SwissPalm

Protein general information from UniProt

Protein names

Palmitoyltransferase ZDHHC3, EC 2.3.1.225; Acyltransferase ZDHHC3, EC 2.3.1.-; GABA-A receptor-associated membrane protein 1; Golgi-specific DHHC zinc finger protein; Zinc finger DHHC domain-containing protein 3, DHHC-3

Subcellular location

Golgi apparatus membrane

Isoforms & protein length

Isoform 1 (main): 299 aa

Known functions

Golgi-localized palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates (PubMed:15229235, PubMed:15603741, PubMed:16129400, PubMed:19001095, PubMed:23793055, PubMed:25253725, PubMed:26487721). Has no stringent fatty acid selectivity and in addition to palmitate can also transfer onto target proteins myristate from tetradecanoyl-CoA and stearate from octadecanoyl-CoA (Probable). Plays an important role in G protein-coupled receptor signaling pathways involving GNAQ and potentially other heterotrimeric G proteins by regulating their dynamic association with the plasma membrane (PubMed:19001095). Palmitoylates ITGA6 and ITGB4, thereby regulating the alpha-6/beta-4 integrin localization, expression and function in cell adhesion to laminin (By similarity). Plays a role in the TRAIL-activated apoptotic signaling pathway most probably through the palmitoylation and localization to the plasma membrane of TNFRSF10A (By similarity). In the brain, by palmitoylating the gamma subunit GABRG2 of GABA(A) receptors and regulating their postsynaptic accumulation, plays a role in synaptic GABAergic inhibitory function and GABAergic innervation (PubMed:15229235, PubMed:17151279, PubMed:27875292). Palmitoylates the neuronal protein GAP43 which is also involved in the formation of GABAergic synapses (PubMed:27875292). Palmitoylates NCDN thereby regulating its association with endosome membranes (PubMed:23687301). Probably palmitoylates PRCD and is involved in its proper localization within the photoreceptor (PubMed:27613864). Could mediate the palmitoylation of NCAM1 and regulate neurite outgrowth (PubMed:27247265). Could palmitoylate DNAJC5 and regulate its localization to Golgi membranes (PubMed:18596047). Also constitutively palmitoylates DLG4 (PubMed:15603741). May also palmitoylate SNAP25 (PubMed:25253725). Could palmitoylate the glutamate receptors GRIA1 and GRIA2 but this has not been confirmed in vivo (PubMed:16129400, PubMed:27875292). Could also palmitoylate the D(2) dopamine receptor DRD2 (By similarity). May also function as a calcium transporter. hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-hexadecanoyl-L-cysteinyl-[protein] L-cysteinyl-[protein] + tetradecanoyl-CoA = CoA + S-tetradecanoyl-L-cysteinyl-[protein] L-cysteinyl-[protein] + octadecanoyl-CoA = CoA + S-octadecanoyl-L-cysteinyl-[protein] Monomer (PubMed:23793055). Homooligomers (PubMed:17151279, PubMed:23793055). The monomeric form has a higher catalytic activity (PubMed:23793055). Forms heterooligomers with ZDHHC7 (PubMed:17151279). Interacts with TNFRSF10A (By similarity). Localizes to the Golgi cis cisterna. Widely expressed, with highest levels in brain. In the brain, expressed only in neurons (at protein level). The DHHC domain is required for palmitoyltransferase activity. Phosphorylation by FGFR1 and SRC probably regulates the palmitoyltransferase activity. Autopalmitoylated. Homozygous knockout mice are viable, fertile and do not display overt phenotype (PubMed:27875292). Male show a modest reduction in body weight (PubMed:27875292). Zdhhc3 and Zdhhc7 double knockout mice show a perinatally lethal phenotype (PubMed:27875292). Belongs to the DHHC palmitoyltransferase family. Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability (PubMed:15229235). Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi (PubMed:16129400). May also palmitoylate DLG4, DNAJC5 and SNAP25 (PubMed:15603741, PubMed:25253725). hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-hexadecanoyl-L-cysteinyl-[protein] Widely expressed, with highest levels in brain. In the brain, expressed only in neurons (at protein level). The DHHC domain is required for palmitoyltransferase activity. Autopalmitoylated. Belongs to the DHHC palmitoyltransferase family. Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability (PubMed:15229235). Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi (PubMed:16129400). May also palmitoylate DLG4, DNAJC5 and SNAP25 (PubMed:15603741, PubMed:25253725). Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA. Widely expressed, with highest levels in brain. In the brain, expressed only in neurons (at protein level). The DHHC domain is required for palmitoyltransferase activity. Autopalmitoylated. Belongs to the DHHC palmitoyltransferase family.

