SwissPalm

Protein general information from UniProt

Protein names

Palmitoyltransferase ZDHHC2, EC 2.3.1.225; Acyltransferase ZDHHC2, EC 2.3.1.-; Reduced expression associated with metastasis protein, Ream; Reduced expression in cancer protein, Rec; Zinc finger DHHC domain-containing protein 2, DHHC-2; Zinc finger protein 372

Subcellular location

Golgi apparatus membrane; Endoplasmic reticulum membrane; Cell membrane; Postsynaptic recycling endosome membrane (Potential); Postsynaptic density (Potential); Membrane (By similarity)

Isoforms & protein length

Isoform 1 (main): 367 aa

Known functions

Palmitoyltransferase specific for GAP43 and DLG4/PSD95. hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-hexadecanoyl-L-cysteinyl-[protein] Ubiquitously expressed. Reduced expression in colorectal cancers with liver metastasis. The DHHC domain is required for palmitoyltransferase activity. Belongs to the DHHC palmitoyltransferase family. Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates and is involved in a variety of cellular processes (PubMed:18508921, PubMed:18296695, PubMed:19144824, PubMed:21343290, PubMed:22034844, PubMed:23793055). Has no stringent fatty acid selectivity and in addition to palmitate can also transfer onto target proteins myristate from tetradecanoyl-CoA and stearate from octadecanoyl-CoA (By similarity). In the nervous system, plays a role in long term synaptic potentiation by palmitoylating AKAP5 through which it regulates protein trafficking from the dendritic recycling endosomes to the plasma membrane and controls both structural and functional plasticity at excitatory synapses (By similarity). In dendrites, mediates the palmitoylation of DLG4 when synaptic activity decreases and induces synaptic clustering of DLG4 and associated AMPA-type glutamate receptors (By similarity). Also mediates the de novo and turnover palmitoylation of RGS7BP, a shuttle for Gi/o-specific GTPase-activating proteins/GAPs, promoting its localization to the plasma membrane in response to the activation of G protein-coupled receptors. Through the localization of these GTPase-activating proteins/GAPs, it also probably plays a role in G protein-coupled receptors signaling in neurons (By similarity). Also probably plays a role in cell adhesion by palmitoylating CD9 and CD151 to regulate their expression and function (PubMed:18508921). Palmitoylates the endoplasmic reticulum protein CKAP4 and regulates its localization to the plasma membrane (PubMed:18296695, PubMed:19144824). Could also palmitoylate LCK and regulate its localization to the plasma membrane (PubMed:22034844). (Microbial infection) Promotes Chikungunya virus (CHIKV) replication by mediating viral nsp1 palmitoylation. hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-hexadecanoyl-L-cysteinyl-[protein] L-cysteinyl-[protein] + tetradecanoyl-CoA = CoA + S-tetradecanoyl-L-cysteinyl-[protein] L-cysteinyl-[protein] + octadecanoyl-CoA = CoA + S-octadecanoyl-L-cysteinyl-[protein] Monomer (PubMed:23793055). Homodimer (PubMed:23793055). The monomeric form has a higher catalytic activity (PubMed:23793055). Translocates to postsynaptic density when synaptic activity decreases. Ubiquitously expressed (PubMed:10918388, PubMed:22034844). Reduced expression in colorectal cancers with liver metastasis (PubMed:10918388). The DHHC domain is required for palmitoyltransferase activity. Autopalmitoylated. Belongs to the DHHC palmitoyltransferase family. Palmitoyltransferase specific for GAP43 and DLG4/PSD95. Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA. Ubiquitously expressed. Reduced expression in colorectal cancers with liver metastasis. The DHHC domain is required for palmitoyltransferase activity. Belongs to the DHHC palmitoyltransferase family.

Cross-references

Source	Identifiers
UniProt gene names	ZNF372, REC, REAM, ZDHHC2
UniProt secondary AC	D3DSP5
Refseq protein ID	NP_057437.1
IPI ID	IPI00105482, IPI00008896

Source	Identifiers
Ensembl protein	ENSP00000262096
Ensembl gene	ENSG00000104219
CellPalmSeq	search-zdhhcs/zdhhc2

Protein acyltransferase activity (PAT)

