SwissPalm

Protein general information from UniProt

Protein names

Palmitoyltransferase ZDHHC5, EC 2.3.1.225; Zinc finger DHHC domain-containing protein 5, DHHC-5; Zinc finger protein 375

Subcellular location

Cell membrane (By similarity); Synapse (Probable)

Isoforms & protein length

Isoform 1 (main): 715 aa; Isoform 2 : 662 aa

Known functions

Palmitoyl acyltransferase for the G-protein coupled receptor SSTR5. Also palmitoylates FLOT2 (By similarity). Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA. Palmitoyl acyltransferase for the G-protein coupled receptor SSTR5. Also palmitoylates FLOT2 (By similarity). hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-hexadecanoyl-L-cysteinyl-[protein] Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates such as CTNND2, CD36, STAT3 and S1PR1 thus plays a role in various biological processes including cell adhesion, fatty acid uptake, bacterial sensing or cardiac functions (PubMed:21820437, PubMed:29185452, PubMed:31402609). Plays an important role in the regulation of synapse efficacy by mediating palmitoylation of delta-catenin/CTNND2, thereby increasing synaptic delivery and surface stabilization of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs). Under basal conditions, remains at the synaptic membrane through FYN-mediated phosphorylation that prevents association with endocytic proteins (PubMed:26334723). Neuronal activity enhances the internalization and trafficking of DHHC5 from spines to dendritic shafts where it palmitoylates delta-catenin/CTNND2 (PubMed:26334723). Regulates cell adhesion at the plasma membrane by palmitoylating GOLGA7B and DSG2 (PubMed:31402609). Plays a role in innate immune response by mediating the palmitoylation of NOD1 and NOD2 and their proper recruitment to the bacterial entry site and phagosomes (PubMed:31649195). Participates also in fatty acid uptake by palmitoylating CD36 and thereby targeting it to the plasma membrane. Upon binding of fatty acids to CD36, gets phosphorylated by LYN leading to inactivation and subsequent CD36 caveolar endocytosis (PubMed:32958780). Controls oligodendrocyte development by catalyzing STAT3 palmitoylation (By similarity). hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-hexadecanoyl-L-cysteinyl-[protein]

Cross-references

Source	Identifiers
UniProt gene names	ZDHHC5, KIAA1748, ZNF375
UniProt secondary AC	Q2TGF0, Q6ZMF0, Q8TAK8, Q9H923, Q9UFI7
Refseq protein ID	XP_011543201.1, XP_011543202.1, XP_011543203.1, NP_056272.2, XP_005273946.1
IPI ID	IPI00216546, IPI00328272, IPI00294857

Source	Identifiers
Ensembl protein	ENSP00000287169, ENSP00000432202
Ensembl gene	ENSG00000156599
CellPalmSeq	search-zdhhcs/zdhhc5

Enzymes

PAT/APT	Enzyme	Experiments
PAT	ZDH20_HUMAN	SPalmE:1384 Plain F et al. (2020) []

Protein acyltransferase activity (PAT)