Cross-references

Source	Identifiers
UniProt gene names	Zdhhc3, Godz, Gramp1
UniProt secondary AC	Q6EMK3
Refseq protein ID	NP_081193.2
IPI ID	IPI00172092, IPI00112074

Source	Identifiers
Ensembl protein	ENSMUSP00000117392
Ensembl gene	ENSMUSG00000025786
MGI ID	MGI:1926134
BrainPalmSeq	zdhhcs-2/search-by-gene-zdhhc3

Enzymes

PAT/APT	Enzyme	Experiments
PAT	ZDHC3_MOUSE	SPalmE:898 Spinelli M et al. (2017) SPalmE:1003 Hines RM et al. (2010) SPalmE:1002 Hines RM et al. (2010)
PAT	ZDH17_MOUSE	SPalmE:1003 Hines RM et al. (2010) SPalmE:1002 Hines RM et al. (2010)

Protein acyltransferase activity (PAT)

Substrate UniProt ID	Substrate description	Substrate isoform AC (site position)	Experiments
GRIA1_MOUSE	Glutamate receptor 1, GluR-1	P23818 (603)	SPalmE:157 Hayashi T et al. (2005) SPalmE:897 Spinelli M et al. (2017)
GRIA2_MOUSE	Glutamate receptor 2, GluR-2	P23819 (610)	SPalmE:157 Hayashi T et al. (2005) SPalmE:897 Spinelli M et al. (2017)
DLG4_MOUSE	Disks large homolog 4	Q62108	SPalmE:144 Fukata M et al. (2004) SPalmE:685 Suzuki M et al. (2015)
DNJC5_BOVIN	DnaJ homolog subfamily C member 5	Q29455	SPalmE:142 Greaves J et al. (2008)
Q9EQ27_MOUSE	Ca2+ voltage sensitive potassium channel STREX variant	Q9EQ27 (12,13)	SPalmE:182 Tian L et al. (2010)
NCAM1_RAT	Neural cell adhesion molecule 1, N-CAM-1, NCAM-1	P13596-1 P13596	SPalmE:167 Ponimaskin E et al. (2008) SPalmE:168 Ponimaskin E et al. (2008) SPalmE:753 Lievens PM et al. (2016)
BACE1_MOUSE	Beta-secretase 1, EC 3.4.23.46	P56818	SPalmE:173 Vetrivel KS et al. (2009)
KCC1G_MOUSE	Calcium/calmodulin-dependent protein kinase type 1G, EC 2.7.11.17	Q91VB2	SPalmE:170 Takemoto-Kimura S et al. (2007)
NMDE1_RAT	Glutamate receptor ionotropic, NMDA 2A, GluN2A	Q00959	SPalmE:163 Hayashi T et al. (2009)
NMDE2_RAT	Glutamate receptor ionotropic, NMDA 2B, GluN2B	Q00960	SPalmE:163 Hayashi T et al. (2009)
GBRG2_MOUSE	Gamma-aminobutyric acid receptor subunit gamma-2	P22723	SPalmE:178 Fang C et al. (2006) SPalmE:218 Keller CA et al. (2004)
HD_MOUSE	Huntingtin	P42859	SPalmE:177 Saleem AN et al. (2010)
OPRM_HUMAN	Mu-type opioid receptor, M-OR-1, MOR-1	P35372	SPalmE:324 Ebersole B et al. (2014)
CCG2_RAT	Voltage-dependent calcium channel gamma-2 subunit	Q71RJ2	SPalmE:249 Oku S et al. (2013)
CNIH2_RAT	Protein cornichon homolog 2, CNIH-2	Q5BJU5	SPalmE:249 Oku S et al. (2013)
TRPM8_MOUSE	Transient receptor potential cation channel subfamily M member 8	Q8R4D5	SPalmE:249 Oku S et al. (2013)
TRPC1_HUMAN	Short transient receptor potential channel 1, TrpC1	P48995	SPalmE:249 Oku S et al. (2013)
