SwissPalm

Q9QY30 ABCBB_MOUSE

General info Cross-references Enzymes Sequences Orthologs Ortholog sequences Predictions Palmitoyl-proteomes References

Palmitoyl-proteomes

2/23 articles for mouse
2 orthologs

Palmitoyl-proteome experiments

Validation experiments

0 experiment
0 ortholog

Curated sites & Enzymes

0 site from validation experiments^*
0 site from palmitoyl-proteomes^*
DHHC-PATs : ZDH13_MOUSE
No APT known
^*In main isoform only

Predictions

Predicted

1 cysteine in main isoform

Predictions

Protein general information from UniProt

Protein names

Bile salt export pump, EC 7.6.2.-; ATP-binding cassette sub-family B member 11; Sister of P-glycoprotein

Subcellular location

Membrane (Probable); Apical cell membrane (Potential); Recycling endosome membrane (Potential); Endosome (Potential); Cell membrane (Potential)

Isoforms & protein length

Isoform 1 (main): 1321 aa

Known functions

Internalized at the canalicular membrane through interaction with the adapter protein complex 2 (AP-2). At steady state, localizes in the canalicular membrane but is also present in recycling endosomes. ABCB11 constantly and rapidly exchanges between the two sites through tubulo-vesicles carriers that move along microtubules. Microtubule-dependent trafficking of ABCB11 is enhanced by taurocholate and cAMP and regulated by STK11 through a PKA-mediated pathway. Trafficking of newly synthesized ABCB11 through endosomal compartment to the bile canalicular membrane is accelerated by cAMP but not by taurocholate (By similarity). Cell membrane expression is up-regulated by short- and medium-chain fatty acids (By similarity). Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ. Multifunctional polypeptide with two homologous halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. N-glycosylated. Ubiquitinated; short-chain ubiquitination regulates cell-Surface expression of ABCB11. Transgenic mice with up-regulated liver canalicular membrane expression of Abcb11 appear healthy and normal and demonstrate any difference in longevity. Transgenic mice exhibit a normal reproductive rate and gender distribution and are born in a normal Mendelian distribution. Transgenic mice have food consumption identical to their background strain controls. Transgenic mice manifest increases of both bile flow and biliary lipid secretion and are resistant to the development of hepatic steatosis (PubMed:14570929). Homozygous Abcb11 knockout mice on a mixed genetic background are viable and fertile, but displayed growth retardation. Their body weight is about 20% lower than their wild-type littermates at weaning (21 days after birth). They tend to have a lower body weight throughout their life, but display only mild non progressive cholestasis (PubMed:11172067). Homozygous Abcb11 knockout mice on a pure C57BL/6J background exhibit a progressive intrahepatic cholestasis due to an hepatic bile acid retention and an alteration of lipid metabolism (PubMed:22619174). Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Interacts with HAX1 (By similarity). Interacts with the adapter protein complex 2 (AP-2) throught AP2A2 or AP2A1; this interaction regulates cell membrane expression of ABCB11 through its internalization in a clathrin-dependent manner and its subsequent degradation (By similarity). Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner (PubMed:14570929, PubMed:11172067, PubMed:23764895, PubMed:22619174, PubMed:19228692). Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts (By similarity). Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion (By similarity). ATP + cholate(in) + H2O = ADP + cholate(out) + H(+) + phosphate ATP + H2O + taurocholate(in) = ADP + H(+) + phosphate + taurocholate(out) ATP + glycocholate(in) + H2O = ADP + glycocholate(out) + H(+) + phosphate ATP + glycochenodeoxycholate(in) + H2O = ADP + glycochenodeoxycholate(out) + H(+) + phosphate ATP + H2O + taurochenodeoxycholate(in) = ADP + H(+) + phosphate + taurochenodeoxycholate(out) ATP + glycoursodeoxycholate(in) + H2O = ADP + glycoursodeoxycholate(out) + H(+) + phosphate ATP + H2O + tauroursodeoxycholate(in) = ADP + H(+) + phosphate + tauroursodeoxycholate(out) ATP + H2O + taurodeoxycholate(in) = ADP + H(+) + phosphate + taurodeoxycholate(out) ATP + H2O + taurolithocholate 3-sulfate(in) = ADP + H(+) + phosphate + taurolithocholate 3-sulfate(out) ATP + H2O + pravastatin(in) = ADP + H(+) + phosphate + pravastatin(out) The uptake of taurocholate is inhibited by taurolithocholate sulfate with an IC(50) of 9 uM. Pravastatin competitively inhibits the transport of taurocholic acid. Cyclosporin A, glibenclamide, rifampicin and troglitazonestrongly competitively inhibit the transport activity of taurocholate (By similarity). The canalicular transport activity of taurocholate is strongly dependent on canalicular membrane cholesterol content (By similarity) (PubMed:19228692). The uptake of taurocholate is increased by short- and medium-chain fatty acids. Cholesterol increases transport capacity of taurocholate without affecting the affinity for the substrate (By similarity). Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Interacts with HAX1. Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ. Multifunctional polypeptide with two homologous halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Interacts with HAX1. Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ. Multifunctional polypeptide with two homologous halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Cross-references