Substrate UniProt ID	Substrate description	Substrate isoform AC (site position)	Experiments
CTND1_HUMAN	Catenin delta-1	O60716	SPalmE:772 Brigidi GS et al. (2015) SPalmE:773 Brigidi GS et al. (2015)
S1PR1_HUMAN	Sphingosine 1-phosphate receptor 1, S1P receptor 1, S1P1	P21453 (328,329,331)	SPalmE:914 Badawy SMM et al. (2017)
IFM3_HUMAN	Interferon-induced transmembrane protein 3	Q01628	SPalmE:916 McMichael TM et al. (2017)
FLOT2_HUMAN	Flotillin-2	Q14254 (4,19,20)	SPalmE:924 Li Y et al. (2012)
FLOT1_HUMAN	Flotillin-1	O75955	SPalmE:1120 Howie J et al. (2014)
PLM_HUMAN	Phospholemman	O00168 (60,62)	SPalmE:1120 Howie J et al. (2014)
FURIN_HUMAN	Furin, EC 3.4.21.75	P09958 (771)	SPalmE:1211 Sergeeva OA et al. (2019) [] SPalmE:1213 Sergeeva OA et al. (2019) []
PCSK7_HUMAN	Proprotein convertase subtilisin/kexin type 7, EC 3.4.21.-	Q16549 (699,704)	SPalmE:1212 Sergeeva OA et al. (2019) [] SPalmE:1214 Sergeeva OA et al. (2019) []
CD36_HUMAN	Platelet glycoprotein 4	P16671	SPalmE:1251 Wang J et al. (2019) [] SPalmE:1390 Hao JW et al. (2020) []
KCIP2_HUMAN	Kv channel-interacting protein 2, KChIP2	Q9NS61 (46)	SPalmE:1279 Murthy A et al. (2019) []
GOG7B_HUMAN	Golgin subfamily A member 7B	Q2TAP0 (33,78,81,90,141)	SPalmE:1282 Woodley KT et al. (2019) []
DSG2_HUMAN	Desmoglein-2	Q14126	SPalmE:1282 Woodley KT et al. (2019) []
PKP3_HUMAN	Plakophilin-3	Q9Y446	SPalmE:1282 Woodley KT et al. (2019) []
CKAP4_HUMAN	Cytoskeleton-associated protein 4	Q07065 (100)	SPalmE:1307 Sada R et al. (2019) [] SPalmE:1309 Sada R et al. (2019) []
CAV1_HUMAN	Caveolin-1	Q03135	SPalmE:1323 Gadalla MR et al. (2020) []
GNAS1_RAT	Guanine nucleotide-binding protein G	Q63803	SPalmE:1342 Chen JJ et al. (2020) []
GNAI1_RAT	Guanine nucleotide-binding protein G	P10824	SPalmE:1342 Chen JJ et al. (2020) []
NAC1_HUMAN	Sodium/calcium exchanger 1	P32418 (773)	SPalmE:1376 Gök C et al. (2020) []
IL6RB_HUMAN	Interleukin-6 receptor subunit beta, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB	P40189	SPalmE:1389 Collura KM et al. (2020) [] SPalmE:1388 Collura KM et al. (2020) []