OX2R_MOUSE	Orexin receptor type 2, Ox-2-R, Ox2-R, Ox2R	P58308	SPalmE:249 Oku S et al. (2013)
ZYX_HUMAN	Zyxin	Q15942	SPalmE:249 Oku S et al. (2013)
CCG8_RAT	Voltage-dependent calcium channel gamma-8 subunit	Q8VHW5	SPalmE:249 Oku S et al. (2013)
KCC2A_RAT	Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, EC 2.7.11.17	P11275	SPalmE:249 Oku S et al. (2013)
NCDN_RAT	Neurochondrin	O35095	SPalmE:249 Oku S et al. (2013)
BAX_HUMAN	Apoptosis regulator BAX	Q07812 (126)	SPalmE:302 Fröhlich M et al. (2014)
SNP25_RAT	Synaptosomal-associated protein 25, SNAP-25	P60881	SPalmE:418 Lemonidis K et al. (2014)
DNJC5_RAT	DnaJ homolog subfamily C member 5	P60905	SPalmE:418 Lemonidis K et al. (2014)
TLR2_HUMAN	Toll-like receptor 2	O60603 (609)	SPalmE:581 Chesarino NM et al. (2014)
CD86_MOUSE	T-lymphocyte activation antigen CD86	P42082 (264)	SPalmE:581 Chesarino NM et al. (2014)
PPT1_HUMAN	Palmitoyl-protein thioesterase 1, PPT-1, EC 3.1.2.22	P50897 (6)	SPalmE:634 Segal-Salto M et al. (2016) SPalmE:742 Segal-Salto M et al. (2016)
GNAQ_HUMAN	Guanine nucleotide-binding protein G	P50148 (9,10)	SPalmE:110 Tsutsumi R et al. (2009) SPalmE:664 Tsutsumi R et al. (2009)
GNAS2_HUMAN	Guanine nucleotide-binding protein G	P63092	SPalmE:112 Tsutsumi R et al. (2009)
GNAI2_HUMAN	Guanine nucleotide-binding protein G	P04899	SPalmE:112 Tsutsumi R et al. (2009)
ELMD2_HUMAN	ELMO domain-containing protein 2	Q8IZ81-1 (94,98)	SPalmE:685 Suzuki M et al. (2015)
PPT1_MOUSE	Palmitoyl-protein thioesterase 1, PPT-1, EC 3.1.2.22	O88531	SPalmE:743 Segal-Salto M et al. (2016)
STING_MOUSE	Stimulator of interferon genes protein, mSTING	Q3TBT3 (88,91)	SPalmE:762 Mukai K et al. (2016)
NEUM_MOUSE	Neuromodulin	P06837 (3,4)	SPalmE:856 Kilpatrick CL et al. (2016)
F8RGC5_HHV1	Envelope protein UL20	F8RGC5	SPalmE:887 Wang S et al. (2017) SPalmE:891 Wang S et al. (2017)
CAHM1_MOUSE	Calcium homeostasis modulator protein 1	D3Z291 (100,207)	SPalmE:889 Taruno A et al. (2017)
ZDHC3_MOUSE	Palmitoyltransferase ZDHHC3, EC 2.3.1.225	Q8R173	SPalmE:898 Spinelli M et al. (2017) SPalmE:1003 Hines RM et al. (2010) SPalmE:1002 Hines RM et al. (2010)
MSHR_HUMAN	Melanocyte-stimulating hormone receptor, MSH-R	Q01726 (315)	SPalmE:991 Chen S et al. (2017) SPalmE:990 Chen S et al. (2017)
SYDE1_MOUSE	Rho GTPase-activating protein SYDE1	Q9DBZ9	SPalmE:249 Oku S et al. (2013)
STX19_HUMAN	Syntaxin-19	Q8N4C7 (284,285,287,288,290,291)	SPalmE:1180 Ampah KK et al. (2018)
SC6A3_RAT	Sodium-dependent dopamine transporter, DA transporter, DAT	P23977	SPalmE:1239 Bolland DE et al. (2019) [] SPalmE:1240 Bolland DE et al. (2019) []