Substrate UniProt ID	Substrate description	Substrate isoform AC (site position)	Experiments
NDEL1_HUMAN	Nuclear distribution protein nudE-like 1, Protein Nudel	Q9GZM8 (273)	SPalmE:67 Shmueli A et al. (2010)
CD9_HUMAN	CD9 antigen	P21926	SPalmE:149 Sharma C et al. (2008) SPalmE:150 Sharma C et al. (2008)
CD151_HUMAN	CD151 antigen	P48509	SPalmE:149 Sharma C et al. (2008) SPalmE:150 Sharma C et al. (2008)
FYN_HUMAN	Tyrosine-protein kinase Fyn, EC 2.7.10.2	P06241	SPalmE:148 Mill P et al. (2009)
CKAP4_HUMAN	Cytoskeleton-associated protein 4	Q07065 (100)	SPalmE:154 Zhang J et al. (2008) SPalmE:999 Planey SL et al. (2009) SPalmE:1000 Planey SL et al. (2009) SPalmE:1308 Sada R et al. (2019) [] SPalmE:1405 Harada T et al. (2020) []
ABCA1_HUMAN	Phospholipid-transporting ATPase ABCA1, EC 7.6.2.1	O95477	SPalmE:161 Singaraja RR et al. (2009)
AMFR_MOUSE	E3 ubiquitin-protein ligase AMFR, EC 2.3.2.36	Q9R049-2	SPalmE:197 Fairbank M et al. (2012)
NDE1_HUMAN	Nuclear distribution protein nudE homolog 1, NudE	Q9NXR1 (274)	SPalmE:67 Shmueli A et al. (2010)
NOS3_HUMAN	Nitric oxide synthase, endothelial, EC 1.14.13.39	P29474	SPalmE:141 Fernández-Hernando C et al. (2006)
LCK_HUMAN	Tyrosine-protein kinase Lck, EC 2.7.10.2	P06239	SPalmE:305 Zeidman R et al. (2011) SPalmE:1088 Zeidman R et al. (2011)
AKAP5_HUMAN	A-kinase anchor protein 5, AKAP-5	P24588 P24588-1	SPalmE:410 Woolfrey KM et al. (2015)
SC6A3_RAT	Sodium-dependent dopamine transporter, DA transporter, DAT	P23977 (580)	SPalmE:901 Moritz AE et al. (2015)
IFM3_HUMAN	Interferon-induced transmembrane protein 3	Q01628	SPalmE:916 McMichael TM et al. (2017)
STMN2_RAT	Stathmin-2	P21818 (22,24)	SPalmE:1053 Levy AD et al. (2011)
HEMA_I34A0	Hemagglutinin [Cleaved into: Hemagglutinin HA1 chain; Hemagglutinin HA2 chain ]	P03459	SPalmE:1321 Gadalla MR et al. (2020) [] SPalmE:1323 Gadalla MR et al. (2020) []
LSR_MOUSE	Lipolysis-stimulated lipoprotein receptor	Q99KG5	SPalmE:1345 Oda Y et al. (2020) []

Copy in clipboard UniProt feature viewer

Isoform sequence alignment

Tip: Hover over an amino acid to display its position!

Orthologs

Select all Deselect all

UniProt AC	UniProt ID	Orthology source	Organism	Gene names	Protein name	Palmitome articles	Times validated	Techniques	Sites	Predicted
Q0VC89	ZDH20_BOVIN	OrthoDB	B. taurus	ZDHHC20	Palmitoyltransferase ZDHHC20, EC 2.3.1.225	0/2	0	0	0	Yes
J9P566	J9P566_CANLF	Ensembl, InParanoid	C. familiaris	LOC100687370	Palmitoyltransferase	0/1	0	0	0	Yes
F1PU04	F1PU04_CANLF	Ensembl, OMA	C. familiaris	ZDHHC2	Palmitoyltransferase	0/1	0	0	0	No
F1PRY6	F1PRY6_CANLF	Ensembl	C. familiaris	ZDHHC2	Uncharacterized protein	0/1	0	0	0	No
G3MXF3	G3MXF3_BOVIN	MetaPhors, Ensembl, OMA, InParanoid	B. taurus	ZDHHC2	Palmitoyltransferase	0/2	0	0	0	Yes
A1ZAR2	A1ZAR2_DROME	MetaPhors, Ensembl	D. melanogaster	Dmel\CG17287, Dmel_CG17287, CG17287	Palmitoyltransferase	0/1	0	0	0	Yes
E2QCN5	E2QCN5_DROME	Ensembl, InParanoid	D. melanogaster	Dmel\CG1407, Dmel_CG1407, CG1407	Palmitoyltransferase	0/1	0	0	0	Yes
Q7K2V5	Q7K2V5_DROME	Ensembl, InParanoid, OMA	D. melanogaster	Dmel\CG1407, Dmel_CG1407, CG1407	Palmitoyltransferase	0/1	0	0	0	Yes
Q8VYP5	ZDH14_ARATH	MetaPhors, OMA	A. thaliana	PAT14, T4C21_210, At3g60800	Probable protein S-acyltransferase 14, EC 2.3.1.225	0/1	1	0	1	Yes
Q5M757	ZDH15_ARATH	OMA, MetaPhors, InParanoid	A. thaliana	PAT12, T18A10.14, A_TM018A10.8, At4g00840	Probable protein S-acyltransferase 12, EC 2.3.1.225	0/1	0	0	0	Yes
Q94C49	ZDH18_ARATH	InParanoid	A. thaliana	PAT13, T12H17.2, T12H17.140, At4g22750	Probable protein S-acyltransferase 13, EC 2.3.1.225	1/1	0	1	0	Yes
P42836	PFA3_YEAST	InParanoid	S. cerevisiae S288c	N0325, YNL326C, PFA3	Palmitoyltransferase PFA3, EC 2.3.1.225	0/1	0	0	0	No
Q8IJC5	Q8IJC5_PLAF7	InParanoid	P. falciparum	PF10_0273	Palmitoyltransferase	0/1	0	0	0	Yes
Q8I3I3	Q8I3I3_PLAF7	InParanoid	P. falciparum	PF3D7_0528400	Palmitoyltransferase	0/1	0	0	0	Yes
O14345	PFA3_SCHPO	InParanoid	S. pombe	SPBC2F12.15c, pfa3	Palmitoyltransferase pfa3	0/1	0	0	0	Yes
P59267	ZDHC2_MOUSE	MetaPhors, OrthoDB, Ensembl, InParanoid, OMA	M. musculus	Zdhhc2	Palmitoyltransferase ZDHHC2, EC 2.3.1.225	0/23	0	0	0	No
Q9JKR5	ZDHC2_RAT	MetaPhors, OrthoDB, Ensembl, InParanoid, OMA	R. norvegicus	Srec, Zdhhc2	Palmitoyltransferase ZDHHC2, EC 2.3.1.225	0/3	0	0	0	No
E2RKX3	E2RKX3_CANLF	OrthoDB	C. familiaris	ZDHHC20	Palmitoyltransferase	0/1	0	0	0	Yes
F1MPF2	F1MPF2_BOVIN	OrthoDB, Ensembl	B. taurus	ZDHHC2	Palmitoyltransferase	0/2	0	0	0	Yes
A1Z833	A1Z833_DROME	MetaPhors, OrthoDB, Ensembl, InParanoid	D. melanogaster	Dmel\CG1407, Dmel_CG1407, CG1407	Palmitoyltransferase	0/1	0	0	0	Yes
Q960T3	Q960T3_DROME	InParanoid	D. melanogaster	CG1407	Palmitoyltransferase	0/1	0	0	0	Yes