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

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UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Techniques	Sites	Predicted
Q8VDZ4	ZDHC5_MOUSE	OrthoDB, OMA, Ensembl, InParanoid, MetaPhors	M. musculus	Zdhhc5, Kiaa1748	Palmitoyltransferase ZDHHC5, EC 2.3.1.225	9/23	3	4	Yes
M9NGX0	M9NGX0_DROME	Ensembl	D. melanogaster	Zdhhc8, Dm_X:47283, anon-Pen16, CG12136, CG15372, CG17251, Dmel\CG34449, p16, Pen16, pen16, CG32705, CG34449, Dmel_CG34449	Palmitoyltransferase	0/1	0	0	Yes
M9PH36	M9PH36_DROME	Ensembl, MetaPhors	D. melanogaster	Zdhhc8, dZDHHC8, Dm_X:47283, anon-Pen16, CG12136, CG15372, CG17251, Dmel\CG34449, p16, Pen16, pen16, CG32705, CG34449, Dmel_CG34449	Zinc finger DHHC-type containing 8, isoform E	0/1	0	0	Yes
A8JUM5	A8JUM5_DROME	Ensembl	D. melanogaster	Zdhhc8, Dm_X:47283, anon-Pen16, CG12136, CG15372, CG17251, Dmel\CG34449, p16, Pen16, pen16, CG32705, CG34449, Dmel_CG34449	Palmitoyltransferase	0/1	0	0	Yes
Q9W345	ZDHC8_DROME	Ensembl	D. melanogaster	Zdhhc8, Dm_X:47283, anon-Pen16, CG12136, CG15372, CG17251, Dmel\CG34449, p16, Pen16, pen16, CG32705, CG34449, Dmel_CG34449	Palmitoyltransferase ZDHHC8, EC 2.3.1.225	0/1	0	0	Yes
O74384	ERFB_SCHPO	MetaPhors	S. pombe	erf2, mug142, SPBC3H7.09	Palmitoyltransferase erf2	0/1	0	0	Yes
Q0WQK2	ZDHC9_ARATH	MetaPhors	A. thaliana	At3g26935, MOJ10.1, PAT07	Probable protein S-acyltransferase 7, EC 2.3.1.225	0/1	0	0	No
Q9SB58	ZDH19_ARATH	MetaPhors	A. thaliana	At4g24630, F22K18.170, PAT08	Protein S-acyltransferase 8, EC 2.3.1.225	1/1	1	0	No
B3DN87	ZDH12_ARATH	MetaPhors	A. thaliana	At3g56920, T8M16_250, PAT01	Probable protein S-acyltransferase 1, EC 2.3.1.225	0/1	0	0	No
O80685	ZDHC4_ARATH	MetaPhors	A. thaliana	At2g40990, T3K9.24, PAT02	Probable protein S-acyltransferase 2, EC 2.3.1.225	0/1	0	0	Yes
Q9M306	ZDH10_ARATH	MetaPhors	A. thaliana	At3g48760, T21J18.30, PAT05	Probable protein S-acyltransferase 5, EC 2.3.1.225	0/1	0	0	No
Q2THW9	ZDHC5_CANLF	InParanoid	C. familiaris	ZDHHC5	Palmitoyltransferase ZDHHC5, EC 2.3.1.225	0/1	0	0	Yes
Q2THW7	ZDHC5_RAT	OrthoDB, OMA, Ensembl, InParanoid, MetaPhors	R. norvegicus	Zdhhc5	Palmitoyltransferase ZDHHC5, EC 2.3.1.225	1/3	2	0	Yes
E1BLT8	ZDHC5_BOVIN	OrthoDB, OMA, Ensembl, InParanoid, MetaPhors	B. taurus	ZDHHC5	Palmitoyltransferase ZDHHC5, EC 2.3.1.225	0/2	0	0	Yes
A8JUM2	A8JUM2_DROME	OrthoDB, Ensembl	D. melanogaster	Zdhhc8, Dm_X:47283, anon-Pen16, CG12136, CG15372, CG17251, Dmel\CG34449, p16, Pen16, pen16, CG32705, CG34449, Dmel_CG34449	Uncharacterized protein, isoform B	0/1	0	0	Yes
J9NYL0	J9NYL0_CANLF	OrthoDB	C. familiaris	ZDHHC8	Palmitoyltransferase	0/1	0	0	No
G1K291	G1K291_CANLF	OMA	C. familiaris	ZDHHC5	Palmitoyltransferase	0/1	0	0	Yes