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

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UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Predicted
Q9NYG2	ZDHC3_HUMAN	Ensembl, InParanoid	H. sapiens	ZDHHC3, ZNF373, HSD49	Palmitoyltransferase ZDHHC3, EC 2.3.1.225	7/22	0	4	Yes
F6UMM9	F6UMM9_CANLF	Ensembl	C. familiaris	ZDHHC3	Palmitoyltransferase	0/1	0	0	Yes
E2RND6	E2RND6_CANLF	Ensembl	C. familiaris	ZDHHC3	Palmitoyltransferase	0/1	0	0	Yes
F1MVC6	F1MVC6_BOVIN	Ensembl, MetaPhors	B. taurus		Palmitoyltransferase	0/2	0	0	Yes
E2RND4	E2RND4_CANLF	OMA, Ensembl, InParanoid	C. familiaris	ZDHHC3	Palmitoyltransferase	0/1	0	0	Yes
Q2TGK3	ZDHC3_RAT	OMA, Ensembl, InParanoid, MetaPhors	R. norvegicus	Zdhhc3, LOC683192, rCG_25574	Palmitoyltransferase ZDHHC3, EC 2.3.1.225	0/3	1	0	Yes
Q08DX8	Q08DX8_BOVIN	OMA, InParanoid, MetaPhors	B. taurus	ZDHHC3	Palmitoyltransferase	0/2	0	0	Yes
Q7K3T4	Q7K3T4_DROME	OMA, Ensembl, InParanoid, MetaPhors	D. melanogaster	Dmel\CG8314, anon-WO0118547.93, CG8314, Dmel_CG8314	Palmitoyltransferase	1/1	0	2	Yes
F8W6M3	F8W6M3_HUMAN	OMA, Ensembl, MetaPhors	H. sapiens	ZDHHC3	Palmitoyltransferase	0/22	0	0	Yes
H0Y6A5	H0Y6A5_HUMAN	Ensembl	H. sapiens	ZDHHC3	Palmitoyltransferase	0/22	0	0	Yes
C9JIA9	C9JIA9_HUMAN	Ensembl	H. sapiens	ZDHHC3	Palmitoyltransferase	0/22	0	0	Yes
H7C365	H7C365_HUMAN	Ensembl	H. sapiens	ZDHHC3	Palmitoyltransferase ZDHHC3	0/22	0	0	No
O96238	O96238_PLAF7	InParanoid	P. falciparum	PF3D7_0215900	Palmitoyltransferase	0/1	0	0	Yes

