SwissPalm

Q96PN6 ADCYA_HUMAN

General info Cross-references Sequences Orthologs Ortholog sequences Predictions Palmitoyl-proteomes References

Palmitoyl-proteomes

1/22 article for human
0 ortholog

Palmitoyl-proteome experiments

Validation experiments

0 experiment
0 ortholog

Curated sites & Enzymes

0 site from validation experiments^*
0 site from palmitoyl-proteomes^*
No PAT known
No APT known
^*In main isoform only

Predictions

Not predicted

Predictions

Protein general information from UniProt

Protein names

Adenylate cyclase type 10, EC 4.6.1.1; AH-related protein; Adenylate cyclase homolog; Germ cell soluble adenylyl cyclase, hsAC, sAC; Testicular soluble adenylyl cyclase

Subcellular location

Cilium (Potential); Perinuclear region (Probable); Cytoskeleton (Probable); Mitochondrion (Probable); cilium; Cell projection; Nucleus (Probable); perinuclear region; cytoskeleton; Cytoplasm (Probable); Cell membrane (By similarity)

Isoforms & protein length

Isoform 1 (main): 1610 aa; Isoform 2 : 1518 aa; Isoform 3 : 372 aa; Isoform 4 : 1457 aa

Known functions

Catalyzes the formation of the signaling molecule cAMP (PubMed:12609998, PubMed:15659711, PubMed:24616449, PubMed:25040695, PubMed:24567411). May function as sensor that mediates responses to changes in cellular bicarbonate and CO(2) levels (PubMed:15659711, PubMed:17591988). Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization (By similarity). Involved in ciliary beat regulation (PubMed:17591988). ATP = 3',5'-cyclic AMP + diphosphate. Binds 2 magnesium ions per subunit (PubMed:25040695). Is also active with manganese (in vitro) (PubMed:12609998, PubMed:15659711, PubMed:24616449). Activated by manganese or magnesium ions (PubMed:12609998, PubMed:24616449). In the presence of magnesium ions, the enzyme is activated by bicarbonate (PubMed:12609998, PubMed:15659711, PubMed:24567411). In the presence of manganese ions, the enzyme is inhibited by bicarbonate (PubMed:15659711). In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium (PubMed:15659711). Calcium mildly increases the enzyme activity, also in the presence of magnesium ions (PubMed:15659711, PubMed:25040695). Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane-associated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin. Catalyzes the formation of the signaling molecule cAMP (PubMed:12609998, PubMed:15659711, PubMed:24616449, PubMed:25040695, PubMed:24567411). May function as sensor that mediates responses to changes in cellular bicarbonate and CO(2) levels (PubMed:15659711, PubMed:17591988). Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization (By similarity). Involved in ciliary beat regulation (PubMed:17591988). ATP = 3',5'-cyclic AMP + diphosphate Binds 2 magnesium ions per subunit (PubMed:25040695). Is also active with manganese (in vitro) (PubMed:12609998, PubMed:15659711, PubMed:24616449). Activated by manganese or magnesium ions (PubMed:12609998, PubMed:24616449). In the presence of magnesium ions, the enzyme is activated by bicarbonate (PubMed:12609998, PubMed:15659711, PubMed:24567411). In the presence of manganese ions, the enzyme is inhibited by bicarbonate (PubMed:15659711). In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium (PubMed:15659711). Calcium mildly increases the enzyme activity, also in the presence of magnesium ions (PubMed:15659711, PubMed:25040695). Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane-associated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin. Catalyzes the formation of the signaling molecule cAMP (PubMed:12609998, PubMed:15659711, PubMed:24616449, PubMed:25040695, PubMed:24567411). May function as sensor that mediates responses to changes in cellular bicarbonate and CO(2) levels (PubMed:15659711, PubMed:17591988). Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization (By similarity). Involved in ciliary beat regulation (PubMed:17591988). ATP = 3',5'-cyclic AMP + diphosphate Binds 2 magnesium ions per subunit (PubMed:25040695). Is also active with manganese (in vitro) (PubMed:12609998, PubMed:15659711, PubMed:24616449). Activated by manganese or magnesium ions (PubMed:12609998, PubMed:24616449). In the presence of magnesium ions, the enzyme is activated by bicarbonate (PubMed:12609998, PubMed:15659711, PubMed:24567411). In the presence of manganese ions, the enzyme is inhibited by bicarbonate (PubMed:15659711). In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium (PubMed:15659711). Calcium mildly increases the enzyme activity, also in the presence of magnesium ions (PubMed:15659711, PubMed:25040695). Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane-associated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin.

