl-CoA = CoA + S-octadecanoyl-L-cysteinyl-[protein] Palmitoyltransferase that could catalyze the addition of palmitate onto various protein substrates (PubMed:27153536, PubMed:29326245, PubMed:33219126). Catalyzes palmitoylation of Cys residues in the cytoplasmic C-terminus of EGFR, and modulates the duration of EGFR signaling by modulating palmitoylation-dependent EGFR internalization and degradation (PubMed:27153536). Has a preference for acyl-CoA with C16 fatty acid chains (PubMed:29326245). Can also utilize acyl-CoA with C14 and C18 fatty acid chains (PubMed:29326245).

Cross-references

Source	Identifiers
UniProt gene names	ZDHHC20
UniProt secondary AC	A8MTV9, C9JG20, I6L9D4, Q2TB82, Q6NVU8
Refseq protein ID	XP_006719858.1, NP_694983.2, XP_005266385.1, NP_001273567.1
IPI ID	IPI00398974, IPI00655982, IPI00941781, IPI00872130, IPI00797413, IPI00329017, NP_694983.2

Source	Identifiers
Ensembl protein	ENSP00000313583, ENSP00000371905, ENSP00000383433, ENSP00000401232, ENSP00000396819
Ensembl gene	ENSG00000180776
CellPalmSeq	search-zdhhcs/zdhhc20

Protein acyltransferase activity (PAT)

Substrate UniProt ID	Substrate description	Substrate isoform AC (site position)	Experiments
FYN_HUMAN	Tyrosine-protein kinase Fyn, EC 2.7.10.2	P06241	SPalmE:148 Mill P et al. (2009)
ABCA1_HUMAN	Phospholipid-transporting ATPase ABCA1, EC 7.6.2.1	O95477	SPalmE:161 Singaraja RR et al. (2009)
EGFR_HUMAN	Epidermal growth factor receptor, EC 2.7.10.1	P00533 (1049,1146)	SPalmE:749 Runkle KB et al. (2016) SPalmE:750 Runkle KB et al. (2016)
IFM3_HUMAN	Interferon-induced transmembrane protein 3	Q01628	SPalmE:916 McMichael TM et al. (2017)
CTNB1_HUMAN	Catenin beta-1	P35222	SPalmE:1011 Stypulkowski E et al. (2018)
NUMB_HUMAN	Protein numb homolog, h-Numb	P49757	SPalmE:1011 Stypulkowski E et al. (2018)
MK10_HUMAN	Mitogen-activated protein kinase 10, MAP kinase 10, MAPK 10, EC 2.7.11.24	P53779	SPalmE:1115 Yang G et al. (2012)
TAT_HV1B1	Protein Tat	P69697 (31)	SPalmE:1147 Chopard C et al. (2018) SPalmE:1146 Chopard C et al. (2018)
HEMA_I34A0	Hemagglutinin [Cleaved into: Hemagglutinin HA1 chain; Hemagglutinin HA2 chain ]	P03459	SPalmE:1321 Gadalla MR et al. (2020) []
VAMP7_HUMAN	Vesicle-associated membrane protein 7, VAMP-7	P51809 (183)	SPalmE:1382 Morrison E et al. (2020) []
LAT_HUMAN	Linker for activation of T-cells family member 1	O43561	SPalmE:1382 Morrison E et al. (2020) []
ZDHC5_HUMAN	Palmitoyltransferase ZDHHC5, EC 2.3.1.225	Q9C0B5 (134,236,237)	SPalmE:1384 Plain F et al. (2020) []
MBLC2_HUMAN	Acyl-coenzyme A thioesterase MBLAC2, Acyl-CoA thioesterase MBLAC2, EC 3.1.2.2	Q68D91 (176,212,254)	SPalmE:1424 Malgapo MIP et al. (2021) []
BMR1A_HUMAN	Bone morphogenetic protein receptor type-1A, BMP type-1A receptor, BMPR-1A, EC 2.7.11.30	P36894 (173,175,180)	SPalmE:1431 Wegleiter T et al. (2019) []