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:110	Tsutsumi R et al.	2009	Homo sapiens	HEK293T	Total cell lysate	NH2OH cleavage of thioester bond, PAT overexpression, PAT point mutation, Point mutation, [3H]palmitic acid labeling
SPalmE:112	Tsutsumi R et al.	2009	Homo sapiens	HEK293T	Total cell lysate	PAT overexpression, [3H]palmitic acid labeling
SPalmE:142	Greaves J et al.	2008	Homo sapiens	HEK293	Total cell lysate	PAT overexpression, PAT point mutation, [3H]palmitic acid labeling
SPalmE:144	Fukata M et al.	2004	Chlorocebus aethiops	COS7	Total cell lysate	PAT overexpression, [3H]palmitic acid labeling
SPalmE:157	Hayashi T et al.	2005	Homo sapiens	HEK293T	Total cell lysate	PAT overexpression, PAT point mutation
SPalmE:163	Hayashi T et al.	2009	Homo sapiens	HEK293T	Total cell lysate	PAT overexpression, PAT point mutation, [3H]palmitic acid labeling
SPalmE:167	Ponimaskin E et al.	2008	Homo sapiens	HEK293	Total cell lysate	PAT overexpression, Substrate truncation, [3H]palmitic acid labeling
SPalmE:168	Ponimaskin E et al.	2008	Mus musculus	Neuro-2a	Total cell lysate	PAT overexpression, [3H]palmitic acid labeling
SPalmE:177	Saleem AN et al.	2010	Homo sapiens	HEK293T	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT overexpression, PAT point mutation
SPalmE:178	Fang C et al.	2006	Homo sapiens	HEK293T	Total cell lysate	2-HYB, 2BP inhibition, PAT localization by IF, PAT overexpression, Point mutation, Substrate localization by fluorescence tag, [3H]palmitic acid labeling
SPalmE:182	Tian L et al.	2010	Homo sapiens	HEK293	Total cell lysate	2BP inhibition, Co-IP of PAT and substrate, Depletion of PAT by siRNA, PAT overexpression, Point mutation, Substrate truncation, [3H]palmitic acid labeling
SPalmE:218	Keller CA et al.	2004	Mus musculus	HEK293T	Total cell lysate	NH2OH cleavage of thioester bond, [3H]palmitic acid labeling
SPalmE:249	Oku S et al.	2013	Rattus norvegicus	Hippocampal neuron	Total cell lysate	ABE, NH2OH cleavage of thioester bond, [3H]palmitic acid labeling
SPalmE:302	Fröhlich M et al.	2014	Homo sapiens	HEK293	Non-membrane lysate	2BP inhibition, ABE, NH2OH cleavage of thioester bond, PAT overexpression, Point mutation
SPalmE:324	Ebersole B et al.	2014	Homo sapiens	HEK293	Total cell lysate	15-HDYA labeling, Click chemistry
SPalmE:418	Lemonidis K et al.	2014	Homo sapiens	HEK293T	Total cell lysate	Co-IP of PAT and substrate, PAT localization by IF, PAT overexpression, [3H]palmitic acid labeling
SPalmE:581	Chesarino NM et al.	2014	Homo sapiens	HEK293T	Total cell lysate	2BP inhibition, Click chemistry, PAT overexpression, Point mutation
SPalmE:634	Segal-Salto M et al.	2016	Homo sapiens	HEK293	Total cell lysate	Click chemistry, NH2OH cleavage of thioester bond, PAT dominant-negative overexpression, PAT overexpression, Point mutation
SPalmE:664	Tsutsumi R et al.	2009	Homo sapiens	NA	NA	PAT overexpression, Point mutation, [3H]palmitic acid labeling
SPalmE:685	Suzuki M et al.	2015	Homo sapiens	HEK293T	Total cell lysate	PAT overexpression, Point mutation, [3H]palmitic acid labeling
SPalmE:742	Segal-Salto M et al.	2016	Homo sapiens	HEK293	Total cell lysate	Click chemistry, NH2OH cleavage of thioester bond, PAT overexpression, Point mutation
SPalmE:743	Segal-Salto M et al.	2016	Homo sapiens	HEK293	Total cell lysate	Click chemistry, PAT overexpression
SPalmE:753	Lievens PM et al.	2016	Mus musculus	Neuro-2a	Total cell lysate	PAT overexpression, [3H]palmitic acid labeling
SPalmE:755	Lievens PM et al.	2016	Mus musculus	Neuro-2a	Total cell lysate	Click-IT Palmitic Acid labeling
SPalmE:762	Mukai K et al.	2016	Mus musculus	COS1	Total cell lysate	2BP inhibition, NH2OH cleavage of thioester bond, PAT localization by fluorescent tag, PAT overexpression, Point mutation, Substrate localization by fluorescence tag, [3H]palmitic acid labeling
SPalmE:856	Kilpatrick CL et al.	2016	Mus musculus	HEK293T	Total cell lysate	ABE, NH2OH cleavage of thioester bond, Point mutation
SPalmE:887	Wang S et al.	2017	Human herpesvirus 1	HeLa	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT point mutation
SPalmE:889	Taruno A et al.	2017	Mus musculus	Neuro-2a	Total cell lysate	2BP inhibition, ABE, Click chemistry, Depletion of PAT by siRNA, NH2OH cleavage of thioester bond, Point mutation
SPalmE:891	Wang S et al.	2018	Human herpesvirus 1	MEF	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO
SPalmE:897	Spinelli M et al.	2017	Mus musculus	Hippocampal neuron	Total cell lysate	ABE
SPalmE:898	Spinelli M et al.	2017	Mus musculus	Hippocampal neuron	Total cell lysate	2BP inhibition, ABE
SPalmE:991	Chen S et al.	2017	Homo sapiens	Human primary melanocytes	Total cell lysate	2BP inhibition, ABE, Co-IP of PAT and substrate, Depletion of PAT by shRNA, MS, NH2OH cleavage of thioester bond, PAT overexpression, Palmostatin B inhibition, Point mutation
SPalmE:1002	Hines RM et al.	2010	Xenopus laevis	xenopus ovocytes	Total cell lysate	2BP inhibition, ABE, NH2OH cleavage of thioester bond, PAT overexpression, PAT point mutation, Point mutation, Substrate localization by fluorescence tag
SPalmE:1003	Hines RM et al.	2010	Homo sapiens	COS7	Total cell lysate	2BP inhibition, ABE, NH2OH cleavage of thioester bond, PAT overexpression, PAT point mutation, Point mutation, Substrate localization by fluorescence tag
SPalmE:1239	Bolland DE et al.	2019	Sus scrofa	LLC-PK1	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT overexpression, [3H]palmitic acid labeling
SPalmE:1240	Bolland DE et al.	2019	Rattus norvegicus	Neuro-2a	Total cell lysate	2BP inhibition, ABE, NH2OH cleavage of thioester bond, PAT overexpression, [3H]palmitic acid labeling
SPalmE:170	Takemoto-Kimura S et al.	2007	Chlorocebus aethiops	COS7	Total cell lysate	PAT overexpression, [3H]palmitic acid labeling
SPalmE:173	Vetrivel KS et al.	2009	Chlorocebus aethiops	COS7	Total cell lysate	PAT overexpression, [3H]palmitic acid labeling
SPalmE:990	Chen S et al.	2017	Homo sapiens	B16 melanoma	Total cell lysate	2BP inhibition, ABE, MS, NH2OH cleavage of thioester bond, Palmostatin B inhibition, Point mutation
SPalmE:1180	Ampah KK et al.	2018	Homo sapiens	HEK293T	Total cell lysate	, , Click chemistry, LC, MS, NH2OH cleavage of thioester bond, PAT overexpression