Source	Identifiers
UniProt gene names	Abcb11, Bsep, Spgp
UniProt secondary AC	A2AUN4, Q9QZE8
Refseq protein ID	NP_066302.2, XP_006499732.1
IPI ID	IPI00622875, IPI00828714, IPI00135511

Source	Identifiers
Ensembl protein	ENSMUSP00000099770, ENSMUSP00000099771
Ensembl gene	ENSMUSG00000027048
MGI ID	MGI:1351619

Enzymes

PAT/APT	Enzyme	Experiments
PAT	ZDH13_MOUSE	SPalmE:855 Shen LF et al. (2017)*

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Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

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UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Techniques	Predicted
Q7K6A5	Q7K6A5_PLAF7	OMA, InParanoid	P. falciparum	PF3D7_0523000	Multidrug resistance protein 1	1/1	2	Yes
Q00748	MDR65_DROME	Ensembl, MetaPhors	D. melanogaster	Mdr65, CG10181	Multidrug resistance protein homolog 65, EC 7.6.2.2	0/1	0	Yes
Q5BI62	Q5BI62_DROME	InParanoid, MetaPhors	D. melanogaster	Dm2, Dm3, Dmel\CG8523, MDR50, mdr50, Mdr50, CG8523, Dmel_CG8523	LP14331p	0/1	0	No
Q9M1Q9	AB21B_ARATH	InParanoid, MetaPhors, OMA	A. thaliana	ABCB21, MDR17, PGP21, At3g62150, T17J13.110	ABC transporter B family member 21, ABC transporter ABCB.21, AtABCB21	0/1	0	No
Q9FWX7	AB11B_ARATH	InParanoid, MetaPhors	A. thaliana	ABCB11, MDR8, PGP11, At1g02520, T14P4.15	ABC transporter B family member 11, ABC transporter ABCB.11, AtABCB11	0/1	0	No
Q9FWX8	AB12B_ARATH	InParanoid, MetaPhors	A. thaliana	ABCB12, MDR16, PGP12, At1g02530, T14P4.14	ABC transporter B family member 12, ABC transporter ABCB.12, AtABCB12	0/1	0	No
Q9SYI2	AB3B_ARATH	InParanoid, MetaPhors	A. thaliana	ABCB3, MDR3, PGP3, At4g01820, T7B11.8	ABC transporter B family member 3, ABC transporter ABCB.3, AtABCB3	0/1	0	No
Q9SYI3	AB5B_ARATH	InParanoid, MetaPhors	A. thaliana	ABCB5, MDR5, PGP5, At4g01830, T7B11.9	ABC transporter B family member 5, ABC transporter ABCB.5, AtABCB5	0/1	0	Yes
Q9M0M2	AB9B_ARATH	InParanoid, MetaPhors	A. thaliana	ABCB9, MDR9, PGP9, At4g18050, F15J5.20	ABC transporter B family member 9, ABC transporter ABCB.9, AtABCB9	0/1	0	No
Q9FHF1	AB7B_ARATH	InParanoid, MetaPhors	A. thaliana	ABCB7, MDR7, PGP7, At5g46540, K11I1.13	ABC transporter B family member 7, ABC transporter ABCB.7, AtABCB7	0/1	0	No
Q8LPK2	AB2B_ARATH	InParanoid, MetaPhors	A. thaliana	ABCB2, MDR2, PGP2, At4g25960, F20B18.70	ABC transporter B family member 2, ABC transporter ABCB.2, AtABCB2	0/1	0	Yes
Q9VRW2	Q9VRW2_DROME	MetaPhors	D. melanogaster	Dmel\CG10226, AMT, CG10226, Dmel_CG10226	Uncharacterized protein, isoform A	0/1	0	No
Q9ZR72	AB1B_ARATH	MetaPhors	A. thaliana	ABCB1, MDR1, PGP1, At2g36910, T1J8.9	ABC transporter B family member 1, ABC transporter ABCB.1, AtABCB1	0/1	0	Yes
Q9M3B9	AB20B_ARATH	MetaPhors	A. thaliana	ABCB20, MDR14, PGP20, At3g55320, T26I12.200	ABC transporter B family member 20, ABC transporter ABCB.20, AtABCB20	0/1	0	Yes