Ortholog sequence alignment (CLUSTALW)

Validation experiments

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)
SPalmE:67	Shmueli A et al.	2010	Homo sapiens	HEK293	NA	PAT overexpression
SPalmE:141	Fernández-Hernando C et al.	2006	Homo sapiens	EA.hy 926	Total cell lysate	Depletion of PAT by siRNA, [3H]palmitic acid labeling
SPalmE:148	Mill P et al.	2009	Homo sapiens	HEK293	NA	PAT overexpression, Point mutation, [3H]palmitic acid labeling
SPalmE:149	Sharma C et al.	2008	Homo sapiens	HEK293	NA	Depletion of PAT by siRNA, PAT localization by fluorescent tag, PAT overexpression, PAT point mutation, [3H]palmitic acid labeling
SPalmE:150	Sharma C et al.	2008	Homo sapiens	A431	NA	PAT overexpression, PAT point mutation, [3H]palmitic acid labeling
SPalmE:154	Zhang J et al.	2008	Homo sapiens	HeLa	NA	Depletion of PAT by siRNA, IF localization, PICA
SPalmE:161	Singaraja RR et al.	2009	Homo sapiens	Flp-In-293	Total cell lysate	PAT overexpression, [3H]palmitic acid labeling
SPalmE:197	Fairbank M et al.	2012	Chlorocebus aethiops	COS7	Total cell lysate	ABE, PAT overexpression, Point mutation, Substrate truncation
SPalmE:305	Zeidman R et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Depletion of PAT by siRNA, [3H]palmitic acid labeling
SPalmE:410	Woolfrey KM et al.	2015	Chlorocebus aethiops	COS7	Total cell lysate	ABE, Co-IP of PAT and substrate, PAT localization by IF, PAT point mutation, Point mutation
SPalmE:901	Moritz AE et al.	2015	Sus scrofa	LLC-PK1 porcine liver cells	Total cell lysate	ABE, Co-IP of PAT and substrate, NH2OH cleavage of thioester bond
SPalmE:916	McMichael TM et al.	2017	Homo sapiens	HAP1	Total cell lysate	Alk16 Labeling, Depletion of PAT by siRNA
SPalmE:999	Planey SL et al.	2009	Homo sapiens	HeLa	Plasma Membrane	Depletion of PAT by siRNA, IF localization, Point mutation
SPalmE:1000	Planey SL et al.	2009	Homo sapiens	normal primary bladder epithelial cells	Plasma Membrane	Depletion of PAT by siRNA, IF localization, Point mutation
SPalmE:1053	Levy AD et al.	2011	Rattus norvegicus	HEK293T	Total cell lysate	PAT overexpression, [3H]palmitic acid labeling
SPalmE:1088	Zeidman R et al.	2011	Homo sapiens	HEK293T	Total cell lysate	PAT overexpression, [3H]palmitic acid labeling
SPalmE:1308	Sada R et al.	2019	Homo sapiens	HEK293T	Reticulum endoplasmic	APE, PAT overexpression
SPalmE:1321	Gadalla MR et al.	2019	Homo sapiens	HAP1	Viral fraction	ARAC, Depletion of PAT by siRNA, NH2OH cleavage of thioester bond, [3H]palmitic acid labeling
SPalmE:1323	Gadalla MR et al.	2019	Homo sapiens	HeLa	Viral fraction	Depletion of PAT by siRNA, NH2OH cleavage of thioester bond, [3H]palmitic acid labeling
SPalmE:1345	Oda Y et al.	2020	Mus musculus	HEK293T	Total cell lysate	NH2OH cleavage of thioester bond, PAT overexpression, [3H]palmitic acid labeling
SPalmE:1405	Harada T et al.	2020	Homo sapiens	Lenti-X 293T (BOSC-23)	Total cell lysate	APE, Depletion of PAT by siRNA, NH2OH cleavage of thioester bond, Point mutation, Substrate truncation