Cysteine summary and predictions

Pos in alignment	24	43	47	95	129	132	133	143	146	149	157	163	197	205
Subcell. location	C	C	C	C	C	C	C	C	C	C	C	C	E	E
Disulfide	no	no	no	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	coil	helix	helix	helix	coil	coil	coil	coil	coil	strand	coil	helix	helix	coil
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys²⁴	Cys⁴³ HC	Cys⁴⁷ LC	Cys⁹⁵	Cys¹²⁹	Cys¹³² HC	Cys¹³³ HC	Cys¹⁴³	Cys¹⁴⁶	Cys¹⁴⁹	Cys¹⁵⁷	Cys¹⁶³	Cys¹⁹⁷	Cys²⁰⁵ HC

Pos in alignment	221	243
Subcell. location	T	C
Disulfide	no	no
PSIPRED v.4.02 prediction	helix	helix
UniProt secondary structure	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys²²¹	Cys²⁴³

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

Tip: Keep CTRL key pressed down while clicking on column headers to do multiple sorting. This applies to any sortable table on this site.

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	x		HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	x		HC: 162 hits LC: 218 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	x		HC: 102 hits LC: 73 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	x		HC HC: 352 hits LC LC: 8 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	x		HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	Found	in List	List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	x		List: 436 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	x		Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	Ortholog	in List [ZDHC3]	List: 354 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Ortholog	in List [ZDHC3]	List: 1348 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	Ortholog	in List [ZDHC3]	List: 354 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	x		List: 226 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	x		List: 274 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	x		List: 1353 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	x		HC: 1079 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		List: 865 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	Found	in LC	HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	Ortholog	in List [ZDHC3]	List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	Ortholog	in List [ZDHC3]	List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Found	in MC	HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Found	in LPS treatment	LPS treatment: 646 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Found	in List	List: 646 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Found	in LC	HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	Ortholog	in List [Q7K3T4]	List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	Ortholog	in List [ZDHC3]	List: 2929 hits
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [ZDHC3]	List: 590 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 370 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [ZDHC3]	HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [ZDHC3]	HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 600 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x	in [ZDHC3]	Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	Ortholog	in List [ZDHC3]	List: 185 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Ortholog	in HC [ZDHC3]	HC: 1869 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1093 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 687 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Ortholog	in List [ZDHC3]	List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Ortholog	in List [ZDHC3]	List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 199 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 399 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1040 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 464 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 4107 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1597 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1378 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 3870 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	0	0.0	NA
H. sapiens	22	38	14	36.8	ZDHC3_HUMAN
M. musculus	23	37	6	16.2	NA
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
A. thaliana	1	1	0	0.0	NA
S. pombe	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	1	50.0	Q7K3T4_DROME
C. familiaris	1	1	0	0.0	NA