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

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UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Sites	Predicted
Q5Y5T1	ZDH20_MOUSE	OrthoDB, OMA, Ensembl, InParanoid	M. musculus	Zdhhc20	Palmitoyltransferase ZDHHC20, EC 2.3.1.225	10/23	0	3	1	Yes
E2REV5	E2REV5_CANLF	Ensembl	C. familiaris	ZDHHC20	Palmitoyltransferase	0/1	0	0	0	Yes
Q94C49	ZDH18_ARATH	InParanoid	A. thaliana	At4g22750, T12H17.140, T12H17.2, PAT13	Probable protein S-acyltransferase 13, EC 2.3.1.225	1/1	0	1	0	Yes
E9PT74	E9PT74_RAT	OrthoDB, OMA, Ensembl, InParanoid, MetaPhors	R. norvegicus	Zdhhc20	Palmitoyltransferase	0/3	0	0	0	Yes
E2RKX3	E2RKX3_CANLF	OrthoDB, OMA, Ensembl, InParanoid	C. familiaris	ZDHHC20	Palmitoyltransferase	0/1	0	0	0	Yes
F1MPF2	F1MPF2_BOVIN	OrthoDB	B. taurus	ZDHHC2	Palmitoyltransferase	0/2	0	0	0	Yes
A1Z833	A1Z833_DROME	OrthoDB, OMA, Ensembl, InParanoid, MetaPhors	D. melanogaster	Dmel\CG1407, CG1407, Dmel_CG1407	Palmitoyltransferase	0/1	0	0	0	Yes
Q03289	PFA5_YEAST	OMA	S. cerevisiae S288c	PFA5, YDR459C	Palmitoyltransferase PFA5, EC 2.3.1.225	0/1	0	0	0	No
Q5M757	ZDH15_ARATH	OMA, InParanoid, MetaPhors	A. thaliana	At4g00840, A_TM018A10.8, T18A10.14, PAT12	Probable protein S-acyltransferase 12, EC 2.3.1.225	0/1	0	0	0	Yes
Q8VYP5	ZDH14_ARATH	OMA, InParanoid	A. thaliana	At3g60800, T4C21_210, PAT14	Probable protein S-acyltransferase 14, EC 2.3.1.225	0/1	1	0	1	Yes
Q0VC89	ZDH20_BOVIN	OMA, Ensembl, OrthoDB, InParanoid, MetaPhors	B. taurus	ZDHHC20	Palmitoyltransferase ZDHHC20, EC 2.3.1.225	0/2	0	0	0	Yes
A0JPI6	A0JPI6_RAT	Ensembl	R. norvegicus	Zdhhc20	Palmitoyltransferase	0/3	0	0	0	Yes
A1ZAR2	A1ZAR2_DROME	Ensembl, MetaPhors	D. melanogaster	Dmel\CG17287, CG17287, Dmel_CG17287	Palmitoyltransferase	0/1	0	0	0	Yes
E2QCN5	E2QCN5_DROME	Ensembl, InParanoid	D. melanogaster	Dmel\CG1407, CG1407, Dmel_CG1407	Palmitoyltransferase	0/1	0	0	0	Yes
Q7K2V5	Q7K2V5_DROME	Ensembl, InParanoid	D. melanogaster	Dmel\CG1407, CG1407, Dmel_CG1407	Palmitoyltransferase	0/1	0	0	0	Yes
Q960T3	Q960T3_DROME	InParanoid	D. melanogaster	CG1407	Palmitoyltransferase	0/1	0	0	0	Yes
O14345	PFA3_SCHPO	InParanoid	S. pombe	pfa3, SPBC2F12.15c	Palmitoyltransferase pfa3	0/1	0	0	0	Yes
Q8I3I3	Q8I3I3_PLAF7	InParanoid, OMA	P. falciparum	PF3D7_0528400	Palmitoyltransferase	0/1	0	0	0	Yes
Q8IJC5	Q8IJC5_PLAF7	InParanoid	P. falciparum	PF10_0273	Palmitoyltransferase	0/1	0	0	0	Yes
P42836	PFA3_YEAST	InParanoid	S. cerevisiae S288c	PFA3, YNL326C, N0325	Palmitoyltransferase PFA3, EC 2.3.1.225	0/1	0	0	0	No