Cysteine summary and predictions

Pos in alignment	14	42	55	129	132	140	143	146	149	157	163	183	233	291
Subcell. location	C	E	T	C	C	C	C	C	C	C	C	T	C	C
Disulfide	no	no	no	no	no	no	no	no	no	no	no	no	no	no
PSIPRED v.4.02 prediction	helix	helix	helix	coil	coil	coil	coil	coil	strand	coil	coil	helix	helix	coil
UniProt secondary structure	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys¹⁴ HC LC	Cys⁴²	Cys⁵⁵	Cys¹²⁹	Cys¹³²	Cys¹⁴⁰	Cys¹⁴³	Cys¹⁴⁶	Cys¹⁴⁹ MC	Cys¹⁵⁷	Cys¹⁶³ MC	Cys¹⁸³	Cys²³³	Cys²⁹¹

Pos in alignment	296	352
Subcell. location	C	C
Disulfide	no	no
PSIPRED v.4.02 prediction	coil	coil
UniProt secondary structure	NA	NA
Pos in isoform 1 [Main] CSS-Palm 4.0 PalmPred	Cys²⁹⁶	Cys³⁵²

Legend

Subcell. location

U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear

Prediction confidence

Cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) and validated (in terms of disulfide bonds and cellular location) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
PalmPred: High confidence (score > 0.4), Medium confidence (0.1 < score < 0.4), Low confidence (score < 0.1), Not predicted.

Palmitoyl-proteome experiments

List of palmitoyl-proteomes

Tip: Keep CTRL key pressed down while clicking on column headers to do multiple sorting. This applies to any sortable table on this site.