Cross-references

Source	Identifiers
UniProt gene names	SAC, ADCY10
UniProt secondary AC	Q9NNX0, Q8WXV4, Q5R330, Q5R329, O95558, F5GWS5, B4DZF0
Refseq protein ID	XP_005245386.1, NP_060887.2, NP_001161221.1, XP_011508067.1, XP_011508062.1, NP_001284701.1
IPI ID	IPI00299111, IPI00843800, IPI00742726, IPI00641214, IPI00514661

Source	Identifiers
Ensembl protein	ENSP00000441992, ENSP00000356825, ENSP00000356822
Ensembl gene	ENSG00000143199

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

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UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Predicted
Q8C0T9	ADCYA_MOUSE	MetaPhors, Ensembl, OrthoDB, OMA, InParanoid	M. musculus	Sacy, Sac, Adcy10	Adenylate cyclase type 10, EC 4.6.1.1	0/23	Yes
Q9Z286	ADCYA_RAT	MetaPhors, Ensembl, OrthoDB, OMA, InParanoid	R. norvegicus	Sac, Adcy10	Adenylate cyclase type 10, EC 4.6.1.1	0/3	Yes
E2RDZ3	E2RDZ3_CANLF	OMA, InParanoid	C. familiaris	ADCY10	Adenylate cyclase 10	0/1	No
E1B9W6	E1B9W6_BOVIN	OMA, MetaPhors, InParanoid	B. taurus	ADCY10	Adenylate cyclase 10	0/2	No
Q9VK52	Q9VK52_DROME	MetaPhors	D. melanogaster	Dmel_CG17174, CG17174, Dmel\CG17174, DACXB, Ac34A, AC34A, AC, ACXB	Adenylyl cyclase X B	0/1	Yes

Ortholog sequence alignment (CLUSTALW)

Cysteine summary and predictions

Pos in alignment	122	193	197	233	253	342	346	365	373	393	427	475	478	570
Subcell. location	C	C	C	C	C	C	C	C	C	C	C	C	C	C
Disulfide	no	no	no	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	helix	helix	helix	helix	helix	coil	strand	helix	helix	strand	strand	helix	helix	coil
UniProt secondary structure	helix	helix	NA	helix	helix	strand	strand	helix	helix	strand	strand	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys¹²²	Cys¹⁹³	Cys¹⁹⁷	Cys²³³	Cys²⁵³	Cys³⁴² MC	Cys³⁴⁶	Cys³⁶⁵	Cys³⁷³	Cys³⁹³	Cys⁴²⁷	Cys⁴⁷⁵	Cys⁴⁷⁸ MC	Cys⁵⁷⁰ MC
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys³⁰	Cys¹⁰¹	Cys¹⁰⁵	Cys¹⁴¹	Cys¹⁶¹	Cys²⁵⁰ MC	Cys²⁵⁴	Cys²⁷³	Cys²⁸¹	Cys³⁰¹	Cys³³⁵	Cys³⁸³	Cys³⁸⁶ MC	Cys⁴⁷⁸ MC
Pos in isoform 3 CSS-Palm 4.0 PalmPred
Pos in isoform 4 CSS-Palm 4.0 PalmPred		Cys⁴⁰	Cys⁴⁴	Cys⁸⁰	Cys¹⁰⁰	Cys¹⁸⁹ MC	Cys¹⁹³	Cys²¹²	Cys²²⁰	Cys²⁴⁰	Cys²⁷⁴	Cys³²²	Cys³²⁵ MC	Cys⁴¹⁷ MC

Pos in alignment	594	673	680	710	717	731	737	794	832	851	872	923	929	953
Subcell. location	C	C	C	C	C	C	C	C	C	C	C	C	C	C
Disulfide	no	no	no	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	helix	coil	helix	helix	coil	coil	helix	strand	helix	coil	strand	coil	coil	helix
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁵⁹⁴	Cys⁶⁷³	Cys⁶⁸⁰ MC	Cys⁷¹⁰ LC	Cys⁷¹⁷	Cys⁷³¹	Cys⁷³⁷	Cys⁷⁹⁴	Cys⁸³²	Cys⁸⁵¹	Cys⁸⁷²	Cys⁹²³	Cys⁹²⁹	Cys⁹⁵³
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys⁵⁰²	Cys⁵⁸¹	Cys⁵⁸⁸ MC	Cys⁶¹⁸ LC	Cys⁶²⁵	Cys⁶³⁹	Cys⁶⁴⁵	Cys⁷⁰²	Cys⁷⁴⁰	Cys⁷⁵⁹	Cys⁷⁸⁰	Cys⁸³¹	Cys⁸³⁷	Cys⁸⁶¹
Pos in isoform 3 CSS-Palm 4.0 PalmPred
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys⁴⁴¹	Cys⁵²⁰	Cys⁵²⁷ MC	Cys⁵⁵⁷ LC	Cys⁵⁶⁴	Cys⁵⁷⁸	Cys⁵⁸⁴	Cys⁶⁴¹	Cys⁶⁷⁹	Cys⁶⁹⁸	Cys⁷¹⁹	Cys⁷⁷⁰	Cys⁷⁷⁶	Cys⁸⁰⁰

