SwissPalm

Q6R0H7 GNAS1_MOUSE

General info Cross-references Sequences Orthologs Ortholog sequences Validation experiments Sites Predictions Palmitoyl-proteomes References

Palmitoyl-proteomes

6/23 articles for mouse
6 orthologs

Palmitoyl-proteome experiments

Validation experiments

1 experiment
5 orthologs

Validation experiments

Curated sites & Enzymes

0 site from validation experiments^*
2 sites from palmitoyl-proteomes^* Cys¹¹⁰⁴, Cys⁹¹³
No PAT known
No APT known
^*In main isoform only

Sites

Predictions

Predicted

3 cysteines in main isoform

Predictions

Protein general information from UniProt

Protein names

Guanine nucleotide-binding protein G; s subunit alpha isoforms XLas; Adenylate cyclase-stimulating G alpha protein; Extra large alphas protein, XLalphas

Subcellular location

Apical cell membrane; Cell membrane (By similarity)

Isoforms & protein length

Isoform 1 (main): 1133 aa; Isoform 2 : 876 aa; Isoform 3 : 827 aa; Isoform 4 : 1119 aa

Known functions

Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP. GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. XLas isoforms interact with the same set of receptors as Gnas isoforms. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts through its N-terminal region with ALEX which is produced from the same locus in a different open reading frame. This interaction may inhibit its adenylyl cyclase-stimulating activity (By similarity). Interacts with MAGED2. Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP. GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. XLas isoforms interact with the same set of receptors as Gnas isoforms. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts through its N-terminal region with ALEX which is produced from the same locus in a different open reading frame. This interaction may inhibit its adenylyl cyclase-stimulating activity (By similarity). Interacts with MAGED2. Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP. GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. XLas isoforms interact with the same set of receptors as Gnas isoforms. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts through its N-terminal region with ALEX which is produced from the same locus in a different open reading frame. This interaction may inhibit its adenylyl cyclase-stimulating activity (By similarity). Interacts with MAGED2.

Cross-references

Source	Identifiers
UniProt gene names	Gnas1, Gnas
UniProt secondary AC	Q9Z1N8, Q9JJX0, Q6R2J5, Q6R0H5, Q6R0H4, A2A608, A2A607
Refseq protein ID	NP_963911.1, NP_034439.2, NP_001070975.1, NP_001297014.1
IPI ID	IPI00130294, IPI00406802, IPI00420409, IPI00471140, IPI01008606, IPI00798513, IPI00622913, IPI00469793, IPI00416906

Source	Identifiers
Ensembl protein	ENSMUSP00000085185, ENSMUSP00000085184, ENSMUSP00000079341, ENSMUSP00000140174
Ensembl gene	ENSMUSG00000027523
MGI ID	MGI:95777

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

Select all Deselect all

UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Sites	Predicted
P84996	ALEX_HUMAN	Ensembl	H. sapiens	GNAS1, GNAS	Protein ALEX	11/22	0	3	0	Yes
Q5JWF2	GNAS1_HUMAN	MetaPhors, Ensembl, InParanoid	H. sapiens	GNAS1, GNAS	Guanine nucleotide-binding protein G	4/22	4	3	0	Yes
P63092	GNAS2_HUMAN	Ensembl	H. sapiens	GSP, GNAS1, GNAS	Guanine nucleotide-binding protein G	8/22	3	4	5	Yes
O95467	GNAS3_HUMAN	Ensembl	H. sapiens	GNAS1, GNAS	Neuroendocrine secretory protein 55, NESP55 [Cleaved into: LHAL tetrapeptide; GPIPIRRH peptide ]	0/22	0	0	0	Yes
F1N0T0	F1N0T0_BOVIN	MetaPhors, Ensembl	B. taurus		Uncharacterized protein	0/2	0	0	0	No
P20354	GNAS_DROME	MetaPhors, Ensembl	D. melanogaster	Galphas, CG2835, G-sa60A, G-salpha60A	G protein alpha s subunit	1/1	0	2	0	Yes
P04896	GNAS2_BOVIN	MetaPhors	B. taurus	GNAS1, GNAS	Guanine nucleotide-binding protein G	2/2	4	2	1	Yes
C6KIE6	XLG2_ARATH	MetaPhors	A. thaliana	F10M10.160, At4g34390, XLG2	Extra-large guanine nucleotide-binding protein 2	0/1	0	0	0	No
Q9C516	XLG3_ARATH	MetaPhors	A. thaliana	T12O21.16, F5M6.7, At1g31930, XLG3	Extra-large guanine nucleotide-binding protein 3	0/1	0	0	0	No
P18064	GPA1_ARATH	MetaPhors	A. thaliana	T1D16.6, At2g26300, GPA1	Guanine nucleotide-binding protein alpha-1 subunit, GP-alpha-1	1/1	1	1	0	Yes
O80462	XLG1_ARATH	MetaPhors	A. thaliana	F26B6.11, At2g23460, XLG, XLG1	Extra-large guanine nucleotide-binding protein 1	0/1	0	0	0	Yes
Q63803	GNAS1_RAT	InParanoid	R. norvegicus	Gnas1, Gnas	Guanine nucleotide-binding protein G	0/3	6	0	2	Yes
M0R9J4	M0R9J4_RAT	Ensembl	R. norvegicus	Gnas	Neuroendocrine secretory protein 55	0/3	0	0	0	Yes

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:778	Hiol A et al.	2003	Mus musculus	Sf9	Cytosol	[3H]palmitic acid labeling

Sites (from validation or large-scale experiments)

Isoforms	Position	Probability	Studies	Techniques
Q6R0H7	1104	NA	SPalmE:903 Collins MO et al. (2017)*	ABE
Q6R0H7	913	NA	SPalmE:855 Shen LF et al. (2017)*	ARAC