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:148	Mill P et al.	2009	Homo sapiens	HEK293	NA	PAT overexpression, Point mutation, [3H]palmitic acid labeling
SPalmE:161	Singaraja RR et al.	2009	Homo sapiens	Flp-In-293	Total cell lysate	PAT overexpression, [3H]palmitic acid labeling
SPalmE:749	Runkle KB et al.	2016	Homo sapiens	MDA-MB-231	Total cell lysate	2BP inhibition, ABE, Depletion of PAT by shRNA, IF localization, NH2OH cleavage of thioester bond, PAT localization by IF
SPalmE:750	Runkle KB et al.	2016	Homo sapiens	HEK293T	Total cell lysate	ABE, MS, NH2OH cleavage of thioester bond, PAT overexpression, Point mutation, Substrate truncation
SPalmE:916	McMichael TM et al.	2017	Homo sapiens	HAP1	Total cell lysate	Alk16 Labeling, Depletion of PAT by siRNA
SPalmE:1011	Stypulkowski E et al.	2018	Homo sapiens	MDA-MB-231	Plasma Membrane	, , ABE, Depletion of PAT by shRNA, IF localization, NH2OH cleavage of thioester bond, Palmostatin B inhibition, Substrate localization by fluorescence tag
SPalmE:1115	Yang G et al.	2012	Homo sapiens	HEK293	Total cell lysate	ABE, PAT overexpression
SPalmE:1146	Chopard C et al.	2018	Rattus norvegicus	PC12	Plasma Membrane	2BP inhibition, ABE, Click chemistry, IF localization, NH2OH cleavage of thioester bond, Point mutation
SPalmE:1147	Chopard C et al.	2018	Homo sapiens	HEK293T	Total cell lysate	ARAC, Co-IP of PAT and substrate, NH2OH cleavage of thioester bond, PAT overexpression, Substrate overexpression
SPalmE:1321	Gadalla MR et al.	2019	Homo sapiens	HAP1	Viral fraction	ARAC, Depletion of PAT by siRNA, NH2OH cleavage of thioester bond, [3H]palmitic acid labeling
SPalmE:1382	Morrison E et al.	2020	Spodoptera frugiperda	Sf9	NA	15-HDYA labeling, OPPA, PAT overexpression, PAT point mutation, Point mutation, Substrate overexpression
SPalmE:1384	Plain F et al.	2020	Homo sapiens	HEK293	Total cell lysate	APE, ARAC, NH2OH cleavage of thioester bond, Point mutation
SPalmE:1424	Malgapo MIP et al.	2020	Homo sapiens	HEK293	Total cell lysate	Click chemistry, IF localization
SPalmE:1431	Wegleiter T et al.	2019	Homo sapiens	Neural stem cell	Total cell lysate	ABE, APE, MS, Substrate localization by fluorescence tag

Cysteine summary and predictions

Pos in alignment	9	10	38	128	131	142	145	148	156	162	182	232	263	290
Subcell. location	C	C	E	C	C	C	C	C	C	C	T	C	C	C
Disulfide	no	no	no	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	helix	helix	helix	coil	coil	coil	coil	strand	coil	strand	helix	helix	helix	coil
UniProt secondary structure	helix	helix	helix	NA	coil	strand	coil	strand	strand	strand	helix	helix	helix	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁹ HC	Cys¹⁰ HC	Cys³⁸	Cys¹²⁸	Cys¹³¹	Cys¹⁴²	Cys¹⁴⁵	Cys¹⁴⁸ MC	Cys¹⁵⁶	Cys¹⁶² MC	Cys¹⁸² LC	Cys²³² MC	Cys²⁶³	Cys²⁹⁰
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys⁹ HC	Cys¹⁰ HC	Cys³⁸
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys⁹ HC	Cys¹⁰ HC	Cys³⁸	Cys¹²⁸	Cys¹³¹	Cys¹⁴²	Cys¹⁴⁵	Cys¹⁴⁸ MC	Cys¹⁵⁶	Cys¹⁶² MC	Cys¹⁸² LC	Cys²³² MC	Cys²⁶³	Cys²⁹⁰
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys⁹ HC	Cys¹⁰ HC	Cys³⁸	Cys¹²⁸	Cys¹³¹	Cys¹⁴²	Cys¹⁴⁵	Cys¹⁴⁸ MC	Cys¹⁵⁶	Cys¹⁶² MC	Cys¹⁸² LC	Cys²³² MC	Cys²⁶³	Cys²⁹⁰