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:772	Brigidi GS et al.	2015	Homo sapiens	Hippocampal neuron	Total cell lysate	ABE, Co-IP of PAT and substrate, Depletion of PAT by shRNA, NH2OH cleavage of thioester bond, PAT localization by fluorescent tag, PAT overexpression, PAT point mutation, Point mutation, Substrate localization by fluorescence tag
SPalmE:773	Brigidi GS et al.	2015	Homo sapiens	HEK293T	Total cell lysate	Depletion of PAT by shRNA, Substrate localization by fluorescence tag
SPalmE:914	Badawy SMM et al.	2017	Homo sapiens	SH-SY5Y human bone marrow neuroblastoma	Total cell lysate	2BP inhibition, ABE, Depletion of PAT by siRNA, NH2OH cleavage of thioester bond, PAT KO, PAT localization by fluorescent tag, Point mutation
SPalmE:916	McMichael TM et al.	2017	Homo sapiens	HAP1	Total cell lysate	Alk16 Labeling, Depletion of PAT by siRNA
SPalmE:924	Li Y et al.	2012	Chlorocebus aethiops	COS1	Total cell lysate	ABE
SPalmE:1120	Howie J et al.	2014	Homo sapiens	HEK293FT	Total cell lysate	ARAC, Co-IP of PAT and substrate, Depletion of PAT by siRNA, PAT overexpression, PAT truncation, Point mutation
SPalmE:1211	Sergeeva OA et al.	2019	Homo sapiens	RPE-1	Total cell lysate	ARAC, Click chemistry, Co-IP of PAT and substrate, Depletion of PAT by siRNA, NH2OH cleavage of thioester bond, Point mutation, [3H]palmitic acid labeling
SPalmE:1212	Sergeeva OA et al.	2019	Homo sapiens	RPE-1	Total cell lysate	ARAC, Click chemistry, Co-IP of PAT and substrate, Depletion of PAT by siRNA, NH2OH cleavage of thioester bond, Point mutation, [3H]palmitic acid labeling
SPalmE:1213	Sergeeva OA et al.	2019	Homo sapiens	HAP1	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, PAT KO
SPalmE:1214	Sergeeva OA et al.	2019	Homo sapiens	HAP1	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, PAT KO
SPalmE:1251	Wang J et al.	2019	Homo sapiens	HEK293T	Total membrane	ARAC, Depletion of PAT by shRNA, NH2OH cleavage of thioester bond, PAT KO
SPalmE:1279	Murthy A et al.	2019	Homo sapiens	HEK293	Plasma Membrane	15-HDYA labeling, 2BP inhibition, NH2OH cleavage of thioester bond, PAT overexpression
SPalmE:1282	Woodley KT et al.	2019	Homo sapiens	HeLa	Plasma Membrane	ABE, Depletion of PAT by siRNA, IF localization, NH2OH cleavage of thioester bond, PAT localization by IF, PAT point mutation, Point mutation
SPalmE:1307	Sada R et al.	2019	Homo sapiens	S2-CP8	Plasma Membrane	, 2BP inhibition, ABE, APE, C14N3 labeling, Click chemistry, NH2OH cleavage of thioester bond, Palmostatin B inhibition, Point mutation
SPalmE:1309	Sada R et al.	2019	Homo sapiens	HEK293T	Plasma Membrane	ABE, APE, Click chemistry, NH2OH cleavage of thioester bond, PAT overexpression, Point mutation
SPalmE:1323	Gadalla MR et al.	2019	Homo sapiens	HeLa	Viral fraction	Depletion of PAT by siRNA, NH2OH cleavage of thioester bond, [3H]palmitic acid labeling
SPalmE:1341	Chen JJ et al.	2020	Mus musculus	Ventricular myocytes	Total cell lysate	ABE, NH2OH cleavage of thioester bond
SPalmE:1342	Chen JJ et al.	2020	Rattus norvegicus	NA	NA	[3H]palmitoyl-CoA labeling
SPalmE:1376	Gök C et al.	2020	Homo sapiens	HEK293FT	Total cell lysate	, ARAC, NH2OH cleavage of thioester bond, PAT KO, PAT overexpression, Palmostatin B inhibition, Point mutation
SPalmE:1384	Plain F et al.	2020	Homo sapiens	HEK293	Total cell lysate	APE, ARAC, NH2OH cleavage of thioester bond, Point mutation
SPalmE:1388	Collura KM et al.	2020	Homo sapiens	HEK293T	Total cell lysate	2BP inhibition, ABE, NH2OH cleavage of thioester bond, PAT overexpression, PAT point mutation
SPalmE:1389	Collura KM et al.	2020	Homo sapiens	Cortical neuron	Total cell lysate	ABE, Depletion of PAT by shRNA, NH2OH cleavage of thioester bond
SPalmE:1390	Hao JW et al.	2020	Mus musculus	3T3-L1	Total cell lysate	, ARAC, NH2OH cleavage of thioester bond, PAT KO, PAT point mutation, Palmostatin B inhibition