Cysteine summary and predictions

Pos in alignment	6	86	267	299	351	655	656	665	681	716	722	778	901	913
Subcell. location	U	U	U	U	U	U	U	U	U	U	U	U	U	U
Disulfide	no	no	no	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	coil	helix	strand	coil	coil	coil	coil	coil	helix	coil	coil	coil	helix	helix
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁶ HC	Cys⁸⁶	Cys²⁶⁷	Cys²⁹⁹	Cys³⁵¹	Cys⁶⁵⁵ HC	Cys⁶⁵⁶ HC	Cys⁶⁶⁵	Cys⁶⁸¹	Cys⁷¹⁶	Cys⁷²² LC	Cys⁷⁷⁸	Cys⁹⁰¹	Cys⁹¹³
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys⁶ HC	Cys⁸⁶	Cys²⁶⁷	Cys²⁹⁹	Cys³⁵¹	Cys⁶⁵⁵ HC	Cys⁶⁵⁶ HC	Cys⁶⁶⁵	Cys⁶⁸¹	Cys⁷¹⁶	Cys⁷²² LC	Cys⁷⁷⁸
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys⁶ HC	Cys⁸⁶	Cys²⁶⁷	Cys²⁹⁹	Cys³⁵¹	Cys⁶⁵⁵ HC	Cys⁶⁵⁶ HC	Cys⁶⁶⁵	Cys⁶⁸¹	Cys⁷¹⁶	Cys⁷²² LC	Cys⁷⁷⁸
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys⁶ HC	Cys⁸⁶	Cys²⁶⁷	Cys²⁹⁹	Cys³⁵¹	Cys⁶⁵⁵ HC	Cys⁶⁵⁶ HC	Cys⁶⁶⁵	Cys⁶⁸¹	Cys⁷¹⁶	Cys⁷²² LC	Cys⁷⁷⁸	Cys⁸⁸⁷	Cys⁸⁹⁹

Pos in alignment	939	976	1075	1095	1097	1098	1104	1118
Subcell. location	U	U	U	U	U	U	U	U
Disulfide	no	no				no	no	no
PSIPRED v.4.02 prediction	strand	helix				strand	strand	helix
UniProt secondary structure	NA	NA				NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁹³⁹	Cys⁹⁷⁶				Cys¹⁰⁹⁸	Cys¹¹⁰⁴	Cys¹¹¹⁸
Pos in isoform 2 CSS-Palm 4.0 PalmPred			Cys⁸⁴⁶	Cys⁸⁶⁶ MC	Cys⁸⁶⁸		Cys⁸⁷⁵ MC
Pos in isoform 3 CSS-Palm 4.0 PalmPred
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys⁹²⁵	Cys⁹⁶²				Cys¹⁰⁸⁴	Cys¹⁰⁹⁰	Cys¹¹⁰⁴

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

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ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	Ortholog	in HC [ALEX, GNAS2, GNAS1, GNAS3]	HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	Ortholog	in HC [ALEX, GNAS2, GNAS1, GNAS3]	HC: 102 hits LC: 73 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	Ortholog	in HC [ALEX, GNAS2, GNAS1, GNAS3]	HC: 162 hits LC: 218 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 69 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	x		HC HC: 352 hits LC LC: 8 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 214 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	Ortholog	in HC [ALEX, GNAS2, GNAS1, GNAS3]	HC: 164 hits LC: 178 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 154 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	Ortholog	in HC [ALEX, GNAS2, GNAS1, GNAS3]	HC HC: 93 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	Ortholog	in HC [GPA1]	HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	x		List: 436 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 1348 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 274 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	x		List: 226 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 354 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 216 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 1353 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	Found	in List	List: 865 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	Found	in HC	HC: 1079 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Found	in Palm. up with LPS	Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Found	in List	List: 646 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Found	in LPS treatment	LPS treatment: 646 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Found	in HC	HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Found	in MC	HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Ortholog	in MC [ALEX, GNAS2, GNAS1, GNAS3]	HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	Ortholog	in HC [ALEX, GNAS2, GNAS1, GNAS3]	HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	Ortholog	in Trypsin [ALEX, GNAS2, GNAS1, GNAS3]	Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Found	in HC	HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Found	in HC	HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [GNAS2]	List: 52 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	Ortholog	in List [GNAS]	List: 157 hits
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 590 hits
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 2929 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 370 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x	in [ALEX, GNAS2, GNAS1, GNAS3]	Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 185 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [ALEX, GNAS2, GNAS1, GNAS3]	HC: 1869 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [ALEX, GNAS2, GNAS1, GNAS3]	HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [ALEX, GNAS2, GNAS1, GNAS3]	HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [ALEX, GNAS2, GNAS1, GNAS3]	HC: 600 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [GNAS2]	List: 80 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 687 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 399 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 464 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Ortholog	in List [ALEX, GNAS2, GNAS1, GNAS3]	List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 199 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1093 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1040 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Found	in HC	HC: 4107 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Found	in HC	HC: 3870 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	Found	in List	List: 1378 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Found	in List	List: 1597 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	0	0.0	NA
H. sapiens	22	38	33	86.8	ALEX_HUMAN , GNAS2_HUMAN , GNAS1_HUMAN , GNAS3_HUMAN
M. musculus	23	37	13	35.1	NA
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
S. pombe	1	1	0	0.0	NA
A. thaliana	1	1	1	100.0	GPA1_ARATH
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	2	100.0	GNAS2_BOVIN
D. melanogaster	1	2	1	50.0	GNAS_DROME
C. familiaris	1	1	0	0.0	NA

References

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