Q9C7F8	AB13B_ARATH	MetaPhors	A. thaliana	ABCB13, MDR15, PGP13, At1g27940, F13K9.5	ABC transporter B family member 13, ABC transporter ABCB.13, AtABCB13	0/1	0	No
Q9LSJ8	AB16B_ARATH	MetaPhors	A. thaliana	ABCB16, MDR18, PGP16, At3g28360, MFJ20.4	ABC transporter B family member 16, ABC transporter ABCB.16, AtABCB16	0/1	0	No
Q9LSJ5	AB18B_ARATH	MetaPhors	A. thaliana	ABCB18, MDR20, PGP18, At3g28390, MFJ20.7	ABC transporter B family member 18, ABC transporter ABCB.18, AtABCB18	0/1	0	Yes
Q9LSJ6	AB17B_ARATH	MetaPhors	A. thaliana	ABCB17, MDR19, PGP17, At3g28380, MFJ20.6	ABC transporter B family member 17, ABC transporter ABCB.17, AtABCB17	0/1	0	No
Q9SGY1	AB10B_ARATH	MetaPhors	A. thaliana	ABCB10, MDR10, PGP10, At1g10680, F20B24.12	ABC transporter B family member 10, ABC transporter ABCB.10, AtABCB10	0/1	0	Yes
Q8LPT1	AB6B_ARATH	MetaPhors	A. thaliana	ABCB6, MDR6, PGP6, At2g39480, F12L6.14	ABC transporter B family member 6, ABC transporter ABCB.6, AtABCB6	0/1	0	Yes
Q9C7F2	AB14B_ARATH	MetaPhors	A. thaliana	ABCB14, MDR12, PGP14, At1g28010, F13K9.11	ABC transporter B family member 14, ABC transporter ABCB.14, AtABCB14	0/1	0	No
Q9LHD1	AB15B_ARATH	MetaPhors	A. thaliana	ABCB15, MDR13, PGP15, At3g28345, MZF16.16	ABC transporter B family member 15, ABC transporter ABCB.15, AtABCB15	0/1	0	No
Q9LSJ2	AB22B_ARATH	MetaPhors	A. thaliana	ABCB22, MDR21, PGP22, At3g28415, MFJ20.11	ABC transporter B family member 22, ABC transporter ABCB.22, AtABCB22	0/1	0	Yes
O95342	ABCBB_HUMAN	InParanoid, OMA, MetaPhors	H. sapiens	ABCB11, BSEP	Bile salt export pump, EC 7.6.2.-	0/22	0	Yes
O80725	AB4B_ARATH	MetaPhors, InParanoid	A. thaliana	ABCB4, MDR4, PGP4, At2g47000, F14M4.17	ABC transporter B family member 4, ABC transporter ABCB.4, AtABCB4	1/1	1	No
F1PCF2	F1PCF2_CANLF	OrthoDB, OMA, InParanoid	C. familiaris	ABCB11	ATP binding cassette subfamily B member 11	0/1	0	Yes
E1BGI0	E1BGI0_BOVIN	OrthoDB, OMA, InParanoid, MetaPhors	B. taurus	ABCB11	Bile salt export pump	0/2	0	Yes
Q00449	MDR49_DROME	OrthoDB, MetaPhors	D. melanogaster	Mdr49, CG3879	Multidrug resistance protein homolog 49, EC 7.6.2.2	0/1	0	No
Q9QYJ4	ABCB9_RAT	OrthoDB	R. norvegicus	Abcb9	ABC-type oligopeptide transporter ABCB9, EC 7.4.2.6	0/3	0	Yes
P21439	MDR3_HUMAN	OrthoDB	H. sapiens	ABCB4, MDR3, PGY3	Phosphatidylcholine translocator ABCB4, EC 7.6.2.1	0/22	0	No
Q9LJX0	AB19B_ARATH	OMA, InParanoid, MetaPhors	A. thaliana	ABCB19, MDR1, MDR11, PGP19, At3g28860, MLD15.2	ABC transporter B family member 19, ABC transporter ABCB.19, AtABCB19	0/1	0	No
P36619	PMD1_SCHPO	OMA, InParanoid, MetaPhors	S. pombe	pmd1, SPCC663.03	Leptomycin B resistance protein pmd1	0/1	0	No
Q4FCM1	Q4FCM1_TOXGO	InParanoid, OMA	T. gondii	ABC.B2	ATP-binding cassette protein subfamily B member 2	0/2	0	No
O70127	ABCBB_RAT	InParanoid	R. norvegicus	Abcb11, Bsep, Spgp	Bile salt export pump, EC 7.6.2.-	0/3	0	Yes
P12866	STE6_YEAST	InParanoid	S. cerevisiae S288c	STE6, YKL209C	Alpha-factor-transporting ATPase, EC 7.4.2.7	0/1	0	Yes