Sites (from validation or large-scale experiments)

Isoforms	Position	Probability	Studies	Techniques
Q9C0B5	236	NA	SPalmE:578 Yang W et al. (2010)* SPalmE:724 Fang C et al. (2016)* SPalmE:1384 Plain F et al. (2020) []	ABE, APE, ARAC, NH2OH cleavage of thioester bond, Point mutation
Q9C0B5	237	NA	SPalmE:578 Yang W et al. (2010)* SPalmE:724 Fang C et al. (2016)* SPalmE:1384 Plain F et al. (2020) []	ABE, APE, ARAC, NH2OH cleavage of thioester bond, Point mutation
Q9C0B5	245	NA	SPalmE:578 Yang W et al. (2010)*	ABE
Q9C0B5	245	1.0	SPalmE:1185 Thinon E et al. (2018) []*	Alk16 Labeling, LC-MS, NH2OH cleavage of thioester bond
Q9C0B5	236	1.0	SPalmE:1185 Thinon E et al. (2018) []*	Alk16 Labeling, LC-MS, NH2OH cleavage of thioester bond
Q9C0B5	237	1.0	SPalmE:1185 Thinon E et al. (2018) []*	Alk16 Labeling, LC-MS, NH2OH cleavage of thioester bond
Q9C0B5	134	NA	SPalmE:1384 Plain F et al. (2020) []	APE, ARAC, NH2OH cleavage of thioester bond, Point mutation

Cysteine summary and predictions

Pos in alignment	36	106	109	117	120	123	126	134	140	191	236	237	245
Subcell. location	E	C	C	C	C	C	C	C	C	E	C	C	C
Disulfide	no	no	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	helix	coil	coil	coil	coil	coil	strand	coil	coil	helix	helix	helix	coil
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys³⁶	Cys¹⁰⁶	Cys¹⁰⁹	Cys¹¹⁷	Cys¹²⁰	Cys¹²³	Cys¹²⁶	Cys¹³⁴	Cys¹⁴⁰	Cys¹⁹¹	Cys²³⁶ HC	Cys²³⁷ HC	Cys²⁴⁵
Pos in isoform 2 CSS-Palm 4.0 PalmPred		Cys⁵³	Cys⁵⁶	Cys⁶⁴	Cys⁶⁷	Cys⁷⁰	Cys⁷³	Cys⁸¹	Cys⁸⁷	Cys¹³⁸	Cys¹⁸³ HC	Cys¹⁸⁴ HC	Cys¹⁹²

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

Tip: Keep CTRL key pressed down while clicking on column headers to do multiple sorting. This applies to any sortable table on this site.

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	Found	in LC	HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	Found	in HC	HC: 162 hits LC: 218 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	Found	in HC	HC: 102 hits LC: 73 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	x		HC HC: 352 hits LC LC: 8 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	Ortholog	in LC [ZDH19]	HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	x		List: 436 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	x		Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	x		List: 354 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Found	in List	List: 1348 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	x		List: 354 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	Found	in List	List: 226 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	Found	in List	List: 274 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	Found	in List	List: 100 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	x		List: 1353 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	Ortholog	in HC [ZDHC5]	HC: 1079 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	Ortholog	in List [ZDHC5]	List: 865 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	Found	in HC	HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Ortholog	in HC [ZDHC5]	HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	x		List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	x		List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in LPS treatment [ZDHC5]	LPS treatment: 646 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in List [ZDHC5]	List: 646 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	Ortholog	in Palm. up with LPS [ZDHC5]	Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	Found	in Chymotrypsin	Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	Ortholog	in HC [ZDHC5]	HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	Found	in List	List: 2929 hits
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	Found	in List	List: 590 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [ZDHC5]	List: 370 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 600 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		List: 185 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1869 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [ZDHC5]	List: 1093 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 687 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Found	in List	List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	Found	in List	List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 199 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [ZDHC5]	List: 399 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [ZDHC5]	List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [ZDHC5]	List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [ZDHC5]	List: 1040 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [ZDHC5]	HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 464 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [ZDHC5]	HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [ZDHC5]	HC: 4107 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1597 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in List [ZDHC5]	List: 1378 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	Ortholog	in HC [ZDHC5]	HC: 3870 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	1	25.0	ZDHC5_RAT
H. sapiens	22	38	17	44.7	H0YEF4_HUMAN
M. musculus	23	37	17	45.9	ZDHC5_MOUSE
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
A. thaliana	1	1	1	100.0	ZDH19_ARATH
S. pombe	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	0	0.0	M9NGX0_DROME , A8JUM5_DROME , A8JUM2_DROME , ZDHC8_DROME , M9PH36_DROME
C. familiaris	1	1	0	0.0	NA