ID of the experiment	Reference	Year	Organism	Cell type	Subcellular location	Technique(s)	Found?	In list	All lists
SPalmE:14	Roth AF et al.	2006	Saccharomyces cerevisiae	Isogenic LRB759	Total membrane	ABE	x		HC HC: 48 hits
SPalmE:13	Kang R et al.	2008	Rattus norvegicus	Cortical neuron	Synaptosomal membrane	ABE	x		HC: 107 hits LC: 308 hits
SPalmE:6	Martin BR et al.	2009	Homo sapiens	Jurkat	Total membrane	Click chemistry	x		HC: 127 hits LC: 188 hits List: 0 hits
SPalmE:577	Yang W et al.	2010	Homo sapiens	DU145	Non-lipid raft	ABE	x		HC: 63 hits LC: 147 hits
SPalmE:578	Yang W et al.	2010	Homo sapiens	DU145	Lipid raft	ABE	x		HC: 102 hits LC: 73 hits
SPalmE:10	Yount JS et al.	2010	Mus musculus	Dendritic cell	Total cell lysate	Click chemistry	x		HC: 59 hits LC: 105 hits
SPalmE:1	Yang W et al.	2010	Homo sapiens	DU145	Total membrane	ABE	x		HC: 162 hits LC: 218 hits
SPalmE:5	Forrester MT et al.	2011	Homo sapiens	HEK293	Total membrane	ARAC	x		List: 69 hits
SPalmE:8	Merrick BA et al.	2011	Mus musculus	Macrophage Raw 264.7	Total membrane	ABE	x		List: 69 hits
SPalmE:12	Martin BR et al.	2011	Mus musculus	BW5147-derived mouse T-cell hybridoma cell	Total membrane	Click chemistry	x		HC HC: 352 hits LC LC: 8 hits
SPalmE:511	Emmer BT et al.	2011	Trypanosoma brucei brucei	NA	Total cell lysate	ABE	x		List: 126 hits
SPalmE:2	Dowal L et al.	2011	Homo sapiens	Platelet	Total membrane	ABE	x		List: 214 hits
SPalmE:7	Wilson JP et al.	2011	Homo sapiens	Jurkat	Total cell lysate	Click chemistry	x		HC: 164 hits LC: 178 hits
SPalmE:4	Marin EP et al.	2012	Homo sapiens	Umbilical vein blood	NA	ABE	x		List: 154 hits
SPalmE:11	Li Y et al.	2012	Mus musculus	Neural stem cell	Total cell lysate	Click chemistry	x		HC HC: 254 hits
SPalmE:162	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	ABE	x		HC: 100 hits LC: 217 hits
SPalmE:580	Jones ML et al.	2012	Plasmodium falciparum	Whole organism	Total cell lysate	Click chemistry, SILAC	x		HC: 79 hits LC: 89 hits
SPalmE:3	Ivaldi C et al.	2012	Homo sapiens	B-cell	Total membrane	ABE	x		HC HC: 93 hits
SPalmE:119	Wan J et al.	2013	Mus musculus	Brain	Total cell lysate	ABE	x		List: 378 hits
SPalmE:160	Zhang MM et al.	2013	Schizosaccharomyces pombe	Whole organism	Total cell lysate	Click chemistry	x		List: 238 hits
SPalmE:9	Ren W et al.	2013	Mus musculus	3T3-L1 and epididymal fat pads	Total membrane	ARAC	x		List: 664 hits
SPalmE:15	Hemsley PA et al.	2013	Arabidopsis thaliana	Heyn Col-4 ecotype root cell suspension	Microsomal membrane	ABE	Ortholog	in LC [ZDH18]	HC: 122 hits MC: 94 hits LC: 366 hits
SPalmE:184	Wei X et al.	2014	Homo sapiens	HUVEC	Total cell lysate	ABE	x		Palm decreased: 40 hits Palm increased: 33 hits List: 367 hits
SPalmE:507	Chesarino NM et al.	2014	Mus musculus	DC2.4	Total cell lysate	Click chemistry	x		List: 246 hits
SPalmE:508	Chesarino NM et al.	2014	Mus musculus	MEF	Total cell lysate	Click chemistry	x		List: 436 hits
SPalmE:504	Serwa RA et al.	2015	Homo sapiens	RPE-1	Total cell lysate	SILAC, 16-HDYA labeling	Found	in List	List: 1348 hits
SPalmE:505	Morrison E et al.	2015	Homo sapiens	Primary CD4 + T cells	Total cell lysate	ABE, In gel SILAC labeling	x		List: 274 hits
SPalmE:506	Morrison E et al.	2015	Homo sapiens	Jurkat	Total cell lysate	Click chemistry, SILAC	x		List: 226 hits
SPalmE:510	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 354 hits
SPalmE:529	Colquhoun DR et al.	2015	Homo sapiens	174 x CEM.T1	Total cell lysate	Click-IT Palmitic Acid labeling	x		List: 113 hits
SPalmE:536	Foe IT et al.	2015	Toxoplasma gondii	NA	Total membrane	Click chemistry	x		HC: 210 hits MC: 72 hits
SPalmE:540	Gould NS et al.	2015	Mus musculus	Liver	Total cell lysate	APE	x		List: 780 hits
SPalmE:619	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Alk16 Labeling	x		List: 354 hits
SPalmE:620	Peng T et al.	2015	Homo sapiens	HEK293	Total cell lysate	Photo cleavable x-alk16 labeling	x		List: 354 hits
SPalmE:509	Santiago-Tirado FH et al.	2015	Cryptococcus neoformans	NA	Total cell lysate	Click chemistry	x		List: 426 hits
SPalmE:698	Pinner AL et al.	2016	Homo sapiens	Brain	Total cell lysate	ABE	x		List: 216 hits
SPalmE:723	Hernandez JL et al.	2016	Homo sapiens	MCF7	Total cell lysate	Alk16 Labeling	x		List: 1353 hits
SPalmE:724	Fang C et al.	2016	Homo sapiens	SW480	NA	ARAC	x		List: 100 hits
SPalmE:829	Upadhyay S et al.	2016	Aspergillus fumigatus	Conidia	NA	ABE	x		List: 133 hits
SPalmE:902	Caballero MC et al.	2016	Toxoplasma gondii	whole parasite lysates	NA	ABE	x		HC: 164 hits
SPalmE:909	Thinon E et al.	2016	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Alk16 Labeling	x		HC: 197 hits MC: 143 hits LC: 302 hits
SPalmE:855	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		List: 865 hits
SPalmE:903	Collins MO et al.	2017	Mus musculus	Forebrain	NA	ABE	x		HC: 1079 hits
SPalmE:906	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ABE	x		List: 193 hits
SPalmE:907	Edmonds MJ et al.	2017	Rattus norvegicus	Brain	NA	ARAC	x		List: 95 hits
SPalmE:993	Shen LF et al.	2017	Mus musculus	Liver	Total membrane	ARAC	x		ZDHHC13 substrates: 16 hits
SPalmE:908	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		Palm. up with LPS: 156 hits Palm. down with LPS: 189 hits
SPalmE:919	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		List: 646 hits
SPalmE:920	Sobocińska J et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	Click chemistry	x		LPS treatment: 646 hits