References

Authors	Year	Reference title	PMID
Lemonidis K et al.	2014	The Golgi S-acylation machinery comprises zDHHC enzymes with major differences in substrate affinity and S-acylation activity.	25253725
Suzuki M et al.	2015	ELMOD2 is anchored to lipid droplets by palmitoylation and regulates adipocyte triglyceride lipase recruitment.	25904333
Mariscal J et al.	2020	Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles.	32944167
Chen S et al.	2017	Palmitoylation-dependent activation of MC1R prevents melanomagenesis.	28869973
Marin EP et al.	2012	Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases.	22496122
Thinon E et al.	2018	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.	29575903
Hayashi T et al.	2009	Dual palmitoylation of NR2 subunits regulates NMDA receptor trafficking.	19874789
Peng T et al.	2015	Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.	25575299
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Ziemlińska E et al.	2021	Palm Oil-Rich Diet Affects Murine Liver Proteome and <i>S</i>-Palmitoylome.	34884899
Hayashi T et al.	2005	Differential regulation of AMPA receptor subunit trafficking by palmitoylation of two distinct sites.	16129400
Ivaldi C et al.	2012	Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.	22615937
Colquhoun DR et al.	2015	Bioorthogonal mimetics of palmitoyl-CoA and myristoyl-CoA and their subsequent isolation by click chemistry and characterization by mass spectrometry reveal novel acylated host-proteins modified by HIV-1 infection.	25914232
Gould NS et al.	2015	Site-Specific Proteomic Mapping Identifies Selectively Modified Regulatory Cysteine Residues in Functionally Distinct Protein Networks.	26165157
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
Ren W et al.	2013	Proteomic analysis of protein palmitoylation in adipocytes.	23599907
Lievens PM et al.	2016	ZDHHC3 Tyrosine Phosphorylation Regulates Neural Cell Adhesion Molecule Palmitoylation.	27247265
Ripamonti M et al.	2021	Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions.	33782527
Morrison E et al.	2020	Dynamic palmitoylation events following T-cell receptor signaling.	32651440
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Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Emmer BT et al.	2011	Global analysis of protein palmitoylation in African trypanosomes.	21193548
Zhou B et al.	2019	Low-background Acyl-biotinyl Exchange Largely Eliminates the Co-isolation of Non-S-acylated Proteins and Enables Deep S-acylproteomic Analysis.	31251020
Martin BR et al.	2009	Large-scale profiling of protein palmitoylation in mammalian cells.	19137006
Hines RM et al.	2010	Golgi-specific DHHC zinc finger protein GODZ mediates membrane Ca2+ transport.	19955568
Martin BR et al.	2011	Global profiling of dynamic protein palmitoylation.	22056678
Tsutsumi R et al.	2009	Identification of G protein alpha subunit-palmitoylating enzyme.	19001095
Mukai K et al.	2016	Activation of STING requires palmitoylation at the Golgi.	27324217
Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
Wang J et al.	2019	DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane.	30605677
Pinner AL et al.	2016	Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia.	26876311
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Morrison E et al.	2015	Quantitative analysis of the human T cell palmitome.	26111759
Wang S et al.	2018	The Absence of DHHC3 Affects Primary and Latent Herpes Simplex Virus 1 Infection.	29187538
Wan J et al.	2013	Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease.	24211138
Merrick BA et al.	2011	Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3.	21785166
Thinon E et al.	2016	Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.	27350074
Greaves J et al.	2008	Palmitoylation and membrane interactions of the neuroprotective chaperone cysteine-string protein.	18596047
Miles MR et al.	2021	Global identification of S-palmitoylated proteins and detection of palmitoylating (DHHC) enzymes in heart.	33636221
Zhang X et al.	2018	Ultradeep Palmitoylomics Enabled by Dithiodipyridine-Functionalized Magnetic Nanoparticles.	29660268
Caballero MC et al.	2016	Identification of new palmitoylated proteins in Toxoplasma gondii.	26825284
Hernandez JL et al.	2016	Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.	27030425
Kilpatrick CL et al.	2016	Dissociation of Golgi-associated DHHC-type Zinc Finger Protein (GODZ)- and Sertoli Cell Gene with a Zinc Finger Domain-β (SERZ-β)-mediated Palmitoylation by Loss of Function Analyses in Knock-out Mice.	27875292