References

Authors	Year	Reference title	PMID
Mariscal J et al.	2020	Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles.	32944167
Marin EP et al.	2012	Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases.	22496122
Thinon E et al.	2018	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.	29575903
Peng T et al.	2015	Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.	25575299
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Ziemlińska E et al.	2021	Palm Oil-Rich Diet Affects Murine Liver Proteome and <i>S</i>-Palmitoylome.	34884899
Sada R et al.	2019	Dynamic palmitoylation controls the microdomain localization of the DKK1 receptors CKAP4 and LRP6.	31744930
Ivaldi C et al.	2012	Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.	22615937
Colquhoun DR et al.	2015	Bioorthogonal mimetics of palmitoyl-CoA and myristoyl-CoA and their subsequent isolation by click chemistry and characterization by mass spectrometry reveal novel acylated host-proteins modified by HIV-1 infection.	25914232
Gould NS et al.	2015	Site-Specific Proteomic Mapping Identifies Selectively Modified Regulatory Cysteine Residues in Functionally Distinct Protein Networks.	26165157
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
Ren W et al.	2013	Proteomic analysis of protein palmitoylation in adipocytes.	23599907
Ripamonti M et al.	2021	Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions.	33782527
Morrison E et al.	2020	Dynamic palmitoylation events following T-cell receptor signaling.	32651440
Won SJ et al.	2018	Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.	29733200
Plain F et al.	2020	Control of protein palmitoylation by regulating substrate recruitment to a zDHHC-protein acyltransferase.	32737405
Murthy A et al.	2019	Dynamic palmitoylation regulates trafficking of K channel interacting protein 2 (KChIP2) across multiple subcellular compartments in cardiac myocytes.	31362018
McMichael TM et al.	2017	The palmitoyltransferase ZDHHC20 enhances interferon-induced transmembrane protein 3 (IFITM3) palmitoylation and antiviral activity.	29079573
Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Emmer BT et al.	2011	Global analysis of protein palmitoylation in African trypanosomes.	21193548
Zhou B et al.	2019	Low-background Acyl-biotinyl Exchange Largely Eliminates the Co-isolation of Non-S-acylated Proteins and Enables Deep S-acylproteomic Analysis.	31251020
Martin BR et al.	2009	Large-scale profiling of protein palmitoylation in mammalian cells.	19137006
Martin BR et al.	2011	Global profiling of dynamic protein palmitoylation.	22056678
Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
Gadalla MR et al.	2019	Hemagglutinin of Influenza A, but not of Influenza B and C viruses is acylated by ZDHHC2, 8, 15 and 20.	31872235
Wang J et al.	2019	DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane.	30605677
Pinner AL et al.	2016	Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia.	26876311
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Morrison E et al.	2015	Quantitative analysis of the human T cell palmitome.	26111759
Howie J et al.	2014	Substrate recognition by the cell surface palmitoyl transferase DHHC5.	25422474
Wan J et al.	2013	Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease.	24211138
Merrick BA et al.	2011	Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3.	21785166
Thinon E et al.	2016	Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.	27350074
Miles MR et al.	2021	Global identification of S-palmitoylated proteins and detection of palmitoylating (DHHC) enzymes in heart.	33636221
Zhang X et al.	2018	Ultradeep Palmitoylomics Enabled by Dithiodipyridine-Functionalized Magnetic Nanoparticles.	29660268