SPalmE:1181	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 244 hits MC: 187 hits LC: 120 hits
SPalmE:1182	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 198 hits MC: 143 hits LC: 120 hits
SPalmE:1183	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 132 hits MC: 138 hits LC: 110 hits
SPalmE:1184	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	ARAC	x		HC: 545 hits MC: 415 hits LC: 219 hits
SPalmE:1185	Thinon E et al.	2018	Homo sapiens	HeLa	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, Alk16 Labeling	x		Chymotrypsin: 286 hits Trypsin: 1291 hits
SPalmE:1186	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	NH2OH cleavage of thioester bond, Alk16 Labeling	x		HC: 177 hits MC: 162 hits LC: 123 hits
SPalmE:1187	Thinon E et al.	2018	Mus musculus	Macrophage Raw 264.7	Total cell lysate	ARAC	x		HC: 281 hits MC: 139 hits LC: 113 hits
SPalmE:1191	Won SJ et al.	2018	Homo sapiens	HEK293T	Total cell lysate	Click chemistry	x		List: 195 hits
SPalmE:1192	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	Click chemistry	x		List: 984 hits
SPalmE:1193	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC	x		List: 1394 hits
SPalmE:1194	Won SJ et al.	2018	Homo sapiens	HAP1	Total cell lysate	ARAC,	x		List: 1404 hits
SPalmE:1203	Zhang X et al.	2018	Mus musculus	Brain	Total membrane	Enrichment by magnetic nanoparticles	x		HC: 917 hits MC: 352 hits LC: 15 hits
SPalmE:1259	Dumoulin A et al.	2018	Mus musculus	F11	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 1 hits MC: 10 hits LC: 4 hits
SPalmE:1260	Wang Z et al.	2018	Bos taurus	Lens fiber cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 52 hits
SPalmE:1265	Wang J et al.	2019	Mus musculus	3T3-L1	Total membrane	MS, BioID	x		List: 9 hits
SPalmE:1266	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 157 hits
SPalmE:1270	Strassburger K et al.	2019	Drosophila melanogaster	Circa 160 L2 larvae	Total cell lysate	ABE, NH2OH cleavage of thioester bond, PAT KO, MS	x		List: 13 hits
SPalmE:1303	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1333	Zaręba-Kozioł M et al.	2019	Mus musculus	Brain	Postsynaptic density fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x
SPalmE:1432	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	ABE, SILAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 590 hits
SPalmE:1433	Zhou B et al.	2019	Homo sapiens	LNCaP	Total cell lysate	NH2OH cleavage of thioester bond, LC-MS, LB-ABE, ABE	Found	in List	List: 2929 hits
SPalmE:1434	Wegleiter T et al.	2019	Mus musculus	Neural stem cell	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 370 hits
SPalmE:1458	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Stimulated: 148 hits
SPalmE:1435	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		Unstimulated: 37 hits
SPalmE:1436	Morrison E et al.	2020	Homo sapiens	Jurkat	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS, SILAC	x		List: 185 hits
SPalmE:1437	Mariscal J et al.	2020	Homo sapiens	PC3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1869 hits
SPalmE:1438	Mariscal J et al.	2020	Homo sapiens	PC3	Total membrane	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	Found	in HC	HC: 1837 hits
SPalmE:1439	Mariscal J et al.	2020	Homo sapiens	PC3	Large Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 1350 hits
SPalmE:1440	Mariscal J et al.	2020	Homo sapiens	PC3	Small Extracellular Vesicles	ARAC, NH2OH cleavage of thioester bond, LC-MS, LB-ABE	x		HC: 600 hits
SPalmE:1454	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 675 hits MC: 360 hits LC: 27 hits
SPalmE:1455	Ziemlińska E et al.	2021	Mus musculus	Liver	Total membrane	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 850 hits MC: 303 hits LC: 21 hits
SPalmE:1443	Uzbekova S et al.	2021	Bos taurus	Bovine follicular cells	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 80 hits
SPalmE:1444	Miles MR et al.	2021	Homo sapiens	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 687 hits
SPalmE:1445	Miles MR et al.	2021	Rattus norvegicus	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 399 hits
SPalmE:1446	Miles MR et al.	2021	Canis familiaris	Heart	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 464 hits
SPalmE:1447	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 1173 hits
SPalmE:1448	Pradhan AJ et al.	2021	Homo sapiens	HT-29	Total cell lysate	Labeling of substrate with C20 AlkFA (Alk-18), LC-MS	x		List: 770 hits
SPalmE:1449	Ripamonti M et al.	2021	Mus musculus	NIH-3T3	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 199 hits
SPalmE:1450	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1093 hits
SPalmE:1451	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1048 hits
SPalmE:1452	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1112 hits
SPalmE:1453	Zaręba-Kozioł M et al.	2021	Mus musculus	Brain	Synaptoneurosomal fraction	ABE, NH2OH cleavage of thioester bond, LC-MS	x		List: 1040 hits
SPalmE:1456	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 4107 hits
SPalmE:1457	Gao J et al.	2022	Mus musculus	Testis	Total cell lysate	ABE, NH2OH cleavage of thioester bond, LC-MS	x		HC: 3870 hits
SPalmE:1441	Gorenberg EL et al.	2022	Mus musculus	Brain	Synaptic fraction	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1378 hits
SPalmE:1442	Gorenberg EL et al.	2022	Mus musculus	Brain	Total cell lysate	ARAC, NH2OH cleavage of thioester bond, LC-MS	x		List: 1597 hits