Ortholog sequence alignment (CLUSTALW)

Cysteine summary and predictions

Pos in alignment	68	107	129	192	331	336	365	379	387	405	475	761	799	807
Subcell. location	T	E	E	C	T	T	T	C	C	C	C	T	T	T
Disulfide	no	no	no	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	helix	helix	coil	coil	helix	helix	helix	helix	helix	coil	coil	helix	helix	helix
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁶⁸	Cys¹⁰⁷	Cys¹²⁹	Cys¹⁹² HC	Cys³³¹	Cys³³⁶	Cys³⁶⁵ MC	Cys³⁷⁹	Cys³⁸⁷	Cys⁴⁰⁵	Cys⁴⁷⁵	Cys⁷⁶¹	Cys⁷⁹⁹	Cys⁸⁰⁷

Pos in alignment	984	1083	1117	1168	1270
Subcell. location	T	C	C	C	C
Disulfide	no	no	no	no	no
PSIPRED v.4.02 prediction	helix	strand	coil	coil	strand
UniProt secondary structure	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys⁹⁸⁴	Cys¹⁰⁸³	Cys¹¹¹⁷	Cys¹¹⁶⁸	Cys¹²⁷⁰

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

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ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	x		HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	x		HC: 162 hits LC: 218 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	x		HC: 102 hits LC: 73 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	x		HC HC: 352 hits LC LC: 8 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	Ortholog	in HC [Q7K6A5]	HC: 79 hits LC: 89 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	Ortholog	in LC [Q7K6A5]	HC: 100 hits LC: 217 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	Ortholog	in LC [AB4B]	HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	x		List: 436 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	x		Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	x		List: 354 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	x		List: 1348 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	x		List: 354 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	x		List: 226 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	x		List: 274 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	x		List: 1353 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	x		HC: 1079 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	Found	in	List: 865 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	x		List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	x		List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		LPS treatment: 646 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		List: 646 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	x		List: 2929 hits
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 590 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 370 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 600 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		List: 185 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 1869 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1093 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 687 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 199 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 399 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1040 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 464 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	Found	in HC	HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 4107 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1597 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1378 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 3870 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	0	0.0	NA
H. sapiens	22	38	0	0.0	NA
M. musculus	23	37	2	5.4	NA
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	2	100.0	Q7K6A5_PLAF7
A. thaliana	1	1	1	100.0	AB4B_ARATH
S. pombe	1	1	0	0.0	NA
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	0	0.0	MDR65_DROME
C. familiaris	1	1	0	0.0	NA