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
Taruno A et al.	2017	Post-translational palmitoylation controls the voltage gating and lipid raft association of the CALHM1 channel.	28734079
Vetrivel KS et al.	2009	Alzheimer disease Abeta production in the absence of S-palmitoylation-dependent targeting of BACE1 to lipid rafts.	19074428
Wang S et al.	2017	Binding of Herpes Simplex Virus 1 UL20 to GODZ (DHHC3) Affects Its Palmitoylation and Is Essential for Infectivity and Proper Targeting and Localization of UL20 and Glycoprotein K.	28724772
Ponimaskin E et al.	2008	Fibroblast growth factor-regulated palmitoylation of the neural cell adhesion molecule determines neuronal morphogenesis.	18768683
Sobocińska J et al.	2018	Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.	29217618
Oku S et al.	2013	In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes.	23687301
Zaręba-Kozioł M et al.	2019	Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins.	31311849
Hemsley PA et al.	2013	A proteomic approach identifies many novel palmitoylated proteins in Arabidopsis.	23252521
Chesarino NM et al.	2014	Chemoproteomics reveals Toll-like receptor fatty acylation.	25371237
Fang C et al.	2006	GODZ-mediated palmitoylation of GABA(A) receptors is required for normal assembly and function of GABAergic inhibitory synapses.	17151279
Zhang MM et al.	2013	Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.	23843742
Wegleiter T et al.	2019	Palmitoylation of BMPR1a regulates neural stem cell fate.	31772009
Ampah KK et al.	2018	S-acylation regulates the trafficking and stability of the unconventional Q-SNARE STX19.	30254024
Pradhan AJ et al.	2021	Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.	33848465
Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
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Strassburger K et al.	2019	Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.	30735487
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Zaręba-Kozioł M et al.	2021	S-Palmitoylation of Synaptic Proteins as a Novel Mechanism Underlying Sex-Dependent Differences in Neuronal Plasticity.	34200797
Uzbekova S et al.	2021	Protein Palmitoylation in Bovine Ovarian Follicle.	34769186
Dumoulin A et al.	2018	S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.	30319353
Gorenberg EL et al.	2022	Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.	35358180
Tian L et al.	2010	Multiple palmitoyltransferases are required for palmitoylation-dependent regulation of large conductance calcium- and voltage-activated potassium channels.	20507996
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Wei X et al.	2014	Insulin-regulated protein palmitoylation impacts endothelial cell function.	24357059
Forrester MT et al.	2011	Site-specific analysis of protein S-acylation by resin-assisted capture.	21044946
Fukata M et al.	2004	Identification of PSD-95 palmitoylating enzymes.	15603741
Spinelli M et al.	2017	Brain insulin resistance impairs hippocampal synaptic plasticity and memory by increasing GluA1 palmitoylation through FoxO3a.	29222408
Yang W et al.	2010	Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes.	19801377
Jones ML et al.	2012	Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis.	22901544
Ebersole B et al.	2014	Bioorthogonal click chemistry to assay mu-opioid receptor palmitoylation using 15-hexadecynoic acid and immunoprecipitation.	24463015
Keller CA et al.	2004	The gamma2 subunit of GABA(A) receptors is a substrate for palmitoylation by GODZ.	15229235
Kang R et al.	2008	Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.	19092927
Bolland DE et al.	2019	Palmitoylation by Multiple DHHC Enzymes Enhances Dopamine Transporter Function and Stability.	30965003
Saleem AN et al.	2010	Mice with alopecia, osteoporosis, and systemic amyloidosis due to mutation in Zdhhc13, a gene coding for palmitoyl acyltransferase.	20548961
Gao J et al.	2022	Proteome-wide identification of palmitoylated proteins in mouse testis.	35477839
Takemoto-Kimura S et al.	2007	Regulation of dendritogenesis via a lipid-raft-associated Ca2+/calmodulin-dependent protein kinase CLICK-III/CaMKIgamma.	17553424
Fröhlich M et al.	2014	S-palmitoylation represents a novel mechanism regulating the mitochondrial targeting of BAX and initiation of apoptosis.	24525733

Q8R173 ZDHC3_MOUSE