Pos in alignment	964	967	1058	1060	1099	1141	1211	1227	1248	1340	1345	1382	1399	1431
Subcell. location	C	C	C	C	C	C	C	C	C	C	C	C	C	U
Disulfide	no	no	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	coil	coil	coil	coil	coil	helix	helix	coil	helix	helix	helix	helix	helix
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁹⁶⁴	Cys⁹⁶⁷	Cys¹⁰⁵⁸	Cys¹⁰⁶⁰	Cys¹⁰⁹⁹ MC	Cys¹¹⁴¹	Cys¹²¹¹	Cys¹²²⁷	Cys¹²⁴⁸	Cys¹³⁴⁰	Cys¹³⁴⁵	Cys¹³⁸²	Cys¹³⁹⁹
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys⁸⁷²	Cys⁸⁷⁵	Cys⁹⁶⁶	Cys⁹⁶⁸	Cys¹⁰⁰⁷ MC	Cys¹⁰⁴⁹	Cys¹¹¹⁹	Cys¹¹³⁵	Cys¹¹⁵⁶	Cys¹²⁴⁸	Cys¹²⁵³	Cys¹²⁹⁰	Cys¹³⁰⁷
Pos in isoform 3 CSS-Palm 4.0 PalmPred						Cys⁴²	Cys¹¹²	Cys¹²⁸	Cys¹⁴⁹	Cys²⁴¹	Cys²⁴⁶	Cys²⁸³	Cys³⁰⁰	Cys³³²
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys⁸¹¹	Cys⁸¹⁴	Cys⁹⁰⁵	Cys⁹⁰⁷	Cys⁹⁴⁶ MC	Cys⁹⁸⁸	Cys¹⁰⁵⁸	Cys¹⁰⁷⁴	Cys¹⁰⁹⁵	Cys¹¹⁸⁷	Cys¹¹⁹²	Cys¹²²⁹	Cys¹²⁴⁶

Pos in alignment	1510	1521	1530	1551
Subcell. location	C	C	C	C
Disulfide	no	no	no	no
PSIPRED v.4.02 prediction	helix	helix	helix	helix
UniProt secondary structure	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys¹⁵¹⁰	Cys¹⁵²¹	Cys¹⁵³⁰	Cys¹⁵⁵¹
Pos in isoform 2 CSS-Palm 4.0 PalmPred	Cys¹⁴¹⁸	Cys¹⁴²⁹	Cys¹⁴³⁸	Cys¹⁴⁵⁹
Pos in isoform 3 CSS-Palm 4.0 PalmPred	Cys³⁶⁸
Pos in isoform 4 CSS-Palm 4.0 PalmPred	Cys¹³⁵⁷	Cys¹³⁶⁸	Cys¹³⁷⁷	Cys¹³⁹⁸

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

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ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	x		HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	x		HC: 102 hits LC: 73 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	x		HC: 162 hits LC: 218 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	x		HC HC: 352 hits LC LC: 8 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	x		HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	Found	in Palm increased	Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	x		List: 436 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	x		List: 1348 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	x		List: 274 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	x		List: 226 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	x		List: 354 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	x		List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	x		List: 1353 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		List: 865 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	x		HC: 1079 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		List: 646 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		LPS treatment: 646 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	x		List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	x		List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 590 hits
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	x		List: 2929 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 370 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		List: 185 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 1869 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 600 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 687 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 399 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 464 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 199 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1093 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1040 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 4107 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 3870 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1378 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1597 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	0	0.0	NA
H. sapiens	22	38	1	2.6	NA
M. musculus	23	37	0	0.0	NA
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
S. pombe	1	1	0	0.0	NA
A. thaliana	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	0	0.0	NA
C. familiaris	1	1	0	0.0	NA

References

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Gao J et al.	2022	Proteome-wide identification of palmitoylated proteins in mouse testis.	35477839
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Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
Pradhan AJ et al.	2021	Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.	33848465
Morrison E et al.	2020	Dynamic palmitoylation events following T-cell receptor signaling.	32651440
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Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
Santiago-Tirado FH et al.	2015	A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.	25970403
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
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