Editing Study

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Title
Authors
Year
Organism
Cell type
Subcellular fraction
Techniques
ABE (Acyl-Biotin Exchange)
Acyl PEG Exchange (APE)
Acyl RAC
Acyl phenylmercury enrichment
Acyl-RadioactiveNEM exchange
Biotin Switch Assay
Capture of palmitoylated cysteines with iodoacetamide (IAA)
Cellular localization of palmitoyltransferase by fusion with fluorescent protein
Cellular localization of palmitoyltransferase by immunofluorescence
Cellular localization of substrate by fusion with fluorescent protein
Cellular localization of substrate by immunofluorescence
Cellular localization of thioesterase by fusion with a fluorescent protein
Cleavage of thioester bonds with DTT
Cleavage of thioester bonds with KOH
Cleavage of thioester bonds with NH2OH
Cleavage of thioester bonds with β-mercaptoethanol
Co-immunoprecipitation of palmitoyltransferase and substrate
Co-precipitation of palmitoyltransferase and substrate
Deletion of part of substrate
Depalmitoylation by incubation with protein thioesterase
Depletion of palmitoyltransferase using shRNA
Depletion of palmitoyltransferase using siRNA
Depletion of palmitoyltransferase with sgRNA
Depletion of thioesterase with shRNA
Depletion of thioesterase with siRNA
Gas chromatography
High-Performance Liquid Chromatography (HPLC)
Immunoprecipitation acyl-release assay
In gel SILAC labelling with H2(18)O and H2(16)O
Inhibition of palmitoylation by C75 FA synthase inhibitor
Inhibition of palmitoylation by cerulenin
Inhibition of palmitoylation with 13-oxypalmitate
Inhibition of palmitoylation with 2-bromopalmitate
Inhibition of palmitoylation with 2-fluoropalmitate
Inhibition of palmitoyltransferase with drug
Inhibition of thioesterase with ML348 or ML349
Inhibition of thioesterase with drug
Inhibition of thioesterase with hexadecylfluorophosphonate (HDFP)
Knock-out of palmitoyltransferase
Knockdown of esterase
Labeling of substrate with 15-HDYA (15-hexadecynoic acid = alk-14)
Labeling of substrate with [125I]-IC16 palmitate
Labeling of substrate with 13-alkyne Myristic acid ( =alk12 )
Labeling of substrate with 14-PDYA (14-pentadecynoic acid)
Labeling of substrate with 15-Azidopentadecanoic Acid (Click-IT Palmitic Acid)
Labeling of substrate with 15-Hexadecynoyl-CoA
Labeling of substrate with 16-[125I]iodohexadecanoic acid
Labeling of substrate with 17-ODYA (17-octadecynoic acid)
Labeling of substrate with [14C]iodoacetamide treatment
Labeling of substrate with [14C]palmitic acid
Labeling of substrate with [35S]-Cysteine
Labeling of substrate with [3H]palmitic acid
Labeling of substrate with [3H]palmitoleic acid
Labeling of substrate with [3H]palmitoyl-CoA
Labeling of substrate with alk16
Labeling of substrate with photo cleavable x-alk16
Labelling of substrate with 1-[14C]palmitoyl-CoA
Labelling of substrate with 14-carbon myristoylation (Alk-C14)
Labelling of substrate with 16-HDYA (16-heptadecynoic acid)
Labelling of substrate with 18-carbon monounsaturated palmitoleic acid (Alk-C18:1)
Labelling of substrate with 18-carbon stearoylation (Alk-C18)
Labelling of substrate with 20-carbon arachidic acid (Alk-C20)
Labelling of substrate with [125I]iodopalmitoyl-CoA
Labelling of substrate with az12
Labelling of substrate with biotin-modified palmiate
Labelling of substrate with cis-9-hexadecen-15-ynoic acid (Alk-C16:1)
Labelling of substrate with palmitoyl-CoA
Labelling with Azidotetradecanoic acid
Liquid Chromatography
Liquid Chromatography coupled to Mass Spectrometry
Mass Spectrometry
Over-expression of Acyl-Protein Thioesterase
Over-expression of dominant-negative palmitoyltransferase
Over-expression of palmitoyltransferase
PICA (Palmitoyl-cysteine Isolation Capture and Analysis)
Point mutation of esterase
Point mutation of palmitoyltransferase
Point mutation of substrate
Reverse-phase chromatography
SILAC
SILAC labelling with light arginine and lysine or heavy-labeled 13C6,15N4-arginine and 13C6-lysine
Thin-layer chromatography
Thiopropyl captivation
Treatment with N-( tert-Butyl) hydroxylamine
Truncation of palmitoyltransferase
Truncation of substrate
Two hybrid assay with Acyl-Protein Thioesterase and substrate
Two-hybrid assay with palmitoyltransferase and substrate
azido-palmitate (C14N3)
inhibition of thioesterase with palmostatin B
over expression of substrate
Type of studyTargeted
In vitro
Internal study (not public)
Name (for internal studies)
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