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

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ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	Found	in HC	HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	Found	in HC	HC: 162 hits LC: 218 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	Found	in HC	HC: 102 hits LC: 73 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	Ortholog	in HC [ZDH20]	HC HC: 352 hits LC LC: 8 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	Ortholog	in HC [ZDH20]	HC HC: 254 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	Ortholog	in LC [ZDH18]	HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	Ortholog	in List [ZDH20]	List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	Ortholog	in List [ZDH20]	List: 436 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	x		Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	Ortholog	in List [B4DRN8]	List: 354 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Found	in List	List: 1348 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	Ortholog	in List [B4DRN8]	List: 354 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	x		List: 226 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	Found	in List	List: 274 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	Ortholog	in MC [ZDH20]	HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	x		List: 1353 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	Ortholog	in HC [ZDH20]	HC: 1079 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		List: 865 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Ortholog	in HC [ZDH20]	HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in MC [ZDH20]	HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in HC [ZDH20]	HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	x		List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	x		List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in LPS treatment [ZDH20]	LPS treatment: 646 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in List [ZDH20]	List: 646 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Ortholog	in HC [ZDH20]	HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	Found	in List	List: 2929 hits
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 590 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [ZDH20]	List: 370 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 600 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		List: 185 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1869 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1093 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 687 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Found	in List	List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Found	in List	List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 199 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 399 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1040 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 464 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in MC [ZDH20]	HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [ZDH20]	HC: 4107 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1597 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1378 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [ZDH20]	HC: 3870 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	0	0.0	NA
H. sapiens	22	38	14	36.8	B4DRN8_HUMAN
M. musculus	23	37	16	43.2	ZDH20_MOUSE
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
A. thaliana	1	1	1	100.0	ZDH18_ARATH
S. pombe	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	0	0.0	A1Z833_DROME , E2QCN5_DROME
C. familiaris	1	1	0	0.0	NA