Caballero MC et al.	2016	Identification of new palmitoylated proteins in Toxoplasma gondii.	26825284
Hernandez JL et al.	2016	Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.	27030425
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
Sergeeva OA et al.	2019	Anthrax toxin requires ZDHHC5-mediated palmitoylation of its surface-processing host enzymes.	30610172
Badawy SMM et al.	2017	DHHC5-mediated palmitoylation of S1P receptor subtype 1 determines G-protein coupling.	29185452
Sobocińska J et al.	2018	Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.	29217618
Zaręba-Kozioł M et al.	2019	Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins.	31311849
Woodley KT et al.	2019	S-acylated Golga7b stabilises DHHC5 at the plasma membrane to regulate cell adhesion.	31402609
Hemsley PA et al.	2013	A proteomic approach identifies many novel palmitoylated proteins in Arabidopsis.	23252521
Chesarino NM et al.	2014	Chemoproteomics reveals Toll-like receptor fatty acylation.	25371237
Zhang MM et al.	2013	Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.	23843742
Wegleiter T et al.	2019	Palmitoylation of BMPR1a regulates neural stem cell fate.	31772009
Pradhan AJ et al.	2021	Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.	33848465
Collura KM et al.	2020	The palmitoyl acyltransferases ZDHHC5 and ZDHHC8 are uniquely present in DRG axons and control retrograde signaling via the Gp130/JAK/STAT3 pathway.	32958558
Roth AF et al.	2006	Global analysis of protein palmitoylation in yeast.	16751107
Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
Strassburger K et al.	2019	Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.	30735487
Hao JW et al.	2020	CD36 facilitates fatty acid uptake by dynamic palmitoylation-regulated endocytosis.	32958780
Zaręba-Kozioł M et al.	2021	S-Palmitoylation of Synaptic Proteins as a Novel Mechanism Underlying Sex-Dependent Differences in Neuronal Plasticity.	34200797
Uzbekova S et al.	2021	Protein Palmitoylation in Bovine Ovarian Follicle.	34769186
Dumoulin A et al.	2018	S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.	30319353
Gorenberg EL et al.	2022	Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.	35358180
Fang C et al.	2016	Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody.	26865113
Chen JJ et al.	2020	DHHC5 Mediates β-Adrenergic Signaling in Cardiomyocytes by Targeting Gα Proteins.	31547976
Upadhyay S et al.	2016	Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation.	27879337
Serwa RA et al.	2015	Systems Analysis of Protein Fatty Acylation in Herpes Simplex Virus-Infected Cells Using Chemical Proteomics.	26256475
Foe IT et al.	2015	Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.	26468752
Santiago-Tirado FH et al.	2015	A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.	25970403
Dowal L et al.	2011	Proteomic analysis of palmitoylated platelet proteins.	21813449
Wei X et al.	2014	Insulin-regulated protein palmitoylation impacts endothelial cell function.	24357059
Gök C et al.	2020	Dynamic Palmitoylation of the Sodium-Calcium Exchanger Modulates Its Structure, Affinity for Lipid-Ordered Domains, and Inhibition by XIP.	32521252
Forrester MT et al.	2011	Site-specific analysis of protein S-acylation by resin-assisted capture.	21044946
Yang W et al.	2010	Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes.	19801377
Jones ML et al.	2012	Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis.	22901544
Brigidi GS et al.	2015	Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5.	26334723
Kang R et al.	2008	Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.	19092927
Gao J et al.	2022	Proteome-wide identification of palmitoylated proteins in mouse testis.	35477839

Q9C0B5 ZDHC5_HUMAN