Summary table by organism

Organism	Total number of palmitoyl-proteome articles	Total number of palmitoyl-proteomes	Number of palmitoyl-proteomes containing this protein or an ortholog	% of palmitoyl-proteomes containing this protein or an ortholog	Orthologs
S. cerevisiae S288c	1	1	0	0.0	NA
R. norvegicus	3	4	0	0.0	NA
H. sapiens	22	38	4	10.5	NA
M. musculus	23	37	0	0.0	NA
T. brucei brucei	1	1	0	0.0	NA
P. falciparum	1	2	0	0.0	NA
S. pombe	1	1	0	0.0	NA
A. thaliana	1	1	1	100.0	ZDH18_ARATH
C. neoformans var. grubii serotype A strain H99	1	2	0	0.0	NA
T. gondii	2	2	0	0.0	NA
A. fumigatus	1	1	0	0.0	NA
B. taurus	2	2	0	0.0	NA
D. melanogaster	1	2	0	0.0	A1Z833_DROME , E2QCN5_DROME
C. familiaris	1	1	0	0.0	NA

References

Authors	Year	Reference title	PMID
Edmonds MJ et al.	2017	Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.	28607426
Collins MO et al.	2017	Global, site-specific analysis of neuronal protein S-acylation.	28680068
Colquhoun DR et al.	2015	Bioorthogonal mimetics of palmitoyl-CoA and myristoyl-CoA and their subsequent isolation by click chemistry and characterization by mass spectrometry reveal novel acylated host-proteins modified by HIV-1 infection.	25914232
Li Y et al.	2012	DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.	22081607
Thinon E et al.	2016	Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.	27350074
Kang R et al.	2008	Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation.	19092927
Wang J et al.	2019	DHHC4 and DHHC5 Facilitate Fatty Acid Uptake by Palmitoylating and Targeting CD36 to the Plasma Membrane.	30605677
Harada T et al.	2020	Palmitoylated CKAP4 regulates mitochondrial functions through an interaction with VDAC2 at ER-mitochondria contact sites.	33067255
Yang W et al.	2010	Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes.	19801377
Zaręba-Kozioł M et al.	2021	S-Palmitoylation of Synaptic Proteins as a Novel Mechanism Underlying Sex-Dependent Differences in Neuronal Plasticity.	34200797
Woolfrey KM et al.	2015	The palmitoyl acyltransferase DHHC2 regulates recycling endosome exocytosis and synaptic potentiation through palmitoylation of AKAP79/150.	25589740
Upadhyay S et al.	2016	Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation.	27879337
Won SJ et al.	2018	Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.	29733200
Wegleiter T et al.	2019	Palmitoylation of BMPR1a regulates neural stem cell fate.	31772009
Shmueli A et al.	2010	Ndel1 palmitoylation: a new mean to regulate cytoplasmic dynein activity.	19927128
Pinner AL et al.	2016	Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia.	26876311
Zhang J et al.	2008	Identification of CKAP4/p63 as a major substrate of the palmitoyl acyltransferase DHHC2, a putative tumor suppressor, using a novel proteomics method.	18296695
Mariscal J et al.	2020	Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles.	32944167
Sharma C et al.	2008	DHHC2 affects palmitoylation, stability, and functions of tetraspanins CD9 and CD151.	18508921
Fairbank M et al.	2012	RING finger palmitoylation of the endoplasmic reticulum Gp78 E3 ubiquitin ligase.	22728137
Dowal L et al.	2011	Proteomic analysis of palmitoylated platelet proteins.	21813449
Martin BR et al.	2009	Large-scale profiling of protein palmitoylation in mammalian cells.	19137006
Chesarino NM et al.	2014	Chemoproteomics reveals Toll-like receptor fatty acylation.	25371237
Ivaldi C et al.	2012	Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.	22615937
Zhou B et al.	2019	Low-background Acyl-biotinyl Exchange Largely Eliminates the Co-isolation of Non-S-acylated Proteins and Enables Deep S-acylproteomic Analysis.	31251020
Caballero MC et al.	2016	Identification of new palmitoylated proteins in Toxoplasma gondii.	26825284
Gadalla MR et al.	2019	Hemagglutinin of Influenza A, but not of Influenza B and C viruses is acylated by ZDHHC2, 8, 15 and 20.	31872235
Miles MR et al.	2021	Global identification of S-palmitoylated proteins and detection of palmitoylating (DHHC) enzymes in heart.	33636221
Wei X et al.	2014	Insulin-regulated protein palmitoylation impacts endothelial cell function.	24357059
Dumoulin A et al.	2018	S-palmitoylation Is Required for the Control of Growth Cone Morphology of DRG Neurons by CNP-Induced cGMP Signaling.	30319353
Forrester MT et al.	2011	Site-specific analysis of protein S-acylation by resin-assisted capture.	21044946
Zhang MM et al.	2013	Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.	23843742