References

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Ren W et al.	2013	Proteomic analysis of protein palmitoylation in adipocytes.	23599907
Marin EP et al.	2012	Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases.	22496122
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
Martin BR et al.	2011	Global profiling of dynamic protein palmitoylation.	22056678
Dowal L et al.	2011	Proteomic analysis of palmitoylated platelet proteins.	21813449
Merrick BA et al.	2011	Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3.	21785166
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
Forrester MT et al.	2011	Site-specific analysis of protein S-acylation by resin-assisted capture.	21044946
Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
Yang W et al.	2010	Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes.	19801377
Martin BR et al.	2009	Large-scale profiling of protein palmitoylation in mammalian cells.	19137006
Roth AF et al.	2006	Global analysis of protein palmitoylation in yeast.	16751107
Hemsley PA et al.	2013	A proteomic approach identifies many novel palmitoylated proteins in Arabidopsis.	23252521
Singaraja RR et al.	2009	Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function.	19556522
Ivaldi C et al.	2012	Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.	22615937
Wan J et al.	2013	Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease.	24211138
Mill P et al.	2009	Palmitoylation regulates epidermal homeostasis and hair follicle differentiation.	19956733
Zhang MM et al.	2013	Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.	23843742
Jones ML et al.	2012	Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis.	22901544
Wei X et al.	2014	Insulin-regulated protein palmitoylation impacts endothelial cell function.	24357059
Yang G et al.	2012	Isoform-specific palmitoylation of JNK regulates axonal development.	21941371
Serwa RA et al.	2015	Systems Analysis of Protein Fatty Acylation in Herpes Simplex Virus-Infected Cells Using Chemical Proteomics.	26256475
Morrison E et al.	2015	Quantitative analysis of the human T cell palmitome.	26111759
Chesarino NM et al.	2014	Chemoproteomics reveals Toll-like receptor fatty acylation.	25371237
Santiago-Tirado FH et al.	2015	A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.	25970403
Emmer BT et al.	2011	Global analysis of protein palmitoylation in African trypanosomes.	21193548
Colquhoun DR et al.	2015	Bioorthogonal mimetics of palmitoyl-CoA and myristoyl-CoA and their subsequent isolation by click chemistry and characterization by mass spectrometry reveal novel acylated host-proteins modified by HIV-1 infection.	25914232
Foe IT et al.	2015	Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.	26468752
Gould NS et al.	2015	Site-Specific Proteomic Mapping Identifies Selectively Modified Regulatory Cysteine Residues in Functionally Distinct Protein Networks.	26165157
Peng T et al.	2015	Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.	25575299
Pinner AL et al.	2016	Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia.	26876311
Hernandez JL et al.	2016	Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.	27030425
Fang C et al.	2016	Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody.	26865113
Caballero MC et al.	2016	Identification of new palmitoylated proteins in Toxoplasma gondii.	26825284
Runkle KB et al.	2016	Inhibition of DHHC20-Mediated EGFR Palmitoylation Creates a Dependence on EGFR Signaling.	27153536
Upadhyay S et al.	2016	Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation.	27879337
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
McMichael TM et al.	2017	The palmitoyltransferase ZDHHC20 enhances interferon-induced transmembrane protein 3 (IFITM3) palmitoylation and antiviral activity.	29079573
Sobocińska J et al.	2018	Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.	29217618
Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Thinon E et al.	2016	Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.	27350074
Stypulkowski E et al.	2018	The depalmitoylase APT1 directs the asymmetric partitioning of Notch and Wnt signaling during cell division.	29295957
Thinon E et al.	2018	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.	29575903
Zhang X et al.	2018	Ultradeep Palmitoylomics Enabled by Dithiodipyridine-Functionalized Magnetic Nanoparticles.	29660268
Chopard C et al.	2018	Cyclophilin A enables specific HIV-1 Tat palmitoylation and accumulation in uninfected cells.	29884859
Won SJ et al.	2018	Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.	29733200
Dumoulin A et al.	2018	S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.	30319353
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Wang J et al.	2019	DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane.	30605677
Strassburger K et al.	2019	Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.	30735487
Zhou B et al.	2019	Low-background Acyl-biotinyl Exchange Largely Eliminates the Co-isolation of Non-S-acylated Proteins and Enables Deep S-acylproteomic Analysis.	31251020
Zaręba-Kozioł M et al.	2019	Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins.	31311849
Wegleiter T et al.	2019	Palmitoylation of BMPR1a regulates neural stem cell fate.	31772009
Gadalla MR et al.	2019	Hemagglutinin of Influenza A, but not of Influenza B and C viruses is acylated by ZDHHC2, 8, 15 and 20.	31872235
Plain F et al.	2020	Control of protein palmitoylation by regulating substrate recruitment to a zDHHC-protein acyltransferase.	32737405
Morrison E et al.	2020	Dynamic palmitoylation events following T-cell receptor signaling.	32651440
Mariscal J et al.	2020	Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles.	32944167
Malgapo MIP et al.	2020	Metallo-β-Lactamase Domain-Containing Protein 2 (MBLAC2) is S-palmitoylated and exhibits acyl-CoA hydrolase activity.	33219126
Gorenberg EL et al.	2022	Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.	35358180
Uzbekova S et al.	2021	Protein Palmitoylation in Bovine Ovarian Follicle.	34769186
Miles MR et al.	2021	Global identification of S-palmitoylated proteins and detection of palmitoylating (DHHC) enzymes in heart.	33636221
Pradhan AJ et al.	2021	Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.	33848465
Ripamonti M et al.	2021	Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions.	33782527
Zaręba-Kozioł M et al.	2021	S-Palmitoylation of Synaptic Proteins as a Novel Mechanism Underlying Sex-Dependent Differences in Neuronal Plasticity.	34200797
Ziemlińska E et al.	2021	Palm Oil-Rich Diet Affects Murine Liver Proteome and <i>S</i>-Palmitoylome.	34884899
Gao J et al.	2022	Proteome-wide identification of palmitoylated proteins in mouse testis.	35477839

Q5W0Z9 ZDH20_HUMAN