References

Authors	Year	Reference title	PMID
Kang R et al.	2008	Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.	19092927
Ren W et al.	2013	Proteomic analysis of protein palmitoylation in adipocytes.	23599907
Marin EP et al.	2012	Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases.	22496122
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
Martin BR et al.	2011	Global profiling of dynamic protein palmitoylation.	22056678
Dowal L et al.	2011	Proteomic analysis of palmitoylated platelet proteins.	21813449
Merrick BA et al.	2011	Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3.	21785166
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
Forrester MT et al.	2011	Site-specific analysis of protein S-acylation by resin-assisted capture.	21044946
Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
Yang W et al.	2010	Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes.	19801377
Martin BR et al.	2009	Large-scale profiling of protein palmitoylation in mammalian cells.	19137006
Roth AF et al.	2006	Global analysis of protein palmitoylation in yeast.	16751107
Hemsley PA et al.	2013	A proteomic approach identifies many novel palmitoylated proteins in Arabidopsis.	23252521
Ivaldi C et al.	2012	Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.	22615937
Wan J et al.	2013	Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease.	24211138
Zhang MM et al.	2013	Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.	23843742
Jones ML et al.	2012	Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis.	22901544
Wei X et al.	2014	Insulin-regulated protein palmitoylation impacts endothelial cell function.	24357059
Serwa RA et al.	2015	Systems Analysis of Protein Fatty Acylation in Herpes Simplex Virus-Infected Cells Using Chemical Proteomics.	26256475
Morrison E et al.	2015	Quantitative analysis of the human T cell palmitome.	26111759
Chesarino NM et al.	2014	Chemoproteomics reveals Toll-like receptor fatty acylation.	25371237
Santiago-Tirado FH et al.	2015	A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.	25970403
Emmer BT et al.	2011	Global analysis of protein palmitoylation in African trypanosomes.	21193548
Colquhoun DR et al.	2015	Bioorthogonal mimetics of palmitoyl-CoA and myristoyl-CoA and their subsequent isolation by click chemistry and characterization by mass spectrometry reveal novel acylated host-proteins modified by HIV-1 infection.	25914232
Foe IT et al.	2015	Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.	26468752
Gould NS et al.	2015	Site-Specific Proteomic Mapping Identifies Selectively Modified Regulatory Cysteine Residues in Functionally Distinct Protein Networks.	26165157
Peng T et al.	2015	Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.	25575299
Pinner AL et al.	2016	Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia.	26876311
Hernandez JL et al.	2016	Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.	27030425
Fang C et al.	2016	Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody.	26865113
Caballero MC et al.	2016	Identification of new palmitoylated proteins in Toxoplasma gondii.	26825284
Upadhyay S et al.	2016	Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation.	27879337
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
Sobocińska J et al.	2018	Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.	29217618
Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Thinon E et al.	2016	Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.	27350074
Thinon E et al.	2018	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.	29575903
Zhang X et al.	2018	Ultradeep Palmitoylomics Enabled by Dithiodipyridine-Functionalized Magnetic Nanoparticles.	29660268
Won SJ et al.	2018	Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.	29733200
Dumoulin A et al.	2018	S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.	30319353
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Wang J et al.	2019	DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane.	30605677
Strassburger K et al.	2019	Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.	30735487
Zhou B et al.	2019	Low-background Acyl-biotinyl Exchange Largely Eliminates the Co-isolation of Non-S-acylated Proteins and Enables Deep S-acylproteomic Analysis.	31251020
Zaręba-Kozioł M et al.	2019	Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins.	31311849
Wegleiter T et al.	2019	Palmitoylation of BMPR1a regulates neural stem cell fate.	31772009
Morrison E et al.	2020	Dynamic palmitoylation events following T-cell receptor signaling.	32651440
Mariscal J et al.	2020	Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles.	32944167
Gorenberg EL et al.	2022	Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.	35358180
Uzbekova S et al.	2021	Protein Palmitoylation in Bovine Ovarian Follicle.	34769186
Miles MR et al.	2021	Global identification of S-palmitoylated proteins and detection of palmitoylating (DHHC) enzymes in heart.	33636221
Pradhan AJ et al.	2021	Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.	33848465
Ripamonti M et al.	2021	Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions.	33782527
Zaręba-Kozioł M et al.	2021	S-Palmitoylation of Synaptic Proteins as a Novel Mechanism Underlying Sex-Dependent Differences in Neuronal Plasticity.	34200797
Ziemlińska E et al.	2021	Palm Oil-Rich Diet Affects Murine Liver Proteome and <i>S</i>-Palmitoylome.	34884899
Gao J et al.	2022	Proteome-wide identification of palmitoylated proteins in mouse testis.	35477839