Planey SL et al.	2009	Palmitoylation of cytoskeleton associated protein 4 by DHHC2 regulates antiproliferative factor-mediated signaling.	19144824
Zhang X et al.	2018	Ultradeep Palmitoylomics Enabled by Dithiodipyridine-Functionalized Magnetic Nanoparticles.	29660268
Peng T et al.	2015	Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.	25575299
Gao J et al.	2022	Proteome-wide identification of palmitoylated proteins in mouse testis.	35477839
Wan J et al.	2013	Tracking brain palmitoylation change: predominance of glial change in a mouse model of Huntington's disease.	24211138
Zeidman R et al.	2011	DHHC2 is a protein S-acyltransferase for Lck.	22034844
Shen LF et al.	2017	Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.	28526873
Moritz AE et al.	2015	Reciprocal Phosphorylation and Palmitoylation Control Dopamine Transporter Kinetics.	26424792
Pradhan AJ et al.	2021	Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.	33848465
Morrison E et al.	2020	Dynamic palmitoylation events following T-cell receptor signaling.	32651440
Sobocińska J et al.	2018	Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.	29217618
Yount JS et al.	2010	Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.	20601941
Santiago-Tirado FH et al.	2015	A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.	25970403
Wang Z et al.	2018	Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20.	30489624
Wilson JP et al.	2011	Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.	21076176
Mill P et al.	2009	Palmitoylation regulates epidermal homeostasis and hair follicle differentiation.	19956733
Levy AD et al.	2011	Subcellular Golgi localization of stathmin family proteins is promoted by a specific set of DHHC palmitoyl transferases.	21471001
Strassburger K et al.	2019	Drosophila ZDHHC8 palmitoylates scribble and Ras64B and controls growth and viability.	30735487
Martin BR et al.	2011	Global profiling of dynamic protein palmitoylation.	22056678
Ziemlińska E et al.	2021	Palm Oil-Rich Diet Affects Murine Liver Proteome and <i>S</i>-Palmitoylome.	34884899
Jones ML et al.	2012	Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis.	22901544
Foe IT et al.	2015	Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.	26468752
Gould NS et al.	2015	Site-Specific Proteomic Mapping Identifies Selectively Modified Regulatory Cysteine Residues in Functionally Distinct Protein Networks.	26165157
Fernández-Hernando C et al.	2006	Identification of Golgi-localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase.	16864653
Serwa RA et al.	2015	Systems Analysis of Protein Fatty Acylation in Herpes Simplex Virus-Infected Cells Using Chemical Proteomics.	26256475
Singaraja RR et al.	2009	Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function.	19556522
Hernandez JL et al.	2016	Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.	27030425
Zaręba-Kozioł M et al.	2019	Stress-induced changes in the S-palmitoylation and S-nitrosylation of synaptic proteins.	31311849
Thinon E et al.	2018	Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.	29575903
Uzbekova S et al.	2021	Protein Palmitoylation in Bovine Ovarian Follicle.	34769186
Ripamonti M et al.	2021	Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions.	33782527
Morrison E et al.	2015	Quantitative analysis of the human T cell palmitome.	26111759
Marin EP et al.	2012	Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases.	22496122
Merrick BA et al.	2011	Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3.	21785166
Emmer BT et al.	2011	Global analysis of protein palmitoylation in African trypanosomes.	21193548
Ren W et al.	2013	Proteomic analysis of protein palmitoylation in adipocytes.	23599907
Gorenberg EL et al.	2022	Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.	35358180
Fang C et al.	2016	Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody.	26865113
Roth AF et al.	2006	Global analysis of protein palmitoylation in yeast.	16751107
Sada R et al.	2019	Dynamic palmitoylation controls the microdomain localization of the DKK1 receptors CKAP4 and LRP6.	31744930
Oda Y et al.	2020	The extracellular domain of angulin-1 and palmitoylation of its cytoplasmic region are required for angulin-1 assembly at tricellular contacts.	32079676
McMichael TM et al.	2017	The palmitoyltransferase ZDHHC20 enhances interferon-induced transmembrane protein 3 (IFITM3) palmitoylation and antiviral activity.	29079573
Hemsley PA et al.	2013	A proteomic approach identifies many novel palmitoylated proteins in Arabidopsis.	23252521

Q9UIJ5 ZDHC2_HUMAN